scholarly journals Host Plant-Derived miRNAs Potentially Modulate the Development of a Cosmopolitan Insect Pest, Plutella xylostella

Biomolecules ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 602 ◽  
Author(s):  
Ling-ling Zhang ◽  
Xiao-dong Jing ◽  
Wei Chen ◽  
Yue Wang ◽  
Jun-han Lin ◽  
...  
Keyword(s):  
Host Plant ◽  
Plutella Xylostella ◽  
Target Genes ◽  
Insect Pest ◽  
Circulatory System ◽  
Insect Herbivore ◽  
Hatching Rate ◽  
Egg Hatching ◽  
Physiological Processes ◽  
The Cross

Plant microRNAs (miRNAs) have recently been reported to be involved in the cross-kingdom regulation of specific cellular and physiological processes in animals. However, little of this phenomenon is known for the communication between host plant and insect herbivore. In this study, the plant-derived miRNAs in the hemolymph of a cruciferous specialist Plutella xylostella were identified by small RNAs sequencing. A total of 39 miRNAs with typical characteristics of plant miRNAs were detected, of which 24 had read counts ≥ 2 in each library. Three plant-derived miRNAs with the highest read counts were validated, and all of them were predicted to target the hemocyanin domains-containing genes of P. xylostella. The luciferase assays in the Drosophila S2 cell demonstrated that miR159a and novel-7703-5p could target BJHSP1 and PPO2 respectively, possibly in an incomplete complementary pairing mode. We further found that treatment with agomir-7703-5p significantly influenced the pupal development and egg-hatching rate when reared on the artificial diet. The developments of both pupae and adults were severely affected upon their transfer to Arabidopsis thaliana, but this might be independent of the cross-kingdom regulation of the three plant-derived miRNAs on their target genes in P. xylostella, based on expression analysis. Taken together, our work reveals that the plant-derived miRNAs could break the barrier of the insect mid-gut to enter the circulatory system, and potentially regulate the development of P. xylostella. Our findings provide new insights into the co-evolution of insect herbivore and host plant, and novel direction for pest control using plant-derived miRNAs.

scholarly journals Toxicity and Sublethal Effect of Farnesyl Acetate on Diamondback Moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae)

Insects ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 109
Author(s):  
Norazila Yusoff ◽  
Idris Abd Ghani ◽  
Nurul Wahida Othman ◽  
Wan Mohd Aizat ◽  
Maizom Hassan
Keyword(s):  
Plutella Xylostella ◽  
Sublethal Effects ◽  
Diamondback Moth ◽  
Sublethal Effect ◽  
Sublethal Concentration ◽  
Pupal Weight ◽  
Control Group ◽  
Hatching Rate ◽  
Egg Hatching ◽  
Female Ratio

The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is the most important pest of cruciferous vegetables worldwide. In this study, we evaluated the properties of selected farnesyl derivative compounds against P. xylostella. The toxicity and sublethal concentration (LC50) of farnesyl acetate, farnesyl acetone, farnesyl bromide, farnesyl chloride, and hexahydrofarnesyl acetone were investigated for 96 h. The leaf-dip bioassays showed that farnesyl acetate had a high level of toxicity against P. xylostella compared to other tested farnesyl derivatives. The LC50 value was 56.41 mg/L on the second-instar larvae of P. xylostella. Then, the sublethal effects of farnesyl acetate on biological parameters of P. xylostella were assessed. Compared to the control group, the sublethal concentration of farnesyl acetate decreased pupation and emergence rates, pupal weight, fecundity, egg hatching rate, female ratio, and oviposition period. Furthermore, the developmental time of P. xylostella was extended after being exposed to farnesyl acetate. Moreover, the application of farnesyl acetate on P. xylostella induced morphogenetic abnormalities in larval–pupal intermediates, adults that emerged with twisted wings, or complete adults that could not emerge from the cocoon. These results suggested that farnesyl acetate was highly effective against P. xylostella. The sublethal concentration of farnesyl acetate could reduce the population of P. xylostella by increasing abnormal pupal and adults, and by delaying its development period.

Effect of Temperature on the Predator, (Reuter) (Hemiptera: Anthocordiae) Feeding on Cryptolestes pusillus (Schon.) (Coleoptera: Cucujidae)

1970 ◽  
Vol 15 ◽  
pp. 41-46 ◽  
Author(s):  
MM Rahman ◽  
W Islam ◽  
KN Ahmed
Keyword(s):  
Life History ◽  
Insect Pest ◽  
Developmental Time ◽  
Effect Of Temperature ◽  
Hatching Rate ◽  
Immature Stages ◽  
Egg Hatching ◽  
Influence Of Temperature ◽  
Influence Of Temperature On ◽  
Cryptolestes Pusillus

Xylocoris flavipes (Reuter) is one of the dominant predators of many stored product insect pest including Cryptolestes pusillus. The influence of temperature on predator development, survival and some selected life history parameters was determined. Eggs laid/female (27.27±2.52) and egg hatching rate (%) (88.25±2.19) were highest at 30°C and lowest at 20°C (5.43±1.19 and 30.79±4.63%) respectively but no eggs laid at 15°C. Mortality among immature stages (%) was highest (51.71±1.48) at 35°C and lowest (24.25c±1.14) at 25°C. Developmental times decreasing with the increasing of temperature. Maximum numbers of progeny/female/day (3.55±0.76) were produced at 25°C and minimum (0.83±0.04) were at 20°C.The sex ratios (% female) of X. flavipes were 47.04, 56.68, 51.66 and 50.07 for 20, 25, 30 and 35°C respectively. Survivorship of ovipositing females was highest at 25°C but lowest at 35°C respectively. Key words: Xylocoris flavipes, Cryptolestes pusillus, life history, temperature, developmental time   doi: 10.3329/jbs.v15i0.2201 J. bio-sci. 15: 41-46, 2007

scholarly journals Variation in Performance and Resistance to Parasitism of Plutella xylostella Populations

Insects ◽  
2019 ◽  
Vol 10 (9) ◽  
pp. 293
Author(s):  
Rieta Gols ◽  
Gaylord A. Desurmont ◽  
Jeffrey A. Harvey
Keyword(s):  
Host Plant ◽  
Plant Species ◽  
Plutella Xylostella ◽  
Natural Populations ◽  
Ecological Factors ◽  
Adult Emergence ◽  
Insect Herbivore ◽  
Larval Performance ◽  
Development Times ◽  
Pupal Mortality

Two major ecological factors determine the fitness of an insect herbivore: the ability to overcome plant resistance strategies (bottom-up effects) and the ability to avoid or resist attack by natural enemies such as predators and parasitoids (top-down effects). In response to differences in selection pressure, variation may exist in host-plant adaptation and immunity against parasitism among populations of an insect herbivore. We investigated the variation in larval performance of six different Plutella xylostella populations originating from four continents when feeding on a native Dutch plant species, Brassica rapa. One of the used populations has successfully switched its host plant, and is now adapted to pea. In addition, we determined the resistance to attack by the endoparasitoid Diadegma semiclausum originating from the Netherlands (where it is also native) and measured parasitoid performance as a proxy for host resistance against parasitism. Pupal mortality, immature development times, and adult biomass of P. xylostella differed significantly across populations when feeding on the same host plant species. In addition, parasitism success differed in terms of parasitoid adult emergence and their biomass, but not their development times. Variation among natural populations of insects should be considered more when studying interactions between plants and insects up the food chain.

Host-plant switching promotes the population growth of Apolygus lucorum: implications for laboratory rearing

2018 ◽  
Vol 109 (3) ◽  
pp. 309-315 ◽  
Author(s):  
H.-S. Pan ◽  
B. Liu ◽  
Y.-H. Lu
Keyword(s):  
Population Growth ◽  
Host Plant ◽  
Green Bean ◽  
Adult Longevity ◽  
Hatching Rate ◽  
Egg Hatching ◽  
Laboratory Rearing ◽  
Apolygus Lucorum ◽  
Maize Kernels ◽  
Adult Food

AbstractThe mirid bug Apolygus lucorum (Meyer-Dür) (Hemiptera: Miridae) is a major pest on cotton, fruit trees and other crops in China. A. lucorum adults often switch host plants in the agro-ecosystem, and such host-plant switching may promote more rapid population growth of A. lucorum. Here, we examined the population fitness of A. lucorum on different combinations of two plant foods [fresh maize kernels (Zea mays) and green bean pods (Phaseolus vulgaris)] in the laboratory when reared either individually or in groups. Our results suggested that, compared with A. lucorum nymphs reared on green bean alone, the survival rate, developmental rate, and adult weight significantly increased when they were fed fresh maize kernels for both rearing methods. Both two-plant combinations of foods (i.e., maize as nymphal food then green bean as adult food, and green bean as nymphal food then maize as adult food) generally prolonged adult longevity, improved female fecundity, and higher egg hatching rate compared with maize or green bean as food for both nymphs and adults. The combination of nymphs with maize and adults with green bean showed the highest population growth rate for both individual and group rearing of mirid bugs. Host food switching greatly promoted the population growth of A. lucorum, and suggests a new diet for laboratory rearing of A. lucorum.

scholarly journals Spatio-Temporal Profiling of Metarhizium anisopliae—Responsive microRNAs Involved in Modulation of Plutella xylostella Immunity and Development

2021 ◽  
Vol 7 (11) ◽  
pp. 942
Author(s):  
Junaid Zafar ◽  
Yuxin Zhang ◽  
Junlin Huang ◽  
Shoaib Freed ◽  
Rana Fartab Shoukat ◽  
...  
Keyword(s):  
Plutella Xylostella ◽  
Metarhizium Anisopliae ◽  
Molecular Mechanisms ◽  
Target Genes ◽  
Defense Mechanism ◽  
Pathogenic Fungus ◽  
Insect Pest ◽  
Luciferase Assay ◽  
Economic Losses

Metarhizium anisopliae, a ubiquitous pathogenic fungus, regulates a wide array of the insect pest population. The fungus has been employed to control Plutella xylostella, an insecticide-resistant destructive lepidopteran pest, which causes substantial economic losses in crops worldwide. Integration of modern gene-silencing technologies in pest control strategies has become more crucial to counter pesticide-resistant insects. MicroRNAs (miRNA) play essential roles in the various biological process via post-transcriptional gene regulation. In the present study, RNA-seq analysis of control (CK36h, CK72h) and fungal-infected (T36h, T72h) midguts was performed to reveal underlying molecular mechanisms occurring in larval midgut at different time courses. We aimed at exploring M. anisopliae-responsive miRNAs and their target genes involved in development and immunity. After data filtration, a combined set of 170 miRNAs were identified from all libraries. Interestingly, miR-281, miR-263, miR-1, miR-6094 and miR-8 were listed among the most abundantly expressed conserved miRNAs. Furthermore, we experimentally studied the role of differentially expressed miR-11912-5p in regulating corresponding target trypsin-like serine proteinase (Px_TLSP). The luciferase assay (in vitro) revealed that miRNA-11912-5p significantly downregulated its target gene, suggesting it might play a crucial role in defense mechanism of P. xylostella against M.+ anisopliae infection. We used synthetic miRNA mimic/inhibitor (in vivo), to overexpress/silence miRNA, which showed harmful effects on larval duration, survival and adult fecundity. Additionally, fungal application in the presence of mimics revealed enhanced sensitivity of P. xylostella to infection. Our finding provides an insight into the relatively obscure molecular mechanisms involved in insect midgut during the fungal infection.

scholarly journals Histone deacetylase (HDAC) 9: versatile biological functions and emerging roles in human cancer

2021 ◽  
Author(s):  
Chun Yang ◽  
Stéphane Croteau ◽  
Pierre Hardy
Keyword(s):  
Histone Deacetylase ◽  
Posttranslational Modifications ◽  
Histone Deacetylases ◽  
Muscle Development ◽  
Target Genes ◽  
Human Cancer ◽  
Expression Patterns ◽  
Aberrant Expression ◽  
Physiological Processes ◽  
Cancer Pathogenesis

Abstract Background HDAC9 (histone deacetylase 9) belongs to the class IIa family of histone deacetylases. This enzyme can shuttle freely between the nucleus and cytoplasm and promotes tissue-specific transcriptional regulation by interacting with histone and non-histone substrates. HDAC9 plays an essential role in diverse physiological processes including cardiac muscle development, bone formation, adipocyte differentiation and innate immunity. HDAC9 inhibition or activation is therefore a promising avenue for therapeutic intervention in several diseases. HDAC9 overexpression is also common in cancer cells, where HDAC9 alters the expression and activity of numerous relevant proteins involved in carcinogenesis. Conclusions This review summarizes the most recent discoveries regarding HDAC9 as a crucial regulator of specific physiological systems and, more importantly, highlights the diverse spectrum of HDAC9-mediated posttranslational modifications and their contributions to cancer pathogenesis. HDAC9 is a potential novel therapeutic target, and the restoration of aberrant expression patterns observed among HDAC9 target genes and their related signaling pathways may provide opportunities to the design of novel anticancer therapeutic strategies.

scholarly journals On the virulence of the entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae (Ascomycota: Hypocreales), against the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae)

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Muhammad Shehzad ◽  
Muhammad Tariq ◽  
Tariq Mukhtar ◽  
Asim Gulzar
Keyword(s):  
Sex Ratio ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Plutella Xylostella ◽  
Metarhizium Anisopliae ◽  
Insect Pest ◽  
Diamondback Moth ◽  
Economic Damage ◽  
Offspring Sex Ratio ◽  
Development Of Resistance

Abstract Background The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is a noxious pest of cruciferous crops all over the world causing serious economic damage. Management of insect pest generally depends on chemical control; however, due to development of resistance against all types of insecticides, alternative approaches especially utilization of a microbial agent is inevitable. Results Potential of 2 entomopathogenic fungi (EPF), viz., Beauveria bassiana and Metarhizium anisopliae, was evaluated against 2nd and 3rd larval instars of P. xylostella by adopting leaf dip and direct spraying methods under laboratory conditions. Significant mortality rate was achieved by each fungus under adopted methodologies. However, B. bassiana was found to be more effective in both conditions than M. anisopliae. Highest mean corrected mortality (77.80%) was recorded, when spores of B. bassiana were sprayed on the 2nd instar larvae (LC50=1.78×104/ml) after the 6th day of treatment. Similarly, incase of M. anisopliae LC50 for the 2nd instar at the same methodology was 2.78×104/ml with a mortality percentage of 70.0%. Offspring sex ratio was non-significantly related to treatment concentration and methodology, except for the control. Conclusion Beauveria bassiana and M. anisopliae had potential to suppress P. xylostella infestations when applied appropriately. Present findings suggested that B. bassiana and M. anisopliae when sprayed on immatures of host insect had more effect as compared to leaf dip procedure. Furthermore, no significant effect of concentrations was observed on sex ratio.

Chemosensory genes in the head of Spodoptera litura larvae

2021 ◽  
pp. 1-10
Author(s):  
Lu-Lu Li ◽  
Ji-Wei Xu ◽  
Wei-Chen Yao ◽  
Hui-Hui Yang ◽  
Youssef Dewer ◽  
...  
Keyword(s):  
Spodoptera Litura ◽  
Phylogenetic Trees ◽  
Target Genes ◽  
Control Strategies ◽  
Insect Pest ◽  
Management Strategies ◽  
Food Sources ◽  
Larval Feeding ◽  
Chemosensory System ◽  
Chemosensory Genes

Abstract The tobacco cutworm Spodoptera litura (Lepidoptera: Noctuidae) is a polyphagous pest with a highly selective and sensitive chemosensory system involved in complex physiological behaviors such as searching for food sources, feeding, courtship, and oviposition. However, effective management strategies for controlling the insect pest populations under threshold levels are lacking. Therefore, there is an urgent need to formulate eco-friendly pest control strategies based on the disruption of the insect chemosensory system. In this study, we identified 158 putative chemosensory genes based on transcriptomic and genomic data for S. litura, including 45 odorant-binding proteins (OBPs, nine were new), 23 chemosensory proteins (CSPs), 60 odorant receptors (ORs, three were new), and 30 gustatory receptors (GRs, three were new), a number higher than those reported by previous transcriptome studies. Subsequently, we constructed phylogenetic trees based on these genes in moths and analyzed the dynamic expression of various genes in head capsules across larval instars using quantitative real-time polymerase chain reaction. Nine genes–SlitOBP8, SlitOBP9, SlitOBP25, SlitCSP1, SlitCSP7, SlitCSP18, SlitOR34, SlitGR240, and SlitGR242–were highly expressed in the heads of 3- to 5-day-old S. litura larvae. The genes differentially expressed in olfactory organs during larval development might play crucial roles in the chemosensory system of S. litura larvae. Our findings substantially expand the gene inventory for S. litura and present potential target genes for further studies on larval feeding in S. litura.

Molecular and Functional Characterization of One Odorant-Binding Protein Gene OBP3 in Bemisia tabaci (Hemiptera: Aleyrodidae)

2019 ◽  
Author(s):  
Ran Wang ◽  
Yuan Hu ◽  
Peiling Wei ◽  
Cheng Qu ◽  
Chen Luo
Keyword(s):  
Host Plant ◽  
Bemisia Tabaci ◽  
Binding Protein ◽  
Insect Pest ◽  
Critical Role ◽  
Functional Characterization ◽  
Odorant Binding Protein ◽  
Binding Characteristics ◽  
And Function ◽  
Odorant Binding

Abstract Odorant binding proteins (OBPs) of insects play a critical role in chemical perceptions and choice of insect host plant. Bemisia tabaci is a notorious insect pest which can damage more than 600 plant species. In order to explore functions of OBPs in B. tabaci, here we investigated binding characteristics and function of odorant-binding protein 3 in B. tabaci (BtabOBP3). The results indicated that BtabOBP3 shows highly similar sequence with OBPs of other insects, including the typical signature motif of six cysteines. The recombinant BtabOBP3 protein was obtained, and the evaluation of binding affinities to tested volatiles of host plant was conducted, then the results indicated that β-ionone had significantly higher binding to BtabOBP3 among other tested plant volatiles. Furthermore, silencing of BtabOBP3 significantly altered choice behavior of B. tabaci to β-ionone. In conclusion, it has been demonstrated that BtabOBP3 exerts function as one carrier of β-ionone and the results could be contributed to reveal the mechanisms of choosing host plant in B. tabaci.

scholarly journals PPARgene: A Database of Experimentally Verified and Computationally Predicted PPAR Target Genes

PPAR Research ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Li Fang ◽  
Man Zhang ◽  
Yanhui Li ◽  
Yan Liu ◽  
Qinghua Cui ◽  
...  
Keyword(s):  
In Silico ◽  
Target Gene ◽  
Target Genes ◽  
Prediction Method ◽  
Peroxisome Proliferator ◽  
Physiological Processes ◽  
Peroxisome Proliferator Activated Receptors ◽  
High Throughput Gene Expression ◽  
Responsive Element ◽  
Target Gene Transcription

The peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors of the nuclear receptor superfamily. Upon ligand binding, PPARs activate target gene transcription and regulate a variety of important physiological processes such as lipid metabolism, inflammation, and wound healing. Here, we describe the first database of PPAR target genes, PPARgene. Among the 225 experimentally verified PPAR target genes, 83 are for PPARα, 83 are for PPARβ/δ, and 104 are for PPARγ. Detailed information including tissue types, species, and reference PubMed IDs was also provided. In addition, we developed a machine learning method to predict novel PPAR target genes by integratingin silicoPPAR-responsive element (PPRE) analysis with high throughput gene expression data. Fivefold cross validation showed that the performance of this prediction method was significantly improved compared to thein silicoPPRE analysis method. The prediction tool is also implemented in the PPARgene database.

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