scholarly journals Study of Morphological Features and Determination of the Fatty Acid Composition of the Microalgae Lipid Complex

Biomolecules ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1571
Author(s):  
Vyacheslav Dolganyuk ◽  
Anna Andreeva ◽  
Ekaterina Budenkova ◽  
Stanislav Sukhikh ◽  
Olga Babich ◽  
...  

Microalgae are rich in nutrients and biologically active substances such as proteins, carbohydrates, lipids, vitamins, pigments, phycobiliproteins, among others. The lipid composition of the microalgae Chlorella vulgaris, Arthrospira platensis, and Dunaliella salina was screened for the first time. The proposed method for purifying the lipid complex isolated from microalgae’s biomass involved dissolving the lipid-pigment complex in n-hexane for 4 h and stirring at 500 rpm. We found that the largest number of neutral lipids is contained in the biomass of microalgae Arthrospira platensis, fatty acids, polar lipids (glycerophospholipids), and unsaponifiable substances—in the biomass of microalgae Dunaliella salina, chlorophyll, and other impurities—in the biomass of microalgae Chlorella vulgaris. The developed method of purification of the fatty acid composition of the microalgae lipid complex confirmed the content of fatty acids in microalgae, which are of interest for practical use in the production of biologically active components. We also determined the potential of its use in the development of affordable technology for processing microalgae into valuable food and feed additives.

Author(s):  
Т.В. ПЕЛИПЕНКО ◽  
С.К. МУСТАФАЕВ ◽  
М.Р. БРУЯКА

Разработана композиция из натуральных масел из семян льна и расторопши пятнистой. Жирнокислотный состав смеси масел обеспечивает нормальное функционирование и регенерацию кожи и достаточно стабилен к окислению. Масла из семян льна высоколиноленового типа и расторопши пятнистой получены методом холодного отжима на лабораторном прессе FarmetDUO PF3. Выполнены расчеты, определяющие концентрацию исходных масел в смеси с заданным составом жирных кислот, и составлены образцы смеси масел: образец 1 при соотношении 6 : 3 жирных кислот 1 : 1 содержал 33 масла расторопши пятнистой и 67 льняного масла образец 2 при соотношении 6 : 3 жирных кислот 4 : 1 соответственно содержал 27 льняного и 73 масла расторопши. Установлено, что фактическое соотношение жирных кислот 6 : 3 в образце 1 соответственно составило 0,97 : 1, в образце 2 3,8 : 1, что близко к оптимальному. Доля олеиновой кислоты (9) в смеси масел, повышающей их устойчивость к окислению, составила 25 от общего количества жирных кислот. Образцы смеси масел характеризуются средней растекаемостью, невысоким поверхностным натяжением и низкой вязкостью. Введение смеси масел из семян льна и расторопши пятнистой с заданным жирнокислотным составом в рецептуры косметических средств позволит получить биологически активные продукты кремы для сухой кожи, а также питательные кремы с высокими сенсорными свойствами. The composition of natural oils of flax seeds and thistle spotty is developed. The fatty acid composition of the oil mix ensures the normal functioning and regeneration of the skin and is sufficiently stable to oxidation. Samples of the oil from the seeds of the flax with high oleic acid and thistle spotty oil is obtained by cold pressing in a laboratory press FarmetDUO PF3. Calculations determining the concentration of the starting oils in a mixture with given of composition of fatty acids were carried. Samples of the mix of oils composed of: sample 1 when the ratio of 6 : 3 fatty acids of 1 : 1 respectively consists of 33 oil thistle spotty and 67 linseed oil sample 2 when the ratio 6 : 3 fatty acids is 4 : 1 respectively consists of 27 linen 73 oil thistle spotty. It was found that the actual ratio of fatty acids 6: 3 in sample 1 was 0,97 : 1 respectively, in sample 2 3,8 : 1, which is close to optimal. The share of oleic acid (9) in the mix of oils, which increases their resistance to oxidation, was 25 of the total amount of fatty acids. Oil mixture samples are characterized by medium spreadability, low surface tension and low viscosity. The introduction of a mix of flax seed oil and thistle spotty oil with a given fatty acid composition in the formulation of cosmetics will allow to obtain biologically active products creams for dry skin, as well as nourishing creams with high sensory properties.


Author(s):  
Tülay Çimrin ◽  
Murat Demirel

This experiment was carried out to investigate the effects of variant doses of rosemary (Rosmarinus officinalis L.) essential oils (REO), vitamin E (α‐tocopheryl acetate) and antibiotic (chlortetracyclin) on the concentration of vitamin E, fatty acid composition, cholesterol levels in the yolk. In this experiment, 32 weeks old Bovans genotype and 240 white laying hens were randomly separated to six treatment groups with five replicas. While the control group was fed with basal diet the treatment groups were supplemented with 500 mg/kg antibiotics; 200 mg/kg vitamin E; 100, 200, and 300 mg/kg REO to basal diet for 90 consecutive days. The results showed that addition of antibiotics, vitamin E and REO to the diet had no effects on egg cholesterol, while fatty acid composition and vitamin E contents of the yolk were significantly affected. Addition of 100 mg/kg REO to the diet increased total mono unsaturated fatty acids (MUFA) by increasing oleic acid level but decreased Polyunsaturated fatty acids (PUFA) by decreasing linoleic acid content of the yolk. Addition of 200 mg/kg vitamin E to the diet significantly increased vitamin E content of egg. The study showed that the changes in yolk fatty acid composition and vitamin E content due to the feed additives, could affect positively in chemical composition of eggs. In conclusion, 100 mg/kg REO and 200 mg/kg vitamin E supplementation to the diet might cause high antioxidant capacity and could help to produce eggs that were more resistant to lipid oxidation.


Author(s):  
N. L. Kleymenova

Milk thistle seeds are a unique source of biologically active substances. The oilseed crop studied is used in various fields, both for food purposes, animal husbandry and in medicine. The characteristics of milk thistle oil were considered in the work. Milk thistle seeds are known to contain 35% vegetable oil (seed fat). The oil was obtained with cold pressing. Milk thistle seed oil was chosen as the object of study. Using an experimental extruder, the oil was obtained under the following modes: the annular gap of the grain chamber - 1.25 mm, the screw rotation speed - 190 rpm, the pressing temperature - 353 K. A comparative study of the fatty acid composition of milk thistle oil components with literary sources was carried out. Gas-liquid chromatography on a Chromotek 5000 device was used to determine the composition of the components according to the GOST 31665-2012 method. The calculation for the components of milk thistle oil  on the basis of which the chromatograms were obtained for the amount of fatty acids was made. It was found out that the characteristics of the test sample are comparable with the literature data, but they also have differences, since other fatty acids were found in the oil. 24 fatty acids were identified by gas chromatographic analysis. The following ratio of fatty acids was determined: linoleic - 53%, oleic - 26%, palmitic - 8%, stearic (5%), arachidic (3%) and behenic (2%). The sample under study contains saturated and unsaturated fatty acids. According to the results of the vitamin composition analysis  milk thistle oil contains vitamins A, E and K and minor β-carotene traces which are a valuable source for diet and preventive nutrition.


1985 ◽  
Vol 54 (03) ◽  
pp. 563-569 ◽  
Author(s):  
M K Salo ◽  
E Vartiainen ◽  
P Puska ◽  
T Nikkari

SummaryPlatelet aggregation and its relation to fatty acid composition of platelets, plasma and adipose tissue was determined in 196 randomly selected, free-living, 40-49-year-old men in two regions of Finland (east and southwest) with a nearly twofold difference in the IHD rate.There were no significant east-southwest differences in platelet aggregation induced with ADP, thrombin or epinephrine. ADP-induced platelet secondary aggregation showed significant negative associations with all C20-C22 ω3-fatty acids in platelets (r = -0.26 - -0.40) and with the platelet 20: 5ω3/20: 4ω 6 and ω3/ ω6 ratios, but significant positive correlations with the contents of 18:2 in adipose tissue (r = 0.20) and plasma triglycerides (TG) (r = 0.29). Epinephrine-induced aggregation correlated negatively with 20: 5ω 3 in plasma cholesteryl esters (CE) (r = -0.23) and TG (r = -0.29), and positively with the total percentage of saturated fatty acids in platelets (r = 0.33), but had no significant correlations with any of the ω6-fatty acids. Thrombin-induced aggregation correlated negatively with the ω3/6ω ratio in adipose tissue (r = -0.25) and the 20: 3ω6/20: 4ω 6 ratio in plasma CE (r = -0.27) and free fatty acids (FFA) (r = -0.23), and positively with adipose tissue 18:2 (r = 0.23) and 20:4ω6 (r = 0.22) in plasma phospholipids (PL).The percentages of prostanoid precursors in platelet lipids, i. e. 20: 3ω 6, 20: 4ω 6 and 20 :5ω 3, correlated best with the same fatty acids in plasma CE (r = 0.32 - 0.77) and PL (r = 0.28 - 0.74). Platelet 20: 5ω 3 had highly significant negative correlations with the percentage of 18:2 in adipose tissue and all plasma lipid fractions (r = -0.35 - -0.44).These results suggest that, among a free-living population, relatively small changes in the fatty acid composition of plasma and platelets may be reflected in significant differences in platelet aggregation, and that an increase in linoleate-rich vegetable fat in the diet may not affect platelet function favourably unless it is accompanied by an adequate supply of ω3 fatty acids.


Author(s):  
Mohamed Haniff Hanafy Idris ◽  
Yanty Noorzianna Manaf ◽  
Mohd Nasir Mohd Desa ◽  
Amalia Mohd Hashim ◽  
Muhamad Shirwan Abdullah Sani ◽  
...  

Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 941
Author(s):  
Ewa Szpunar-Krok ◽  
Anna Wondołowska-Grabowska ◽  
Dorota Bobrecka-Jamro ◽  
Marta Jańczak-Pieniążek ◽  
Andrzej Kotecki ◽  
...  

Soybean is a valuable protein and oilseed crop ranked among the most significant of the major crops. Field experiments were carried out in 2016–2019 in South-East Poland. The influence of soybean cultivars (Aldana, Annushka), nitrogen fertilizer (0, 30, 60 kg∙ha−1 N) and inoculation with B. japonicum (control, HiStick® Soy, Nitragina) on the content of fatty acids (FA) in soybean seeds was investigated in a three-factorial experiment. This study confirms the genetic determinants of fatty acid composition in soybean seeds and their differential accumulation levels for C16:0, C16:1, C18:1n9, C18:2, C18:3, and C20:0 as well saturated (SFA), monounsaturated (MUFA), and polyunsaturated (PUFA) fatty acids. Increasing the rate from 30 to 60 kg ha−1 N did not produce the expected changes, suggesting the use of only a “starter” rate of 30 kg ha−1 N. Inoculation of soybean seeds with a strain of Bradyrhizobium japonicum (HiStick® Soy, BASF, Littlehampton, UK and Nitragina, Institute of Soil Science and Plant Cultivation–State Research Institute, Puławy, Poland) is recommended as it will cause a decrease in SFA and C16:0 acid levels. This is considered nutritionally beneficial as its contribution to total fatty acids determines the hypercholesterolemic index, and it is the third most accumulated fatty acid in soybean seeds. The interaction of cultivars and inoculation formulation on fatty acid content of soybean seeds was demonstrated. An increase in the value of C16:0 content resulted in a decrease in the accumulation of C18:1, C18:2, and C18:3 acids. The content of each decreased by almost one unit for every 1% increase in C16:0 content. The dominant effect of weather conditions on the FA profile and C18:2n6/C18:3n3 ratio was demonstrated. This suggests a need for further evaluation of the genetic progress of soybean cultivars with respect to fatty acid composition and content under varying habitat conditions.


Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1633
Author(s):  
Michal Rolinec ◽  
Daniel Bíro ◽  
Milan Šimko ◽  
Miroslav Juráček ◽  
Ondrej Hanušovský ◽  
...  

The utilisation of different by-products from the food industry as nutrient sources for farm animals is both possible and beneficial. Grape pomace is a by-product that contains polyphenols and fatty acids, both of which have the potential to improve the nutritional quality of cow colostrum. This study aimed to explore how the addition of grape pomace to the diet of dry cows affects the concentration of nutrients and fatty acids of colostrum. Sixteen Slovak spotted cows in late pregnancy were used in this study. From the seventh day before expected calving to the day of calving, cows in the grape pomace group received a diet supplemented with dried grape pomace, at 0.116 kg/cow/day. Colostrum samples were analysed for basal nutrients and fatty acid concentrations. Between the control and experimental groups, the nutrient and fatty acid concentrations of all the colostrum samples did not show significant differences. The nutrient levels in the colostrum from both groups of cows were typical, as related to the time from calving. The addition of the grape pomace into the diet of dry cows had no effect on nutrient concentrations and the fatty acid composition of the colostrum. The somatic cell score of the colostrum sampled at the 12th hour after calving (4.2 versus 2.6) was positively affected by grape pomace addition. The results of this study revealed that grape pomace (fed in an amount of 0.116 kg/cow/day) had no positive or negative effect on the base nutrients and fatty acids of cow colostrum, and, therefore, grape pomace can be used as a nutrient source for dry cows in small amounts.


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