scholarly journals Development of Cellular and Enzymatic Bioluminescent Assay Systems to Study Low-Dose Effects of Thorium

2021 ◽  
Vol 8 (12) ◽  
pp. 194
Author(s):  
Olga V. Kolesnik ◽  
Tatiana V. Rozhko ◽  
Maria A. Lapina ◽  
Vladislav S. Solovyev ◽  
Anna S. Sachkova ◽  
...  
Keyword(s):  
Low Dose ◽  
Quantitative Measure ◽  
Active Role ◽  
Photobacterium Phosphoreum ◽  
Enzymatic Reactions ◽  
Natural Ecosystems ◽  
Bacterial Bioluminescence ◽  
Luminescent Bacteria ◽  
Living Organisms

Thorium is one of the most widespread radioactive elements in natural ecosystems, along with uranium, it is the most important source of nuclear energy. However, the effects of thorium on living organisms have not been thoroughly studied. Marine luminescent bacteria and their enzymes are optimal bioassays for studying low-dose thorium exposures. Luminescent bioassays provide a quantitative measure of toxicity and are characterized by high rates, sensitivity, and simplicity. It is known that the metabolic activity of bacteria is associated with the production of reactive oxygen species (ROS). We studied the effects of thorium-232 (10−11–10−3 M) on Photobacterium phosphoreum and bacterial enzymatic reactions; kinetics of bacterial bioluminescence and ROS content were investigated in both systems. Bioluminescence activation was revealed under low-dose exposures (<0.1 Gy) and discussed in terms of “radiation hormesis”. The activation was accompanied by an intensification of the oxidation of a low-molecular reducer, NADH, during the enzymatic processes. Negative correlations were found between the intensity of bioluminescence and the content of ROS in bacteria and enzyme systems; an active role of ROS in the low-dose activation by thorium was discussed. The results contribute to radioecological potential of bioluminescence techniques adapted to study low-intensity radioactive exposures.

The role of the structure to provide biogeocenotic soil functions

2016 ◽  
Vol 27 (3-4) ◽  
pp. 89-96
Author(s):  
V. A. Gorban
Keyword(s):  
Organic Matter ◽  
Structural State ◽  
Soil Conditions ◽  
Biological Processes ◽  
Ecological Community ◽  
Soil Functions ◽  
Natural Ecosystems ◽  
Physical Chemical ◽  
Living Organisms

There is a presentation of analysis of the structural state role in the manifestation of biogeocenotic soil functions, which are due to their physical, chemical and biochemical, physical-chemical, information, integrity properties. The main role in the formation of the structure of living space manifests itself in existence, due to the presence in soil aggregates of different sizes and structures, of numerous and diverse ecological niches for soil organisms. The participation of structure in providing housing and shelter is evident in its impact on the majority of soil conditions. The role of the structure in the manifestation of the support is associated with fixing function in plants through the soil root system, as well as with structural features of the surface soil, which is used by animals when moving. The participate of structure in preservation of plant seeds, eggs and larvae of animal is due to the formation of aggregated soil optimal living conditions for living organisms. The structure plays an important role in realization of the soil nutrients and source connections, as plants are able to obtain only minerals in dissolved form and the optimal conditions for this can provide only soil with qualitative waterproof structure. The structure also participates in the formation of a depot of nutrients, energy and moisture, most of which are found in soil and aggregates that can be made available to living organisms after their destruction. The role of the of structure in providing stimulator function and inhibitor of biochemical processes is evident in the formation of a kind of the medium through which the interaction of the root systems of plants, as well as providing conditions for humification and mineralization of soil organic matter. The influence of the structure on the sorption of particulate material coming from the atmosphere is evident in its involvement in the formation of structural units of the soil. The role of the structure in the sorption of fine soil microorganisms living in the soil is due to the fact that this process is largely determined by the shape of aggregates, mineral composition and organic matter, which are included in their composition. The features of the structure to provide a signal function to the seasonal biological processes are related to the determining the influence of the aggregate composition of the soil at its thermal regime, which mainly depends on all biological processes in the soil. The role of the structure in the regulation of number and composition of the ecological community is due to the existence of the relationship between aggregate soil composition and characteristics of ecological community that exists on it. Participation in the launch of some of the structures of succession occurs mainly at the sharp deterioration of the structural state of the soil, which may be associated with the processes of water and wind erosion. The role of the structure to provide a «memory» of biogeocoenosis is explained by quite stable structural state of the natural ecosystems of the soil in the absence of catastrophic processes, which is able to store information about the features of the state of other components of biogeocoenosis. Participation of the structure in the transformation of matter and energy entering biogeocoenosis is due to the constant transformation of minerals, grain size elements, organic matter and other constituent parts of the soil in the process of aggregation. The role of the structure in the display of the sanitary function is due to the fact that there is a degradation of organic residues and metabolic products of living organisms in the soil, its antiseptic properties is best evident only in well-structured soil. The value of the structure in the function of the protective screen and buffer biogeocenotic screen provision is most fully shown in the soils of natural ecosystems with a favorable and stable structural state, due to which ecosystems are protected from mechanical destruction under the influence of various factors – water, wind, gravity force. Despite the cited aspects of the role of structure manifestation in providing biogeocenotic soil functions there is a need for further detailed research in this area.

Role of spectrin in membrane fusion and in shape change of human erythrocyte ghost

1978 ◽  
Vol 36 (2) ◽  
pp. 328-329
Author(s):  
Hideo Hayashi ◽  
Yoshikazu Hirai ◽  
John T. Penniston
Keyword(s):  
Conformational Changes ◽  
Human Erythrocyte ◽  
Shape Change ◽  
Membrane Surface ◽  
Slight Modification ◽  
Active Role ◽  
Main Role ◽  
Bank Blood ◽  
Human Erythrocyte Ghost

Spectrin is a membrane associated protein most of which properties have been tentatively elucidated. A main role of the protein has been assumed to give a supporting structure to inside of the membrane. As reported previously, however, the isolated spectrin molecule underwent self assemble to form such as fibrous, meshwork, dispersed or aggregated arrangements depending upon the buffer suspended and was suggested to play an active role in the membrane conformational changes. In this study, the role of spectrin and actin was examined in terms of the molecular arrangements on the erythrocyte membrane surface with correlation to the functional states of the ghosts.Human erythrocyte ghosts were prepared from either freshly drawn or stocked bank blood by the method of Dodge et al with a slight modification as described before. Anti-spectrin antibody was raised against rabbit by injection of purified spectrin and partially purified.

Elucidation of block boundaries as the dissolution channels in hydrated cement

1978 ◽  
Vol 36 (1) ◽  
pp. 484-485
Author(s):  
N.V. Belov ◽  
U.I. Papiashwili ◽  
B.E. Yudovich
Keyword(s):  
Liquid Phase ◽  
Grain Boundaries ◽  
Benzoyl Peroxide ◽  
Phase Contrast ◽  
Active Role ◽  
Point Of View ◽  
Hardened Cement ◽  
Hydrated Cement ◽  
Hardened Cement Paste

It has been almost universally adopted that dissolution of solids proceeds with development of uniform, continuous frontiers of reaction.However this point of view is doubtful / 1 /. E.g. we have proved the active role of the block (grain) boundaries in the main phases of cement, these boundaries being the areas of hydrate phases' nucleation / 2 /. It has brought to the supposition that the dissolution frontier of cement particles in water is discrete. It seems also probable that the dissolution proceeds through the channels, which serve both for the liquid phase movement and for the drainage of the incongruant solution products. These channels can be appeared along the block boundaries.In order to demonsrate it, we have offered the method of phase-contrast impregnation of the hardened cement paste with the solution of methyl metacrylahe and benzoyl peroxide. The viscosity of this solution is equal to that of water.

Potential Uses of Vinegar as a Medicine and Related in vivo Mechanisms

2016 ◽  
Vol 86 (3-4) ◽  
pp. 127-151 ◽  
Author(s):  
Zeshan Ali ◽  
Zhenbin Wang ◽  
Rai Muhammad Amir ◽  
Shoaib Younas ◽  
Asif Wali ◽  
...  
Keyword(s):  
Chemical Structure ◽  
Review Paper ◽  
Heart Diseases ◽  
Folk Medicine ◽  
Active Role ◽  
Current Review ◽  
Different Types ◽  
Positive Effect

While the use of vinegar to fi ght against infections and other crucial conditions dates back to Hippocrates, recent research has found that vinegar consumption has a positive effect on biomarkers for diabetes, cancer, and heart diseases. Different types of vinegar have been used in the world during different time periods. Vinegar is produced by a fermentation process. Foods with a high content of carbohydrates are a good source of vinegar. Review of the results of different studies performed on vinegar components reveals that the daily use of these components has a healthy impact on the physiological and chemical structure of the human body. During the era of Hippocrates, people used vinegar as a medicine to treat wounds, which means that vinegar is one of the ancient foods used as folk medicine. The purpose of the current review paper is to provide a detailed summary of the outcome of previous studies emphasizing the role of vinegar in treatment of different diseases both in acute and chronic conditions, its in vivo mechanism and the active role of different bacteria.

Russia’s Market of Mergers and Acquisitions: Stages, Features and Prospects

2009 ◽  
pp. 23-45 ◽  
Author(s):  
A. Radygin
Keyword(s):  
Open Access ◽  
Mergers And Acquisitions ◽  
Corporate Control ◽  
Active Role ◽  
Data Bases ◽  
2008 Financial Crisis ◽  
Legal Norms ◽  
Role Of The State ◽  
Access Data

The article deals with key tendencies in the development of Russia’s market of mergers and acquisitions in the first decade of the 21st century. Quantitative parameters are analyzed by using available in the open access data bases for the years 2003-2008 taking into consideration new tendencies relating to 2008 financial crisis. An active role of the state played in the market of corporate control represents an important factor. Special attention is given to issues of development of Russia’s system of legal norms regulating the market of mergers and acquisitions.

The issue of radioactive contamination in context of ecosystem services development

2016 ◽  
Vol 3 (3) ◽  
pp. 48-53 ◽  
Author(s):  
G. Chobotko ◽  
L. Raychuk ◽  
I. McDonald
Keyword(s):  
Ecosystem Services ◽  
The State ◽  
Natural Ecosystems ◽  
Type Region ◽  
State Register ◽  
Agrarian Policy ◽  
Literary Sources ◽  
Radiation Contamination ◽  
Logical Research

The aim of the article was to defi ne the role of the radioactive environment contamination in the formation of ecosystem services strategy. Methods. Monographic, systemic and structural, factor analysis, abstract and logical research methods have been used. The data from the State Statistics Service of Ukraine, the Ministry of Agrarian Policy and Food of Ukraine, the Ministry of Ecology and Natural Resources of Ukraine, materials of scientifi c researches, international materials and reports and other literary sources on the issues investigated have been used as an information base. Results. Retrospective analysis of sources and state of radioactive eco- systems contamination was conducted and the priority steps in developing the concept of ecosystem services in conditions of radiation contamination were found. Conclusions. The current socio-ecological paradigm of the transition from environmental use to environmental management should be refl ected in the relevant envi- ronmental management mechanisms. Currently, when assessing the state of ecosystem services in Ukraine and worldwide one must take into account the changes in food demand of residents of radioactively contaminated areas, the exploitation of radioactively safe ecosystems growth, their overload and degradation. All of this re- quires an inventory of ecosystem services by type, region, consumers, etc. and the formation of a state register of ecosystem services with a clear assignment of area of responsibility for appropriate natural ecosystems. This will help to make the economic evaluation of different ecosystem services and mechanisms of charges for ecosystem services.

Beyond the Social Determinants of Health

2020 ◽  
Vol 3 ◽  
pp. 1-11
Author(s):  
Laura Hall ◽  
Urpi Pine ◽  
Tanya Shute
Keyword(s):  
Labour Force ◽  
Methodological Approach ◽  
Active Role ◽  
Indigenous Women ◽  
Economic Activities ◽  
The Earth ◽  
The Social ◽  
Weather Changes ◽  
People First

Abstract This paper will reflect on key findings from a Summer 2017 initiative entitled The Role of Culture and Land-Based Healing in Addressing and Ending Violence against Indigenous Women and Two-Spirited People. The Indigenist and decolonizing methodological approach of this work ensured that all research was grounded in experiential and reciprocal ways of learning. Two major findings guide the next phase of this research, complicating the premise that traditional economic activities are healing for Indigenous women and Two-Spirit people. First, the complexities of the mainstream labour force were raised numerous times. Traditional economies are pressured in ongoing ways through exploitative labour practices. Secondly, participants emphasized the importance of attending to the responsibility of nurturing, enriching, and sustaining the wellbeing of soil, water, and original seeds in the process of creating renewal gardens as a healing endeavour. In other words, we have an active role to play in healing the environment and not merely using the environment to heal ourselves. Gardening as research and embodied knowledge was stressed by extreme weather changes including hail in June, 2018, which meant that participants spent as much time talking about the healing of the earth and her systems as the healing of Indigenous women in a context of ongoing colonialism.

Integration of Self-diagnosis in Pascal Law Learning Using STEM Approach

2020 ◽  
Vol 12 (2) ◽  
pp. 104
Author(s):  
Siti Hannah Padliyyah
Keyword(s):  
International Student ◽  
Student Assessment ◽  
Active Role ◽  
Stem Learning ◽  
International Student Assessment ◽  
Science Technology ◽  
Engineering Mathematics ◽  
Post Test ◽  
11Th Grade

Indonesia is ranked 56th out of 65 participating countries in the Program for International Student Assessment (PISA) based on data 2015. According to PISA results, the average science score of Indonesian students is 403, where this number is categorized as low. This is because students are still in the process of understanding and have not yet fully recognized the location of their mistakes. Students can diagnose the location of their mistakes through self-diagnosis activities. Self-diagnosis activities require the active role of students during the learning process. One approach that can increase the active role of students is STEM (Science Technology Engineering Mathematics). However, research at this time is still rarely found self-diagnosis activities that are applied to the STEM approach. Therefore, this research has the aim to find out the increase in mastery of physical concepts and self-diagnosis of students on the STEM learning approach to the theory of poscal law class XI High School.This study uses a One-Group pretest-posttest design with a sample of 30 ini 11th grade highschool from one schools in Bandung. . Based on the findings, there is an increase in mastery of concepts [<g> = 0.51] from pre-test to post-test. In self-diagnosis activities identified that there are differences in scores [z = 1.75; p = 0.9599] student assessment results of researchers and self-scoring results. Deeper self-diagnosis triggers a series of implicit steps that encourage them to rearrange their cognition by correcting the mistakes they make when solving problems. So that learning activities using the STEM approach that involves self-diagnosis activities can improve students' mastery of concepts.

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