scholarly journals A Systematic Experimental and Computational Analysis of Commercially Available Aliphatic Polyesters

2019 ◽  
Vol 9 (16) ◽  
pp. 3397
Author(s):  
Tommaso Casalini ◽  
Monica Bassas-Galia ◽  
Hervé Girard ◽  
Andrea Castrovinci ◽  
Alessandro De Carolis ◽  
...  
Keyword(s):  
Computational Analysis ◽  
Degradation Products ◽  
Degradation Kinetics ◽  
Experimental Studies ◽  
Polyglycolic Acid ◽  
Local Drug Delivery ◽  
Aliphatic Polyesters ◽  
Wide Range ◽  
Material Choice ◽  
Optimal Material

Aliphatic polyesters, such as polylactic acid (PLA), polyglycolic acid (PGA), and their copolymer polylactic-co-glycolic acid (PLGA) have become an established choice in the biomedical field in a wide range of applications, from nanoparticles for local drug delivery to bone fixation screws, and, hence, in a huge spectrum of uses in different medical devices currently available on the market worldwide. The reason for their popularity lies in their combination of interesting peculiarities: in situ degradation, intrinsic biocompatibility (degradation products are recognized and metabolized), processability with standard industrial technologies, and tailorable properties. The knowledge of the degradation rate is an essential requirement for optimal device design when, e.g., fast adsorption time is required, or mechanical properties must be assured over a given time span. In this regard, experimental studies can be time- and money-consuming, due to the time scales (weeks–months) involved in the hydrolysis process. This work aims at providing to both industry and academia robust guidelines for optimal material choice through a systematic experimental and computational analysis of most commonly used PLGA formulations (selected from commercially available products), evaluating the degradation kinetics and its impact on polymer properties.

A method for determining the generalized energy grindability index of particulate solids

1970 ◽  
Author(s):  
Vladimir P. Zhukov ◽  
Dmitriy А. Osipov ◽  
Vadim E. Mizonov ◽  
Dariusz Urbaniak
Keyword(s):  
Computational Analysis ◽  
Bulk Material ◽  
Experimental Studies ◽  
Strength Properties ◽  
Grinding Process ◽  
Particle Sizes ◽  
Actual Problem ◽  
Particulate Solids ◽  
Wide Range ◽  
Low Efficiency

To calculate and evaluate the efficiency of the processes of fractionation of particulate solids, various industry methods have been developed that are widely and successfully used for the analysis of specific industrial technologies. Different indicators are used to measure the strength properties of particles of a bulk material in various industrial technologies. The disadvantage of the situation is the complexity, and often the impossibility of using these specific indices of strength of materials for calculating the grinding process in new equipment or a new range of particle sizes. Significant energy consumption for grinding at low efficiency of industrial mills causes the necessity of carrying out the studies aimed to increasing the efficiency of fractionation of particulate solids. In connection with this, the actual problem of scientific research to develop a generalized and universal energy indicator of grindability particulate solids and a method for its determination that can be used to describe the grinding process and to evaluate its efficiency in various industrial technologies for a wide range of materials arises.Calculation studies were carried out within the framework of the thermodynamic approach to model a grinding process. In order to carry out experimental studies, a special program for measuring and processing experimental data obtained at the laboratory impact mill was developed.In the course of computational and experimental studies, a technique has been proposed to determine the energy grindability index of particulate solids. The relation of this index with the energy necessary for breakdown of the intermolecular bonds in the material under study is established.The carried out researches allowed substantiating the method of determining the generalized energy grindability index of particulate solids. Computational analysis showed that the technique with acceptable accuracy for engineering calculations can be used in practice to assess the possibility of enrichment of heterogeneous components and to determine the optimal technological conditions for the separation of these components.

Validated Stability Indicating HPTLC Method for the Estimation of Amoxapine in Different Stress Conditions

2019 ◽  
Vol 15 (3) ◽  
pp. 273-279
Author(s):  
Shweta G. Rangari ◽  
Nishikant A. Raut ◽  
Pradip W. Dhore
Keyword(s):  
High Performance ◽  
Limit Of Detection ◽  
Degradation Products ◽  
Analysis Data ◽  
Peak Height ◽  
Good Resolution ◽  
Stability Indicating ◽  
Limit Of Quantitation ◽  
Wide Range ◽  
Hptlc Method

Background:The unstable and/or toxic degradation products may form due to degradation of drug which results into loss of therapeutic activity and lead to life threatening condition. Hence, it is important to establish the stability characteristics of drug in various conditions such as in temperature, light, oxidising agent and susceptibility across a wide range of pH values.Introduction:The aim of the proposed study was to develop simple, sensitive and economic stability indicating high performance thin layer chromatography (HPTLC) method for the quantification of Amoxapine in the presence of degradation products.Methods:Amoxapine and its degraded products were separated on precoated silica gel 60F254 TLC plates by using mobile phase comprising of methanol: toluene: ammonium acetate (6:3:1, v/v/v). The densitometric evaluation was carried out at 320 nm in reflectance/absorbance mode. The degradation products obtained as per ICH guidelines under acidic, basic and oxidative conditions have different Rf values 0.12, 0.26 and 0.6 indicating good resolution from each other and pure drug with Rf: 0.47. Amoxapine was found to be stable under neutral, thermal and photo conditions.Results:The method was validated as per ICH Q2 (R1) guidelines in terms of accuracy, precision, ruggedness, robustness and linearity. A good linear relationship between concentration and response (peak area and peak height) over the range of 80 ng/spot to 720 ng/spot was observed from regression analysis data showing correlation coefficient 0.991 and 0.994 for area and height, respectively. The limit of detection (LOD) and limit of quantitation (LOQ) for area were found to be 1.176 ng/mL and 3.565 ng/mL, whereas for height, 50.063 ng/mL and 151.707 ng/mL respectively.Conclusion:The statistical analysis confirmed the accuracy, precision and selectivity of the proposed method which can be effectively used for the analysis of amoxapine in the presence of degradation products.

Multi-chain action of exo-D-galacturonanase from carrot

1983 ◽  
Vol 48 (12) ◽  
pp. 3579-3588
Author(s):  
Kveta Heinrichová ◽  
Jana Perečková
Keyword(s):  
Optimal Condition ◽  
Degradation Products ◽  
Degradation Kinetics ◽  
Hydrolytic Cleavage ◽  
Chain Mechanism ◽  
Modes Of Action ◽  
Enzyme Degradation ◽  
Good Agreement

Two possible modes of action of exo-D-galacturonanase from carrot (E.C. 3.2.1.67) were investigated; this enzyme catalyses the sequential hydrolytic cleavage of pectants and oligogalacturonans by a terminal action from the nonreducing end of the molecule. The experiments indicate that the investigated exo-D-galacturonanase degrades these substrates by a predominantly multi-chain mechanism. Distribution of degradation products of oligomeric substrates (hexa- and pentagalacturonide) under an optimal condition for the action of the enzyme (pH and temperature) indicates that a multi-chain enzyme attack with a prevalent simple collision is involved. Results of the enzyme degradation kinetics are in a good agreement with the above-mentioned presumption.

scholarly journals Degradation Products of Polychlorinated Biphenyls and Their In Vitro Transformation by Ligninolytic Fungi

Toxics ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 81
Author(s):  
Kamila Šrédlová ◽  
Kateřina Šírová ◽  
Tatiana Stella ◽  
Tomáš Cajthaml
Keyword(s):  
Polychlorinated Biphenyls ◽  
Extracellular Enzymes ◽  
Degradation Products ◽  
Transformation Products ◽  
Irpex Lacteus ◽  
Chlorobenzoic Acid ◽  
Ligninolytic Fungi ◽  
Wide Range ◽  
Pcb Metabolites

Metabolites of polychlorinated biphenyls (PCBs)—hydroxylated PCBs (OH‑PCBs), chlorobenzyl alcohols (CB‑OHs), and chlorobenzaldehydes (CB‑CHOs)—were incubated in vitro with the extracellular liquid of Pleurotus ostreatus, which contains mainly laccase and low manganese-dependent peroxidase (MnP) activity. The enzymes were able to decrease the amount of most of the tested OH‑PCBs by > 80% within 1 h; the removal of more recalcitrant OH‑PCBs was greatly enhanced by the addition of the laccase mediator syringaldehyde. Conversely, glutathione substantially hindered the reaction, suggesting that it acted as a laccase inhibitor. Hydroxylated dibenzofuran and chlorobenzoic acid were identified as transformation products of OH‑PCBs. The extracellular enzymes also oxidized the CB‑OHs to the corresponding CB‑CHOs on the order of hours to days; however, the mediated and nonmediated setups exhibited only slight differences, and the participating enzymes could not be determined. When CB‑CHOs were used as the substrates, only partial transformation was observed. In an additional experiment, the extracellular liquid of Irpex lacteus, which contains predominantly MnP, was able to efficiently transform CB‑CHOs with the aid of glutathione; mono‑ and di-chloroacetophenones were detected as transformation products. These results demonstrate that extracellular enzymes of ligninolytic fungi can act on a wide range of PCB metabolites, emphasizing their potential for bioremediation.

scholarly journals Image Statistics Preserving Encrypt-then-Compress Scheme Dedicated for JPEG Compression Standard

Entropy ◽  
2021 ◽  
Vol 23 (4) ◽  
pp. 421
Author(s):  
Dariusz Puchala ◽  
Kamil Stokfiszewski ◽  
Mykhaylo Yatsymirskyy
Keyword(s):  
Statistical Analysis ◽  
Image Encryption ◽  
Experimental Studies ◽  
Quality Measures ◽  
Input Image ◽  
Jpeg Compression ◽  
Image Statistics ◽  
Compression Stage ◽  
Wide Range ◽  
The Impact

In this paper, the authors analyze in more details an image encryption scheme, proposed by the authors in their earlier work, which preserves input image statistics and can be used in connection with the JPEG compression standard. The image encryption process takes advantage of fast linear transforms parametrized with private keys and is carried out prior to the compression stage in a way that does not alter those statistical characteristics of the input image that are crucial from the point of view of the subsequent compression. This feature makes the encryption process transparent to the compression stage and enables the JPEG algorithm to maintain its full compression capabilities even though it operates on the encrypted image data. The main advantage of the considered approach is the fact that the JPEG algorithm can be used without any modifications as a part of the encrypt-then-compress image processing framework. The paper includes a detailed mathematical model of the examined scheme allowing for theoretical analysis of the impact of the image encryption step on the effectiveness of the compression process. The combinatorial and statistical analysis of the encryption process is also included and it allows to evaluate its cryptographic strength. In addition, the paper considers several practical use-case scenarios with different characteristics of the compression and encryption stages. The final part of the paper contains the additional results of the experimental studies regarding general effectiveness of the presented scheme. The results show that for a wide range of compression ratios the considered scheme performs comparably to the JPEG algorithm alone, that is, without the encryption stage, in terms of the quality measures of reconstructed images. Moreover, the results of statistical analysis as well as those obtained with generally approved quality measures of image cryptographic systems, prove high strength and efficiency of the scheme’s encryption stage.

scholarly journals The in vitro assessment of the toxicity of volatile, oxidisable, redox-cycling compounds: phenols as an example

2021 ◽  
Author(s):  
Laia Tolosa ◽  
Teresa Martínez-Sena ◽  
Johannes P. Schimming ◽  
Erika Moro ◽  
Sylvia E. Escher ◽  
...  
Keyword(s):  
Cultured Cells ◽  
Degradation Products ◽  
Degradation Kinetics ◽  
Redox Cycling ◽  
In Vitro Toxicity ◽  
Cross Contamination ◽  
Physicochemical Changes ◽  
In Vitro Assessment ◽  
Biological Performance

AbstractPhenols are regarded as highly toxic chemicals. Their effects are difficult to study in in vitro systems because of their ambiguous fate (degradation, auto-oxidation and volatility). In the course of in vitro studies of a series of redox-cycling phenols, we found evidences of cross-contamination in several in vitro high-throughput test systems, in particular by trimethylbenzene-1, 4-diol/trimethylhydroquinone (TMHQ) and 2,6-di-tertbutyl-4-ethylphenol (DTBEP), and investigated in detail the physicochemical basis for such phenomenon and how to prevent it. TMHQ has fast degradation kinetics followed by significant diffusion rates of the resulting quinone to adjacent wells, other degradation products being able to air-diffuse as well. DTBEP showed lower degradation kinetics, but a higher diffusion rate. In both cases the in vitro toxicity was underestimated because of a decrease in concentration, in addition to cross-contamination to neighbouring wells. We identified four degradation products for TMHQ and five for DTBEP indicating that the current effects measured on cells are not only attributable to the parent phenolic compound. To overcome these drawbacks, we investigated in detail the physicochemical changes occurring in the course of the incubation and made use of gas-permeable and non-permeable plastic seals to prevent it. Diffusion was greatly prevented by the use of both plastic seals, as revealed by GC–MS analysis. Gas non-permeable plastic seals, reduced to a minimum compounds diffusion as well oxidation and did not affect the biological performance of cultured cells. Hence, no toxicological cross-contamination was observed in neighbouring wells, thus allowing a more reliable in vitro assessment of phenol-induced toxicity.

scholarly journals Design of 3D Additively Manufactured Hybrid Structures for Cranioplasty

Materials ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 181
Author(s):  
Roberto De Santis ◽  
Teresa Russo ◽  
Julietta V. Rau ◽  
Ida Papallo ◽  
Massimo Martorelli ◽  
...  
Keyword(s):  
Physical Models ◽  
Cranial Bone ◽  
Head Model ◽  
Rigid Sphere ◽  
Element Analysis ◽  
Aliphatic Polyesters ◽  
Hybrid Device ◽  
Wide Range ◽  
Hybrid Devices ◽  
Technical Solutions

A wide range of materials has been considered to repair cranial defects. In the field of cranioplasty, poly(methyl methacrylate) (PMMA)-based bone cements and modifications through the inclusion of copper doped tricalcium phosphate (Cu-TCP) particles have been already investigated. On the other hand, aliphatic polyesters such as poly(ε-caprolactone) (PCL) and polylactic acid (PLA) have been frequently investigated to make scaffolds for cranial bone regeneration. Accordingly, the aim of the current research was to design and fabricate customized hybrid devices for the repair of large cranial defects integrating the reverse engineering approach with additive manufacturing, The hybrid device consisted of a 3D additive manufactured polyester porous structures infiltrated with PMMA/Cu-TCP (97.5/2.5 w/w) bone cement. Temperature profiles were first evaluated for 3D hybrid devices (PCL/PMMA, PLA/PMMA, PCL/PMMA/Cu-TCP and PLA/PMMA/Cu-TCP). Peak temperatures recorded for hybrid PCL/PMMA and PCL/PMMA/Cu-TCP were significantly lower than those found for the PLA-based ones. Virtual and physical models of customized devices for large cranial defect were developed to assess the feasibility of the proposed technical solutions. A theoretical analysis was preliminarily performed on the entire head model trying to simulate severe impact conditions for people with the customized hybrid device (PCL/PMMA/Cu-TCP) (i.e., a rigid sphere impacting the implant region of the head). Results from finite element analysis (FEA) provided information on the different components of the model.

scholarly journals Effects of electrical stimulation on isolated rodent gastric smooth muscle cells evaluated via a joint computational simulation and experimental approach

2009 ◽  
Vol 297 (4) ◽  
pp. G672-G680 ◽  
Author(s):  
P. Du ◽  
S. Li ◽  
G. O'Grady ◽  
L. K. Cheng ◽  
A. J. Pullan ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Electrical Stimulation ◽  
Pulse Train ◽  
Pulse Width ◽  
Computational Simulation ◽  
Minimum Energy ◽  
Experimental Studies ◽  
Single Pulse ◽  
Plateau Phase ◽  
Wide Range

Gastric electrical stimulation (GES) involves the delivery of electrical impulses to the stomach for therapeutic purposes. New GES protocols are needed that are optimized for improved motility outcomes and energy efficiency. In this study, a biophysically based smooth muscle cell (SMC) model was modified on the basis of experimental data and employed in conjunction with experimental studies to define the effects of a large range of GES protocols on individual SMCs. For the validation studies, rat gastric SMCs were isolated and subjected to patch-clamp analysis during stimulation. Experimental results were in satisfactory agreement with simulation results. The results define the effects of a wide range of GES parameters (pulse width, amplitude, and pulse-train frequency) on isolated SMCs. The minimum pulse width required to invoke a supramechanical threshold response from SMCs (defined at −30 mV) was 65 ms (at 250-pA amplitude). The minimum amplitude required to invoke this threshold was 75 pA (at 1,000-ms pulse width). The amplitude of the invoked response beyond this threshold was proportional to the stimulation amplitude. A high-frequency train of stimuli (40 Hz; 10 ms, 150 pA) could invoke and maintain the SMC plateau phase while requiring 60% less power and accruing ∼30% less intracellular Ca2+ concentration during the plateau phase than a comparable single-pulse protocol could in a demonstrated example. Validated computational simulations are an effective strategy for efficiently identifying effective minimum-energy GES protocols, and pulse-train protocols may also help to reduce the power consumption of future GES devices.

Experimental Studies on Cooling Effectiveness of the Double-Decker Air Jet Impingement With Film Outflow

2007 ◽  
Author(s):  
Junkui Mao ◽  
Wen Guo ◽  
Zhenxiong Liu ◽  
Jun Zeng
Keyword(s):  
Infrared Radiation ◽  
Experimental Studies ◽  
Jet Impingement ◽  
Experimental Results ◽  
Thermal Camera ◽  
Local Cooling ◽  
Cooling Effectiveness ◽  
Air Jet ◽  
Double Decker ◽  
Wide Range

Experiments were carried out to investigate the cooling effectiveness of a lamellar double-decker impingement/effusion structure. Infrared radiation (I.R.) thermal camera was used to measure the temperature on the outside surface of the lamellar double-decker. Experimental results were obtained for a wide range of governing parameters (blowing rate M (0.0017∼0.0066), the ratio of the jet impingement distance to the diameter of film hole H/D (0.5∼1.25), the ratio of the distance between the jet hole and film hole to the diameter of the film hole P/D (0, 3, 4), and the material of double-decker (Steel and Copper)). It was observed that the local cooling effectiveness η varies with all these parameters in a complicated way. All the results show that higher cooling effectiveness η is achieved in larger blowing rate cases. A certain range of H/D and P/D can be designed to result in the maximum cooling effectiveness η. And η is less sensitive to the material type compared with those parameters such as H/D, M and P/D.

Experimental Studies of Leading Edge Contouring Influence on Secondary Losses in Transonic Turbines

2012 ◽  
Author(s):  
Ranjan Saha ◽  
Jens Fridh ◽  
Torsten Fransson ◽  
Boris I. Mamaev ◽  
Mats Annerfeldt
Keyword(s):  
Gas Turbine ◽  
Guide Vane ◽  
Experimental Studies ◽  
Three Dimensional ◽  
Leading Edge ◽  
Secondary Flows ◽  
Noticeable Effect ◽  
Flow Angle ◽  
Wide Range ◽  
Contour Plots

An experimental study of the hub leading edge contouring using fillets is performed in an annular sector cascade to observe the influence of secondary flows and aerodynamic losses. The investigated vane is a three dimensional gas turbine guide vane (geometrically similar) with a mid-span aspect ratio of 0.46. The measurements are carried out on the leading edge fillet and baseline cases using pneumatic probes. Significant precautions have been taken to increase the accuracy of the measurements. The investigations are performed for a wide range of operating exit Mach numbers from 0.5 to 0.9 at a design inlet flow angle of 90°. Data presented include the loading, fields of total pressures, exit flow angles, radial flow angles, as well as profile and secondary losses. The vane has a small profile loss of approximately 2.5% and secondary loss of about 1.1%. Contour plots of vorticity distributions and velocity vectors indicate there is a small influence of the vortex-structure in endwall regions when the leading edge fillet is used. Compared to the baseline case the loss for the filleted case is lower up to 13% of span and higher from 13% to 20% of the span for a reference condition with Mach no. of 0.9. For the filleted case, there is a small increase of turning up to 15% of the span and then a small decrease up to 35% of the span. Hence, there are no significant influences on the losses and turning for the filleted case. Results lead to the conclusion that one cannot expect a noticeable effect of leading edge contouring on the aerodynamic efficiency for the investigated 1st stage vane of a modern gas turbine.

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