scholarly journals Use of Anionic Polysaccharides in the Development of 3D Bioprinting Technology

2019 ◽  
Vol 9 (13) ◽  
pp. 2596 ◽  
Author(s):  
Chia Tai ◽  
Soukaina Bouissil ◽  
Enkhtuul Gantumur ◽  
Mary Stephanie Carranza ◽  
Ayano Yoshii ◽  
...  
Keyword(s):  
Biomedical Applications ◽  
Three Dimensional ◽  
Cell Generation ◽  
Present Review ◽  
3D Bioprinting ◽  
Significant Progress ◽  
Anionic Polysaccharides ◽  
Reactive Groups ◽  
The Way

Three-dimensional (3D) bioprinting technology is now one of the best ways to generate new biomaterial for potential biomedical applications. Significant progress in this field since two decades ago has pointed the way toward use of natural biopolymers such as polysaccharides. Generally, these biopolymers such as alginate possess specific reactive groups such as carboxylate able to be chemically or enzymatically functionalized to generate very interesting hydrogel structures with biomedical applications in cell generation. This present review gives an overview of the main natural anionic polysaccharides and focuses on the description of the 3D bioprinting concept with the recent development of bioprinting processes using alginate as polysaccharide.

scholarly journals The Adoption of Three-Dimensional Additive Manufacturing from Biomedical Material Design to 3D Organ Printing

2019 ◽  
Vol 9 (4) ◽  
pp. 811 ◽  
Author(s):  
Ajay Vikram Singh ◽  
Mohammad Hasan Dad Ansari ◽  
Shuo Wang ◽  
Peter Laux ◽  
Andreas Luch ◽  
...  
Keyword(s):  
Organ Transplant ◽  
Three Dimensional ◽  
Material Design ◽  
3D Bioprinting ◽  
Biomedical Material ◽  
Recent Developments ◽  
Physicochemical Basis ◽  
Organ Printing ◽  
3D Spheroids ◽  
The Way

Three-dimensional (3D) bioprinting promises to change future lifestyle and the way we think about aging, the field of medicine, and the way clinicians treat ailing patients. In this brief review, we attempt to give a glimpse into how recent developments in 3D bioprinting are going to impact vast research ranging from complex and functional organ transplant to future toxicology studies and printed organ-like 3D spheroids. The techniques were successfully applied to reconstructed complex 3D functional tissue for implantation, application-based high-throughput (HTP) platforms for absorption, distribution, metabolism, and excretion (ADME) profiling to understand the cellular basis of toxicity. We also provide an overview of merits/demerits of various bioprinting techniques and the physicochemical basis of bioink for tissue engineering. We briefly discuss the importance of universal bioink technology, and of time as the fourth dimension. Some examples of bioprinted tissue are shown, followed by a brief discussion on future biomedical applications.

Nanomaterials for bioprinting: functionalization of tissue-specific bioinks

2021 ◽  
Author(s):  
Andrea S. Theus ◽  
Liqun Ning ◽  
Linqi Jin ◽  
Ryan K. Roeder ◽  
Jianyi Zhang ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Biomedical Applications ◽  
Disease Modeling ◽  
Three Dimensional ◽  
3D Bioprinting ◽  
Significant Step ◽  
Printing Processes

Abstract Three-dimensional (3D) bioprinting is rapidly evolving, offering great potential for manufacturing functional tissue analogs for use in diverse biomedical applications, including regenerative medicine, drug delivery, and disease modeling. Biomaterials used as bioinks in printing processes must meet strict physiochemical and biomechanical requirements to ensure adequate printing fidelity, while closely mimicking the characteristics of the native tissue. To achieve this goal, nanomaterials are increasingly being investigated as a robust tool to functionalize bioink materials. In this review, we discuss the growing role of different nano-biomaterials in engineering functional bioinks for a variety of tissue engineering applications. The development and commercialization of these nanomaterial solutions for 3D bioprinting would be a significant step towards clinical translation of biofabrication.

scholarly journals Chitosans for Tissue Repair and Organ Three-Dimensional (3D) Bioprinting

Micromachines ◽  
2019 ◽  
Vol 10 (11) ◽  
pp. 765 ◽  
Author(s):  
Shenglong Li ◽  
Xiaohong Tian ◽  
Jun Fan ◽  
Hao Tong ◽  
Qiang Ao ◽  
...  
Keyword(s):  
Tissue Repair ◽  
Biomedical Applications ◽  
Large Scale ◽  
Scale Up ◽  
Three Dimensional ◽  
The Body ◽  
3D Bioprinting ◽  
Chemically Modified ◽  
Excellent Candidate ◽  
Complex Organ

Chitosan is a unique natural resourced polysaccharide derived from chitin with special biocompatibility, biodegradability, and antimicrobial activity. During the past three decades, chitosan has gradually become an excellent candidate for various biomedical applications with prominent characteristics. Chitosan molecules can be chemically modified, adapting to all kinds of cells in the body, and endowed with specific biochemical and physiological functions. In this review, the intrinsic/extrinsic properties of chitosan molecules in skin, bone, cartilage, liver tissue repair, and organ three-dimensional (3D) bioprinting have been outlined. Several successful models for large scale-up vascularized and innervated organ 3D bioprinting have been demonstrated. Challenges and perspectives in future complex organ 3D bioprinting areas have been analyzed.

scholarly journals Rational Design of Peptide-based Smart Hydrogels for Therapeutic Applications

2021 ◽  
Vol 9 ◽  
Author(s):  
Saurav Das ◽  
Debapratim Das
Keyword(s):  
Drug Delivery ◽  
Tissue Engineering ◽  
Wound Healing ◽  
Biomedical Applications ◽  
Rational Design ◽  
Soft Materials ◽  
Biological Molecules ◽  
3D Bioprinting ◽  
Significant Progress ◽  
Mechanical Stiffness

Peptide-based hydrogels have captivated remarkable attention in recent times and serve as an excellent platform for biomedical applications owing to the impressive amalgamation of unique properties such as biocompatibility, biodegradability, easily tunable hydrophilicity/hydrophobicity, modular incorporation of stimuli sensitivity and other functionalities, adjustable mechanical stiffness/rigidity and close mimicry to biological molecules. Putting all these on the same plate offers smart soft materials that can be used for tissue engineering, drug delivery, 3D bioprinting, wound healing to name a few. A plethora of work has been accomplished and a significant progress has been realized using these peptide-based platforms. However, designing hydrogelators with the desired functionalities and their self-assembled nanostructures is still highly serendipitous in nature and thus a roadmap providing guidelines toward designing and preparing these soft-materials and applying them for a desired goal is a pressing need of the hour. This review aims to provide a concise outline for that purpose and the design principles of peptide-based hydrogels along with their potential for biomedical applications are discussed with the help of selected recent reports.

Applications of Scanning Microscopy in Biomedical Research

1968 ◽  
Vol 26 ◽  
pp. 354-355
Author(s):  
T. L. Hayes
Keyword(s):  
Information Content ◽  
Biomedical Research ◽  
Biomedical Applications ◽  
Three Dimensional ◽  
Dental Enamel ◽  
Resolving Power ◽  
Whole Mount ◽  
Two Parameters ◽  
Content Information ◽  
Sectioned Tissue

Biomedical applications of the scanning electron microscope (SEM) have increased in number quite rapidly over the last several years. Studies have been made of cells, whole mount tissue, sectioned tissue, particles, human chromosomes, microorganisms, dental enamel and skeletal material. Many of the advantages of using this instrument for such investigations come from its ability to produce images that are high in information content. Information about the chemical make-up of the specimen, its electrical properties and its three dimensional architecture all may be represented in such images. Since the biological system is distinctive in its chemistry and often spatially scaled to the resolving power of the SEM, these images are particularly useful in biomedical research.In any form of microscopy there are two parameters that together determine the usefulness of the image. One parameter is the size of the volume being studied or resolving power of the instrument and the other is the amount of information about this volume that is displayed in the image. Both parameters are important in describing the performance of a microscope. The light microscope image, for example, is rich in information content (chemical, spatial, living specimen, etc.) but is very limited in resolving power.

scholarly journals Comparative Study of Silk Fibroin-Based Hydrogels and Their Potential as Material for 3-Dimensional (3D) Printing

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3887
Author(s):  
Watcharapong Pudkon ◽  
Chavee Laomeephol ◽  
Siriporn Damrongsakkul ◽  
Sorada Kanokpanont ◽  
Juthamas Ratanavaraporn
Keyword(s):  
3D Printing ◽  
Silk Fibroin ◽  
Biomedical Applications ◽  
Mechanical Stability ◽  
Three Dimensional ◽  
Structure Stability ◽  
3 Dimensional ◽  
Critical Technology ◽  
Box Structure ◽  
High Degree

Three-dimensional (3D) printing is regarded as a critical technology in material engineering for biomedical applications. From a previous report, silk fibroin (SF) has been used as a biomaterial for tissue engineering due to its biocompatibility, biodegradability, non-toxicity and robust mechanical properties which provide a potential as material for 3D-printing. In this study, SF-based hydrogels with different formulations and SF concentrations (1–3%wt) were prepared by natural gelation (SF/self-gelled), sodium tetradecyl sulfate-induced (SF/STS) and dimyristoyl glycerophosphorylglycerol-induced (SF/DMPG). From the results, 2%wt SF-based (2SF) hydrogels showed suitable properties for extrusion, such as storage modulus, shear-thinning behavior and degree of structure recovery. The 4-layer box structure of all 2SF-based hydrogel formulations could be printed without structural collapse. In addition, the mechanical stability of printed structures after three-step post-treatment was investigated. The printed structure of 2SF/STS and 2SF/DMPG hydrogels exhibited high stability with high degree of structure recovery as 70.4% and 53.7%, respectively, compared to 2SF/self-gelled construct as 38.9%. The 2SF/STS and 2SF/DMPG hydrogels showed a great potential to use as material for 3D-printing due to its rheological properties, printability and structure stability.

scholarly journals Artificial Tumor Microenvironments in Neuroblastoma

Cancers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1629
Author(s):  
Colin H. Quinn ◽  
Andee M. Beierle ◽  
Elizabeth A. Beierle
Keyword(s):  
Extracellular Matrix ◽  
Three Dimensional ◽  
Therapeutic Interventions ◽  
3D Bioprinting ◽  
Culture Studies ◽  
In Vivo Models ◽  
Novel Treatments ◽  
Tumor Microenvironments ◽  
Novel Approach

In the quest to advance neuroblastoma therapeutics, there is a need to have a deeper understanding of the tumor microenvironment (TME). From extracellular matrix proteins to tumor associated macrophages, the TME is a robust and diverse network functioning in symbiosis with the solid tumor. Herein, we review the major components of the TME including the extracellular matrix, cytokines, immune cells, and vasculature that support a more aggressive neuroblastoma phenotype and encumber current therapeutic interventions. Contemporary treatments for neuroblastoma are the result of traditional two-dimensional culture studies and in vivo models that have been translated to clinical trials. These pre-clinical studies are costly, time consuming, and neglect the study of cofounding factors such as the contributions of the TME. Three-dimensional (3D) bioprinting has become a novel approach to studying adult cancers and is just now incorporating portions of the TME and advancing to study pediatric solid. We review the methods of 3D bioprinting, how researchers have included TME pieces into the prints, and highlight present studies using neuroblastoma. Ultimately, incorporating the elements of the TME that affect neuroblastoma responses to therapy will improve the development of innovative and novel treatments. The use of 3D bioprinting to achieve this aim will prove useful in developing optimal therapies for children with neuroblastoma.

Three-Dimensional Printing Using a Maize Protein: Zein-Based Inks in Biomedical Applications

2021 ◽  
Author(s):  
Jorge Alfonso Tavares-Negrete ◽  
Alberto Emanuel Aceves-Colin ◽  
Delia Cristal Rivera-Flores ◽  
Gladys Guadalupe Díaz-Armas ◽  
Anne-Sophie Mertgen ◽  
...  
Keyword(s):  
Biomedical Applications ◽  
Three Dimensional ◽  
Three Dimensional Printing ◽  
Maize Protein ◽  
Dimensional Printing

scholarly journals Two-photon polymerization nanolithography technology for fabrication of stimulus-responsive micro/nano-structures for biomedical applications

2020 ◽  
Vol 9 (1) ◽  
pp. 1118-1136
Author(s):  
Zhenjia Huang ◽  
Gary Chi-Pong Tsui ◽  
Yu Deng ◽  
Chak-Yin Tang
Keyword(s):  
Biomedical Applications ◽  
Three Dimensional ◽  
Water Soluble ◽  
Fabrication Technology ◽  
Nano Fabrication ◽  
Two Photon ◽  
Nano Structures ◽  
Wide Range ◽  
Stimulus Responsive ◽  
Two Photon Polymerization

AbstractMicro/nano-fabrication technology via two-photon polymerization (TPP) nanolithography is a powerful and useful manufacturing tool that is capable of generating two dimensional (2D) to three dimensional (3D) arbitrary micro/nano-structures of various materials with a high spatial resolution. This technology has received tremendous interest in cell and tissue engineering and medical microdevices because of its remarkable fabrication capability for sophisticated structures from macro- to nano-scale, which are difficult to be achieved by traditional methods with limited microarchitecture controllability. To fabricate precisely designed 3D micro/nano-structures for biomedical applications via TPP nanolithography, the use of photoinitiators (PIs) and photoresists needs to be considered comprehensively and systematically. In this review, widely used commercially available PIs are first discussed, followed by elucidating synthesis strategies of water-soluble initiators for biomedical applications. In addition to the conventional photoresists, the distinctive properties of customized stimulus-responsive photoresists are discussed. Finally, current limitations and challenges in the material and fabrication aspects and an outlook for future prospects of TPP for biomedical applications based on different biocompatible photosensitive composites are discussed comprehensively. In all, this review provides a basic understanding of TPP technology and important roles of PIs and photoresists for fabricating high-precision stimulus-responsive micro/nano-structures for a wide range of biomedical applications.

Remarks on marine and continental biogeography: an areographical viewpoint

1994 ◽  
Vol 343 (1303) ◽  
pp. 71-78 ◽  
Keyword(s):  
Species Richness ◽  
Baltic Sea ◽  
Three Dimensional ◽  
Wide Spread ◽  
The Baltic Sea ◽  
Biogeographical Regions ◽  
The Baltic ◽  
The Way

Differences and similarities in the way marine and continental organisms occupy space are briefly reviewed. Among them, the ‘peninsula effect’ (the decline of species richness with distance from the source) is compared with the ‘bay effect’. Two cases, corals in Mochima Bay, Venezuela and fishes in the Baltic Sea, are presented as examples. The facts that the world’s oceans are larger, continuous and three-dimensional, with fewer evident geographical barriers than there are on land, explain why marine biogeographical regions are less welldefined and geographical ranges of marine taxa more wide-spread. I his generalization has, however, been questioned following recent findings of extremely rich and highly endemic benthic faunas. This problem is discussed using an index of cosmopolitanism to compare terrestrial and marine biotas.

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