scholarly journals Identification and Analysis of Exosomes by Surface-Enhanced Raman Spectroscopy

2019 ◽  
Vol 9 (6) ◽  
pp. 1135 ◽  
Author(s):  
Anastasiia Merdalimova ◽  
Vasiliy Chernyshev ◽  
Daniil Nozdriukhin ◽  
Polina Rudakovskaya ◽  
Dmitry Gorin ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
High Efficiency ◽  
High Sensitivity ◽  
Cancer Biomarkers ◽  
Surface Enhanced Raman Spectroscopy ◽  
Circulating Tumor Dna ◽  
Novel Approach ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

The concept of liquid biopsy has emerged as a novel approach for cancer screening, which is based on the analysis of circulating cancer biomarkers in body fluids. Among the various circulating cancer biomarkers, including Food and Drug Administration (FDA)-approved circulating tumor cells (CTC) and circulating tumor DNA (ctDNA), exosomes have attracted tremendous attention due to their ability to diagnose cancer in its early stages with high efficiency. Recently, surface-enhanced Raman spectroscopy (SERS) has been applied for the detection of cancer exosomes due to its high sensitivity, specificity, and multiplexing capability. In this article, we review recent progress in the development of SERS-based technologies for in vitro identification of circulating cancer exosomes. The accent is made on the detection strategies and interpretation of the SERS data. The problems of detecting cancer-derived exosomes from patient samples and future perspectives of SERS-based diagnostics are also discussed.

In Situ Surface-Enhanced Raman Spectroscopy Characterization of Electrocatalysis with Different Nanostructures

2021 ◽  
Vol 72 (1) ◽  
Author(s):  
Bao-Ying Wen ◽  
Qing-Qi Chen ◽  
Petar M. Radjenovic ◽  
Jin-Chao Dong ◽  
Zhong-Qun Tian ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
High Efficiency ◽  
Spectroscopic Characterization ◽  
Surface Enhanced Raman Spectroscopy ◽  
Annual Review ◽  
Publication Date ◽  
Oxygen Reduction Reactions ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

As energy demands increase, electrocatalysis serves as a vital tool in energy conversion. Elucidating electrocatalytic mechanisms using in situ spectroscopic characterization techniques can provide experimental guidance for preparing high-efficiency electrocatalysts. Surface-enhanced Raman spectroscopy (SERS) can provide rich spectral information for ultratrace surface species and is extremely well suited to studying their activity. To improve the material and morphological universalities, researchers have employed different kinds of nanostructures that have played important roles in the development of SERS technologies. Different strategies, such as so-called borrowing enhancement from shell-isolated modes and shell-isolated nanoparticle-enhanced Raman spectroscopy (SHINERS)-satellite structures, have been proposed to obtain highly effective Raman enhancement, and these methods make it possible to apply SERS to various electrocatalytic systems. Here, we discuss the development of SERS technology, focusing on its applications in different electrocatalytic reactions (such as oxygen reduction reactions) and at different nanostructure surfaces, and give a brief outlook on its development. Expected final online publication date for the Annual Review of Physical Chemistry, Volume 72 is April 2021. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

scholarly journals Surface-Enhanced Raman Spectroscopy for Cancer Immunotherapy Applications: Opportunities, Challenges, and Current Progress in Nanomaterial Strategies

Nanomaterials ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 1145 ◽  
Author(s):  
Shuvashis Dey ◽  
Matt Trau ◽  
Kevin M. Koo
Keyword(s):  
Raman Spectroscopy ◽  
Cancer Immunotherapy ◽  
Immune Checkpoint ◽  
Checkpoint Inhibitors ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Checkpoint Receptors

Cancer immunotherapy encompasses a variety of approaches which target or use a patient’s immune system components to eliminate cancer. Notably, the current use of immune checkpoint inhibitors to target immune checkpoint receptors such as CTLA-4 or PD-1 has led to remarkable treatment responses in a variety of cancers. To predict cancer patients’ immunotherapy responses effectively and efficiently, multiplexed immunoassays have been shown to be advantageous in sensing multiple immunomarkers of the tumor microenvironment simultaneously for patient stratification. Surface-enhanced Raman spectroscopy (SERS) is well-regarded for its capabilities in multiplexed bioassays and has been increasingly demonstrated in cancer immunotherapy applications in recent years. This review focuses on SERS-active nanomaterials in the modern literature which have shown promise for enabling cancer patient-tailored immunotherapies, including multiplexed in vitro and in vivo immunomarker sensing and imaging, as well as immunotherapy drug screening and delivery.

Multiplexed Microfluidic Surface-Enhanced Raman Spectroscopy

2007 ◽  
Vol 61 (10) ◽  
pp. 1116-1122 ◽  
Author(s):  
Nahla A. Abu-Hatab ◽  
Joshy F. John ◽  
Jenny M. Oran ◽  
Michael J. Sepaniak
Keyword(s):  
Raman Spectroscopy ◽  
Structural Information ◽  
Numerical Aperture ◽  
High Sensitivity ◽  
Surface Enhanced Raman Spectroscopy ◽  
Optical Extinction ◽  
Analytical Technique ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Working Distance

Over the past few decades, surface-enhanced Raman spectroscopy (SERS) has garnered respect as an analytical technique with significant chemical and biological applications. SERS is important for the life sciences because it can provide trace level detection, a high level of structural information, and enhanced chemical detection. However, creating and successfully implementing a sensitive, reproducible, and robust SERS active substrate continues to be a challenging task. Herein, we report a novel method for SERS that is based upon using multiplexed microfluidics (MMFs) in a polydimethylsiloxane platform to perform parallel, high throughput, and sensitive detection/identification of single or various analytes under easily manipulated conditions. A facile passive pumping method is used to deliver Ag colloids and analytes into the channels where SERS measurements are done under nondestructive flowing conditions. With this approach, SERS signal reproducibility is found to be better than 7%. Utilizing a very high numerical aperture microscope objective with a confocal-based Raman spectrometer, high sensitivity is achieved. Moreover, the long working distance of this objective coupled with an appreciable channel depth obviates normal alignment issues expected with translational multiplexing. Rapid evaluation of the effects of anion activators and the type of colloid employed on SERS performance are used to demonstrate the efficiency and applicability of the MMF approach. SERS spectra of various pesticides were also obtained. Calibration curves of crystal violet (non-resonant enhanced) and Mitoxantrone (resonant enhanced) were generated, and the major SERS bands of these analytes were observable down to concentrations in the low nM and sub-pM ranges, respectively. While conventional random morphology colloids were used in most of these studies, unique cubic nanoparticles of silver were synthesized with different sizes and studied using visible wavelength optical extinction spectrometry, scanning electron microscopy, and the MMF-SERS approach.

High efficiency screening of nine lipid-lowering adulterants in herbal dietary supplements using thin layer chromatography coupled with surface enhanced Raman spectroscopy

2017 ◽  
Vol 9 (10) ◽  
pp. 1595-1602 ◽  
Author(s):  
Qingxia Zhu ◽  
Mengyun Chen ◽  
Lu Han ◽  
Yongfang Yuan ◽  
Feng Lu
Keyword(s):  
Raman Spectroscopy ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Efficiency ◽  
Screening Method ◽  
Surface Enhanced Raman Spectroscopy ◽  
Lipid Lowering ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Layer Chromatography

A high efficiency screening method was developed to analyze lipid-lowering adulterants in complicated HDS systems.

ZnO–Ag hybrids for ultrasensitive detection of trinitrotoluene by surface-enhanced Raman spectroscopy

2014 ◽  
Vol 16 (28) ◽  
pp. 14706-14712 ◽  
Author(s):  
Xuan He ◽  
Hui Wang ◽  
Zhongbo Li ◽  
Dong Chen ◽  
Qi Zhang
Keyword(s):  
Raman Spectroscopy ◽  
Low Cost ◽  
High Sensitivity ◽  
Surface Enhanced Raman Spectroscopy ◽  
Ultrasensitive Detection ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Hybrid Substrates ◽  
Tnt Detection

Low-cost SERS sensors were fabricated by 4-ATP-functionalized ZnO–Ag hybrid substrates for TNT detection with high sensitivity, selectivity and reproducibility.

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