A novel approach to determine leucomalachite green and malachite green in fish fillets with surface-enhanced Raman spectroscopy (SERS) and multivariate analyses

2012 ◽  
Vol 43 (9) ◽  
pp. 1208-1213 ◽  
Author(s):  
Yuanyuan Zhang ◽  
Keqiang Lai ◽  
Jinglie Zhou ◽  
Xichang Wang ◽  
Barbara A. Rasco ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Malachite Green ◽  
Multivariate Analyses ◽  
Surface Enhanced Raman Spectroscopy ◽  
Fish Fillets ◽  
Novel Approach ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Leucomalachite Green

scholarly journals Detection of Triphenylmethane Drugs in Fish Muscle by Surface-Enhanced Raman Spectroscopy Coupled with Au-Ag Core-Shell Nanoparticles

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Lu Pei ◽  
Yiqun Huang ◽  
Chunying Li ◽  
Yuanyuan Zhang ◽  
Barbara A. Rasco ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Malachite Green ◽  
Crystal Violet ◽  
Bimetallic Nanoparticles ◽  
Limit Of Detection ◽  
Fish Muscle ◽  
Surface Enhanced Raman Spectroscopy ◽  
Vis Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Silver-coated gold bimetallic nanoparticles were synthesized and used as substrates for surface-enhanced Raman spectroscopy (SERS) in detecting prohibited triphenylmethane drugs (including crystal violet and malachite green) in fish muscle. The optical properties and physical properties of bimetallic nanospheres were characterized by UV-Vis spectroscopy and transmission electron microscopy. The optimal nanospheres selected had relatively uniform size (diameter: 33 ± 3 nm) with a silver layer coated on the surface of gold seed (diameter: 18 ± 2 nm). For both crystal violet and malachite green, characteristic SERS spectral features could be identified at concentration as low as 0.1 μg/L with these bimetallic nanospheres. Crystal violet and malachite green residues in fish muscle could also be detected at levels as low as 0.1 ng/g, which could meet the most restricted regulatory requirements for the limit of detection in terms of analytical methods for crystal violet or malachite green in fish muscle. This study provides a basis for applying SERS technology with bimetallic nanoparticles to the identification of trace amounts of prohibited substances in aquatic food products, and the methodology could be extended to analyses of other hazardous chemicals in complex food matrices like vegetables and meats.

scholarly journals Identification and Analysis of Exosomes by Surface-Enhanced Raman Spectroscopy

2019 ◽  
Vol 9 (6) ◽  
pp. 1135 ◽  
Author(s):  
Anastasiia Merdalimova ◽  
Vasiliy Chernyshev ◽  
Daniil Nozdriukhin ◽  
Polina Rudakovskaya ◽  
Dmitry Gorin ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
High Efficiency ◽  
High Sensitivity ◽  
Cancer Biomarkers ◽  
Surface Enhanced Raman Spectroscopy ◽  
Circulating Tumor Dna ◽  
Novel Approach ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

The concept of liquid biopsy has emerged as a novel approach for cancer screening, which is based on the analysis of circulating cancer biomarkers in body fluids. Among the various circulating cancer biomarkers, including Food and Drug Administration (FDA)-approved circulating tumor cells (CTC) and circulating tumor DNA (ctDNA), exosomes have attracted tremendous attention due to their ability to diagnose cancer in its early stages with high efficiency. Recently, surface-enhanced Raman spectroscopy (SERS) has been applied for the detection of cancer exosomes due to its high sensitivity, specificity, and multiplexing capability. In this article, we review recent progress in the development of SERS-based technologies for in vitro identification of circulating cancer exosomes. The accent is made on the detection strategies and interpretation of the SERS data. The problems of detecting cancer-derived exosomes from patient samples and future perspectives of SERS-based diagnostics are also discussed.

Bacterial detection and differentiation via direct volatile organic compound sensing with surface enhanced Raman spectroscopy

2017 ◽  
Author(s):  
Caitlin S. DeJong ◽  
David I. Wang ◽  
Aleksandr Polyakov ◽  
Anita Rogacs ◽  
Steven J. Simske ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Bacterial Infections ◽  
Direct Detection ◽  
Point Of Care ◽  
Surface Enhanced Raman Spectroscopy ◽  
E Coli ◽  
Recent Emergence ◽  
Surface Enhanced ◽  
Volatile Organic ◽  
Surface Enhanced Raman

Through the direct detection of bacterial volatile organic compounds (VOCs), via surface enhanced Raman spectroscopy (SERS), we report here a reconfigurable assay for the identification and monitoring of bacteria. We demonstrate differentiation between highly clinically relevant organisms: <i>Escherichia coli</i>, <i>Enterobacter cloacae</i>, and <i>Serratia marcescens</i>. This is the first differentiation of bacteria via SERS of bacterial VOC signatures. The assay also detected as few as 10 CFU/ml of <i>E. coli</i> in under 12 hrs, and detected <i>E. coli</i> from whole human blood and human urine in 16 hrs at clinically relevant concentrations of 10<sup>3</sup> CFU/ml and 10<sup>4</sup> CFU/ml, respectively. In addition, the recent emergence of portable Raman spectrometers uniquely allows SERS to bring VOC detection to point-of-care settings for diagnosing bacterial infections.

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