scholarly journals Water Jacket Systems for Temperature Control of Petri Dish Cell Culture Chambers

2019 ◽  
Vol 9 (4) ◽  
pp. 621 ◽  
Author(s):  
Samira Uharek ◽  
Sara Baratchi ◽  
Jiu Zhu ◽  
Majed Alshehri ◽  
Arnan Mitchell ◽  
...  
Keyword(s):  
Cell Culture ◽  
Temperature Gradient ◽  
Transition Period ◽  
Thermal Characteristics ◽  
Petri Dish ◽  
Sample Volume ◽  
Radial Temperature ◽  
Transient Conditions ◽  
Water Jacket ◽  
Stable Conditions

Water jacket systems are routinely used to control the temperature of Petri dish cell culture chambers. Despite their widespread use, the thermal characteristics of such systems have not been fully investigated. In this study, we conducted a comprehensive set of theoretical, numerical and experimental analyses to investigate the thermal characteristics of Petri dish chambers under stable and transient conditions. In particular, we investigated the temperature gradient along the radial axis of the Petri dish under stable conditions, and the transition period under transient conditions. Our studies indicate a radial temperature gradient of 3.3 °C along with a transition period of 27.5 min when increasing the sample temperature from 37 to 45 °C for a standard 35 mm diameter Petri dish. We characterized the temperature gradient and transition period under various operational, geometric, and environmental conditions. Under stable conditions, reducing the diameter of the Petri dish and incorporating a heater underneath the Petri dish can effectively reduce the temperature gradient across the sample. In comparison, under transient conditions, reducing the diameter of the Petri dish, reducing sample volume, and using glass Petri dish chambers can reduce the transition period.

scholarly journals ASSESSMENT OF HEAT TRANSFER IN FUEL CHANNEL OF INDIAN PHWR UNDER POSTULATED LARGE BREAK LOSS OF COOLANT ACCIDENT: EXPERIMENTAL AND NUMERICAL STUDY

2021 ◽  
Vol 247 ◽  
pp. 11003
Author(s):  
Ketan Ajay ◽  
Ravi Kumar ◽  
Akhilesh Gupta
Keyword(s):  
Heat Transfer ◽  
Temperature Gradient ◽  
Numerical Study ◽  
Safety Concern ◽  
Thermal Characteristics ◽  
Fission Products ◽  
Hydrogen Gas ◽  
Gas Generation ◽  
Radial Temperature ◽  
Fuel Bundle

The behaviour of the channel under postulated large break LOCA scenario had been a prime safety concern. The radiative heat transfer is predominant in a channel when the convective cooling environment is marred. The estimation of temperature distribution in the fuel pins at elevated temperature is essential from the point of view of hydrogen gas generation and release of fission products. In this paper, the thermal characteristics of a channel for Indian PHWR under critical break failure is studied using experimental and numerical techniques. The experiment is carried out on an Indian PHWR having a fuel bundle of 37-fuel elements. The temperature profiles for different parts of the simulated channel comprising of fuel pins, PT and CT are obtained under steady condition. The numerical analysis is also performed using ANSYS Fluent 19.0. From the study, it is found that there is a significant radial temperature gradient in the fuel bundle from the center ring to the outer ring. Also, no significant circumferential temperature gradient is observed in the fuel bundle, PT and CT.

The Effect of Modified Crucible Design and Seed Attachment on SiC Crystal Grown by PVT

2013 ◽  
Vol 740-742 ◽  
pp. 77-80
Author(s):  
Jung Young Jung ◽  
Sang Il Lee ◽  
Mi Seon Park ◽  
Doe Hyung Lee ◽  
Hee Tae Lee ◽  
...  
Keyword(s):  
Crystal Growth ◽  
Temperature Gradient ◽  
Single Crystals ◽  
Crystal Quality ◽  
Full Width ◽  
Half Maximum ◽  
Radial Temperature Gradient ◽  
Radial Temperature ◽  
Better Than

The present research was focused to investigate the effect of internal crucible design that influenced the 4H-SiC crystal growth onto a 6H-SiC seed by PVT method. The crucible design was modified to produce a uniform radial temperature gradient in the growth cell. The seed attachment was also modified with a use of polycrystalline SiC plate. The crystal quality of 4H-SiC single crystals grown in modified crucible and grown with modified seed attachment was revealed to be better than that of crystal grown in conventional crucible. The full width at half maximum (FWHM) values of grown SiC crystals in the conventional crucible, the modified seed attachment and the modified crucible were 285 arcsec, 134 arcsec and 128 arcsec, respectively. The micropipe density (MPD) of grown SiC crystals in the conventional crucible, the modified seed attachment and the modified crucible were 101ea/cm^2, 81ea/cm^2 and 42ea/cm^2, respectively.

scholarly journals Microfluidic chambers using fluid walls for cell biology

2018 ◽  
Vol 115 (26) ◽  
pp. E5926-E5933 ◽  
Author(s):  
Cristian Soitu ◽  
Alexander Feuerborn ◽  
Ann Na Tan ◽  
Henry Walker ◽  
Pat A. Walsh ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Biology ◽  
Mammalian Cells ◽  
Soft Lithography ◽  
Petri Dish ◽  
Microtiter Plate ◽  
The Media ◽  
Downstream Analysis ◽  
Microfluidic Chambers ◽  
Made In

Many proofs of concept have demonstrated the potential of microfluidics in cell biology. However, the technology remains inaccessible to many biologists, as it often requires complex manufacturing facilities (such as soft lithography) and uses materials foreign to cell biology (such as polydimethylsiloxane). Here, we present a method for creating microfluidic environments by simply reshaping fluids on a substrate. For applications in cell biology, we use cell media on a virgin Petri dish overlaid with an immiscible fluorocarbon. A hydrophobic/fluorophilic stylus then reshapes the media into any pattern by creating liquid walls of fluorocarbon. Microfluidic arrangements suitable for cell culture are made in minutes using materials familiar to biologists. The versatility of the method is demonstrated by creating analogs of a common platform in cell biology, the microtiter plate. Using this vehicle, we demonstrate many manipulations required for cell culture and downstream analysis, including feeding, replating, cloning, cryopreservation, lysis plus RT-PCR, transfection plus genome editing, and fixation plus immunolabeling (when fluid walls are reconfigured during use). We also show that mammalian cells grow and respond to stimuli normally, and worm eggs develop into adults. This simple approach provides biologists with an entrée into microfluidics.

Study on the transient temperature distribution of new conical friction plate under sliding friction

2021 ◽  
pp. 095440702110696
Author(s):  
Yanzhong Wang ◽  
Peng Liu
Keyword(s):  
Temperature Distribution ◽  
Temperature Gradient ◽  
Temperature Rise ◽  
Friction Surface ◽  
Sliding Friction ◽  
Conical Surface ◽  
Transient Temperature ◽  
Radial Temperature Gradient ◽  
Radial Temperature ◽  
Friction Plate

Conical friction surface is a novel configuration for friction plate in transmission. Numerical FEA models for transient heat transfer and distribution of conically grooved friction plate have been established to investigate the thermal behavior of the conical surface with different configurations. The finite element method is used to obtain the numerical solution, the temperature test data of conical surface are obtained by the friction test rig. In order to study and compare the temperature behavior of conically grooved friction plate, several three-dimensional transient temperature models are established. The heat generated on the friction interface during the continuous sliding process is calculated. Two different pressure conditions were defined to evaluate the influence of different load conditions on temperature rise and the effects of conical configuration parameters on surface temperature distribution are investigated. The results show that the radial temperature gradient on conical friction surface is obvious. The uniform pressure condition could be used when evaluating the temperature rise of conically grooved friction plate. The increase of the cone height could improve the radial temperature gradient of the conically grooved friction plate.

THERMAL CHARACTERISTICS OF FOAMS AND DISCHARGE FROM FIRE-EXTINGUISHMENT FOAM SPRAY NOZZLE

2009 ◽  
Vol 23 (03) ◽  
pp. 381-384
Author(s):  
YOUN-JEA KIM ◽  
JIN-SOO PYO
Keyword(s):  
Heat Flux ◽  
Temperature Gradient ◽  
Thermal Characteristics ◽  
Expansion Ratio ◽  
Experimental Results ◽  
Orifice Diameter ◽  
Spray Nozzle ◽  
Foam Expansion ◽  
Fire Extinguishment ◽  
Air Hole

To evaluate the performance of discharged foam agents used to protect structures from heat and fire damages, the thermal characteristics of fire-extinguishment foams were experimentally investigated. Especially, two different parameters of a spray nozzle, that is, the number of air holes and the orifice diameter, were considered. A simple repeatable test for fire-extinguishment foams subjected to fire radiation was performed. Experimental results showed that the expansion ratio of the discharged foam with the small orifice throat ( d 0= 9.5 mm ) and opened air hole ( N h =9) was large. Results also showed that although the temperature gradient in the foam increased as the foam expansion ratio is increased, it remained constant as the intensity of heat flux increased.

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