scholarly journals Synthesis and Encapsulation of a New Zinc Phthalocyanine Photosensitizer into Polymeric Nanoparticles to Enhance Cell Uptake and Phototoxicity

2019 ◽  
Vol 9 (3) ◽  
pp. 401 ◽  
Author(s):  
Nahid Mehraban ◽  
Phillip Musich ◽  
Harold Freeman
Keyword(s):  
Photodynamic Therapy ◽  
Cancer Cells ◽  
Parent Compound ◽  
A549 Cells ◽  
Cancer Cell Line ◽  
Lung Cancer Cell Line ◽  
Cell Uptake ◽  
Zinc Phthalocyanine ◽  
Human Lung Cancer ◽  
Surrounding Tissue

Efforts to enhance the utility of photodynamic therapy as a non-invasive method for treating certain cancers have often involved the design of dye sensitizers with increased singlet oxygen efficiency. More recently, however, sensitizers with greater selectivity for tumor cells than surrounding tissue have been targeted. The present study provides an approach to the modification of the known photosensitizer zinc phthalocyanine (ZnPc), to enhance its solubility and delivery to cancer cells. Targeting a photosensitizer to the site of action improves the efficacy of the sensitizer in photodynamic therapy. In this work we used PLGA-b-PEG to encapsulate a new zinc phthalocyanine derivative, 2(3), 9(10), 16(17), 23(24)-tetrakis-(4’-methyl-benzyloxy) phthalocyanine zinc(II) (ZnPcBCH3), to enhance uptake into A549 cells, a human lung cancer cell line. ZnPcBCH3 exhibited the same photochemical properties as the parent compound ZnPc but gave increased solubility in organic solvents, which allowed for efficient encapsulation. In addition, the encapsulated dye showed a near 500-fold increase in phototoxicity for A549 cancer cells compared to free dye.

Synthesis and in vitro cytotoxicity evaluation of ruthenium polypyridyl-sensitized paramagnetic titania nanoparticles for photodynamic therapy

RSC Advances ◽  
2016 ◽  
Vol 6 (53) ◽  
pp. 47520-47529 ◽  
Author(s):  
Mohammad H. Sakr ◽  
Najeeb M. Halabi ◽  
Leen N. Kalash ◽  
Sara I. Al-Ghadban ◽  
Mayyasa K. Rammah ◽  
...  
Keyword(s):  
A549 Cells ◽  
Cancer Cell Line ◽  
In Vitro Cytotoxicity ◽  
Lung Cancer Cell Line ◽  
Titania Nanoparticles ◽  
Human Lung Cancer ◽  
Type I ◽  
Dye Sensitized ◽  
Photo Dynamic Therapy

We demonstrate the effective cytotoxic properties of a dye-sensitized metal oxide in an in vitro model of a human lung cancer cell line (A549 cells) upon light irradiation, where a type I mechanism photo-dynamic therapy is realized exclusively.

scholarly journals Human Lung Cancer Cell Line A-549 ATCC Is Differentially Affected by Supranutritional Organic and Inorganic Selenium

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Lérida Liss Flores Villavicencio ◽  
Gustavo Cruz-Jiménez ◽  
Gloria Barbosa-Sabanero ◽  
Carlos Kornhauser-Araujo ◽  
M. Eugenia Mendoza-Garrido ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Growth ◽  
Cancer Cells ◽  
Cancer Cell Line ◽  
Lung Cancer Cell Line ◽  
Lung Cancer Cells ◽  
Human Lung Cancer ◽  
Lung Cancer Cell ◽  
Nuclear Fragmentation ◽  
Inorganic Selenium

The effects of organic and inorganic forms of selenium (Se) on human cells have been extensively studied for nutritional concentrations; however, to date, little is known about the potential toxicity at supranutritional levels. In the present study we determined the effects of sodium selenite (SSe) and selenomethionine (SeMet) on cell growth and intracellular structures in lung cancer cells exposed at Se concentrations between 0 and 3 mM. Our results showed that SSe affected cell growth more rapidly than SeMet (24 h and 48 h, resp.). After 24 h of cells exposure to 0.5, 1.5, and 3 mM SSe, cell growth was reduced by 10, 50, and 60%, as compared to controls. After 48 h, nuclear fragmentation was evident in cells exposed to SSe, suggesting an induction to cell death. In contrast, SeMet did not affect cell proliferation, and the cells were phenotypically similar to controls. Microtubules and microfilaments structures were also affected by both Se compounds, again SSe being more toxic than SeMet. To our knowledge, this is the first report on the differential effects of organic and inorganic Se in supranutritional levels in lung cancer cells.

The Rhizome of Trillium tschonoskii Maxim. Extract Induces Apoptosis in Human Lung Cancer Cells

2011 ◽  
Vol 66 (9-10) ◽  
pp. 477-484 ◽  
Author(s):  
Wenfeng Huang ◽  
Kun Zou ◽  
Bin Xiong
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Human Lung ◽  
Cancer Cell Line ◽  
Lung Cancer Cell Line ◽  
Human Lung Cancer ◽  
Lung Cancer Cell ◽  
Butanol Extract ◽  
Human Lung Cancer Cells ◽  
Trillium Tschonoskii

Trillium tschonoskii Maxim. has been used to treat several diseases including cancers in folk medicine. However, the mechanisms responsible for T. tschonoskii extract-induced apoptosis are not clear. This study was mainly undertaken to identify the major biochemical changes in a lung cancer cell line upon treatment with an T. tschonoskii extract (TTME), and to investigate the functional relationship between these changes. The n-butanol extract was used to evaluate the mechanism of induction of apoptosis in A549 human lung cancer cells and its effects on mitochondrial function and production of reactive oxygen species (ROS). The n-butanol extract of T. tschonoskii has cytotoxic, antiproliferative, and morphological effects on the lung cancer cell line. T. tschonoskii mainly leads to apoptosis of cancer cells with a concomitant increase in the release of cytochrome c and a loss of mitochondrial membrane potential in a dose-dependent manner. A rapid increase in the level of intracellular ROS and an accumulation of cells in the G2/M and S phase of the cell cycle were also observed in treated cells. These observations suggest that the n-butanol extract of T. tschonoskii has promising anticancer activities, which could be useful in cancer treatment.

The effect of antisense oligodeoxynucleotides targeting Aurora A kinase on cell proliferation and chemosensitivity to paclitaxel in human lung cancer cell line A549

2007 ◽  
Vol 25 (18_suppl) ◽  
pp. 21147-21147
Author(s):  
R. Meng ◽  
G. Wu ◽  
K. Y. Yang ◽  
J. Cheng
Keyword(s):  
Lung Cancer ◽  
Cell Line ◽  
A549 Cells ◽  
Cancer Cell Line ◽  
Lung Cancer Cell Line ◽  
Human Lung Cancer ◽  
Aurora A ◽  
Lung Cancer Cell ◽  
Mrna And Protein Expression ◽  
Cell Inhibition

21147 Background: Aurora kinases representing a family of evolutionarily conserved mitotic serine/threonine kinases have been found elevated in lung andenocarcinoma cell line A549. It is suggested that the overexpression of Aurora A contributes to the carcinogenesis, chromosomal instability (CIN), and de-differentiation of lung cancers. To address its possibility as a therapeutic target for lung cancer, we employed the antisense oligodeoxynucleotide (ASODN) technique to inhibit Aurora A expression and investegate its effect on tumor growth and cell cycle of A549, as well as the chemosensitivity of paclitaxel. Methods: Aurora A ASODN was synthesized and transfected into A549 cells by lipofectAMINE 2000.Aurora A mRNA and protein expression were examined by reverse transcription -polymerase chain reaction (RT-PCR) and Western blot respectively.Cell cycle distribution was observed by flow cytometer.MTT assay was used to evaluate cell inhibition ratio before and after transfection. Results: The proliferation of the A549 cells was inhibited by Aurora A ASODN dose and time dependently. It was also observed that the IC50 of A549 cells after 48 hours’ treatment of ASODN was about 300nmol/L and under such circumstances, the Aurora A mRNA and protein expression significantly decreased (P<0.05), along with the induction of accumulation of cells in S phase and the G2-M transition. Furhermore, cell inhibition ratio of the combination of Aurora A ASODN and paclitaxel was higher significantly than paclitaxel(P<0.05) or Aurora A ASODN alone (P<0.05). Conclusions: Inhibition of Aurora A expression can results in the suppression of cell growth and chemosensitizing activity to paclitaxel in human lung cancer cell line A549. No significant financial relationships to disclose.

scholarly journals Phytochemical Profiles and Bioactivities of Cake Tea Leaves Obtained From the Same Cultivar: A Comparative Analysis

2020 ◽  
Vol 15 (8) ◽  
pp. 1934578X2094550
Author(s):  
Lingli Sun ◽  
Qiuhua Li ◽  
Limin Xiang ◽  
Xingfei Lai ◽  
Wenji Zhang ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Health Benefits ◽  
A549 Cells ◽  
Cancer Cell Line ◽  
Lung Cancer Cell Line ◽  
Human Lung Cancer ◽  
Tea Leaves ◽  
Excellent Health ◽  
Total Free Amino Acids ◽  
Phytochemical Profiles

Cake tea, a traditional beverage of China, has excellent health benefits. Our study investigated the phytochemical profiles, antioxidant, and antiproliferative activities of cake tea leaves, which were obtained from the same cultivar and processed at different intervals. The effects of bioactive compounds and antioxidant activities of 4 cake tea varieties on a human lung cancer cell line (A549 cells) were systematically examined. The content of total polyphenol, an active ingredient of tea, was significantly higher in green cake teas (14.0% ± 0.4a) than their black (4.8 ± 0.3c), yellow (10.0 ± 0.6b), and white (8.8 ± 0.5b) counterparts. Likewise, the content of total free amino acids in green cake tea (3.6% ± 0.5a) was significantly higher than the other tea varieties. Our results indicated that the extent of fermentation of tea leaves could decrease the antioxidant activities of cake tea leaves. Furthermore, the white tea cake variety demonstrated the maximum antiproliferative activity on A549 cells as opposed to other types of cake tea leaves. Such an observation allows future researchers to narrow down their focus on using specific cake tea types (sourced from the same cultivar) that provide the maximum health benefits.

scholarly journals Photodynamic therapy on a human lung cancer cell line a549 with new bodipy photosensitizers

2019 ◽  
Author(s):  
Soraia Pedro ◽  
Mafalda Laranjo ◽  
António Aguiar ◽  
Maria Filomena Botelho ◽  
Abilio J.F.N. Sobral
Keyword(s):  
Lung Cancer ◽  
Photodynamic Therapy ◽  
Cell Line ◽  
Cancer Cell ◽  
Human Lung ◽  
Cancer Cell Line ◽  
Lung Cancer Cell Line ◽  
Human Lung Cancer ◽  
Lung Cancer Cell ◽  
Human Lung Cancer Cell

scholarly journals Phytochemical Analysis and Anticancer Activity of Azadirachta Indica Ethanolic Extract against A549 Human Lung Cancer Cell Line

2021 ◽  
Vol 2 (4) ◽  
pp. 280-285
Author(s):  
MS Azhagu
Keyword(s):  
Azadirachta Indica ◽  
Human Lung ◽  
A549 Cells ◽  
Cancer Cell Line ◽  
Ethanolic Extract ◽  
Lung Cancer Cell Line ◽  
Human Lung Cancer ◽  
Phytochemical Analysis ◽  
Lung Cancer Cell ◽  
Malignant Growth

Azadirachta indica phytochemicals are found to have against malignant growth and hostile to bacterial properties. In the specific examination, the coupling proficiency of five mixes that are available in the Azadirachta indica with all the eleven proteins through in silico techniques was completed. Plant removes ensure against harmful compound instigated injury by expanding the body’s degrees of cell reinforcement particles, for example, glutathione, and improving the action of cancer prevention agent chemicals. A549 cells treated with Azadirachta indica ethanolic separate in various hours (6, 12, 24 and 36 hours) after the 36 hours the cells development are controlled. As there are re-established interests in home grown based meds to hinder the results of manufactured medications, Azadirachta Indica L. a leaf contains phytochemical intensifies having all the more free revolutionary rummaging just as anticancer exercises.

scholarly journals The effect of Thymbra spicata extract and its bioactive component Thymol on non-small-cells lung cancer cell line A549

2021 ◽  
Author(s):  
Ardeshir Moayeri ◽  
Shahram Mohammadpour ◽  
Naser abbasi ◽  
Ali Aidy ◽  
Elahe Karimi ◽  
...  
Keyword(s):  
Lung Cancer ◽  
A549 Cells ◽  
Cancer Cell Line ◽  
Lung Cancer Cell Line ◽  
Small Cells ◽  
Human Lung Cancer ◽  
Ros Generation ◽  
Bioactive Component ◽  
High Concentrations ◽  
Thymbra Spicata

IntroductionAlternative medicine is important in cancer treatment. The apoptotic effect of Thymol and extracted Thymol from Thymbra spicata on non-small-cells lung cancer was studied.Material and methodsThymol was evaluated in Thymbra spicata extract by HPCL. Cell viability was assessed by MTT method. DCF and flu3-AM probe was used for ROS and cai2+ analysis, respectively. Western blotting was performed to measure NOX2 and Bax/Bcl-2 ratio.ResultsObtained data showed that Thymol was 1.51 mg/g in Thymbra spicata extract. Treatment with Thymol and extracted Thymol from Thymbra spicata resulted in cell death at high concentrations [LC50= 111±4.5 and 119±5.2 μM, respectively]. Subsequently, Thymbra spicata extract and its bioactive component increased ROS and Cai2+ production, NOX2, and Bax/Bcl-2 ratio.ConclusionsThis study revealed the anticancer effects of Thymol and Thymbra spicata extract on non-small-cells lung cancer and at least part of that effect was related to the increase in the NOX2 and Bax/Bcl-2 ratio. Our results demonstrated that TSE and Thymol at high concentrations (180, 120, and 80 μM) decreased the growth of A549 cells. It appeared that cytotoxic activity was exerted through activation of NOX2, ROS generation, increase in Cai2+, and Bax/Bcl-2 ratio. Present results demonstrated that TSE and thymol may be potential therapeutic agents for human lung cancer.

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