scholarly journals Studies of the Variability of Sugars, Vitamin C, and Chlorophylls in Differently Fermented Organic Leaves of Willowherb (Chamerion angustifolium (L.) Holub)

2021 ◽  
Vol 11 (21) ◽  
pp. 9891
Author(s):  
Marius Lasinskas ◽  
Elvyra Jariene ◽  
Nijole Vaitkeviciene ◽  
Ausra Blinstrubiene ◽  
Barbara Sawicka ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Vitamin C ◽  
High Performance ◽  
Functional Foods ◽  
Solid Phase ◽  
Principal Component ◽  
Total Vitamin ◽  
Chamerion Angustifolium ◽  
Fermentation Conditions

There is currently an increasing interest in functional foods and herbs as an opportunity to enrich one’s diet and at the same time improve one’s health. One of such plants is willowherb (Chamerion angustifolium (L.) Holub), which is rich not only in polyphenols, carotenoids, but also in sugars, chlorophylls, and vitamin C. This work purpose was to determine the effect of solid-phase fermentation (SPF) on changes in sugars, chlorophylls, and vitamin C under different fermentation conditions. Willowherb leaves were fermented for various durations (24, 48, and 72 h), in anaerobic and aerobic terms. The determination of sugars, chlorophylls, and vitamin C was done using high-performance liquid chromatography (HPLC) coupled to a spectrometer UV-VIS. The principal component analysis (PCA) was done to estimate the relationships between the different fermentation conditions (methods, as well as duration) and 10 parameters. The study showed: the biggest amounts of total chlorophylls and sugars were present after 72 h of anaerobic SPF, but the amount of total vitamin C was higher in unfermented willowherb leaves. In summary, SPF could be applied to modify chlorophylls and sugar quantities in willowherb organic leaves.

scholarly journals Quantitative Analysis in Combination with Fingerprint Technology and Chemometric Analysis Applied for Evaluating Six Species of Wild Paris Using UHPLC-UV-MS

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Yuangui Yang ◽  
Ji Zhang ◽  
Hang Jin ◽  
Jinyu Zhang ◽  
Yuanzhong Wang
Keyword(s):  
Principal Component Analysis ◽  
Quantitative Analysis ◽  
High Performance ◽  
Yunnan Province ◽  
Principal Component ◽  
Total Content ◽  
Similarity Analysis ◽  
Fast Method ◽  
Paris Polyphylla

A fast method was developed by ultra high performance liquid chromatography (UHPLC) for simultaneous determination of polyphyllin I and polyphyllin II. Chemometric analyses including principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) based on UHPLC chromatography were used to evaluate 38 batches from six species of Paris. Variable importance of projection was applied to select important peaks. Meanwhile, similarity analysis of UHPLC fingerprint was used to evaluate the sample of Paris polyphylla yunnanensis (PPY) and P. axialis (PA). The results indicated that the total content of saponins in PPY and PA collected from Baoshan City of Yunnan Province above 8.07 mg/g was stronger than that from other areas of the rest of species. PLS-DA showed better performance than PCA with regard to classifying the samples. Retention time during 20–27 minutes of UHPLC was screened as significant peak for distinguishing Paris of different species and original geography. All of PPY and PA with similarity value were more than 0.80. It indicated that quantitative analysis combined with chemometric and similarity analyses could evaluate the different species of Paris effectively and comprehensively.

scholarly journals Effect of Different Durations of Solid-Phase Fermentation for Fireweed (Chamerion angustifolium (L.) Holub) Leaves on the Content of Polyphenols and Antioxidant Activity In Vitro

Molecules ◽  
2020 ◽  
Vol 25 (4) ◽  
pp. 1011 ◽  
Author(s):  
Marius Lasinskas ◽  
Elvyra Jariene ◽  
Nijole Vaitkeviciene ◽  
Ewelina Hallmann ◽  
Katarzyna Najman
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Solid Phase ◽  
Principal Component ◽  
Biologically Active ◽  
Chamerion Angustifolium ◽  
Oenothein B ◽  
And Control

Fireweed has recently been recognized as a plant with high antioxidant potential and phenolic content. Its leaves can be fermented to prepare an infusion with ideal antioxidant activity. The aim of this study was to investigate and to determine the influence of solid-phase fermentation of different durations on the variation of polyphenols in the leaves of fireweed. Laboratory experiments were conducted in 2017–2018. The leaves of fireweed, naturally growing, were fermented for different periods of time: not fermented (control) and fermented for 24 and 48 h. The evaluation of polyphenols and antioxidant activity in leaves was performed using high- performance liquid chromatography (HPLC). Additionally, principal component analysis was used to characterize differences in bioactive compounds between fireweed samples fermented at different durations. Solid-phase fermented leaves were characterized by higher contents of oenothein B, quercetin and benzoic acid but had lower contents of quercetin-3-O-rutinoside, luteolin and chlorogenic and gallic acids. Antioxidant activity in short- (24 h) and long-term (48 h) fermentation (compared to control) gave the highest level of regression in 2017, but in 2018 the effect was observed only with short-term fermentation and control. In conclusion, solid-phase fermentation can be used to modulate biologically active compounds in fireweed leaves.

Multiwalled carbon nanotube-dispersive solid-phase extraction followed by high performance capillary electrophoresis for simultaneous determination of six adulterants in antihypertensive functional foods

2015 ◽  
Vol 7 (2) ◽  
pp. 543-550 ◽  
Author(s):  
Li Zeng ◽  
Xin Wu ◽  
Yongxin Li ◽  
Dan Lu ◽  
Chengjun Sun
Keyword(s):  
Capillary Electrophoresis ◽  
Carbon Nanotube ◽  
High Performance ◽  
Functional Foods ◽  
Solid Phase ◽  
Phase Extraction ◽  
Dispersive Solid Phase Extraction ◽  
Multiwalled Carbon ◽  
High Performance Capillary Electrophoresis

A novel, simple and accurate high performance capillary electrophoresis method after multiwalled carbon nanotube-dispersive solid-phase extraction was developed for simultaneous determination of hydrochlorothiazide (HCT), chlortalidone (CTD), indapamide (IDP), reserpine (RSP), nifedipine (NDP) and valsartan (VST) in antihypertensive functional foods.

scholarly journals Determination of Camelina Oil Sterol Composition and Its Application for Authenticity Studies

2020 ◽  
Vol 67 (4) ◽  
pp. 1163-1171
Author(s):  
Zala Kolenc ◽  
Tanja Potočnik ◽  
Urban Bren ◽  
Iztok Jože Košir
Keyword(s):  
Principal Component Analysis ◽  
Gas Chromatography ◽  
Solid Phase ◽  
Principal Component ◽  
Sterol Composition ◽  
Phase Extraction ◽  
Botanical Origin ◽  
Camelina Oil ◽  
Soya Oil

Camelina oil has a high sterol concentration and is rather expensive compared to other vegetable oils. Because of its higher price, it is often adulterated by the addition of other, cheaper oils. This study was performed to validate a method for sterol determination in camelina oil, enabling the detection of camelina oil adulteration. Sterol levels in camelina oil samples were determined by gas chromatography after saponification and solid phase extraction. The method was validated, and the results proved that the chosen method is specific and selective, repeatable and accurate. The quantitatively assessed average contents of sterols in camelina oil samples of Slovenian origin were 21.4 mg 100 g–1 for brassicasterol, 153.6 mg 100 g–1 for campesterol, 3.9 mg 100 g–1 for stigmasterol, and 447.0 mg 100 g–1 for β-sitosterol. Results of camelina oil authenticity studies regarding botanical origin, performed by Principal Component Analysis (PCA) and Regularized Discriminant Analysis (RDA) enabled us to differentiate 100 % camelina oils from camelina oils adulterated with 10%–40% added sunflower, rapeseed or soya oil.

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