scholarly journals Quantitative Analysis in Combination with Fingerprint Technology and Chemometric Analysis Applied for Evaluating Six Species of Wild Paris Using UHPLC-UV-MS

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Yuangui Yang ◽  
Ji Zhang ◽  
Hang Jin ◽  
Jinyu Zhang ◽  
Yuanzhong Wang
Keyword(s):  
Principal Component Analysis ◽  
Quantitative Analysis ◽  
High Performance ◽  
Yunnan Province ◽  
Principal Component ◽  
Total Content ◽  
Similarity Analysis ◽  
Fast Method ◽  
Paris Polyphylla

A fast method was developed by ultra high performance liquid chromatography (UHPLC) for simultaneous determination of polyphyllin I and polyphyllin II. Chemometric analyses including principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) based on UHPLC chromatography were used to evaluate 38 batches from six species of Paris. Variable importance of projection was applied to select important peaks. Meanwhile, similarity analysis of UHPLC fingerprint was used to evaluate the sample of Paris polyphylla yunnanensis (PPY) and P. axialis (PA). The results indicated that the total content of saponins in PPY and PA collected from Baoshan City of Yunnan Province above 8.07 mg/g was stronger than that from other areas of the rest of species. PLS-DA showed better performance than PCA with regard to classifying the samples. Retention time during 20–27 minutes of UHPLC was screened as significant peak for distinguishing Paris of different species and original geography. All of PPY and PA with similarity value were more than 0.80. It indicated that quantitative analysis combined with chemometric and similarity analyses could evaluate the different species of Paris effectively and comprehensively.

scholarly journals Simultaneous Determination of Six Chromones in Saposhnikoviae Radix via Quantitative Analysis of Multicomponents by Single Marker

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Jinhua Zhang ◽  
Luxiao Chen ◽  
Junna Qiu ◽  
Yu Zhang ◽  
Lu Wang ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Simultaneous Determination ◽  
Principal Component ◽  
Total Content ◽  
Quality Standard ◽  
Bioactive Constituents ◽  
External Standard Method ◽  
Single Marker ◽  
Saposhnikoviae Radix

A method, quantitative analysis of multicomponents by single marker (QAMS), was established and fully verified based on high-performance liquid chromatography (HPLC) for simultaneous determination of six chromone indicators of Saposhnikoviae Radix (SR). In the present study, cimifugin (C), 5-O-methylvisamminol (V), hamaudol (H), and their corresponding glycosides, prim-O-glucosylcimifugin (GC), 4′-O-β-D-glucosyl-5-O-methylvisamminol (GV), and sec-O-glucosylhamaudol (GH), were selected as bioactive constituents and indicators for the quality evaluation of SR. GV was chosen as the unique reference standard, and relative correction factors (RCF) between GV and the other five chromones were calculated. The feasibility of QAMS for the analysis of chromones was investigated by comparing with the traditional external standard method (ESM). Furthermore, the method was proven to have accuracy (96.98%–102.50%), repeatability (RSD <3%), stability (RSD <3%), precision (RSD <3%), and desirable linearity (R2 ≧0.9999). Subsequently, 55 batches of commercial SR from different regions were determined by QAMS, and their contents were analyzed by principal component analysis (PCA), correlation analysis, and hierarchical cluster analysis (HCA), respectively. Based on the results, a more refined quality standard of commercial SR was proposed: SR was qualified when the total contents of six chromones were greater than 3 mg·g−1. Furthermore, SR could initially be regarded as a superior medicine when it satisfied both conditions at the same time: the total content of GC, C, GV, V, GH, and H was greater than 8 mg·g−1, and the proportion of the total content of C, V, and H was greater than 10%. This study demonstrated that the quality of SR could be successfully evaluated by the developed QAMS method; meanwhile, valuable information was provided for improving the quality standard of SR.

scholarly journals Studies of the Variability of Sugars, Vitamin C, and Chlorophylls in Differently Fermented Organic Leaves of Willowherb (Chamerion angustifolium (L.) Holub)

2021 ◽  
Vol 11 (21) ◽  
pp. 9891
Author(s):  
Marius Lasinskas ◽  
Elvyra Jariene ◽  
Nijole Vaitkeviciene ◽  
Ausra Blinstrubiene ◽  
Barbara Sawicka ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Vitamin C ◽  
High Performance ◽  
Functional Foods ◽  
Solid Phase ◽  
Principal Component ◽  
Total Vitamin ◽  
Chamerion Angustifolium ◽  
Fermentation Conditions

There is currently an increasing interest in functional foods and herbs as an opportunity to enrich one’s diet and at the same time improve one’s health. One of such plants is willowherb (Chamerion angustifolium (L.) Holub), which is rich not only in polyphenols, carotenoids, but also in sugars, chlorophylls, and vitamin C. This work purpose was to determine the effect of solid-phase fermentation (SPF) on changes in sugars, chlorophylls, and vitamin C under different fermentation conditions. Willowherb leaves were fermented for various durations (24, 48, and 72 h), in anaerobic and aerobic terms. The determination of sugars, chlorophylls, and vitamin C was done using high-performance liquid chromatography (HPLC) coupled to a spectrometer UV-VIS. The principal component analysis (PCA) was done to estimate the relationships between the different fermentation conditions (methods, as well as duration) and 10 parameters. The study showed: the biggest amounts of total chlorophylls and sugars were present after 72 h of anaerobic SPF, but the amount of total vitamin C was higher in unfermented willowherb leaves. In summary, SPF could be applied to modify chlorophylls and sugar quantities in willowherb organic leaves.

scholarly journals Current Approaches to Valerian Tincture Standardisation in Terms of Assay

2019 ◽  
Vol 9 (4) ◽  
pp. 265-271
Author(s):  
N. P. Antonova ◽  
E. P. Shefer ◽  
A. M. Kalinin ◽  
N. E. Semenova ◽  
S. S. Prokhvatilova ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Quantitative Determination ◽  
High Performance ◽  
Test Procedure ◽  
Total Content ◽  
Reference Standard ◽  
Active Ingredients ◽  
Test Procedures ◽  
Valerenic Acid

The quality control of the «Valerian rhizome and roots» herbal substance is carried out using high performance liquid chromatography (HPLC) according to the State Pharmacopoeia of the Russian Federation, XIV edition. The quantitative analysis of the active ingredients in valerian tincture is performed using a non-specific and non-selective spectrophotometric method. Therefore, it is important to introduce in Russia a more modern test procedure for quantitative determination of active ingredients in valerian tincture.The aim of the study was to develop a selective and sensitive HPLC procedure for quantitative determination of the total content of sesquiterpenic acids, expressed as valerenic acid, for the purpose of valerian tincture standardisation.Materials and methods: valerian tincture samples produced by seven Russian manufacturers were used as test samples, and valerenic acid was used as the reference standard. The quantitative analysis of the active ingredients was performed by two methods: spectrophotometry at 512 nm following the reaction of valerenic acid ethylester with hydroxyalamine and ferric chloride, and by HPLC using a Nucleosil C18 column, 125×4.6 mm, 5 µm particle size, in gradient elution mode, with detection at 220 nm.Results: the spectrophotometric technique was shown to be insufficiently specific. The authors of the study validated the chromatographic test procedure, established system suitability criteria, and compared the results obtained by the two test procedures. They also determined a tentative standard of the total content of sesquiterpenic acids, expressed as valerenic acid, obtained by HPLC.Conclusions: the HPLC assay developed for quantitative determination of active ingredients in valerian tincture is more specific as compared to the spectrophotometric technique, as the sum of the peaks of valerenic and acetoxyvalerenic acids and the results for the reference standard are taken into account during calculations. The new test procedure is in line with the cross-cutting standardisation principle and can be recommended for inclusion into the draft monograph «Valerian tincture».

scholarly journals Relationships Between the Content of Phenolic Compounds and the Antioxidant Activity of Polish Honey Varieties as a Tool for Botanical Discrimination

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1810
Author(s):  
Monika Kędzierska-Matysek ◽  
Małgorzata Stryjecka ◽  
Anna Teter ◽  
Piotr Skałecki ◽  
Piotr Domaradzki ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Caffeic Acid ◽  
Principal Component ◽  
Total Content ◽  
Component Analysis ◽  
Total Phenolic ◽  
Hydroxybenzoic Acid ◽  
Coumaric Acid

The study compared the content of eight phenolic acids and four flavonoids and the antioxidant activity of six Polish varietal honeys. An attempt was also made to determine the correlations between the antioxidant parameters of the honeys and their polyphenol profile using principal component analysis. Total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity (ABTS) and reduction capacity (FRAP) were determined spectrophotometrically, and the phenolic compounds were determined using high-performance liquid chromatography (HPLC). The buckwheat honeys showed the strongest antioxidant activity, most likely because they had the highest concentrations of total phenols, total flavonoids, p-hydroxybenzoic acid, caffeic acid, p-coumaric acid, vanillic acid and chrysin. The principal component analysis (PCA) of the data showed significant relationships between the botanic origin of the honey, the total content of phenolic compounds and flavonoids and the antioxidant activity of the six Polish varietal honeys. The strongest, significant correlations were shown for parameters of antioxidant activity and TPC, TFC, p-hydroxybenzoic acid, caffeic acid and p-coumaric acid. Analysis of four principal components (explaining 86.9% of the total variance), as a classification tool, confirmed the distinctiveness of the Polish honeys in terms of their antioxidant activity and content of phenolic compounds.

Determination of Agarwood oil’s significant chemical compounds using principal component analysis

2020 ◽  
Author(s):  
Mohamad Hushnie Haron ◽  
Mohd Nasir Taib ◽  
Nurlaila Ismail ◽  
Nor Azah Mohd Ali ◽  
Saiful Nizam Tajuddin
Keyword(s):  
Principal Component Analysis ◽  
Principal Component ◽  
Chemical Compounds ◽  
Component Analysis ◽  
Significant Chemical

scholarly journals Determination of Flavonoids Compounds of Three Species and Different Harvesting Periods in Crataegi folium Based on LC-MS/MS

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1602
Author(s):  
Ya-Ping Guo ◽  
Hong Yang ◽  
Ya-Li Wang ◽  
Xiao-Xiang Chen ◽  
Ke Zhang ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Qualitative Analysis ◽  
Quantitative Method ◽  
Total Content ◽  
Qualitative And Quantitative Analysis ◽  
Pharmacological Activities ◽  
Time Saving ◽  
Qualitative And Quantitative ◽  
First Time

Crataegi folium have been used as medicinal and food materials worldwide due to its pharmacological activities. Although the leaves of Crataegus songorica (CS), Crataegus altaica (CA) and Crataegus kansuensis (CK) have rich resources in Xinjiang, China, they can not provide insights into edible and medicinal aspects. Few reports are available on the qualitative and quantitative analysis of flavonoids compounds of their leaves. Therefore, it is necessary to develop efficient methods to determine qualitative and quantitative flavonoids compounds in leaves of CS, CA and CK. In the study, 28 unique compounds were identified in CS versus CK by qualitative analysis. The validated quantitative method was employed to determine the content of eight flavonoids of the leaves of CS, CA and CK within 6 min. The total content of eight flavonoids was 7.8–15.1 mg/g, 0.1–9.1 mg/g and 4.8–10.7 mg/g in the leaves of CS, CA and CK respectively. Besides, the best harvesting periods of the three species were from 17th to 26th September for CS, from 30th September to 15th October for CA and CK. The validated and time-saving method was successfully implemented for the analysis of the content of eight flavonoids compounds in CS, CA and CK for the first time.

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