scholarly journals Oxidative Stability of Long-Chain Fatty Acids with Different Unsaturation Degrees into Layered Double Hydroxides

2021 ◽  
Vol 11 (15) ◽  
pp. 7035
Author(s):  
Francesca Blasi ◽  
Claudia Chiesi ◽  
Roberto Spogli ◽  
Lina Cossignani ◽  
Morena Nocchetti
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Oxidative Stability ◽  
Layered Double Hydroxides ◽  
Solid Phase ◽  
Essential Fatty Acids ◽  
Oxidative Modification ◽  
Anionic Form ◽  
Gas Chromatography Mass Spectrometry ◽  
Double Hydroxides

Nowadays, there is increasing evidence that the intake of essential fatty acids (FAs) and oleic acid has high nutritional importance. However, the vulnerability of these FAs to oxidation deserves special attention. FA oxidation may be avoided or delayed by intercalation of its anionic form in inorganic matrices as layered double hydroxides (LDH). Thus, the aim of the study was to evaluate the protective effects of MgAl LDH towards oleate (O), linoleate (L) and α-linolenate (Ln) degradation. The incorporation and the loading of different FAs in anionic form in LDH was determined by X-ray diffraction and thermogravimetric analysis (TGA), respectively. In order to study the selectivity of LDH towards the FA, the inorganic solid was equilibrated with a mixture of O, L and Ln (1:1:1). TGA and gas chromatography showed that Ln was preferentially intercalated. Free FA (FFA) and intercalated FA (IFA) were heated at 40 °C in the dark and then analyzed weekly for a maximum of 42 days. Their oxidative stability was evaluated by monitoring the primary and secondary oxidative compounds. The volatile compounds were determined by solid-phase microextraction, coupled with gas chromatography–mass spectrometry. Peroxide values were higher in FFA samples than in IFA samples, such as hexanal and trans,trans-2,4-heptadienal % contents. The results showed the potential of LDH intercalation for FA preservation from oxidative modification.

scholarly journals Volatile Organic Compound and Fatty Acid Profile of Milk from Cows and Buffaloes Fed Mycorrhizal or Nonmycorrhizal Ensiled Forage

Molecules ◽  
2019 ◽  
Vol 24 (8) ◽  
pp. 1616 ◽  
Author(s):  
Alessandro Genovese ◽  
Andrea Marrazzo ◽  
Lucia De Luca ◽  
Raffaele Romano ◽  
Nadia Manzo ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Fatty Acid ◽  
Mycorrhizal Fungi ◽  
Solid Phase ◽  
Saturated Fatty Acids ◽  
Gas Chromatography Mass Spectrometry ◽  
Volatile Fraction ◽  
Forage Crops ◽  
Seed Inoculation

Seed inoculation of forage crops by arbuscular mycorrhizal fungi (AMF) generally results in higher profitability, but also modifies the chemical composition of silage in terms of increased biomass, protein, and dry matter. Raw milk aroma is affected by the type of feed. This work investigated the influence of ensiled forage obtained by seed inoculation with AMF on the volatile fractions and fatty acid composition of milk. Two experiments were carried out: in the first, buffaloes were fed maize silage, and in the second, cows were fed sorghum silage. The volatile fractions of milk were quantified by headspace solid-phase micro-extraction (HS-SPME), combined with gas chromatography–mass spectrometry (GC-MS), and fatty acids by gas chromatography (GC). The ensiled forage obtained with AMF increased saturated fatty acids (SFAs), and decreased monounsaturated fatty acids (MUFAs) and polyunsaturated fatty acids (PUFAs) in both experiments. The volatile fraction in milk samples obtained from bovines fed mycorrhizal ensiled forage showed an increase of free fatty acids and ketones, responsible for cheesy and fruity odors. Aldehydes, responsible for green and grassy notes, increased only in the milk from buffaloes fed ensiled maize. Our results suggest that inoculation of maize and sorghum seed with AMF, combined with a low rate of fertilizers, leads to ensiled forage that could slightly affect the FA profile and odor quality of milk.

Methyl esters from the copepod Tisbe biminiensis assayed by two transesterification methods

Crustaceana ◽  
2013 ◽  
Vol 86 (11) ◽  
pp. 1343-1353 ◽  
Author(s):  
Lilian C. M. de Lima ◽  
Daniela M. A. F. Navarro ◽  
Lília P. Souza-Santos
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Acid Catalysis ◽  
Essential Fatty Acids ◽  
Methyl Esters ◽  
Saturated Fatty Acids ◽  
Fatty Acid Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Basic Catalysis ◽  
Transesterification Method

The present study assessed the efficiency of two transesterification methods for the identification of fatty acids in the copepodTisbe biminiensisVolkmann-Rocco, 1973. In the first method, the samples were esterified by basic catalysis with KOH in methanol, and in the second method they were esterified by acid catalysis with BF3. Fatty acids were identified by comparison of retention times and co-injection with standard Mix C4-C24, using gas chromatography and gas chromatography-mass spectrometry. Twenty-nine fatty acids were detected in samples transesterified by basic catalysis or acid catalysis. The predominant fatty acids were C18 (19.18%), C16 (13.02%), and C12 (11.39%) for samples transesterified by acid catalysis; and C13 (25.24%), C21 (11.32%), and C18 (10.65%) for samples transesterified by basic catalysis. The results show that fatty acid analysis for copepods may be affected by the transesterification method. Sample transesterification by acid catalysis was efficient to identify monosaturated and polyunsaturated fatty acids, whereas transesterification by basic catalysis was efficient to identify saturated fatty acids. Essential fatty acids were present in the copepod, which suggests thatT.biminiensisis a useful food to be used in larviculture.

scholarly journals PHYTOCHEMICAL ANALYSIS OF PORTULACA OLERACEA AND PORTULACA QUADRIFIDA EXTRACTS USING GAS CHROMATOGRAPHY–MASS SPECTROMETRY

2018 ◽  
Vol 11 (9) ◽  
pp. 204 ◽  
Author(s):  
Trupti P Durgawale ◽  
Chitra C Khanwelkar ◽  
Pratik P Durgawale
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Gas Chromatography ◽  
Antimicrobial Agents ◽  
Essential Fatty Acids ◽  
Portulaca Oleracea ◽  
Gas Chromatography Mass Spectrometry ◽  
Phytochemical Analysis ◽  
Ethanolic Extracts

Objective: The two plant species Portulaca oleracea and Portulaca quadrifida are commonly known as purslane and chickweed, respectively. They are typically consumed as salad or pickle. Traditional systems of medicine from Africa and China have described these plants belonging to family Portulacaceae as remedies against a host of diseases. Recent pharmacological investigations have revealed the importance of these plants as sources of antioxidants, essential fatty acids, and even antimicrobial agents. The objective of this study was phytochemical analysis and comparison of ethanolic extracts of these two species of Portulaca.Methods: The ethanolic extracts of both the species were prepared using Soxhlet extraction and were analyzed using gas chromatography coupled with mass spectrometry (GC–MS). Furthermore, the ethanolic extracts of fresh and dried whole plant of P. oleracea and seed of P. oleracea were studied.Results: The phytochemical constituents of ethanolic extracts of P. oleracea and P. quarifida were found to be quite different from one another and contained beneficial polyunsaturated fatty acids, alkaloids among other beneficial chemical species.Conclusion: The results of the study could be further used by researchers to assess the beneficial properties of both these species for in vitro and in vivo experiments.

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