scholarly journals Degradation of Brominated Organic Compounds (Flame Retardants) by a Four-Strain Consortium Isolated from Contaminated Groundwater

2021 ◽  
Vol 11 (14) ◽  
pp. 6263
Author(s):  
Noa Balaban ◽  
Faina Gelman ◽  
Alicia A. Taylor ◽  
Sharon L. Walker ◽  
Anat Bernstein ◽  
...  
Keyword(s):  
Flame Retardants ◽  
Environmental Enrichment ◽  
Microbial Consortium ◽  
Isotope Analysis ◽  
Industrial Area ◽  
Bacterial Consortium ◽  
Limiting Factor ◽  
Bacterial Consortia ◽  
Lab Experiments ◽  
Substrate Mixtures

Biodegradation of pollutants in the environment is directly affected by microbial communities and pollutant mixture at the site. Lab experiments using bacterial consortia and substrate mixtures are required to increase our understanding of these processes in the environment. One of the deficiencies of working with environmental cultures is the inability to culture and identify the active strains while knowing they are representative of the original environment. In the present study, we tested the aerobic microbial degradation of two brominated flame retardants, tribromo-neopentyl alcohol (TBNPA) and dibromo neopentyl glycol (DBNPG), by an assembled bacterial consortium of four strains. The four strains were isolated and plate-cultured from a consortium enriched from the impacted groundwater underlying the Neot Hovav industrial area (Negev, Israel), in which TBNPA and DBNPG are abundant pollutants. Total degradation (3–7 days) occurred only when the four-strain consortium was incubated together (25 °C; pH −7.2) with an additional carbon source, as both compounds were not utilized as such. Bacterial growth was found to be the limiting factor. A dual carbon–bromine isotope analysis was used to corroborate the claim that the isolated strains were responsible for the degradation in the original enriched consortium, thus ensuring that the isolated four-strain microbial consortium is representative of the actual environmental enrichment.

scholarly journals Efficient Biocontrol of Gaeumannomyces graminis var. Tritici in Wheat: Using Bacteria Isolated from Suppressive Soils

Agronomy ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2008
Author(s):  
Isabel Méndez ◽  
Ana Fallard ◽  
Isabel Soto ◽  
Gonzalo Tortella ◽  
María de la Luz Mora ◽  
...  
Keyword(s):  
Microbial Consortium ◽  
Bacterial Consortium ◽  
Chitinase Activity ◽  
Current Control ◽  
Control Measures ◽  
Gaeumannomyces Graminis ◽  
Biotic Factor ◽  
Bacterial Consortia ◽  
Gaeumannomyces Graminis Var Tritici ◽  
Take All

“Take-all” disease is the most important biotic factor affecting cereal productivity, causing 30–50% of crop losses. The causal agent is the ascomycete soil-borne pathogen Gaeumannomyces graminis var. tritici (Ggt). Current control measures are ineffective, because Ggt can remain saprophytic in soils for long periods. Therefore, the study of the microbiome residing in suppressive soils (SS) is a promising niche of Ggt biocontrol. Here, we evaluated the efficiency of Serratia sp., Bacillus sp., and Acinetobacter sp. isolated from SS against the incidence of Ggt on wheat. Our results demonstrated that plants inoculated with the bacterial consortium in both greenhouse and field conditions were highly efficient in Ggt biocontrol, more so than individual strains. The disease reduction was evidenced by higher biomass production, fewer copies of the Ggt genome with a concomitant curtailment of blackening of roots, a decrease of lipid peroxidation, and an increase of superoxide dismutase activity. The ability of the microbial consortium over that of single strains could be attributable to interspecies communication as a strategy to biocontrol; i.e., higher chitinase activity. In conclusion, bacterial consortia from SS are an important niche of Ggt biocontrol, serving as a model for other soil-borne pathogens.

Physiological Characterization of a Bacterial Consortium Reductively Dechlorinating 1,2,3- and 1,2,4-Trichlorobenzene

1998 ◽  
Vol 64 (2) ◽  
pp. 496-503 ◽  
Author(s):  
Lorenz Adrian ◽  
Werner Manz ◽  
Ulrich Szewzyk ◽  
Helmut Görisch
Keyword(s):  
Energy Source ◽  
Sulfate Reduction ◽  
Reductive Dechlorination ◽  
Microbial Consortium ◽  
Bacterial Consortium ◽  
Oligonucleotide Probes ◽  
Electron Sources ◽  
Physiological Characterization

ABSTRACT A bacterial mixed culture reductively dechlorinating trichlorobenzenes was established in a defined, synthetic mineral medium without any complex additions and with pyruvate as the carbon and energy source. The culture was maintained over 39 consecutive transfers of small inocula into fresh media, enriching the dechlorinating activity. In situ probing with fluorescence-labeled rRNA-targeted oligonucleotide probes revealed that two major subpopulations within the microbial consortium were phylogenetically affiliated with a sublineage within the Desulfovibrionaceaeand the gamma subclass of Proteobacteria. The bacterial consortium grew by fermentation of pyruvate, forming acetate, propionate, CO2, formate, and hydrogen. Acetate and propionate supported neither the reduction of trichlorobenzenes nor the reduction of sulfate when sulfate was present. Hydrogen and formate were used for sulfate reduction to sulfide. Sulfate strongly inhibited the reductive dechlorination of trichlorobenzenes. However, when sulfate was depleted in the medium due to sulfate reduction, dechlorination of trichlorobenzenes started. Similar results were obtained when sulfite was present in the cultures. Molybdate at a concentration of 1 mM strongly inhibited the dechlorination of trichlorobenzenes. Cultures supplied with molybdate plus sulfate did not reduce sulfate, but dechlorination of trichlorobenzenes occurred. Supplementation of electron-depleted cultures with various electron sources demonstrated that formate was used as a direct electron donor for reductive dechlorination, whereas hydrogen was not.

scholarly journals Raw Cow Milk Bacterial Consortium as Bioindicator of Circulating Anti-Microbial Resistance (AMR)

Animals ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2378
Author(s):  
Cristian Piras ◽  
Viviana Greco ◽  
Enrico Gugliandolo ◽  
Alessio Soggiu ◽  
Bruno Tilocca ◽  
...  
Keyword(s):  
Ecological Niche ◽  
Bacterial Species ◽  
Raw Milk ◽  
Bacterial Consortium ◽  
Cow Milk ◽  
Milk Analysis ◽  
Animal Products ◽  
Bacterial Consortia ◽  
Expression Of Genes ◽  
Ecological Pressure

The environment, including animals and animal products, is colonized by bacterial species that are typical and specific of every different ecological niche. Natural and human-related ecological pressure promotes the selection and expression of genes related to antimicrobial resistance (AMR). These genes might be present in a bacterial consortium but might not necessarily be expressed. Their expression could be induced by the presence of antimicrobial compounds that could originate from a given ecological niche or from human activity. In this work, we applied (meta)proteomics analysis of bacterial compartment of raw milk in order to obtain a method that provides a measurement of circulating AMR involved proteins and gathers information about the whole bacterial composition. Results from milk analysis revealed the presence of 29 proteins/proteoforms linked to AMR. The detection of mainly β-lactamases suggests the possibility of using the milk microbiome as a bioindicator for the investigation of AMR. Moreover, it was possible to achieve a culture-free qualitative and functional analysis of raw milk bacterial consortia.

scholarly journals The effect of heavy metals on thiocyanate biodegradation by an autotrophic microbial consortium enriched from mine tailings

2020 ◽  
Author(s):  
Farhad Shafiei ◽  
Mathew P. Watts ◽  
Lukas Pajank ◽  
John W. Moreau
Keyword(s):  
Heavy Metals ◽  
Mine Tailings ◽  
Energy Demand ◽  
High Efficiency ◽  
Microbial Consortium ◽  
Bacterial Consortium ◽  
Process Efficiency ◽  
Microbial Consortia ◽  
List Type ◽  
Gold Mine Tailings

AbstractBioremediation systems represent an environmentally sustainable approach to degrading industrially-generated thiocyanate (SCN-), with low energy demand and operational costs, and high efficiency and substrate specificity. However, heavy metals present in mine tailings effluent may hamper process efficiency by poisoning thiocyanate-degrading microbial consortia. Here we experimentally tested the tolerance of an autotrophic SCN--degrading bacterial consortium enriched from gold mine tailings for Zn, Cu, Ni, Cr, and As. All of the selected metals inhibited SCN- biodegradation to different extents, depending on concentration. At pH of 7.8 and 30°C, complete inhibition of SCN- biodegradation by Zn, Cu, Ni, and Cr occurred at 20, 5, 10, and 6 mg L-1, respectively. Lower concentrations of these metals decreased the rate of SCN- biodegradation, with relatively long lag times. Interestingly, the microbial consortium tolerated As even at 500 mg L-1, although both the rate and extent of SCN- biodegradation were affected. This study highlights the importance of considering metal co-contamination in bioreactor design and operation for SCN- bioremediation at mine sites.Key pointsBoth the efficiency and rate of SCN- biodegradation were inhibited by heavy metals, to different degrees depending on type and concentration of metalThe autotrophic microbial consortium was capable of tolerating high levels of As

scholarly journals BACTERIAL CONSORTIA FOR RETTING OF COCONUT HUSKS IN TANKS

CORD ◽  
1999 ◽  
Vol 15 (01) ◽  
pp. 34
Author(s):  
Anita Das Ravindranath ◽  
Saroi Bhosle
Keyword(s):  
Bacterial Consortium ◽  
Bacterial Consortia ◽  
Coconut Husk ◽  
Coir Fibre

A bacterial consortium developed on coconut husk leachates could ret coconut husk steeped for retting in rap water in a period of three months. The quality of the coir fibre obtained was comparable to traditionally retted fibre. The consortia could also bestow a greater degree of softness to the mechanically extracted coir fibre. Coir extraction can therefore be practiced by supplying the consortia for retting of husk for production of fibre and enhance the commercial utilization of the husk in coconut growing regions of Asia.

scholarly journals Establishing and Optimizing a Bacterial Consortia for Effective Biodegradation of Petroleum Contaminants: Advancing Classical Microbiology via Experimental and Mathematical Approach

Water ◽  
2021 ◽  
Vol 13 (22) ◽  
pp. 3311
Author(s):  
Baichun Wu ◽  
Jingmin Deng ◽  
Hao Niu ◽  
Jiahao Liang ◽  
Muhammad Arslan ◽  
...  
Keyword(s):  
Partial Correlation ◽  
High Efficiency ◽  
Bacterial Species ◽  
Rhodococcus Erythropolis ◽  
Bacterial Consortium ◽  
Bacterial Strains ◽  
Bacterial Consortia ◽  
Orthogonal Experiments ◽  
Petroleum Contaminants ◽  
Different Strains

In classical microbiology, developing a high-efficiency bacterial consortium is a great challenge for faster biodegradation of petroleum contaminants. In this study, a systematic experimental and mathematical procedure was adopted to establish a bacterial consortium for the effective biodegradation of heavy oil constituents. A total of 27 bacterial consortia were established as per orthogonal experiments, using 8 petroleum-degrading bacterial strains. These bacteria were closer phylogenetic relatives of Brevundimonas sp. Tibet-IX23 (Y1), Bacillus firmus YHSA15, B. cereus MTCC 9817, B. aquimaris AT8 (Y2, Y6 and Y7), Pseudomonas alcaligenes NBRC (Y3), Microbacterium oxydans CV8.4 (Y4), Rhodococcus erythropolis SBUG 2052 (Y5), and Planococcus sp. Tibet-IX21 (Y8), and were used in different combinations. Partial correlation analysis and a general linear model hereafter were applied to investigate interspecific relationships among different strains and consortia. The Y1 bacterial species showed a remarkable synergy, whereas Y3, Y4, and Y6 displayed a strong antagonism in all consortia. Inoculation ratios of different strains significantly influenced biodegradation. An optimal consortium was constructed with Y1, Y2, Y5, Y7, and Y8, which revealed maximum degradation of 11.238 mg/mL OD600 for oil contaminants. This study provides a line of evidence that a functional consortium can be established by mathematical models for improved bioremediation of petroleum-contaminated environment.

scholarly journals Simultaneous Bioremediation of Phenol and Cr (VI) from Tannery Wastewater Using Bacterial Consortium

2015 ◽  
Vol 3 (1) ◽  
pp. 50-55 ◽  
Author(s):  
Amrik Bhattacharya ◽  
Anshu Gupta ◽  
Amarjeet Kaur ◽  
Darshan Malik
Keyword(s):  
Bacterial Consortium ◽  
Agitation Speed ◽  
Tannery Wastewater ◽  
Tannery Effluent ◽  
Simultaneous Removal ◽  
Bacterial Strains ◽  
Static Mode ◽  
Simultaneous Reduction ◽  
Bacterial Consortia ◽  
Removal Of Contaminants

In the present study a consortium of four naturally isolated bacterial strains was evaluated as remediation tool for simultaneous removal ofphenol and Cr (VI) from tannery effluent. Application of bacterial consortia to effluent (pH 4.6) resulted in 100 and 78% removal of initial 47mg L-1 phenol and 16 mg L-1 Cr (VI), respectively at 96 h of treatment. The consortium was also active in removal of contaminants with lowerremoval rate in presence of extraneous higher concentrations of both phenol and Cr (VI). Treatment in static mode also resulted in removal ofpollutants, however with increase in agitation speed simultaneous reduction of contaminants becomes faster. Overall it can be inferred fromthe study that the above formulated bacterial consortium could effectively be used for treatment of phenol and Cr (VI) laden tannery and otherindustrial effluents.DOI: http://dx.doi.org/10.3126/ijasbt.v3i1.11889   Int J Appl Sci Biotechnol, Vol. 3(1): 50-55      

scholarly journals Dilution-to-Stimulation/Extinction Method: a Combination Enrichment Strategy To Develop a Minimal and Versatile Lignocellulolytic Bacterial Consortium

2020 ◽  
Vol 87 (2) ◽  
Author(s):  
Laura Díaz-García ◽  
Sixing Huang ◽  
Cathrin Spröer ◽  
Rocío Sierra-Ramírez ◽  
Boyke Bunk ◽  
...  
Keyword(s):  
Microbial Consortium ◽  
Plant Biomass ◽  
Bacterial Species ◽  
Bacterial Consortium ◽  
Priority Effects ◽  
Plant Residues ◽  
Agricultural Plant ◽  
Enrichment Strategy ◽  
Sequence Types ◽  
Bacterial Sequence

ABSTRACT The engineering of complex communities can be a successful path to understand the ecology of microbial systems and improve biotechnological processes. Here, we developed a strategy to assemble a minimal and effective lignocellulolytic microbial consortium (MELMC) using a sequential combination of dilution-to-stimulation and dilution-to-extinction approaches. The consortium was retrieved from Andean forest soil and selected through incubation in liquid medium with a mixture of three types of agricultural plant residues. After the dilution-to-stimulation phase, approximately 50 bacterial sequence types, mostly belonging to the Sphingobacteriaceae, Enterobacteriaceae, Pseudomonadaceae, and Paenibacillaceae, were significantly enriched. The dilution-to-extinction method demonstrated that only eight of the bacterial sequence types were necessary to maintain microbial growth and plant biomass consumption. After subsequent stabilization, only two bacterial species (Pseudomonas sp. and Paenibacillus sp.) became highly abundant (>99%) within the MELMC, indicating that these are the key players in degradation. Differences in the composition of bacterial communities between biological replicates indicated that selection, sampling, and/or priority effects could shape the consortium structure. The MELMC can degrade up to ∼13% of corn stover, consuming mostly its (hemi)cellulosic fraction. Tests with chromogenic substrates showed that the MELMC secretes an array of endoenzymes able to degrade xylan, arabinoxylan, carboxymethyl cellulose, and wheat straw. Additionally, the metagenomic profile inferred from the phylogenetic composition along with an analysis of carbohydrate-active enzymes of 20 bacterial genomes support the potential of the MELMC to deconstruct plant polysaccharides. This capacity was mainly attributed to the presence of Paenibacillus sp. IMPORTANCE The significance of our study mainly lies in the development of a combined top-down enrichment strategy (i.e., dilution to stimulation coupled to dilution to extinction) to build a minimal and versatile lignocellulolytic microbial consortium. We demonstrated that mainly two selectively enriched bacterial species (Pseudomonas sp. and Paenibacillus sp.) are required to drive the effective degradation of plant polymers. Our findings can guide the design of a synthetic bacterial consortium that could improve saccharification (i.e., the release of sugars from agricultural plant residues) processes in biorefineries. In addition, they can help to expand our ecological understanding of plant biomass degradation in enriched bacterial systems.

scholarly journals Engineered Interspecies Amino Acid Cross-Feeding Increases Population Evenness in a Synthetic Bacterial Consortium

mSystems ◽  
2019 ◽  
Vol 4 (4) ◽  
Author(s):  
Marika Ziesack ◽  
Travis Gibson ◽  
John K. W. Oliver ◽  
Andrew M. Shumaker ◽  
Bryan B. Hsu ◽  
...  
Keyword(s):  
Bacterial Species ◽  
Bacterial Consortium ◽  
Microbial Interactions ◽  
Environmental Benefits ◽  
Microbial Consortia ◽  
Positive Interactions ◽  
Complex Environments ◽  
Bacterial Consortia ◽  
Health And Disease ◽  
Antagonistic Interactions

ABSTRACT In nature, microbes interact antagonistically, neutrally, or beneficially. To shed light on the effects of positive interactions in microbial consortia, we introduced metabolic dependencies and metabolite overproduction into four bacterial species. While antagonistic interactions govern the wild-type consortium behavior, the genetic modifications alleviated antagonistic interactions and resulted in beneficial interactions. Engineered cross-feeding increased population evenness, a component of ecological diversity, in different environments, including in a more complex gnotobiotic mouse gut environment. Our findings suggest that metabolite cross-feeding could be used as a tool for intentionally shaping microbial consortia in complex environments. IMPORTANCE Microbial communities are ubiquitous in nature. Bacterial consortia live in and on our body and in our environment, and more recently, biotechnology is applying microbial consortia for bioproduction. As part of our body, bacterial consortia influence us in health and disease. Microbial consortium function is determined by its composition, which in turn is driven by the interactions between species. Further understanding of microbial interactions will help us in deciphering how consortia function in complex environments and may enable us to modify microbial consortia for health and environmental benefits.

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