scholarly journals Effects of Benzo[a]pyrene, Cortisol, and 17ß-Estradiol on Liver Microsomal EROD Activity of Anguilla anguilla: An In Vitro Approach

2021 ◽  
Vol 11 (6) ◽  
pp. 2533
Author(s):  
C.S.S. Ferreira ◽  
Miguel Oliveira ◽  
Maria Ana Santos ◽  
Mário Pacheco
Keyword(s):  
Steroid Hormones ◽  
Scientific Information ◽  
Endocrine System ◽  
Anguilla Anguilla ◽  
Erod Activity ◽  
Biotransformation Process ◽  
Hepatic Microsomes ◽  
Polycyclic Aromatic ◽  
In Vitro Effects

Fish liver ethoxyresorufin-O-deethylase (EROD) activity is widely used as biomarker of exposure to chemicals such as polycyclic aromatic hydrocarbons (PAHs). It is known that endocrine system plays a major role in fish stress mechanism. Despite the considerable scientific information about steroid hormone’s response, namely cortisol and 17ß-estradiol (E2), to stress situations, little is known about the influence of these hormones on enzymes involved on the biotransformation process. Thus, this study aimed to assess the in vitro effects of environmentally relevant concentrations of benzo[a]pyrene (B[a]P) (0.1, 0.3, 0.9, and 2.7 µM) and of two steroid hormones (cortisol and 17ß-estradiol) in a physiologically relevant concentration (5.997 ng/mL), alone or in combination, on Anguilla anguilla liver microsomal EROD activity, previously induced by 4 mg/kg β-naphthoflavone intraperitoneal injection. Hepatic microsomes in vitro exposure to the tested B[a]P concentrations induced a dose response inhibition of EROD activity, whereas exposure to cortisol significantly induced the activity of this enzyme. The steroid hormones were able to decrease the inhibitory effects of B[a]P on microsomal EROD activity, thus revealing a protective effect of these hormones over enzyme activity inhibited by contaminants.

scholarly journals Effect of Naringenin, Quercetin, and Sesamin on Xenobiotica-Metabolizing CYP1A and CYP3A in Mice Offspring after Maternal Exposure to Persistent Organic Pollutants

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Nadezhda Pilipenko ◽  
Erik Ropstad ◽  
Ruth Halsne ◽  
Galia Zamaratskaia
Keyword(s):  
Organic Pollutants ◽  
Persistent Organic Pollutants ◽  
Female Offspring ◽  
Erod Activity ◽  
Hepatic Microsomes ◽  
Dietary Phytochemicals ◽  
In Vitro Effects ◽  
Mice Liver ◽  
Different Levels

The aim of the present study was to evaluate in vitro effects of dietary phytochemicals naringenin, quercetin, and sesamin on the activities of ethoxy- (EROD; CYP1A) and benzyloxy- (BROD; CYP3A) resorufin O-dealkylases after the exposure to the cocktail of persistent organic pollutants (POPs). CD-1 mice were exposed from weaning, through gestation and lactation to a defined mixture of POPs. Hepatic microsomes were prepared from their female offspring at postnatal day 42. Hepatic EROD and BROD activity were evaluated in the presence of quercetin, naringenin, and sesamin at nine concentrations from 5 to 100000 nM. EROD activity was strongly inhibited by quercetin with Ki values from 1.7 to 2.6 μM. BROD activity was inhibited by quercetin with Ki values from 64.9 to 75.3 μM and naringenin with Ki values from 39.3 to 45.8 μM. The IC50 and Ki values did not differ between the groups of mice with different levels of POPs exposure in any of the experimental sets. Sesamin did not inhibit either EROD or BROD. We concluded that the interactions of quercetin and naringenin with CYP1A and CYP3A in mice liver were not affected by the levels of POPs exposure.

scholarly journals Protein Synthesis in Human Leukocytes and Lymphocytes: 1. Effect of Steroids and Sterols

Blood ◽  
1969 ◽  
Vol 34 (3) ◽  
pp. 348-356 ◽  
Author(s):  
SEYMOUR WERTHAMER ◽  
CARL HICKS ◽  
LEONARD AMARAL
Keyword(s):  
Protein Synthesis ◽  
Steroid Hormones ◽  
Plasma Protein ◽  
Human Lymphocytes ◽  
Amino Acid Incorporation ◽  
Binding Factor ◽  
In Vitro Effects ◽  
Physiologic Role

Abstract The in vitro effects of sterols, cholesterol and 3-methyl cholanthrene and steroids, cortisol, prednisolone and testosterone on protein synthesis in separate popultions of human lymphocytes and leukocytes has been investigated. It has been shown that all agents used result in the inhibition of protein synthesis under these conditions. It has also been shown that the inhibitory mechanism of the steroid hormones requires the presence of plasma, presumably as a protein binding factor in order to achieve its effect. The sterol, cholesterol and 3-methyl cholanthrene, in the absence of plasma, still inhibit amino acid incorporation. However, in the case of cholesterol, the magnitude of inhibition is lower than that observed in the presence of plasma, perhaps indicating a partial plasma dependence. The results presented therefore support the hypothesis that the inhibition of lymphocyte protein synthesis by steroid hormones occurs only when the steroid is bound to a plasma protein. The physiologic role of the plasma protein-cortisol complex and its relation to the condition of lymphopenia in man is discussed.

scholarly journals In Vitro Effects of Steroid Hormones on Frog Melanophores

1971 ◽  
Vol 57 (5) ◽  
pp. 337-342 ◽  
Author(s):  
Peggy J Himes ◽  
Mac E Hadley
Keyword(s):  
Steroid Hormones ◽  
In Vitro Effects

In vitro effects of different steroid hormones on superoxide anion production of human neutrophil granulocytes

Steroids ◽  
2000 ◽  
Vol 65 (12) ◽  
pp. 889-894 ◽  
Author(s):  
Gábor Békési ◽  
Réka Kakucs ◽  
Szabolcs Várbı́ró ◽  
Károly Rácz ◽  
Detlef Sprintz ◽  
...  
Keyword(s):  
Steroid Hormones ◽  
Human Neutrophil ◽  
Superoxide Anion ◽  
Neutrophil Granulocytes ◽  
Superoxide Anion Production ◽  
Anion Production ◽  
In Vitro Effects

scholarly journals Local Release of Steroid Hormones, Prostaglandin E2, and Endothelin-1 from Bovine Mature Follicles In Vitro: Effects of Luteinizing Hormone, Endothelin-1, and Cytokines

1998 ◽  
Vol 59 (2) ◽  
pp. 437-443 ◽  
Author(s):  
Tomas J. Acosta ◽  
Akio Miyamoto ◽  
Takahiro Ozawa ◽  
Missaka P.B. Wijayagunawardane ◽  
Kunitada Sato
Keyword(s):  
Luteinizing Hormone ◽  
Prostaglandin E2 ◽  
Steroid Hormones ◽  
Endothelin 1 ◽  
In Vitro Effects ◽  
Local Release
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