scholarly journals Effect of Naringenin, Quercetin, and Sesamin on Xenobiotica-Metabolizing CYP1A and CYP3A in Mice Offspring after Maternal Exposure to Persistent Organic Pollutants

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Nadezhda Pilipenko ◽  
Erik Ropstad ◽  
Ruth Halsne ◽  
Galia Zamaratskaia
Keyword(s):  
Organic Pollutants ◽  
Persistent Organic Pollutants ◽  
Female Offspring ◽  
Erod Activity ◽  
Hepatic Microsomes ◽  
Dietary Phytochemicals ◽  
In Vitro Effects ◽  
Mice Liver ◽  
Different Levels

The aim of the present study was to evaluate in vitro effects of dietary phytochemicals naringenin, quercetin, and sesamin on the activities of ethoxy- (EROD; CYP1A) and benzyloxy- (BROD; CYP3A) resorufin O-dealkylases after the exposure to the cocktail of persistent organic pollutants (POPs). CD-1 mice were exposed from weaning, through gestation and lactation to a defined mixture of POPs. Hepatic microsomes were prepared from their female offspring at postnatal day 42. Hepatic EROD and BROD activity were evaluated in the presence of quercetin, naringenin, and sesamin at nine concentrations from 5 to 100000 nM. EROD activity was strongly inhibited by quercetin with Ki values from 1.7 to 2.6 μM. BROD activity was inhibited by quercetin with Ki values from 64.9 to 75.3 μM and naringenin with Ki values from 39.3 to 45.8 μM. The IC50 and Ki values did not differ between the groups of mice with different levels of POPs exposure in any of the experimental sets. Sesamin did not inhibit either EROD or BROD. We concluded that the interactions of quercetin and naringenin with CYP1A and CYP3A in mice liver were not affected by the levels of POPs exposure.

scholarly journals Effects of Benzo[a]pyrene, Cortisol, and 17ß-Estradiol on Liver Microsomal EROD Activity of Anguilla anguilla: An In Vitro Approach

2021 ◽  
Vol 11 (6) ◽  
pp. 2533
Author(s):  
C.S.S. Ferreira ◽  
Miguel Oliveira ◽  
Maria Ana Santos ◽  
Mário Pacheco
Keyword(s):  
Steroid Hormones ◽  
Scientific Information ◽  
Endocrine System ◽  
Anguilla Anguilla ◽  
Erod Activity ◽  
Biotransformation Process ◽  
Hepatic Microsomes ◽  
Polycyclic Aromatic ◽  
In Vitro Effects

Fish liver ethoxyresorufin-O-deethylase (EROD) activity is widely used as biomarker of exposure to chemicals such as polycyclic aromatic hydrocarbons (PAHs). It is known that endocrine system plays a major role in fish stress mechanism. Despite the considerable scientific information about steroid hormone’s response, namely cortisol and 17ß-estradiol (E2), to stress situations, little is known about the influence of these hormones on enzymes involved on the biotransformation process. Thus, this study aimed to assess the in vitro effects of environmentally relevant concentrations of benzo[a]pyrene (B[a]P) (0.1, 0.3, 0.9, and 2.7 µM) and of two steroid hormones (cortisol and 17ß-estradiol) in a physiologically relevant concentration (5.997 ng/mL), alone or in combination, on Anguilla anguilla liver microsomal EROD activity, previously induced by 4 mg/kg β-naphthoflavone intraperitoneal injection. Hepatic microsomes in vitro exposure to the tested B[a]P concentrations induced a dose response inhibition of EROD activity, whereas exposure to cortisol significantly induced the activity of this enzyme. The steroid hormones were able to decrease the inhibitory effects of B[a]P on microsomal EROD activity, thus revealing a protective effect of these hormones over enzyme activity inhibited by contaminants.

scholarly journals A Human Relevant Defined Mixture of Persistent Organic Pollutants (POPs) Affects In Vitro Secretion of Glucagon-Like Peptide 1 (GLP-1), but Does Not Affect Translocation of Its Receptor

2019 ◽  
Vol 172 (2) ◽  
pp. 359-367 ◽  
Author(s):  
Maeve Shannon ◽  
Yuling Xie ◽  
Steven Verhaegen ◽  
Jodie Wilson ◽  
Hanne F Berntsen ◽  
...  
Keyword(s):  
Organic Pollutants ◽  
Persistent Organic Pollutants ◽  
Complex Mixture ◽  
Blood Levels ◽  
Environmental Toxicants ◽  
Blood Concentrations ◽  
Total Mixture ◽  
Glucagon Like Peptide ◽  
Glucagon Like Peptide 1

Abstract Environmental exposure to persistent organic pollutants (POPs) has been suggested as a contributing factor for the increased rate of type 2 diabetes and obesity. A complex mixture of 29 POPs (Total mixture), based on human blood concentrations, was used to expose a glucagon-like peptide 1 (GLP-1) secreting enteroendocrine cell line (pGIP/neo: STC-1) in vitro for 3 and 24 h. Significant increases of GLP-1 occurred when cells were exposed to the Total mixture at ×500 blood levels. Six sub-mixtures representing chlorinated (Cl), brominated (Br), and perfluorinated chemicals (PFAA), and their combinations (Cl + Br, Cl + PFAA, Br + PFAA) were also tested at ×500. Secretion levels seen for these remained lower than the Total mixture, and the Br mixture had no effect. After 24 h, increased secretion was seen with all mixtures at ×1 blood levels. Cytotoxicity was present for ×100 and ×500 blood levels. When tested in a GLP-1 receptor translocation assay (U2OS-GLP1R-EGFP), neither agonistic nor antagonist effects on receptor internalization were seen for any of the mixtures. We conclude individual classes of POPs, alone or in combination, can affect GLP-1 secretion and may contribute as a molecular mechanism linking environmental toxicants and diabetes.

scholarly journals Effects of a human-based mixture of persistent organic pollutants on the in vivo exposed cerebellum and cerebellar neuronal cultures exposed in vitro

2021 ◽  
Vol 146 ◽  
pp. 106240
Author(s):  
Hanne Friis Berntsen ◽  
Nur Duale ◽  
Cesilie Granum Bjørklund ◽  
Oscar Daniel Rangel-Huerta ◽  
Kine Dyrberg ◽  
...  
Keyword(s):  
Organic Pollutants ◽  
Persistent Organic Pollutants ◽  
Neuronal Cultures

scholarly journals Hydrophobized Reversed-Phase Adsorbent for Protection of Dairy Cattle against Lipophilic Toxins from Diet. Efficiensy In Vitro and In Vivo

Toxins ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 256
Author(s):  
Alexander Sotnichenko ◽  
Evgeny Pantsov ◽  
Dmitry Shinkarev ◽  
Victor Okhanov
Keyword(s):  
Dairy Cattle ◽  
Organic Pollutants ◽  
Persistent Organic Pollutants ◽  
Polyaromatic Hydrocarbons ◽  
Reversed Phase ◽  
Chemical Pollution ◽  
Lipophilic Toxins ◽  
Cattle Feeds

The steady growth of inflammatory diseases of the udder in dairy cattle forces us to look for the causes of this phenomenon in the context of growing chemical pollution of the environment and feeds. Within the framework of this concept, an analysis was made of the polarity level of the three toxic impurity groups, which are commonly present in dairy cattle feeds. These impurities are presented by mycotoxins, polyaromatic hydrocarbons (PAH) and persistent organic pollutants (POP). It has been determined that 46% of studied mycotoxins (n = 1500) and 100% of studied polyaromatic hydrocarbons (n = 45) and persistent organic pollutants (n = 55) are lipophilic compounds, prone to bioaccumulation. A comparative evaluation of the sorption capacity of four adsorbents of a different nature and polarity with respect to the simplest PAH, naphthalene and lipophilic estrogenic mycotoxin, zearalenone in vitro has been carried out. The highest efficiency in these experiments was demonstrated by the reversed-phase polyoctylated polysilicate hydrogel (POPSH). The use of POPSH in a herd of lactating cows significantly reduced the transfer of aldrin, dieldrin and heptachlor, typical POPs from the “dirty dozen”, to the milk. The relevance of protecting the main functional systems of animals from the damaging effects of lipophilic toxins from feeds using non-polar adsorbents, and the concept of evaluating the effectiveness of various feed adsorbents for dairy cattle by their influence on the somatic cell count in the collected milk are discussed.

scholarly journals Profile occurrences and in vitro effects of toxic organic pollutants in metal shredding facilities in Wallonia (Belgium)

2021 ◽  
pp. 127009
Author(s):  
Thi-Que Doan ◽  
Anh-Duc Pham ◽  
Jean-Marc Brouhon ◽  
Johan Lundqvist ◽  
Marie-Louise Scippo
Keyword(s):  
Organic Pollutants ◽  
In Vitro Effects

In vitro assessment of environmental stress of persistent organic pollutants on the Indo-Pacific humpback dolphin

2015 ◽  
Vol 30 (1) ◽  
pp. 529-535 ◽  
Author(s):  
Kuntong Jia ◽  
Liang Ding ◽  
Lingli Zhang ◽  
Mei Zhang ◽  
Meisheng Yi ◽  
...  
Keyword(s):  
Environmental Stress ◽  
Organic Pollutants ◽  
Persistent Organic Pollutants ◽  
In Vitro Assessment

scholarly journals Persistent organic pollutants have dose and CAG repeat length dependent effects on androgen receptor activity in vitro

2011 ◽  
Vol 32 (3) ◽  
pp. 293-297 ◽  
Author(s):  
Christel Björk ◽  
Hannah Nenonen ◽  
Aleksander Giwercman ◽  
Åke Bergman ◽  
Lars Rylander ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Organic Pollutants ◽  
Persistent Organic Pollutants ◽  
Repeat Length ◽  
Cag Repeat ◽  
Receptor Activity ◽  
Androgen Receptor Activity
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