scholarly journals Non-Invasive Measurement, Mathematical Simulation and In Situ Detection of Biofilm Evolution in Porous Media: A Review

2021 ◽  
Vol 11 (4) ◽  
pp. 1391
Author(s):  
Yajun Zhang ◽  
Aoshu Xu ◽  
Xin Lv ◽  
Qian Wang ◽  
Caihui Feng ◽  
...  
Keyword(s):  
Porous Media ◽  
Mathematical Simulation ◽  
Pore Space ◽  
Three Dimensional ◽  
Structural Complexity ◽  
In Situ Monitoring ◽  
Spatiotemporal Resolution ◽  
Non Invasive ◽  
Invasive Measurement

The development of biofilms and the related changes in porous media in the subsurface cannot be directly observed and evaluated. The primary reason that the mechanism of biofilm clogging in porous media cannot be clearly demonstrated is due to the opacity and structural complexity of three-dimensional pore space. Interest in exploring methods to overcome this limitation has been increasing. In the first part of this review, we introduce the underlying characteristics of biofilm in porous media. Then, we summarize two approaches, non-invasive measurement methods and mathematical simulation strategies, for studying fluid–biofilm–porous medium interaction with spatiotemporal resolution. We also discuss the advantages and limitations of these approaches. Lastly, we provide a perspective on opportunities for in situ monitoring at the field site.

scholarly journals A microscopic approach to investigate bacteria under in situ conditions in sea-ice samples

2001 ◽  
Vol 33 ◽  
pp. 304-310 ◽  
Author(s):  
Karen Junge ◽  
Christopher Krembs ◽  
Jody Deming ◽  
Aaron Stierle ◽  
Hajo Eicken
Keyword(s):  
Sea Ice ◽  
Pore Space ◽  
Three Dimensional ◽  
Epifluorescence Microscopy ◽  
Microbial Populations ◽  
Microbial Life ◽  
Dimensional Network ◽  
In Situ Conditions ◽  
Fluorescent Stain

AbstractMicrobial populations and activity within sea ice have been well described based on bulk measurements from melted sea-ice samples. However, melting destroys the micro-environments within the ice matrix and does not allow for examination of microbial populations at a spatial scale relevant to the organism. Here, we describe the development of a new method allowing for microscopic observations of bacteria localized within the three-dimensional network of brine inclusions in sea ice under in situ conditions. Conventional bacterial staining procedures, using the DNA-specific fluorescent stain DAPI, epifluorescence microscopy and image analysis, were adapted to examine bacteria and their associations with various surfaces within microtomed sections of sea ice at temperatures from −2° to −15°C. The utility and sensitivity of the method were demonstrated by analyzing artificial sea-ice preparations of decimal dilutions of a known bacterial culture. When applied to natural, particle-rich sea ice, the method allowed distinction between bacteria and particles at high magnification. At lower magnifications, observations of bacteria could be combined with those of other organisms and with morphology and particle content of the pore space. The method described here may ultimately aid in discerning constraints on microbial life at extremely low temperatures.

scholarly journals Network of Palladium-Based Nanorings Synthesized by Liquid-Phase Reduction Using DMSO-H2O: In Situ Monitoring of Structure Formation and Drying Deformation by ASEM

2020 ◽  
Vol 21 (9) ◽  
pp. 3271 ◽  
Author(s):  
Takuki Komenami ◽  
Akihiro Yoshimura ◽  
Yasunari Matsuno ◽  
Mari Sato ◽  
Chikara Sato
Keyword(s):  
Surface Tension ◽  
Liquid Phase ◽  
Aqueous Solutions ◽  
Critical Point ◽  
Synthesis Method ◽  
Three Dimensional ◽  
In Situ Monitoring ◽  
Critical Point Drying ◽  
Micrometer Size

We developed a liquid-phase synthesis method for Pd-based nanostructure, in which Pd dissolved in dimethyl sulfoxide (DMSO) solutions was precipitated using acid aqueous solution. In the development of the method, in situ monitoring using atmospheric scanning electron microscopy (ASEM) revealed that three-dimensional (3D) Pd-based nanonetworks were deformed to micrometer-size particles possibly by the surface tension of the solutions during the drying process. To avoid surface tension, critical point drying was employed to dry the Pd-based precipitates. By combining ASEM monitoring with critical point drying, the synthesis parameters were optimized, resulting in the formation of lacelike delicate nanonetworks using citric acid aqueous solutions. Precipitation using HCl acid aqueous solutions allowed formation of 500-nm diameter nanorings connected by nanowires. The 3D nanostructure formation was controllable and modifiable into various shapes using different concentrations of the Pd and Cl ions as the parameters.

In situ monitoring of the seed stage of a fermentation process using non-invasive NIR spectrometry

The Analyst ◽  
2008 ◽  
Vol 133 (5) ◽  
pp. 660 ◽  
Author(s):  
Alison Nordon ◽  
David Littlejohn ◽  
Alison S. Dann ◽  
Paul A. Jeffkins ◽  
Mark D. Richardson ◽  
...  
Keyword(s):  
Fermentation Process ◽  
In Situ Monitoring ◽  
Non Invasive

Regeneration of testis tissue after ectopic implantation of porcine testis cell aggregates in mice: improved consistency of outcomes and in situ monitoring

2020 ◽  
Vol 32 (6) ◽  
pp. 594 ◽  
Author(s):  
Awang Hazmi Awang-Junaidi ◽  
Jaswant Singh ◽  
Ali Honaramooz
Keyword(s):  
De Novo ◽  
Ultrasound Biomicroscopy ◽  
In Situ Monitoring ◽  
Cell Aggregates ◽  
Tissue Development ◽  
Immunodeficient Mice ◽  
Non Invasive ◽  
Old Donor ◽  
Testis Tissue

Ectopic implantation of donor testis cell aggregates in recipient mice results in de novo formation or regeneration of testis tissue and, as such, provides a unique invivo model for the study of testis development. However, currently the results are inconsistent and the efficiency of the model remains low. This study was designed to: (1) examine several factors that can potentially improve the consistency and efficiency of this model and (2) explore the use of ultrasound biomicroscopy (UBM) for the non-invasive invivo evaluation of implants. Testis cell aggregates, containing ~40% gonocytes, from 1-week-old donor piglets were implanted under the back skin of immunodeficient mice through skin incisions using gel matrices or through subcutaneous injection without using gel matrices. The addition of gel matrices led to inconsistent tissue development; gelatin had the greatest development, followed by collagen, whereas agarose resulted in poor development. The results also depended on the implanted cell numbers since implants with 100×106 cells were larger than those with 50×106 cells. The injection approach for cell implantation was less invasive and resulted in more consistent and efficient testis tissue development. UBM provided promising results as a means of non-invasive monitoring of implants.

scholarly journals Clinical system for non-invasive in situ monitoring of gases in the human paranasal sinuses

2009 ◽  
Vol 17 (13) ◽  
pp. 10849 ◽  
Author(s):  
Märta Lewander ◽  
Zuguang Guan ◽  
Katarina Svanberg ◽  
Sune Svanberg ◽  
Tomas Svensson
Keyword(s):  
Paranasal Sinuses ◽  
In Situ Monitoring ◽  
Non Invasive

Immiscible Displacement at Pore Level for Correlated Porous Media

1998 ◽  
Vol 09 (06) ◽  
pp. 837-849 ◽  
Author(s):  
A. M. Vidales ◽  
J. L. Riccardo ◽  
G. Zgrablich
Keyword(s):  
Porous Media ◽  
Pore Space ◽  
Three Dimensional ◽  
Immiscible Displacement ◽  
Porous Network ◽  
Buoyancy Forces ◽  
Correlation Strength ◽  
Pore Level

Immiscible displacement at pore level on a three-dimensional correlated porous network is simulated allowing flow of the wetting phase along crevices of the pore walls (possibility of snap-off in throats) and advance through the centers of the pore space with different pore and throat filling conditions, leading to a cooperative filling. When these two mechanisms compete, different patterns arise. We study the effect of the correlation strength on the onset of each pattern. We do not take buoyancy forces into account.

3D Simulation of Tunnel Collapse Consolidation and In Situ Monitoring Analysis

2011 ◽  
Vol 243-249 ◽  
pp. 3551-3555
Author(s):  
Zhi Min Chen ◽  
De An Zhao
Keyword(s):  
Numerical Models ◽  
Three Dimensional ◽  
In Situ Monitoring ◽  
3D Simulation ◽  
Actual Situation ◽  
Consolidation Effect ◽  
Tunnel Collapse ◽  
Simulation Results ◽  
Shallow Buried Tunnel

A shallow buried tunnel was collapsed during construction. According to the actual situation of the tunnel, different collapse consolidation schemes were put forward and corresponding three-dimensional finite difference numerical models were established and analyzed using FLAC3D software. Base on the 3D Simulation results, a collapse consolidation scheme was determined and constructed. The in-situ monitoring results of vault subsidence and convergence displacement were consistent approximately with the 3D Simulation results. The in-situ measured results were in line with the specification’s allowance and show that the consolidation effect of reinforced concrete casing arch and ahead grouting method is good. This could be referenced for similar engineering.

scholarly journals Pore-scale investigation of the displacement fluid mechanics during two-phase flows in natural porous media under the dominance of capillary forces

Georesursy ◽  
2020 ◽  
Vol 22 (1) ◽  
pp. 4-12
Author(s):  
Timur R. Zakirov ◽  
Maxim G. Khramchenkov
Keyword(s):  
Porous Media ◽  
Pore Space ◽  
Three Dimensional ◽  
Capillary Forces ◽  
High Temporal Resolution ◽  
Pore Scale ◽  
Two Phase Flows ◽  
Two Phase ◽  
Pressure Drops ◽  
Mobile Interfaces

This paper presents the results of numerical simulations of two-phase flows in porous media under capillary forces dominance. For modeling of immiscible two-phase flow, the lattice Boltzmann equations with multi relaxation time operator were applied, and the interface phenomena was described with the color-gradient method. The objective of study is to establish direct links between quantitative characteristics of the flow and invasion events, using high temporal resolution when detecting simulation results. This is one of the few works where Haines jumps (rapid invasion event which occurs at meniscus displacing from narrow pore throat to its wide body) are considered in three-dimensional natural pore space, but the focus is also on the displacement mechanics after jumps. It was revealed the sequence of pore scale events which can be considered as a period of drainage process: rapid invasion event during Haines jump; finish of jump and continuation of uniform invasion in current pore; switching of mobile interfaces and displacement in new region. The detected interface change, along with Haines jump, is another distinctive feature of the capillary forces action. The change of the mobile interfaces is manifested in step-like behavior of the front movement. It was obtained that statistical distributions of pressure drops during Haines jumps obey lognormal law. When investigating the flow rate and surface tension effect on the pressure drop statistics it was revealed that these parameters practically don’t affect on the statistical distribution and influence only on the magnitude of pressure drops and the number of individual Haines jumps.

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