Regeneration of testis tissue after ectopic implantation of porcine testis cell aggregates in mice: improved consistency of outcomes and in situ monitoring

2020 ◽  
Vol 32 (6) ◽  
pp. 594 ◽  
Author(s):  
Awang Hazmi Awang-Junaidi ◽  
Jaswant Singh ◽  
Ali Honaramooz
Keyword(s):  
De Novo ◽  
Ultrasound Biomicroscopy ◽  
In Situ Monitoring ◽  
Cell Aggregates ◽  
Tissue Development ◽  
Immunodeficient Mice ◽  
Non Invasive ◽  
Old Donor ◽  
Testis Tissue

Ectopic implantation of donor testis cell aggregates in recipient mice results in de novo formation or regeneration of testis tissue and, as such, provides a unique invivo model for the study of testis development. However, currently the results are inconsistent and the efficiency of the model remains low. This study was designed to: (1) examine several factors that can potentially improve the consistency and efficiency of this model and (2) explore the use of ultrasound biomicroscopy (UBM) for the non-invasive invivo evaluation of implants. Testis cell aggregates, containing ~40% gonocytes, from 1-week-old donor piglets were implanted under the back skin of immunodeficient mice through skin incisions using gel matrices or through subcutaneous injection without using gel matrices. The addition of gel matrices led to inconsistent tissue development; gelatin had the greatest development, followed by collagen, whereas agarose resulted in poor development. The results also depended on the implanted cell numbers since implants with 100×106 cells were larger than those with 50×106 cells. The injection approach for cell implantation was less invasive and resulted in more consistent and efficient testis tissue development. UBM provided promising results as a means of non-invasive monitoring of implants.

In situ monitoring of the seed stage of a fermentation process using non-invasive NIR spectrometry

The Analyst ◽  
2008 ◽  
Vol 133 (5) ◽  
pp. 660 ◽  
Author(s):  
Alison Nordon ◽  
David Littlejohn ◽  
Alison S. Dann ◽  
Paul A. Jeffkins ◽  
Mark D. Richardson ◽  
...  
Keyword(s):  
Fermentation Process ◽  
In Situ Monitoring ◽  
Non Invasive

scholarly journals Clinical system for non-invasive in situ monitoring of gases in the human paranasal sinuses

2009 ◽  
Vol 17 (13) ◽  
pp. 10849 ◽  
Author(s):  
Märta Lewander ◽  
Zuguang Guan ◽  
Katarina Svanberg ◽  
Sune Svanberg ◽  
Tomas Svensson
Keyword(s):  
Paranasal Sinuses ◽  
In Situ Monitoring ◽  
Non Invasive

scholarly journals Non-Invasive Measurement, Mathematical Simulation and In Situ Detection of Biofilm Evolution in Porous Media: A Review

2021 ◽  
Vol 11 (4) ◽  
pp. 1391
Author(s):  
Yajun Zhang ◽  
Aoshu Xu ◽  
Xin Lv ◽  
Qian Wang ◽  
Caihui Feng ◽  
...  
Keyword(s):  
Porous Media ◽  
Mathematical Simulation ◽  
Pore Space ◽  
Three Dimensional ◽  
Structural Complexity ◽  
In Situ Monitoring ◽  
Spatiotemporal Resolution ◽  
Non Invasive ◽  
Invasive Measurement

The development of biofilms and the related changes in porous media in the subsurface cannot be directly observed and evaluated. The primary reason that the mechanism of biofilm clogging in porous media cannot be clearly demonstrated is due to the opacity and structural complexity of three-dimensional pore space. Interest in exploring methods to overcome this limitation has been increasing. In the first part of this review, we introduce the underlying characteristics of biofilm in porous media. Then, we summarize two approaches, non-invasive measurement methods and mathematical simulation strategies, for studying fluid–biofilm–porous medium interaction with spatiotemporal resolution. We also discuss the advantages and limitations of these approaches. Lastly, we provide a perspective on opportunities for in situ monitoring at the field site.

Soft contact lens biosensor for in situ monitoring of tear glucose as non-invasive blood sugar assessment

Talanta ◽  
2011 ◽  
Vol 83 (3) ◽  
pp. 960-965 ◽  
Author(s):  
Ming Xing Chu ◽  
Kumiko Miyajima ◽  
Daishi Takahashi ◽  
Takahiro Arakawa ◽  
Kenji Sano ◽  
...  
Keyword(s):  
Blood Sugar ◽  
Contact Lens ◽  
In Situ Monitoring ◽  
Soft Contact ◽  
Non Invasive ◽  
Soft Contact Lens ◽  
Tear Glucose

scholarly journals In situ monitoring of powder blending by non-invasive Raman spectrometry with wide area illumination

2013 ◽  
Vol 76 ◽  
pp. 28-35 ◽  
Author(s):  
Pamela Allan ◽  
Luke J. Bellamy ◽  
Alison Nordon ◽  
David Littlejohn ◽  
John Andrews ◽  
...  
Keyword(s):  
In Situ Monitoring ◽  
Wide Area ◽  
Raman Spectrometry ◽  
Non Invasive ◽  
Powder Blending

scholarly journals A de novo strategy to develop NIR precipitating fluorochrome for long-term in situ cell membrane bioimaging

2021 ◽  
Vol 118 (8) ◽  
pp. e2018033118
Author(s):  
Ke Li ◽  
Yifan Lyu ◽  
Yan Huang ◽  
Shuai Xu ◽  
Hong-Wen Liu ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Fluorescent Probes ◽  
Near Infrared ◽  
De Novo ◽  
Membrane Surface ◽  
In Situ Monitoring ◽  
Fluorescence Signal ◽  
In Situ Imaging

Cell membrane–targeted bioimaging is a prerequisite for studying the roles of membrane-associated biomolecules in various physiological and pathological processes. However, long-term in situ bioimaging on the cell membrane with conventional fluorescent probes leads to diffusion into cells from the membrane surface. Therefore, we herein proposed a de novo strategy to construct an antidiffusion probe by integrating a fluorochrome characterized by strong hydrophobicity and low lipophilicity, with an enzyme substrate to meet this challenge. This precipitating fluorochrome HYPQ was designed by conjugating the traditionally strong hydrophobic solid-state fluorochrome 6-chloro–2-(2-hydroxyphenyl) quinazolin-4(3H)-one (HPQ) with a 2-(2-methyl–4H-chromen–4-ylidene) malononitrile group to obtain closer stacking to lower lipophilicity and elongate emission to the far-red to near-infrared wavelength. As proof-of-concept, the membrane-associated enzyme γ-glutamyltranspeptidase (GGT) was selected as a model enzyme to design the antidiffusion probe HYPQG. Then, benefiting from the precipitating and stable signal properties of HYPQ, in situ imaging of GGT on the membrane was successfully realized. Moreover, after HYPQG was activated by GGT, the fluorescence signal on the cell membrane remained unchanged, with incubation time even extending to 6 h, which is significant for in situ monitoring of enzymatic activity. In vivo testing subsequently showed that the tumor region could be accurately defined by this probe after long-term in situ imaging of tumor-bearing mice. The excellent performance of HYPQ indicates that it may be an ideal alternative for constructing universal antidiffusion fluorescent probes, potentially providing an efficient tool for accurate imaging-guided surgery in the future.

scholarly journals 268 GERM CELL DEVELOPMENT IN EQUINE TESTIS TISSUE XENOGRAFTED INTO MICE

2005 ◽  
Vol 17 (2) ◽  
pp. 283 ◽  
Author(s):  
R. Turner ◽  
R. Rathi ◽  
A. Honaramooz ◽  
W. Zeng ◽  
I. Dobrinski
Keyword(s):  
Chorionic Gonadotropin ◽  
Germ Cells ◽  
Seminal Vesicles ◽  
Post Transplantation ◽  
Immunodeficient Mice ◽  
Donor Tissue ◽  
Old Donor ◽  
Pachytene Spermatocytes ◽  
Donor Species ◽  
Testis Tissue

Grafting of testis tissue from immature animals under the back skin of immunodeficient mice results in complete spermatogenesis, albeit with different levels of efficiency in different species. While spermatogenesis develops comparably to that in the donor species in xenografts from pigs, sheep and goats, spermatogenic differentiation is less efficient in testis tissue from cats and bulls. Testicular maturation was significantly accelerated in rhesus monkey testis grafts whereas timing was similar to that in the donor species in cats and bulls. The objective of this study was to investigate if grafting of immature horse testis tissue would result in spermatogenesis in a mouse host. Small fragments of testis tissue (about 1 mm3) from four sexually immature colts (2-week-old Standardbred, 5- and 8-month-old ponies, 10-month-old Warmblood) were grafted under the back skin of castrated male immunodeficient mice (n = 5, 5, 10 and 5 recipient mice, respectively). Histological examination of the testis xenografts was performed between 14 and 50 week post-transplantation. Weight of the seminal vesicles in the host mouse was recorded as an indicator of bioactive testosterone produced by the xenografts. At the time of grafting, the seminiferous cords of the donor testis tissue form 2-week-, 5-month- and 8-month-old colts contained only immature Sertoli cells and gonocytes. No spermatogenic differentiation occurred in xenografts from the 2-week-old colt and testosterone production was minimal. Pachytene spermatocytes were observed in testis grafts from the 5- and 8-month-old donors from 14 weeks onward. Spermatogenesis did not proceed through meiosis in grafts from the 5-month-old donor. Recipient mice carrying xenografts from the 8-month-old donor received exogenous gonadotropins (equine chorionic gonadotropin and human chorionic gonadotropin, 10 I.U./day for 2 months, beginning 14 weeks after grafting) and condensing spermatids were observed by 35 weeks after grafting. In donor tissue from the 10-month-old colt, pachytene spermatocytes were present in about 50% of tubules at the time of grafting. After 14 weeks, xenografts showed fewer differentiated germ cells than the donor tissue. However, at 35 weeks after grafting, condensing spermatids were observed, indicating that differentiated germ cells were initially lost and spermatogenesis was subsequently reinitiated. In all castrated host mice where spermatogenic differentiation occurred, the weight of the seminal vesicles was restored to pre-castration values showing that xenografts were releasing bioactive testosterone. These results indicate that horse spermatogenesis can occur in a mouse host albeit with low efficiency. Testicular maturation was not accelerated. In most cases, spermatogenesis appeared to become arrested at meiosis. The underlying mechanisms of this spermatogenic arrest require further investigation. Although equine testis xenografts produced testosterone, supplementation of exogenous gonadotropins might support post-meiotic differentiation. This work was supported by USDA 03-35203-13486.

In Situ Monitoring of Chondrocyte Response to Bioactive Scaffolds Using Raman Spectroscopy

2005 ◽  
Vol 284-286 ◽  
pp. 623-626 ◽  
Author(s):  
Julian R. Jones ◽  
Archana Vats ◽  
Ioan Notingher ◽  
Julie E. Gough ◽  
Neil S. Tolley ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Septal Cartilage ◽  
In Situ Monitoring ◽  
Invasive Technique ◽  
3D Scaffold ◽  
Bioactive Scaffolds ◽  
Non Invasive ◽  
Glass Foam

Septal cartilage is widely used for the repair of soft tissue defects in the head, neck and nose. Tissue Engineering techniques are being investigated to create cartilage in vitro by seeding appropriate cells on resorbable scaffolds. In this study, human chondrocytes were cultured on macroporous bioactive glass foam scaffolds. The aim was to investigate how Raman spectroscopy could be used as a non-invasive technique to monitor the response of chondrocytes to a 3D scaffold in real time. The spectra were compared to scanning electron microscope (SEM) micrographs and immunohistochemistry results.

scholarly journals Non-invasive in situ monitoring of bone scaffold activity by speckle pattern analysis

2020 ◽  
Vol 11 (11) ◽  
pp. 6324
Author(s):  
Vahideh Farzam Rad ◽  
Majid Panahi ◽  
Ramin Jamali ◽  
Ahmad Darudi ◽  
Ali-Reza Moradi
Keyword(s):  
Pattern Analysis ◽  
Speckle Pattern ◽  
In Situ Monitoring ◽  
Bone Scaffold ◽  
Non Invasive
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