scholarly journals Optimization of Extraction Conditions for Antioxidant Activity of Acer tegmentosum Using Response Surface Methodology

2021 ◽  
Vol 11 (3) ◽  
pp. 1134
Author(s):  
Inyong Kim ◽  
Jung-Heun Ha ◽  
Yoonhwa Jeong
Keyword(s):  
Hydrogen Peroxide ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Hepg2 Cells ◽  
Extraction Methods ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Freeze Dried ◽  
Hydrothermal Extraction

Extraction from edible plants is a highly important process that has various biological functions. To maximize biological activity, extraction methods should facilitate optimal extraction of functional phytochemicals. In this study, the optimal hydrothermal extraction conditions of Acer tegmentosum were determined using response surface methodology (RSM), and HepG2 cells were treated with optimized extract and hydrogen peroxide. In a central composition design, the independent variables were extraction temperature (X1: 70–90 °C), extraction time (X2: 2–6 h), and solvent-to-solid ratio (X3: 50–150). The maximum total phenolic contents (276.70 ± 10.11 mg GAE/g) and DPPH (2,2-diphenyl-1-pictylhydrazyl) activity (33.45 ± 2.20%) of A. tegmentosum were estimated at optimized extraction conditions, as follows: X1: 89.34 °C, X2: 7.36 h, X3: 184.09. Using the calculated extraction conditions, functional phytochemicals were extracted by hydrothermal extraction and freeze-dried. A. tegmentosum treatment (>10 μg/mL) of HepG2 cells remarkably attenuated hydrogen-peroxide-inducible hepatic cellular death and reactive oxygen species production in vitro.

scholarly journals Development of Phaleria macrocarpa (Scheff.) Boerl Fruits Using Response Surface Methodology On Phenolic, Flavonoid and Antioxidants Properties

2018 ◽  
Author(s):  
Khurul Ain Mohamed Mahzir ◽  
Siti Salwa Abdul Gani ◽  
Nor Fadzillah Mokhtar
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Extraction Time ◽  
Feed Ratio ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Phaleria Macrocarpa

In this study, the optimal condition for the extraction of antioxidants from the fruit Buah Mahkota Dewa (Phaleria macrocarpa) was determined by using Response Surface Methodology (RSM). The optimization was applied using central composite design (CCD) to investigate the three independent variables, namely extraction temperature (oC), extraction time (minutes) and extraction solvent to-feed ratio (%v/v) on the responses of free radical scavenging activity (DPPH), ferric ion reducing power assay (FRAP), total phenolic content (TPC) and total flavonoid content (TFC).The optimal conditions for the antioxidants extraction were found to be extraction temperature (64oC), extraction time (66 minutes) and solvent to-feed ratio (75 %v/v) with the highest percentage yield of DPPH, FRAP, TPC and TFC were 86.85%, 7.47%, 292.86 mg/g and 3.22 mg/g respectively. Moreover, the data were subjected to response surface methodology (RSM) and the results showed that the polynomial equations for all models were significant, did not show lack of fit, and presented adjusted determination coefficients (R2) above 99%, proving the yield of phenolic, flavonoid and antioxidants activities obtained experimentally were close to the predicted values and the suitability of the model employed in RSM to optimize the extraction conditions. Hence, in this study, the fruit from P.macrocarpa could be considered to have the strong antioxidant ability and can be used in various cosmeceutical or medicinal applications.

scholarly journals Effects of Time, Temperature, and Solvent Ratio on the Extraction of Non-Extractable Polyphenols with Anticancer Activity of Barhi Date Palm Kernels Extracts Using Response Surface Methodology

2019 ◽  
Author(s):  
Israa Mahmud ◽  
Mohamed E. S. Mirghani ◽  
Faridah Yusof ◽  
Ma'an Al-khatib
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Date Palm ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Solvent Ratio ◽  
Extraction Temperature ◽  
Dietary Polyphenols ◽  
Experimental Values

Dietary polyphenols exist in two forms; extractable polyphenols (EPP) or compounds solubilised by aqueous/organic solvents, and non-extractable polyphenols (NEPP) or compounds remain in the corresponding residues after the extraction. At present, most researchers focus on EEP fractions, while NEPP is neglected. Thus, this study aimed to release NEPP from the remaining powder residue of Barhi date palm kernels (BDPK) with acid hydrolysis. The related extraction conditions were determined and optimised using response surface methodology (RSM) for maximisation of NEPP with highest cytotoxic and antioxidant activities. The face-centred central composite design (FCCCD) was used to establish treatments based on three independent variables, namely; extraction temperature, time, and solvent/sample ratio. Under the optimal conditions, the experimental values for DPPH radical-scavenging capacity of NEPP (IC50=57.52µg/mL), and cytotoxicity of NEPP against A549 and HT29 cells were IC50=17.4 µg/mL and 31.4µg/mL, respectively. The experimental values were in agreement with those predicted by RSM models, confirming the suitability of the model employed and the success of RSM for optimisation of the extraction conditions for NEPP from BDPK. These results indicate that NEPP from industrial date fruit waste could be a promising candidate as natural antioxidants with significant antiproliferation effect against A549 and HT29 cancer cells in-vitro.

scholarly journals Optimization of phenolic compounds extraction with antioxidant activity from açaí, blueberry and goji berry using response surface methodology

2018 ◽  
pp. 180 ◽  
Author(s):  
Cristiane de Moura, Amália Soares dos Reis ◽  
Letícia Dangui da Silva, Vanderlei Aparecido de Lima ◽  
Tatiane Luiza Cadorin Oldoni ◽  
Celeide Pereira ◽  
Solange Teresinha Carpes
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Phenolic Compounds ◽  
Response Surface ◽  
High Performance ◽  
Total Phenolic ◽  
Global Response ◽  
Goji Berry ◽  
Berry Fruits

The aim of this study was to determine the best extraction conditions of phenolic compounds present in açaí, blueberry and goji berry fruits using the response surface methodology (RSM). The phenolic compounds profile by high-performance liquid chromatography, antioxidant activity was also determined. A factorial 23 design was used to analyze the effect of the solvent (ethanol and water), time (30 and 60 min) and temperature (30 °C and 60 °C) on the extraction of total phenolic compounds (TPC) and activity antioxidant (AA). The variables time and temperature had a positive effect on antioxidant activity (AA) in their highest levels, 60 min and 60 °C, respectively. The solvent ethanol 800 g/mL was more efficient in TPC extracting with AA in all matrices. Rutin was present in high amounts in blueberry and goji berry, and the myricetin in açaí. The açaí showed higher in vitro antioxidant activity when extracted at 60 °C for 60 min. The high correlation coefficient (0.98) of global response (GR) showed that we can find out single and global response in research with multiple dependent variables.  The GR analysis indicated the highest values of the TPC and AA when the fruits were extracted at 60°C for 60 min using ethanol as solvent and it was very useful for simplifying and improving the phenolic compounds extraction performance.

scholarly journals Optimasi Ekstraksi Berbantu Gelombang Ultrasonik pada Biji Melinjo Kerikil (Gnetum gnemon L., ‘Kerikil’) Menggunakan Response Surface Methodology

2019 ◽  
Vol 8 (3) ◽  
pp. 104
Author(s):  
Bambang Kunarto ◽  
Sutardi Sutardi ◽  
Supriyanto Supriyanto ◽  
Chairil Anwar
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Reducing Power ◽  
Extraction Process ◽  
Extraction Methods ◽  
Total Phenolic ◽  
Ultrasonic Bath ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction

Biji melinjo kerikil mengandung senyawa fenolik dan resveratrol sehingga berpotensi sebagai antioksidan, oleh karena itu perlu dilakukan ekstraksi. Metoda ekstraksi konvensional (maserasi, perebusan, refluxing) mempunyai kelemahan yaitu terjadinya kerusakan senyawa fenolik akibat reaksi oksidasi, hidrolisis dan ionisasi selama proses ekstraksi. Oleh karena itu, pada penelitian ini ekstraksi biji melinjo kerikil dilakukan menggunakan metode ekstraksi berbantu gelombang ultrasonik. Penelitian ini bertujuan untuk optimasi ekstraksi biji melinjo kerikil berbantu gelombang ultrasonik yang dilakukan pada berbagai waktu ekstraksi, suhu ekstraksi dan konsentrasi pelarut etanol. Surface response methodology digunakan untuk optimasi kondisi ekstraksi. Biji melinjo kerikil diekstrak menggunakan ultrasonic bath pada frekuensi 40 kHz dengan berbagai suhu (25, 30, 35, 40, 45oC), waktu ekstraksi (10, 20, 30, 40, 50 menit) dan konsentrasi pelarut etanol (40, 50, 60, 70 80%). Hasil penelitian menunjukkan bahwa kondisi optimum ekstraksi biji melinjo kerikil berbantu gelombang ultrasonik adalah pada suhu 30,18oC, waktu 33,01 menit dan konsentrasi pelarut etanol 71,04%. Pada kondisi ini, diperoleh yield ekstrak 18,41 ± 0,01%, total fenolik 11,26 ± 0,06 mg GAE/g, total flavonoid 533,70 ± 0,18 mg CE/100g, resveratrol 7,64 ± 0, 03%, IC50 sebesar 59,52 ± 0,04 ppm dan reducing power 76,31 ± 0,08%. Sebagai kesimpulan, optimasi ekstraksi biji melinjo kerikil berbantu gelombang ultrasonik menggunakan response surface methodology ini cukup baik karena nilai respon yang sebenarnya sesuai dengan nilai respon yang diprediksi.Melinjo kerikil seeds contain phenolic and resveratrol compounds so that it has the potential as an antioxidant, therefore extraction needs to be done. Conventional extraction methods (maceration, boiling, refluxing) have the disadvantage of devasting phenolic compounds due to oxidation, hydrolysis and ionization reactions during the extraction process. Therefore, in this study the extraction of melinjo kerikil seeds done by using the ultrasonic-assisted extraction method. The study aims to optimize ultrasonic-assisted extraction of melinjo kerikil seeds that be done at various extraction times, extraction temperatures and ethanol solvents concentrations. Response surface methodology was used to optimize experimental condition for extraction. Melinjo kerikil seeds were extracted by using ultrasonic bath at a frequency of 40 kHz with various temperatures (25, 30, 35, 40, 45oC), extraction time (10, 20, 30, 40, 50 minutes) and ethanol solvents concentrations (40, 50, 60, 70 80 %). The results showed that the optimum conditions for ultrasonic-assisted extraction of melinjo kerikil seeds were at a temperature of 30.18oC, a time of 33.01 minutes and an ethanol solvent concentration of 71.04%. In this condition, obtained the extract yield 18.41 ± 0.01%, total phenolic 11.26 ± 0.06 mg GAE/g, total flavonoids 533.70 ± 0.18 mg CE/100g, resveratrol 7.64 ± 0,03%, IC50 in the amount of 59.52 ± 0.04 ppm and reducing power 76.31 ± 0.08%. As a conclusion, the optimization ultrasonic-assisted extraction of melinjo kerikil seeds by using response surface methodology is quite good because the actual response value is in accordance with the predicted response value.

Optimization of Antioxidant Extraction from Freeze-dried Pulp, Peel, and Seed of Burmese grape (Baccaurea ramiflora Lour.) by Response Surface Methodology

2021 ◽  
Author(s):  
Mohammad Afzal Hossain ◽  
Md. Sakib Hossain
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Incubation Time ◽  
Maximum Yield ◽  
Extraction Procedure ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Solvent Concentration ◽  
Antioxidant Power ◽  
Freeze Dried

Abstract This study aimed to attain the optimum condition necessary for extracting the maximum yield of antioxidants from the freeze-dried pulp, peel, and seed of Burmese grape using response surface methodology (RSM). Solvent (ethanol) concentration (%), temperature (°C), and time (min) were taken as independent variables by factorial screening for the extraction procedure. After extraction, the antioxidant activity of all samples was determined employing 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, total phenolic compounds (TPC), and ferric reducing antioxidant power (FRAP) assay. The experiment's optimum conditions were 80% solvent concentration, 69.01°C temperature, and 30 min for pulp. The optimum extraction conditions were found at 80°C for 29.39 min incubation time using 52.12% concentrated solvent for seed. For peel, the solvent concentration of 41.62% was found optimum when the temperature of 50°C and 30 min incubation time were used. The actual values of TPC, FRAP, and DPPH for freeze-dried pulp, peel and seed extracts were close to the predicted values, which confirms the models’ validity. The Analysis of Variance (ANOVA) showed that the models were significant for TPC, DPPH, and FRAP values of peel, pulp, and seed at different levels (p < 0.001 to p < 0.05). The composite desirability of pulp, seed, and peel were 0.94, 0.98, and 0.85, respectively, which suggest that the developed model could be effectively used for antioxidants’ extraction from freeze-dried pulp, peel, and seed of Burmese grape.

scholarly journals Optimization of Extraction of Total Phenolic and Total Flavonoid from Seeds of Alpinia blepharocalyx K. Schum. Using The Response Surface Methodology

2019 ◽  
Vol 57 (3B) ◽  
pp. 105
Author(s):  
Thanh Tan Nguyen ◽  
Huyen Thi Thanh Mai ◽  
Tuan Ngoc Nguyen ◽  
Quang Dinh Tran ◽  
Thang Dinh Tran
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Antioxidant Properties ◽  
Total Flavonoid ◽  
Extraction Time ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Flavonoid Compounds ◽  
Phenolic And Flavonoid ◽  
Phenolic And Flavonoid Compounds

Seeds of Alpinia blepharocalyx contain high amounts of phenolic and flavonoid compounds with potential antioxidant properties. In this study, the effects of the extraction method on the recovery of phenolic and flavonoid compounds from Alpinia blepharocalyx seeds were investigated. Response surface methodology (RSM) has been used to optimize the extraction conditions of total phenolic and total flavonoid from seeds of Alpinia blepharocalyx. A Box-Behnken design was used to investigate the effects of four independent variables, namely extraction temperature (°C), extraction time (min), (v/m) and ethanol concentration (%) on the responses: total phenolic content (TPC) and yield. The optimal conditions obtained from response RSM were 52.66 % v/v for the solvent composition, 62.34 (°C) for extraction temperature, 34.48/1 (ml/g) for solvent/material ratio and 125.42 (min) for extraction time. The experimental values of TPC, TFC and yield were 39.31±0.05 mgGAE/g, 12.75±0.07 mgCE/g and 6.97±0.05%, respectively.   

Optimization of Aqueous Extraction of the Australian Maroon Bush (Scaevola spinescens R. Br.) to Maximize Bioactive Compound and Antioxidant Yield

2021 ◽  
Vol 02 ◽  
Author(s):  
Kien Q. Nguyen ◽  
Christopher J. Scarlett ◽  
Quan V. Vuong
Keyword(s):  
Response Surface Methodology ◽  
Antioxidant Capacity ◽  
Response Surface ◽  
Bioactive Compounds ◽  
Aqueous Extraction ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Isolation And Purification ◽  
Temperature Time ◽  
Water Ratio

Background: Scaevola spinescens is an endemic Australian shrub that is linked to various health benefits and traditionally used as medicine by decoction. To date, the extraction efficiency of the plant under various conditions has not been well understood. This study aimed to optimize aqueous extraction conditions of S. spinescens, for maximum extraction of total phenolic compounds, flavonoids and saponins, as well as antioxidant activities. Methods: Response surface methodology was used to determine the influence of four independent parameters including temperature, time, sample-to-water ratio and pH. The optimal ranges of temperature (60-90 °C), time (30-60 min), sample-to-water ratio (2-6 g/100 mL) and pH (3-7) were determined in preliminary experiments. Following assessment and optimization of the response surface methodology models, validation experiments were conducted to compare predicted and experimental values. Results : The RSM models showed that extraction temperature, time and sample-to-water ratio significantly affected total phenolic compound yields. Extraction temperature and time significantly affected flavonoid yields, while only sample-to-water ratio significantly affected saponin yields. Optimal conditions for extraction were determined to be: 90 ºC, 53 min, 2:100 (g/mL), and pH of 4.5, if saponins are the target compounds for extraction. For phenolics, flavonoids and antioxidant capacity, a higher sample-to-water ratio of 6:100 (g/mL) is recommended. Conclusion: Response surface methodology proved to be a reliable method for predicting yields of bioactive compounds and antioxidant capacity in S. spinescens. These findings can be used for efficient decoction by practitioners and end users, or by researchers for further isolation and purification of bioactive compounds from S. spinescens extracts.

scholarly journals Extraction Optimization of Phenolics and Antioxidants from Black Goji Berry by Accelerated Solvent Extractor Using Response Surface Methodology

2018 ◽  
Vol 8 (10) ◽  
pp. 1905 ◽  
Author(s):  
Qinghua He ◽  
Bin Du ◽  
Baojun Xu
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Maximum Yield ◽  
Scientific Basis ◽  
Total Phenolic ◽  
Optimum Process ◽  
Extraction Temperature ◽  
Box Behnken Design ◽  
Goji Berry ◽  
Phenolic Substances

This study aims to provide fundamental knowledge for the use of black goji berry and determine the optimum process parameters that produce maximum phenolics from black goji berry through accelerated solvent extraction (ASE). The optimal extractions of phenolics and antioxidants from black goji were explored via the ASE techniques, applying the response surface methodology (RSM) design. After reaching the optimal conditions for single factors, the optimal ASE extraction conditions were found through a total of 17 runs following the Box-Behnken design (BBD) from RSM. The maximum yield of total phenolic content (TPC) was 17.92 mg GAE/g under the best extraction conditions: an extraction temperature of 89.38 °C, an ethanol concentration of 70% and an extraction time of 13 min. This study indicates that the optimal extraction conditions could serve as the scientific basis for scaled-up industrial production. Black goji berry could be a viable source of nutraceuticals due to the abundance of antioxidant and phenolic substances.

scholarly journals Flavonoids from Morus alba L. Leaves: Optimization of Extraction by Response Surface Methodology and Comprehensive Evaluation of Their Antioxidant, Antimicrobial, and Inhibition of α-Amylase Activities through Analytical Hierarchy Process

Molecules ◽  
2019 ◽  
Vol 24 (13) ◽  
pp. 2398 ◽  
Author(s):  
Hao Cui ◽  
Tenghui Lu ◽  
Mingxin Wang ◽  
Xintong Zou ◽  
Yang Zhang ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Analytical Hierarchy Process ◽  
Comprehensive Evaluation ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Morus Alba ◽  
Extraction Temperature ◽  
Hierarchy Process

To explore the flavonoids from Morus alba L. leaves (MLF), the process of extracting was optimized by a response surface methodology and the antimicrobial and antioxidant activities were evaluated in vitro. The yield of flavonoids reached 50.52 mg g−1 under the optimized extraction conditions (i.e., extraction temperature, 70.85 °C; solvent concentration, 39.30%; extraction time, 120.18 min; and liquid/solid ratio, 34.60:1). The total flavonoids were extracted in organic solvents with various polarities, including petroleum ether (MLFp), ethyl acetate (MLFe), and n-butanol (MLFb). In vitro, the four MLF samples exhibited good antioxidant activities for scavenging of 2, 2′-azinobis-(3-ethylbenz-thiazoline-6-sulphonate) radical, 1, 1-diphenyl-2-picrylhydrazyl radical, and total reducing power. Regarding antimicrobial efficacy, the MLF samples suppressed the development of Staphylococcus aureus, Bacillus subtilis, and Bacillus pumilus. The MLF samples inhibited α-amylase activity to a certain extent. The analytical hierarchy process (AHP) was used to evaluate comprehensively the bioactivities of the MLF samples. The AHP results revealed that the bioactivity comprehensive score (78.83 μg mL−1) of MLFe was optimal among the four MLF samples. Morus alba L. leaves also exhibited non-hemolytic properties. All bioactivities suggested the potential of MLFe as a candidate resource in the food and drug industries.

scholarly journals Optimization of Reflux Conditions for Total Flavonoid and Total Phenolic Extraction and Enhanced Antioxidant Capacity in Pandan (Pandanus amaryllifoliusRoxb.) Using Response Surface Methodology

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Ali Ghasemzadeh ◽  
Hawa Z. E. Jaafar
Keyword(s):  
Response Surface Methodology ◽  
Antioxidant Capacity ◽  
Response Surface ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Solid Ratio ◽  
Central Composite Experimental Design ◽  
Operation Parameters ◽  
Predicted Values ◽  
Experimental Values

Response surface methodology was applied to optimization of the conditions for reflux extraction of Pandan (Pandanus amaryllifoliusRoxb.) in order to achieve a high content of total flavonoids (TF), total phenolics (TP), and high antioxidant capacity (AC) in the extracts. Central composite experimental design with three factors and three levels was employed to consider the effects of the operation parameters, including the methanol concentration (MC, 40%–80%), extraction temperature (ET, 40–70°C), and liquid-to-solid ratio (LS ratio, 20–40 mL/g) on the properties of the extracts. Response surface plots showed that increasing these operation parameters induced the responses significantly. The TF content and AC could be maximized when the extraction conditions (MC, ET, and LS ratio) were 78.8%, 69.5°C, and 32.4 mL/g, respectively, whereas the TP content was optimal when these variables were 75.1%, 70°C, and 31.8 mL/g, respectively. Under these optimum conditions, the experimental TF and TP content and AC were 1.78, 6.601 mg/g DW, and 87.38%, respectively. The optimized model was validated by a comparison of the predicted and experimental values. The experimental values were found to be in agreement with the predicted values, indicating the suitability of the model for optimizing the conditions for the reflux extraction of Pandan.

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