scholarly journals Laser-Induced Forward Transfer with Optical Stamp of a Protein-Immobilized Calcium Phosphate Film Prepared by Biomimetic Process to a Human Dentin

2020 ◽  
Vol 10 (22) ◽  
pp. 7984
Author(s):  
Aiko Narazaki ◽  
Ayako Oyane ◽  
Hirofumi Miyaji
Keyword(s):  
Calcium Phosphate ◽  
Surface Functionalization ◽  
Pdms Substrate ◽  
Beam Spot ◽  
Cross Sectional ◽  
Adhesion Protein ◽  
Forward Transfer ◽  
Human Dentin ◽  
Hard Substrates ◽  
Phosphate Film

The rapid and area-specific printing of calcium phosphate with superior biocompatibility and osteoconductivity is a useful technique for the surface functionalization of biomedical devices. We recently demonstrated the laser-induced forward transfer (LIFT) of a brittle calcium phosphate film onto a soft and shock-absorbing polydimethylsiloxane (PDMS) substrate. In this work, a new LIFT using an optically transparent PDMS-coated stamp, which we hereafter call LIFT with optical stamp (LIFTOP), was introduced to achieve the transfer of brittle films to harder substrates. Cell adhesion protein fibronectin-immobilized calcium phosphate films (Fn-CaP) were prepared on the optical stamp through a biomimetic process. Then, the irradiation of a single laser pulse transferred the Fn-CaP film from the optical stamp onto relatively hard substrates, polyethylene terephthalate and human dentin. As a result of this LIFTOP process, Fn-CaP microchips with a shape corresponding to the laser beam spot were printed on the substrates. Cross-sectional observation of the interface between the Fn-CaP microchip and the dentin substrate revealed good attachment between them without obvious gaps for the most part.

Effect of Substrate Temperature on Properties of Nano-Scale Functionally Graded Calcium Phosphate Coatings

2006 ◽  
Author(s):  
Travis Blalock ◽  
Xiao Bai ◽  
Afsaneh Rabiei
Keyword(s):  
Electron Microscopy ◽  
Calcium Phosphate ◽  
Substrate Temperature ◽  
Ion Beam ◽  
Processing Parameters ◽  
Functionally Graded ◽  
Cross Sectional ◽  
X Ray ◽  
Phosphate Coatings ◽  
Calcium Phosphate Coatings

The effect of substrate temperature and processing parameters on microstructure and crystallinity of calcium phosphate coatings deposited on heated substrates in an Ion Beam Assisted Deposition (IBAD) system are being studied. The experimental procedures include mechanical testing and film thickness measurements using bonding strength and profilometery. Cross-sectional scanning transmission electron microscopy (STEM) with energy dispersive X-ray spectroscopy (EDX) through the thickness of the film as well as scanning electron microscopy (SEM) with EDX at the top surface of the film was performed to evaluate the microstructure of the film. The coating crystallinity was studied through X-ray diffraction (XRD). The information gained from current analysis on the set temperature coatings will be used to refine the processing techniques of the Functionally Graded Hydroxyapatite (FGHA) coating.

scholarly journals Laser-assisted wet coating of calcium phosphate for surface-functionalization of PEEK

PLoS ONE ◽  
2018 ◽  
Vol 13 (10) ◽  
pp. e0206524 ◽  
Author(s):  
Ayako Oyane ◽  
Maki Nakamura ◽  
Ikuko Sakamaki ◽  
Yoshiki Shimizu ◽  
Saori Miyata ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Surface Functionalization

Surface functionalization of cuttlefish bone-derived biphasic calcium phosphate scaffolds with polymeric coatings

2019 ◽  
Vol 105 ◽  
pp. 110014 ◽  
Author(s):  
Ana S. Neto ◽  
Ana C. Fonseca ◽  
J.C.C. Abrantes ◽  
Jorge F.J. Coelho ◽  
José M.F. Ferreira
Keyword(s):  
Calcium Phosphate ◽  
Surface Functionalization ◽  
Biphasic Calcium Phosphate ◽  
Polymeric Coatings

scholarly journals In Vitro Study on the Effect of a New Bioactive Desensitizer on Dentin Tubule Sealing and Bonding

2020 ◽  
Vol 11 (2) ◽  
pp. 38
Author(s):  
Minh N. Luong ◽  
Laurie Huang ◽  
Daniel C. N. Chan ◽  
Alireza Sadr
Keyword(s):  
Bond Strength ◽  
In Vitro Study ◽  
Vitro Study ◽  
Acid Pretreatment ◽  
Universal Adhesive ◽  
Cross Sectional ◽  
Before And After ◽  
Human Dentin ◽  
Microshear Bond Strength

Bioactive mineral-based dentin desensitizers that can quickly and effectively seal dentinal tubules and promote dentin mineralization are desired. This in vitro study evaluated a novel nanohydroxyapatite-based desensitizer, Predicta (PBD, Parkell), and its effect on bond strength of dental adhesives. Human dentin discs (2-mm thick) were subjected to 0.5 M EDTA to remove the smear layer and expose tubules, treated with PBD, and processed for surface and cross-sectional SEM examination before and after immersion in simulated body fluid (SBF) for four weeks (ISO 23317-2014). The effects of two dental desensitizers on the microshear bond strength of a universal adhesive and a two-step self-etch system were compared. SEM showed coverage and penetration of nanoparticles in wide tubules on the PBD-treated dentin at the baseline. After four weeks in SBF, untreated dentin showed amorphous mineral deposits while PBD-treated dentin disclosed a highly mineralized structure integrated with dentin. Desensitizers significantly reduced microshear bond strength test (MSBS) of adhesives by 15–20% on average, depending on the bonding protocol. In conclusion, PBD demonstrated effective immediate tubules sealing capability and promoted mineral crystal growth over dentin and into the tubules during SBF-storage. For bonding to desensitizer-treated dentin, a two-step self-etching adhesive or universal bond with phosphoric acid pretreatment are recommended.

Calcium Phosphate Coating on Titanium Plates by Electrodeposition

2001 ◽  
Vol 711 ◽  
Author(s):  
Masahiko Ishikawa ◽  
Kouji Nishikawa ◽  
Kensuke Kuroda ◽  
Ichino Ryoichi ◽  
Masazumi Okido ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Electrochemical Process ◽  
Calcium Phosphate Coating ◽  
X Ray Diffraction ◽  
Cathodic Current ◽  
Solution Ph ◽  
Cathodic Potential ◽  
Peeling Test ◽  
Film Adhesion ◽  
Phosphate Film

ABSTRACTCalcium phosphate films were deposited on titanium electrodes cathodically from CaCl2·2H2O and Ca(H2PO4)2 · H2O aqueous solutions. In this study, H2O2 addition into electrolytes was applied to enhance the electrochemical process at the solution/electrode at a smaller cathodic potential than no H2O2 addition. Deposited films were analyzed by scanning electron microscopy (SEM) observation and X-ray diffraction (XRD). Cathodic current of the Ti electrode decreased once and increased in the solution with H2O2. It shows a cathodic current peak at c.a. 25 min in the case of potentiostatic condition at - 0.756 V (vs. Ag - AgCl, sat. KCl). The calcium phosphate film grows mainly with the decrease in current after the cathodic peak. The characteristics for the electrodeposited film such as crystal morphology depends on cathodic potential, solution pH, deposition temperature and amount of H2O2 addition. Dense calcium phosphate film composed of relatively good crystalline was obtained at pH 5.5 and – 0.756 V. Film adhesion on Ti appeared to be strong by peeling test. At larger cathodic potential of – 1.156 V, the film coverage on titanium plates was smaller and film adhesion worsened. Larger cathodic polarization of more than – 1.556 V was necessary to reduce water in case without H2O2 addition.

Arteriosclerosis in Dialysis Patients

1996 ◽  
Vol 19 (11) ◽  
pp. 638-644 ◽  
Author(s):  
J. Bommer ◽  
E. Strohbeck ◽  
J. Goerich ◽  
M. Bahner ◽  
I. Zuna
Keyword(s):  
Calcium Phosphate ◽  
Hdl Cholesterol ◽  
Cross Sectional Study ◽  
Hemodialysis Patients ◽  
Chronic Hemodialysis ◽  
Maintenance Hemodialysis ◽  
Time Interval ◽  
Dialysis Patients ◽  
Cross Sectional ◽  
Vldl Cholesterol

Arteriosclerosis is a constant problem in long-term hemodialysis patients. Computer tomography of the abdominal aorta allows a well-defined and reproducible evaluation of aortosclerosis. In the cross-sectional study, aortosclerosis was significantly accelerated in 84 chronic hemodialysis patients and was comparable to the results found in 20-year older control patients without renal disease. The increase of aortosclerosis correlated significantly with age of the patient, smoking, and duration of dialysis therapy. Furthermore, increased VLDL cholesterol and decreased HDL cholesterol seem to enhance aortosclerosis in our dialysis patients. In the longitudinal study (two CT scans with a time interval of 87 ± 62.7 months) in 36 dialysis patients, progressed aortosclerosis correlated significantly with the long duration of hypertriglyceridemia, VLDL cholesterol, uric acid, and calcium phosphate products. Progression of aortosclerosis was reduced in parathyroidectomized patients. The study suggests that premature aortosclerosis is found in dialysis patients. In addition to the common risk factor of aortosclerosis, disturbed calcium phosphate and parathyroid hormone metabolism seem to enhance aortosclerosis in patients under maintenance hemodialysis.

Polyester fibers can be rendered calcium phosphate-binding by surface functionalization with bisphosphonate groups

2017 ◽  
Vol 105 (8) ◽  
pp. 2335-2342 ◽  
Author(s):  
Alessandro Polini ◽  
Daniela Geta Petre ◽  
Michele Iafisco ◽  
Sonia de Lacerda Schickert ◽  
Anna Tampieri ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Surface Functionalization ◽  
Phosphate Binding ◽  
Polyester Fibers
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