scholarly journals Impact of Phosphorus on Cannabis sativa Reproduction, Cannabinoids, and Terpenes

2020 ◽  
Vol 10 (21) ◽  
pp. 7875
Author(s):  
Paul Cockson ◽  
Michelle Schroeder-Moreno ◽  
Patrick Veazie ◽  
Gabby Barajas ◽  
David Logan ◽  
...  

Many abiotic factors, such as mineral nutrients—including phosphorus (P)—fertility, can impact the yield and growth of Cannabis sativa. Given the economic portion of C. sativa is the inflorescence, the restriction of P fertility could impact floral development and quality could be detrimental. This study sought to track the impacts of varying P concentrations (3.75, 7.50, 11.25, 15.0, 22.50, and 30.0 mg·L−1) utilizing a modified Hoagland’s solution. This experiment examined plant height, diameter, leaf tissue mineral nutrient concentrations, and final fresh flower bud weight as well as floral quality metrics, such as cannabinoids and terpenes. The results demonstrated that during different life stages (vegetative, pre-flowering, flowering), P concentrations impact C. sativa growth and development and yield. Regarding the cannabinoid pools, results varied for the individual cannabinoid types. For the acid pools, increasing fertility concentrations above 11.25 mg·L−1 P did not result in any increase in cannabinoid concentrations. These results indicate that, if a crop is being produced under greenhouse conditions, specifically for cannabinoid production, an excessive P supply did not result in higher cannabinoid production. However, plants grown with a higher rate of P fertility (30.0 mg·L−1) had greater plant width and may result in more buds per plant.

Agriculture ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 221
Author(s):  
Paul Cockson ◽  
Patrick Veazie ◽  
Matthew Davis ◽  
Gabby Barajas ◽  
Angela Post ◽  
...  

Many abiotic factors impact the yield and growth of Brassica carinata (commonly referred to as carinata or Ethiopian mustard). Very little is known about carinata and how mineral nutrients impact its growth, and more specifically, the sufficiency values for fertility over the plant’s growth cycle and life stages. This study explored the impacts that plant nutrients, specifically micronutrients, can have on the growth and development of carinata over its distinct life stages (rosette, bolting, flowering, and pod set). Plants were grown under varying micronutrient concentrations (0, 25, 50, 75, 87.5, and 100%) of a modified Hoagland’s solution. Data were collected on plant height, canopy diameter, leaf tissue mineral nutrient concentrations, and biomass. The results demonstrated that micronutrient fertility has profound impacts on the production of Brassica carinata during different life stages. Boron (B) exclusion had the greatest impact on the growth and reproduction of Brassica carinata, with the death of the apical meristem that resulted in a lack of siliques or seeds at the lowest rate. Optimal relative elemental leaf tissue concentrations varied among micronutrient fertility concentrations and life stages. Certain elements exhibited linear increases in nutrient leaf tissue accumulation as solution concentration increased without reaching a maximum concentration during specific life stages. Other life stages and/or elements produced distinct plateau leaf tissue mineral concentrations despite increasing fertility treatment concentrations such as B in the rosette stage (47.2–50.0 mg·kg−1), copper (Cu) (bolting stage at 6.62–7.57 mg·kg−1), zinc (Zn) (bolting stage at 27.47–39.87 and flowering at 33.98–43.50 mg·kg−1), molybdenum (Mo) (flowering stage at 2.42–3.23 mg·kg−1), and manganese (Mn) (bolting stage at 117.03–161.63 mg·kg−1). This work demonstrates that Brassica carinata has different fertility demands and will accumulate differing leaf tissue concentrations during its life stages. This work serves as a baseline for further uptake and portioning work for Brassica carinata.


HortScience ◽  
2016 ◽  
Vol 51 (5) ◽  
pp. 504-509 ◽  
Author(s):  
T. Casey Barickman ◽  
Thomas E. Horgan ◽  
Jennifer R. Wheeler ◽  
Carl E. Sams

Lettuce is one of the major crops of the United States and can provide a large portion of income for small to medium size growers. Growing lettuce in adverse environmental conditions can have negative effects on quality. Elevated levels of potassium (K) have been shown to positively influence quality in various fruits and vegetables, such as tomato, pepper, and strawberry. However, research is lacking on the effects of elevated levels of K on leafy vegetables such as lettuce. Therefore, seeds of ‘Cimmaron’ lettuce were sown into a soilless medium and grown in greenhouse conditions at 25/20 °C (day/night). At 27 days after seeding, the plantlets were transferred to 3.8-L plastic nursery pots. Plants were grown under increasing K treatments of 98 (control), 185 (2×), 370 (3×), and 740 (8×) kg·ha−1. Plants were harvested 56 days after seeding. Application of elevated levels of K fertilizer treatments in red romaine lettuce had a positive quadratic effect on plant height increasing 7.0% from the control. Fresh weight (FW) increased 13.0% from the control and dry weight (DW) increased 15.5%. There was linear increase of 30.0% in sucrose concentrations in lettuce leaf tissue. In addition, the increase in K treatments caused an increase of 43.3% in K concentrations in the leaf tissue. In other nutrients, such as Calcium (Ca), Magnesium (Mg), and Sulfur (S), there was a decrease in the leaf tissue of 61%, 52%, and 46% when compared with the control treatment, respectively. The results of the current study suggest that increasing K fertilizer to 185 kg·ha−1 has the best results for plant height, FW and DW, and mineral nutrient concentrations. This study may initiate research that could examine the effects of increasing K fertilizer levels in lettuce or other leafy green vegetables on antioxidant levels and postharvest storability.


HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 529d-529
Author(s):  
J.M. Smagula ◽  
W. Litten ◽  
S. Dunham

Lowbush blueberries (Vaccinium angustifolium Ait.) in three commercial fields were treated with 67.2 kg P/ha from triple super phosphate (TSP), monoammonium phosphate (MAP), or diammonium phosphate (DAP) and compared to a control in a randomized complete-block design with 12 blocks. Correction of P deficiency by fertilizers with different ratios of P to N was assessed by leaf nutrient concentrations. Samples of stems collected in July from three 0.03-m2 quadrats per treatment plot indicated MAP and DAP had no effect on dry weight of stem tissue, but increased average dry weight of leaf tissue. P and N Leaf concentrations were raised to higher levels by MAP and DAP than by TSP. TSP had no effect on leaf N concentrations but raised leaf P concentrations compared to controls. Stem length, flower buds per stem and flower bud density were increased by both MAP and DAP, but not TSP. MAP and DAP increased fruit yield by about 340 kg/ha compared to the control.


2021 ◽  
Author(s):  
Mengjiao Sun ◽  
Enqing Hou ◽  
Jiasen Wu ◽  
Jianqin Huang ◽  
Xingzhao Huang

Abstract Background: Soil nutrients play critical roles in regulating and improving the sustainable development of economic forests. Consequently, an elucidation of the spatial patterns and drivers of soil nutrients in these forests is fundamental to their management. For this study, we collected 314 composite soils at a 0-30 cm depth from a typical hickory plantation in Lin 'an, Zhejiang Province, China. We determined the concentrations of macronutrients (i.e., soil organic carbon, hydrolyzed nitrogen, available phosphorus, and available potassium) and micronutrients (i.e., iron, manganese, zinc, and copper.) of the soils. We employed random forest analysis to quantify the relative importance of soil-forming factors to predict the soil nutrient concentrations, which could then be extrapolated to the entire hickory region. Results: Random forest models explained 61%–88% of the variations in soil nutrient concentrations. The mean annual temperature and mean annual precipitation were the most important predictor of soil macronutrient and micronutrient concentrations. Moreover, parent material was another key predictor of soil available phosphorus and micronutrient concentrations. Mapping results demonstrated the importance of climate in controlling the spatial distribution of soil nutrient concentrations at finer scales, as well as the effect of parent material, topography, stand structure, and management measures of hickory plantations. Conclusions: Our study highlights the biotic factors, abiotic factors, and management factors control over soil macronutrient and micronutrient concentrations, which have significant implications for the sustainability of soil nutrients in forest plantations.


1995 ◽  
Vol 25 (1) ◽  
pp. 18-28 ◽  
Author(s):  
B.J. Hawkins ◽  
M. Davradou ◽  
D. Pier ◽  
R. Shortt

One-year-old seedlings of western red cedar (Thujapiicata Donn ex D.Don) and Douglas-fir (Pseudotsugamenziesii (Mirb.) Franco) were grown for one season in five nutrient treatments with nitrogen (N) supplied in solution at rates of 20, 100, or 250 mg•L−1 and phosphorus (P) supplied at rates of 4, 20, or 60 mg•L−1. Growth, onset of dormancy, frost hardiness on six dates, and foliar nutrient concentrations in autumn and spring were measured. Midwinter rates of net photosynthesis and transpiration were measured at air temperatures of 4, 7, and 11 °C in seedlings from all nutrient treatments. Recovery of net photosynthesis and transpiration in whole seedlings from the three N treatments was assessed at intervals for 28 days after the seedlings were frozen to −5, −15, and −25°C. Foliar N content differed significantly among nutrient treatments and was positively correlated with supply. Mitotic activity ceased earliest in plants with low N supply. Douglas-fir seedlings in the low-N treatment also ceased height growth earliest. These differences in growth had no significant correlation with frost hardiness. No consistent differences in frost hardiness among nutrient treatments were observed. Higher rates of N and P supply resulted in higher rates of winter net photosynthesis. Net photosynthesis was reduced dramatically by night frost, with greater damage occurring at lower temperatures. Net photosynthesis recovery occurred most quickly in seedlings with the midrate of N and P supply.


2018 ◽  
pp. 213-218 ◽  
Author(s):  
T. Gama ◽  
H.M. Wallace ◽  
S.J. Trueman ◽  
S.H. Bai

2020 ◽  
Vol 193 (1) ◽  
pp. 64-83 ◽  
Author(s):  
Flávia M Leme ◽  
Jürg Schönenberger ◽  
Yannick M Staedler ◽  
Simone P Teixeira

Abstract Species of Cannabaceae are wind pollinated, have inconspicuous and reduced flowers that are pistillate, staminate and apparently perfect on the same individual or on different individuals, with a single-whorled perianth and a pseudomonomerous gynoecium. Our objective is to understand the developmental processes that lead to such a reduced flower morphology and polygamy in Cannabis sativa, Celtis iguanaea and Trema micrantha. Floral buds and flowers were processed for surface, histological examinations and 3D reconstructions of vasculature. The single-whorled perianth is interpreted as a calyx because the organs are robust, have a broad base, an acute apex and quincuncial aestivation and are opposite the stamens. Petals are absent from inception. The dicliny is established at different development stages: stamens or carpels are absent from inception (Cannabis sativa), initiated and aborted during early (Trema micrantha, before sporo/gametogenesis) or late (Celtis iguanaea, after sporo/gametogenesis) development. Furthermore, in all species studied the carpels are congenitally united and the pseudomonomerous nature of the gynoecium is confirmed. Glandular trichomes are distributed on the bracts, sepals, anther connective and receptacle. Special floral features shared by species of Cannabaceae include precocious ovule development and sepals that are each vascularized by one bundle. The reduced flowers of Cannabaceae are the result of the absence from inception and/or abortion of organs and even of a whole whorl at different developmental stages, which were probably selected in response to pressures exerted by the similar pollination mechanism.


1986 ◽  
Vol 66 (4) ◽  
pp. 971-976 ◽  
Author(s):  
J. A. CUTCLIFFE

The effects of preplant soil applications of B, Mo and dolomitic limestone on yields and leaf tissue nutrient concentrations of Rally peas were investigated at five locations with initial soil pH levels of 5.1–5.9. Experiments were conducted for two consecutive growing seasons at each location. All treatments were preplant incorporated in a 2 × 2 × 2 factorial design with five replicates. Yields of shelled peas, adjusted to tenderometer 100, varied between experiments from 1.1 to 4.8 × 103 kg ha−1 and were not generally affected by B, Mo or lime at application rates of 2.0, 0.25 and 10 000 kg ha−1, respectively. Also, the micronutrient and lime treatments had no significant effects on germination, vine length, pea/vine ratio or maturity. Leaf tissue B, Mo and Mg concentrations were increased by the applications of B, Mo and dolomitic limestone, respectively. However, leaf tissue Ca concentration was not affected by the lime treatment. The results indicate that leaf tissue concentrations of 16–74 μg g−1 B, 0.04–1.34 μg g−1 Mo and 0.23–0.55% Mg were within the sufficiency range.Key words: Peas, boron, molybdenum, dolomitic limestone, yield, leaf tissue concentration


2018 ◽  
Vol 64 (10) ◽  
pp. 664-680 ◽  
Author(s):  
Maryanne Scott ◽  
Mamta Rani ◽  
Jamil Samsatly ◽  
Jean-Benoit Charron ◽  
Suha Jabaji

Plant endophytes are a group of microorganisms that reside asymptomatically within the healthy living tissue. The diversity and molecular and biochemical characterization of industrial hemp-associated endophytes have not been previously studied. This study explored the abundance and diversity of culturable endophytes residing in petioles, leaves, and seeds of three industrial hemp cultivars, and examined their biochemical attributes and antifungal potential. A total of 134 bacterial and 53 fungal strains were isolated from cultivars Anka, CRS-1, and Yvonne. The number of bacterial isolates was similarly distributed among the cultivars, with the majority recovered from petiole tissue. Most fungal strains originated from leaf tissue of cultivar Anka. Molecular and phylogenetic analyses grouped the endophytes into 18 bacterial and 13 fungal taxa, respectively. The most abundant bacterial genera were Pseudomonas, Pantoea, and Bacillus, and the fungal genera were Aureobasidium, Alternaria, and Cochliobolus. The presence of siderophores, cellulase production, and phosphorus solubilization were the main biochemical traits. In proof-of-concept experiments, re-inoculation of tomato roots with some endophytes confirmed their migration to aerial tissues of the plant. Taken together, this study demonstrates that industrial hemp harbours a diversity of microbial endophytes, some of which could be used in growth promotion and (or) in biological control designed experiments.


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