scholarly journals Antiproliferative Activity of Pyracantha and Paullinia Plant Extracts on Aggressive Breast and Hepatocellular Carcinoma Cells

2020 ◽  
Vol 10 (21) ◽  
pp. 7543
Author(s):  
Neha Kaushik ◽  
Hyeonyeong Yang ◽  
SungRyong Jeong ◽  
Nagendra Kumar Kaushik ◽  
Pradeep Bhartiya ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Breast Carcinoma ◽  
Cancer Cell ◽  
Plant Extracts ◽  
Anticancer Agents ◽  
Mammary Epithelial Cells ◽  
Cancer Cell Line ◽  
Mammary Epithelial ◽  
Carcinoma Cells ◽  
Hepatocellular Carcinoma Cells

In recent decades, the use of plants as a natural remedy has been widely applied in traditional medicine and the treatment of various diseases, including cancer. However, in order to confirm the potential benefits of anticancer drug development from natural sources, in-depth screening assessments are necessary. In the present study, we aimed to evaluate the cytotoxic effects of eight medicinal plants against breast carcinoma and hepatocellular carcinoma cell lines. Remarkably, among all the tested plant extracts, Pyracantha angustifolia and Paullinia cupana extracts showed maximum inhibition in the two cancer cell line models, as detected by cell viability assays, but not in normal mammary epithelial cells. Moreover, induction of cell cycle arrest was seen in both cancer cell models after treatment with extracts derived from the fruits of P. angustifolia and the seeds of P. cupana. Phytochemical and antioxidant analyses demonstrated the presence of high phenolic and flavonoid contents, including an increase in 2,2-diphenyl-1-picrylhydrazyl (DPPH) activity. The growth inhibition of human breast carcinoma and hepatocellular carcinoma cells mediated by both extracts appears to be associated with apoptosis and upregulated expression of pro-apoptotic genes (caspase-3, caspase-7, tumor suppressor protein-p53, cytochrome c, poly (ADP-ribose) polymerase, p53 upregulated modulator of apoptosis, and Bcl-2-associated X-protein). Together, these results indicate that P. angustifolia and P. cupana offer a promising approach for the development of anticancer agents. However, further detailed research is required to make these plants applicable for therapeutic use.

scholarly journals A quantitative chemotherapy genetic interaction map reveals new factors associated with PARP inhibitor resistance

2017 ◽  
Author(s):  
Hsien-Ming Hu ◽  
Xin Zhao ◽  
Swati Kaushik ◽  
Lilliane Robillard ◽  
Antoine Barthelet ◽  
...  
Keyword(s):  
Dna Repair ◽  
Cancer Cell ◽  
Mammary Epithelial Cells ◽  
Genetic Interaction ◽  
Parp Inhibitor ◽  
Cancer Cell Line ◽  
Parp Inhibitors ◽  
Mammary Epithelial ◽  
Interaction Map ◽  
The Impact

SUMMARYNearly every cancer patient is treated with chemotherapy yet our understanding of factors that dictate response and resistance to such agents remains limited. We report the generation of a quantitative chemical-genetic interaction map in human mammary epithelial cells that charts the impact of knockdown of 625 cancer and DNA repair related genes on sensitivity to 29 drugs, covering all classes of cancer chemotherapeutics. This quantitative map is predictive of interactions maintained in cancer cell lines and can be used to identify new cancer-associated DNA repair factors, predict cancer cell line responses to therapy and prioritize drug combinations. We identify that GPBP1 loss in breast and ovarian cancer confers resistance to cisplatin and PARP inhibitors through the regulation of genes involved in homologous recombination. This map may help navigate patient genomic data and optimize chemotherapeutic regimens by delineating factors involved in the response to specific types of DNA damage.

scholarly journals A New Approach: Treatment with Sodium Vanadate and Cisplatin Combination for Human Hepatocellular Carcinoma

Proceedings ◽  
2019 ◽  
Vol 40 (1) ◽  
pp. 14 ◽  
Author(s):  
Müge Gülcihan ÖNAL
Keyword(s):  
Hepatocellular Carcinoma ◽  
Hepg2 Cells ◽  
Hepatocellular Cancer ◽  
Cancer Cell Line ◽  
Carcinoma Cells ◽  
Sodium Vanadate ◽  
Liver Malignancy ◽  
New Approach ◽  
Hepatocellular Carcinoma Cells ◽  
Chemotherapy Drugs

The most common primary liver malignancy is hepatocellular carcinoma. Various chemotherapy drugs are used for treatment. These drugs have high toxicity to liver tissue. More effective, less toxic options should be preferred for treatment. The aim of this study is to investigate the cytotoxic effect of cisplatin and sodium vanadate combination (NaV) in hepatocellular carcinoma. The hepatocellular cancer cell line (HepG2) was used in this study. Increased concentration of cisplatin and a selected concentration of sodium vanadate were treated to HepG2 cells for 24- and 72-hours incubation times. The proliferation of HepG2 cells decreased with the combination of NaV and cisplatin. While cisplatin was effective in 10–4 M concentration in the proliferation of HepG2 cells, 10–4 M cisplatin with 10–3 M NaV in combination was less effective in the proliferation of HepG2 cells. According to these results, NaV and cisplatin show toxic effects on hepatocellular carcinoma cells. However, the combination of cisplatin and NaV has a less toxic effect than cisplatin and NaV on hepatocellular carcinoma cells.

Anti-Oxidant and Anticancerous Effect of Fomitopsis officinalis (Vill. ex Fr. Bond. et Sing) Mushroom on Hepatocellular Carcinoma Cells In Vitro through NF-kB Pathway

2021 ◽  
Vol 21 ◽  
Author(s):  
Nyamsambuu Altannavch ◽  
Xi Zhou ◽  
Md. Asaduzzaman Khan ◽  
Ashfaque Ahmed ◽  
Shinen Naranmandakh ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Lines ◽  
Cancer Cell ◽  
Annexin V ◽  
Carcinoma Cells ◽  
Stress Disorders ◽  
Phase Cell ◽  
Hepatocellular Carcinoma Cells ◽  
The Impact

Background: Fomitopsis officinalis (Vill. ex Fr. Bond. et Sing) is a medicinal mushroom, commonly called ‘Agarikon’, traditionally used to treat cough and asthma in the Mongolian population. Objective: The objective of this study was to examine the significance of biological activity of F. officinalis, and evaluate the antioxidant and anticancer activity of six fractions of F. officinalis residues (Fo1-powder form dissolved in ethanol, Fo2-petroleum ether residue, Fo3-chloroformic, Fo4-ethylacetate, Fo5-buthanolic, and Fo6-water-ethanolic) against hepatocellular carcinoma cells. Methods: We performed in vitro studies of cell proliferation and viability assay, annexin V-FITC/Propidium Iodide assay, and NF-kB signaling pathway by immunoblot analysis. Results: Our findings revealed that all six fractions/extracts have antioxidant activity, and somehow, they exert anticancerous effects against cancer cells. In cancerous cell lines (HepG2 and LO2), Fo3 chloroformic extract promoted the cancer cell apoptosis, cell viability, activated G2/M-phase cell cycle, and selectively induced NF-kB proteins, revealing itself as a novel antitumor extract. Conclusion: This study reports that Fo3-chloroformic extract is rich in antitumor activity; it was previously not investigated in cancer. To study the impact of F. officinalis among natural products to treat/prevent oxidative stress disorders or cancers, further examinations are needed. However, this study assessed only one extract, Fo3-chloroformic, which has a significant impact on cancer cell lines.

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