scholarly journals Enhancing the Anticancer Activity of Antrodia cinnamomea in Hepatocellular Carcinoma Cells via Cocultivation With Ginger: The Impact on Cancer Cell Survival Pathways

2018 ◽  
Vol 9 ◽  
Author(s):  
San-Yuan Chen ◽  
Ying-Ray Lee ◽  
Ming-Chia Hsieh ◽  
Hany A. Omar ◽  
Yen-Ni Teng ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Survival ◽  
Anticancer Activity ◽  
Cancer Cell ◽  
Carcinoma Cells ◽  
Hepatocellular Carcinoma Cells ◽  
Antrodia Cinnamomea ◽  
Survival Pathways ◽  
Cancer Cell Survival ◽  
The Impact

Anti-Oxidant and Anticancerous Effect of Fomitopsis officinalis (Vill. ex Fr. Bond. et Sing) Mushroom on Hepatocellular Carcinoma Cells In Vitro through NF-kB Pathway

2021 ◽  
Vol 21 ◽  
Author(s):  
Nyamsambuu Altannavch ◽  
Xi Zhou ◽  
Md. Asaduzzaman Khan ◽  
Ashfaque Ahmed ◽  
Shinen Naranmandakh ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Lines ◽  
Cancer Cell ◽  
Annexin V ◽  
Carcinoma Cells ◽  
Stress Disorders ◽  
Phase Cell ◽  
Hepatocellular Carcinoma Cells ◽  
The Impact

Background: Fomitopsis officinalis (Vill. ex Fr. Bond. et Sing) is a medicinal mushroom, commonly called ‘Agarikon’, traditionally used to treat cough and asthma in the Mongolian population. Objective: The objective of this study was to examine the significance of biological activity of F. officinalis, and evaluate the antioxidant and anticancer activity of six fractions of F. officinalis residues (Fo1-powder form dissolved in ethanol, Fo2-petroleum ether residue, Fo3-chloroformic, Fo4-ethylacetate, Fo5-buthanolic, and Fo6-water-ethanolic) against hepatocellular carcinoma cells. Methods: We performed in vitro studies of cell proliferation and viability assay, annexin V-FITC/Propidium Iodide assay, and NF-kB signaling pathway by immunoblot analysis. Results: Our findings revealed that all six fractions/extracts have antioxidant activity, and somehow, they exert anticancerous effects against cancer cells. In cancerous cell lines (HepG2 and LO2), Fo3 chloroformic extract promoted the cancer cell apoptosis, cell viability, activated G2/M-phase cell cycle, and selectively induced NF-kB proteins, revealing itself as a novel antitumor extract. Conclusion: This study reports that Fo3-chloroformic extract is rich in antitumor activity; it was previously not investigated in cancer. To study the impact of F. officinalis among natural products to treat/prevent oxidative stress disorders or cancers, further examinations are needed. However, this study assessed only one extract, Fo3-chloroformic, which has a significant impact on cancer cell lines.

scholarly journals LSD1 Promotes Prostate Cancer Cell Survival by Destabilizing FBXW7 at Post-Translational Level

2021 ◽  
Vol 10 ◽  
Author(s):  
Xu-ke Qin ◽  
Yang Du ◽  
Xiu-heng Liu ◽  
Lei Wang
Keyword(s):  
Prostate Cancer ◽  
Cell Survival ◽  
Cell Lines ◽  
Cancer Cell ◽  
Mrna Level ◽  
Knock Down ◽  
Catalytic Inhibitor ◽  
Cancer Cell Survival ◽  
Demethylase Activity ◽  
The Impact

Prostate cancer (PCa) is the most common cancer in men and the fifth leading cause of cancer death worldwide. Unfortunately, castration-resistant prostate cancer (CRPCa) is incurable with surgical treat and prone to drug resistance. Therefore, it is of great importance to find a new target for treatment. LSD1 is up-regulated in PCa and related with prognosis. The high-expression LSD1 has been shown to be a potential target for treatment and is widely studied for its demethylase-activity. However, its demethylation-independent function remains to be elusive in PCa. Recent study shows that LSD1 can destabilize cancer suppressor protein FBXW7 without demethylation-function. Hence, we hope to investigate the impact of non-canonical function of LSD1 on PCa cell survival. We over-expressed FBXW7 gene through plasmid vector in LNCaP and PC3 cell lines and the result shows that up-regulated FBXW7 can suppress the viability of PC cell through suppressing oncoproteins, such as c-MYC, NOTCH-1. After FBXW7 function experiment on PC cell, we knock-down LSD1 gene in the same kinds of cell lines. In western blot assay, we detected that down-regulation of LSD1 will cause the increasing of FBXW7 protein level and decreasing of its targeting oncoproteins. And mRNA level of FBXW7 did not change significantly after LSD1 knock-down, which means LSD1 may destabilize FBXW7 by protein-protein interactions. Moreover, exogenous wild type LSD1 and catalytically deficient mutant K661A both can abrogate previous effect of LSD1 knock-down. Consequently, LSD1 may promote PC cell survival by destabilizing FBXW7 without its demethylase-activity. Next, we compared two kinds inhibitors, and found that SP-2509 (Allosteric inhibitor) treatment suppress the cancer cell survival by blocking the LSD1–FBXW7 interaction, which is an effect that GSK-2879552 (catalytic inhibitor) cannot achieve. This work revealed a pivotal function of LSD1 in PCa, and indicated a new direction of LSD1 inhibitor research for PCa treatment.

scholarly journals ERK/AKT Inactivation and Apoptosis Induction Associate With Quetiapine-inhibited Cell Survival and Invasion in Hepatocellular Carcinoma Cells

In Vivo ◽  
2020 ◽  
Vol 34 (5) ◽  
pp. 2407-2417
Author(s):  
YEN-JU LEE ◽  
JING-GUNG CHUNG ◽  
ZHAO-LIN TAN ◽  
FEI-TING HSU ◽  
YU-CHANG LIU ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Survival ◽  
Carcinoma Cells ◽  
Apoptosis Induction ◽  
Hepatocellular Carcinoma Cells
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