scholarly journals Microbial Mediated Synthesis of Silver Nanoparticles by Lactobacillus Plantarum TA4 and its Antibacterial and Antioxidant Activity

2020 ◽  
Vol 10 (19) ◽  
pp. 6973
Author(s):  
Hidayat Mohd Yusof ◽  
Nor’Aini Abdul Rahman ◽  
Rosfarizan Mohamad ◽  
Uswatun Hasanah Zaidan
Keyword(s):  
Silver Nanoparticles ◽  
Lactobacillus Plantarum ◽  
Reduction Process ◽  
Antioxidant Property ◽  
Visual Observation ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Tem Analysis ◽  
Cell Biomass ◽  
Biosynthesized Agnps

The present study aimed to investigate the ability of Lactobacillus plantarum TA4 in tolerating Ag+ and its ability to produce silver nanoparticles (AgNPs). The biosynthesized AgNPs were characterized using UV–Visible spectroscopy (UV–Vis), dynamic light scattering (DLS), Fourier-transform infrared (FTIR), and high-resolution transmission electron microscope (HR-TEM). The cell biomass of L. plantarum TA4 demonstrated the ability to tolerate Ag+ at a concentration of 2 mM, followed by the formation of AgNPs. This was confirmed by the visual observation of color changes and a presence of maximum UV–Vis absorption centered at 429 nm. HR-TEM analysis revealed that the AgNPs were spherical with an average size of 14.0 ± 4.7 nm, while the SEM-EDX analysis detected that the particles were primarily located on the cell membrane of L. plantarum TA4. Further, DLS analysis revealed that the polydispersity index (PDI) value of biosynthesized AgNPs was 0.193, implying the monodispersed characteristic of NPs. Meanwhile, the FTIR study confirmed the involvement of functional groups from the cell biomass that involved in the reduction process. Moreover, biosynthesized AgNPs exhibited antibacterial activity against Gram-positive and Gram-negative pathogens in a concentration-dependent manner. Furthermore, the antioxidant property of biosynthesized AgNPs that was evaluated using the DPPH assay showed considerable antioxidant potential. Results from this study provide a sustainable and inexpensive method for the production of AgNPs.

scholarly journals Biosynthesis of silver nanoparticles using Haloferax sp. NRS1: image analysis, characterization, in vitro thrombolysis and cytotoxicity

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hend M. Tag ◽  
Amna A. Saddiq ◽  
Monagi Alkinani ◽  
Nashwa Hagagy
Keyword(s):  
Silver Nanoparticles ◽  
Image Analysis ◽  
Biological Activities ◽  
Positive Control ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Vis Spectroscopy ◽  
Negative Surface ◽  
Biosynthesized Agnps

AbstractHaloferax sp strain NRS1 (MT967913) was isolated from a solar saltern on the southern coast of the Red Sea, Jeddah, Saudi Arabia. The present study was designed for estimate the potential capacity of the Haloferax sp strain NRS1 to synthesize (silver nanoparticles) AgNPs. Biological activities such as thrombolysis and cytotoxicity of biosynthesized AgNPs were evaluated. The characterization of silver nanoparticles biosynthesized by Haloferax sp (Hfx-AgNPs) was analyzed using UV–vis spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FTIR). The dark brown color of the Hfx-AgNPs colloidal showed maximum absorbance at 458 nm. TEM image analysis revealed that the shape of the Hfx-AgNPs was spherical and a size range was 5.77- 73.14 nm. The XRD spectra showed a crystallographic plane of silver nanoparticles, with a crystalline size of 29.28 nm. The prominent FTIR peaks obtained at 3281, 1644 and 1250 cm− 1 identified the Functional groups involved in the reduction of silver ion reduction to AgNPs. Zeta potential results revealed a negative surface charge and stability of Hfx-AgNPs. Colloidal solution of Hfx-AgNPs with concentrations ranging from 3.125 to 100 μg/mL was used to determine its hemolytic activity. Less than 12.5 μg/mL of tested agent showed no hemolysis with high significant decrease compared with positive control, which confirms that Hfx-AgNPs are considered non-hemolytic (non-toxic) agents according to the ISO/TR 7405-1984(f) protocol. Thrombolysis activity of Hfx-AgNPs was observed in a concentration-dependent manner. Further, Hfx-AgNPs may be considered a promising lead compound for the pharmacological industry.

scholarly journals Biosynthesis of zinc oxide nanoparticles by cell-biomass and supernatant of Lactobacillus plantarum TA4 and its antibacterial and biocompatibility properties

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Hidayat Mohd Yusof ◽  
Nor’Aini Abdul Rahman ◽  
Rosfarizan Mohamad ◽  
Uswatun Hasanah Zaidan ◽  
Anjas Asmara Samsudin
Keyword(s):  
Lactobacillus Plantarum ◽  
Pathogenic Bacteria ◽  
Biological Synthesis ◽  
Hydroxyl Groups ◽  
Dependent Manner ◽  
Zno Nps ◽  
Concentration Dependent Manner ◽  
Stabilizing Agents ◽  
Cell Biomass ◽  
Vero Cell Line

AbstractThis study aims to utilize the cell-biomass (CB) and supernatant (CFS) of zinc-tolerant Lactobacillus plantarum TA4 as a prospective nanofactory to synthesize ZnO NPs. The surface plasmon resonance for the biosynthesized ZnO NPs-CFS and ZnO NPs-CB was 349 nm and 351 nm, respectively, thereby confirming the formation of ZnO NPs. The FTIR analysis revealed the presence of proteins, carboxyl, and hydroxyl groups on the surfaces of both the biosynthesized ZnO NPs that act as reducing and stabilizing agents. The DLS analysis revealed that the poly-dispersity indexes was less than 0.4 for both ZnO NPs. In addition, the HR-TEM micrographs of the biosynthesized ZnO NPs revealed a flower-like pattern for ZnO NPs-CFS and an irregular shape for ZnO NPs-CB with particles size of 291.1 and 191.8 nm, respectively. In this study, the biosynthesized ZnO NPs exhibited antibacterial activity against pathogenic bacteria in a concentration-dependent manner and showed biocompatibility with the Vero cell line at specific concentrations. Overall, CFS and CB of L. plantarum TA4 can potentially be used as a nanofactory for the biological synthesis of ZnO NPs.

scholarly journals Green Synthesis of Silver Nanoparticles by Cytobacillus firmus Isolated from the Stem Bark of Terminalia arjuna and Their Antimicrobial Activity

Biomolecules ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 259
Author(s):  
Sujesh Sudarsan ◽  
Madan Kumar Shankar ◽  
Anil Kumar Belagal Motatis ◽  
Sushmitha Shankar ◽  
Darshan Krishnappa ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Stem Bark ◽  
Visual Observation ◽  
Blast Disease ◽  
Terminalia Arjuna ◽  
Dependent Manner ◽  
Antibacterial And Antifungal Activities ◽  
Escherichia Coli Bacteria ◽  
Biosynthesized Agnps ◽  
Average Size

This work reports an eco-friendly synthesis of silver nanoparticles (AgNPs) using endophytic bacteria, Cytobacillus firmus isolated from the stem bark of Terminalia arjuna. The synthesis of AgNPs was confirmed by visual observation as a change in color of the bacterial solution impregnated with silver. Further, the morphology of the AgNPs, average size, and presence of elemental silver were characterized by UV–Visible spectroscopy, scanning electron microscopy, and dynamic light scattering spectroscopy. The roles of endophytic secondary metabolites in the metal reduction, stabilization, and capping of silver nanoparticles were studied by qualitative FTIR spectral peaks. The antimicrobial ability of AgNPs was evaluated against Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria and pearl millet blast disease-causing fungi (Magnoporthe grisea). The biosynthesized AgNPs showed good antibacterial and antifungal activities. AgNPs effectively inhibited the bacterial growth in a dose-dependent manner and presented as good antifungal agents towards the growth of Magnoporthe grisea.

scholarly journals Assessment of Antioxidant and Antimicrobial Activities of Silver Nanoparticles Biosynthesized by Haplophyllum Obtusifolium

2020 ◽  
Vol 8 (2) ◽  
pp. 94-98
Author(s):  
Mohammad Reza Rezaei ◽  
Ali Es-haghi ◽  
Parichehreh Yaghmaei ◽  
Maryam Ghobeh
Keyword(s):  
Antioxidant Activity ◽  
Silver Nanoparticles ◽  
Antimicrobial Agents ◽  
Antioxidant Activities ◽  
Antimicrobial Activities ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
The North ◽  
Chamber Temperature ◽  
Pharmaceutical Industries

Background: Plants comprise great antioxidant sources as a result of their redox and biochemical components, which are rich in secondary metabolites such as phenolic acids, flavonoids, and other constituents. Haplophyllum obtusifolium from polygonaceae is widely used for preventing and managing diabetes. This study investigated the antibacterial and antioxidant activities of silver nanoparticles (AgNPs) biosynthesized by H. obtusifolium. Methods: The aerial parts of H. obtusifolium were gathered from the north of Khorasan Razavi province, Iran and desiccated at the chamber temperature. The shoots were powdered by grinding, 5 g of the powder was mixed with 250 mL of deionized water, and the resultant blend was then filtered. Bactericidal properties and antioxidant activity of the nanoparticles were assessed using disk diffusion and DPPH (2, 2-diphenyl-1-picrylhydrazyl) tests, respectively. Results: The results of this study showed that the biosynthesized nanoparticles exhibited antibacterial activity against a gram-negative (Klebsiella pneumoniae) bacterium, but they had no effects on gram-positive Staphylococcus epidermidis. Antioxidant test results showed that these nanoparticles were capable of eliminating DPPH radicals in a concentration-dependent manner so that a more potent antioxidant activity was seen in higher concentrations of the nanoparticles. Conclusion: Our results suggested that H. obtusifolium can be used as a key source of antioxidants/ antimicrobial agents in food and pharmaceutical industries.

scholarly journals Biosynthesis, Characterization, and Antidermatophytic Activity of Silver Nanoparticles Using Raamphal Plant (Annona reticulata) Aqueous Leaves Extract

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
P. Shivakumar Singh ◽  
G. M. Vidyasagar
Keyword(s):  
Silver Nanoparticles ◽  
Chemical Compounds ◽  
Visual Observation ◽  
X Ray Diffraction ◽  
Tem Analysis ◽  
X Ray ◽  
Leaf Aqueous Extract ◽  
Vis Spectroscopy ◽  
Antidermatophytic Activity ◽  
Annona Reticulata

The present work investigated the biosynthesis of silver nanoparticles using Annona reticulata leaf aqueous extract. The biosynthesised silver nanoparticles were confirmed by visual observation and UV-Vis spectroscopy. Appearance of dark brown colour indicated the synthesis of silver in the reaction mixture. The silver nanoparticles were found to be spherical, rod, and triangular in shape with variable size ranging from 23.84 to 50.54 nm, as evident by X-ray diffraction studies, TEM. The X-ray diffraction studies, energy dispersive X-ray analysis, and TEM analysis indicate that the particles are crystalline in nature. The nanoparticles appeared to be associated with some chemical compounds which possess hydroxyl and carbonyl groups, confirmed by FTIR. This is the first and novel report of silver nanoparticles synthesised from Annona reticulata leaves extract and their antidermatophytic activity.

scholarly journals Antiproliferation Activity and Mechanism of c9, t11, c15-CLNA and t9, t11, c15-CLNA from Lactobacillus plantarum ZS2058 on Colon Cancer Cells

2020 ◽  
Author(s):  
Qing Ren ◽  
Bo Yang ◽  
Guangzhen Zhu ◽  
Shunyu Wang ◽  
Chengli Fu ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Lactobacillus Plantarum ◽  
Linolenic Acid ◽  
Antioxidant Defenses ◽  
Dependent Manner ◽  
Colon Cancer Cells ◽  
Alpha Linolenic Acid ◽  
Concentration Dependent Manner ◽  
Caspase 1

The probiotic bacterial strain Lactobacillus plantarum ZS2058 has been proved to manifest comprehensive functions, which were due to ability to synthesise conjugated fatty acids (CFAs). To investigate the specific functions of CFAs produced by this probiotic bacterium, α-linolenic acid was isomerized by Lactobacillus plantarum strain ZS2058, and two different conjugated α-linolenic acid (CLNA) isomers were successfully isolated. These isoforms, CLNA1 (c9, t11, c15-CLNA, purity 97.48%) and CLNA2 (c9, t11, t15-CLNA, purity 99.00%), both showed the ability to inhibit the growth of three types of colon cancer cells in a time- and concentration-dependent manner. In addition, the expression of MDA in Caco-2 cells was increased by CLNA1 or CLNA2, which indicated lipid peroxidation was related to the antiproliferation activity of CLNAs. Examination of the key protein of pyroptosis showed that CLNA1 induced the cleavage of caspase-1 and gasdermin-D, while CLNA2 induced the cleavage of caspase-4, 5 and gasdermin-D. The addition of relative inhibitors could alleviate the pyroptosis by CLNAs. CLNA1 and CLNA2 showed no effect on caspase-3, 7, 9 and PARP-1, which were key proteins associated with apoptosis. And no sub-diploid apoptotic peak appeared in the result of PI single staining test. In conclusion, CLNA1 activated caspase-1 and induced Caco-2 cell pyroptosis, whereas CLNA2 induced pyroptosis through the caspase-4/5-mediated pathway. The inhibition of Caco-2 cells by the two isomers was not related to apoptosis. This is the first report showing the ability of CLNAs to activate antioxidant defenses resulting in pyroptosis.

scholarly journals Biomedical Potentialities of Silver Nanoparticles for Clinical Multiple Drug-Resistant Acinetobacter baumannii

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Minghui Chen ◽  
Xiaoxu Yu ◽  
Qianyu Huo ◽  
Qin Yuan ◽  
Xue Li ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Clinical Problem ◽  
Multidrug Resistant ◽  
Multiple Drug ◽  
High Morbidity ◽  
Dependent Manner ◽  
Drug Resistant ◽  
Concentration Dependent Manner ◽  
Multiple Drug Resistant ◽  
New Strategy

Multidrug-resistant A. baumannii is increasingly recognized as a significant problem in hospitals and causes high morbidity and mortality. Here, we studied the antibacterial effects of AgNPs on clinically isolated multiple drug-resistant A. baumannii, and search for the potential antibacterial mechanism. Based on the results from the colony-forming unit (CFU) method, flow cytometry (FC), and a BrdU ELISA, we conclude that AgNPs can simultaneously induce apoptosis and inhibit new DNA synthesis in bacteria in a concentration-dependent manner. This study presents the first discussion of an antibacterial effect by AgNPs in clinically isolated, multidrug-resistant A. Baumannii and provides a new strategy for the use of silver nanoparticles in the multidrug-resistant A. Baumannii clinical problem.

scholarly journals Colloidal solution of silver nanoparticles for label-free colorimetric sensing of ammonia in aqueous solutions

2018 ◽  
Vol 9 ◽  
pp. 499-507 ◽  
Author(s):  
Alessandro Buccolieri ◽  
Antonio Serra ◽  
Gabriele Giancane ◽  
Daniela Manno
Keyword(s):  
Silver Nanoparticles ◽  
Aqueous Solutions ◽  
Morphological Analysis ◽  
Colloidal Solution ◽  
Ammonia Concentration ◽  
Dependent Manner ◽  
Label Free ◽  
Colorimetric Sensing ◽  
Concentration Dependent Manner ◽  
High Ammonia

Silver nanoparticles were synthesized in the presence of saccharides and ammonia (NH3) in the concentration range from 10−2 to 103 ppm to develop an optical sensor for NH3 in aqueous solutions. Ammonia affects the features of the nanoparticles obtained in a concentration-dependent manner as determined by UV–vis absorption analysis and TEM observations. Structural and morphological analysis provides the basis for the production of a colorimetric label-free sensor for ammonia. Overall, surface plasmon resonance increases when ammonia concentration rises, although the functional trend is not the same over the entire investigated ammonia concentration range. Three different ranges have been identified: very low ammonia concentrations from 0.01 to 0.2 ppm, high ammonia concentrations from 20 to 350 ppm and, most importantly, the intermediate or physiological range of ammonia from 0.5 to 10 ppm.

scholarly journals Antithrombotic, Antihemolytic Activities and Protein Conjugation Properties of Silver Nanoparticles Synthesized from Turbinaria ornata

2021 ◽  
Vol 33 (8) ◽  
pp. 1736-1742
Author(s):  
Amalan Venkatesan ◽  
Jose Vinoth Raja Antony Samy ◽  
Rajeswari Ranga Anantha Sayanam ◽  
Jayaprakash Rajendran ◽  
Vijayakumar Natesan
Keyword(s):  
Silver Nanoparticles ◽  
Cardiovascular Diseases ◽  
Blood Clot ◽  
Clot Lysis ◽  
Dependent Manner ◽  
Protein Conjugation ◽  
Clotting Factors ◽  
Concentration Dependent Manner ◽  
Tertiary Structures ◽  
Turbinaria Ornata

Green synthesis of silver nanoparticles synthesized from Turbinaria ornata weed extract was carried out. Because these seaweeds are largely unexplored and contain botanical molecules that promote bimolecular reduction. In this study, silver nanoparticles (AgNPs) cause a rise in the accumulation of clotting factors, platelets and procoagulant activity, both of which lead to thrombotic and hemolytic diseases. The silver nanoparticles were initially characterized using spectroscopic analysis to validate the biomolecules of Turbinaria ornata involved in nanoparticle reduction. A detailed study on the effectiveness of silver nanoparticles on antithrombolytic activity in adult blood samples confirms the interaction of nanoparticles with platelets and blood vessels, which is significant in the production of thrombosis and cardiovascular diseases. The antihemolytic activity was tested to assess the percentage of blood clot lysis in order to confirm the ability to scavenge hydrogen peroxide in a concentration dependent manner. The protein conjugation and binding activities of major, secondary, and tertiary structures were also investigated in silico. Thus, Turbinaria ornata seaweeds with reduced silver nanoparticles demonstrated increased antithrombotic and antihemolytic activities, suggested that they could be used as novel therapeutics for cardiovascular diseases.

scholarly journals Biogenic Nanosilver Mediated by Coir, Medicinal Plant Extracts and their Antimicrobial Validation

CORD ◽  
2017 ◽  
Vol 33 (2) ◽  
pp. 18
Author(s):  
Geethanjali Kanagaraju
Keyword(s):  
Staphylococcus Aureus ◽  
Particle Size ◽  
Silver Nanoparticles ◽  
Plant Extracts ◽  
Research Work ◽  
Antimicrobial Activities ◽  
Tem Analysis ◽  
Sem And Tem ◽  
Medicinal Plant Extracts ◽  
Biosynthesized Agnps

Knowing the magnitude of therapeutic plants such as Micrococca mercurialis (MM), Abutilon palmeri (AP) and Callistemon citrinus (CC), we also planned to extend our research work on Cocusnucifera fibers (CF) and dust (CD) as it has copious medicinal properties. The current study deals with the green synthesis of silver nanoparticles (AgNPs) from fresh aqueous extracts and AgNO3 solution. The biogenic conversion of silver ion to silver is relatively expeditious at two different time intervals and pH. The isolated  silver nanoparticles (AgNPs) from the bio extracts were identified initially by scrutinizing the colour variations. The biosynthesized AgNPs were characterized by UV, XRD, Laser Diffraction Particle size Analyser, fluorescence spectroscopy, SEM and TEM. UV absorbance at 435-460nm for silver nanoparticles was observed for the above extracts. The XRD pattern of all extracts showed the distinctive Bragg peaks of face centre cubic (fcc) crystalline system available in nature. SEM and TEM analysis of the silver nanoparticles indicated that the particle size was in the range of 2-100nm with polygonal and spherical shapes. The biosynthesized AgNPs were evaluated for antimicrobial activities. The CFAgNPs showed an efficient antibacterial activity at lower concentration (25mg/ml) against Pseudomonas demolytica followed by Staphylococcus aureus and Escherichia coli. Similarly all plant extracts have better activity against fungal strains. It is concluded that the biogenic blend of AgNPs is simple, extremely rapid, beneficial, eco-friendly and more stable without any toxic effects. Of these, CFAgNps may be used for the preparation of antibacterial groupings against Pseudomonas demolytica and Staphylococcus aureus.

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