scholarly journals Development and Application of a Motion Analysis Protocol for the Kinematic Evaluation of Basic and Functional Hand and Finger Movements Using Motion Capture in a Clinical Setting—A Repeatability Study

2020 ◽  
Vol 10 (18) ◽  
pp. 6436
Author(s):  
Gabriella Fischer ◽  
Diana Jermann ◽  
Renate List ◽  
Lisa Reissner ◽  
Maurizio Calcagni
Keyword(s):  
Motion Analysis ◽  
Data Interpretation ◽  
Clinical Applications ◽  
Finger Joint ◽  
Functional Assessments ◽  
Standard Values ◽  
Kinematic Evaluation ◽  
Simultaneous Assessment ◽  
Analysis Protocol

The purpose of this study was to develop a motion analysis protocol that allows the simultaneous assessment of all hand and finger joint movements. The objective was to demonstrate repeatability for future clinical applications in functional assessments. This study includes selection of marker positions, movement tasks, kinematic approaches and a comparison of the two most commonly used finger marker sets. By using a test–retest measurement of the range of motion in twenty healthy volunteers, the repeatability of the developed protocol was validated. Estimated errors of the presented method ranged from 1.2° to 6.4°. Finger joint angles derived from the marker set with two markers per segment showed better repeatability (3.7°) than with markers located on the joints (5.1°). Given the high repeatability found, the presented method appears to be suitable for clinical applications. For the fingers, measurement repeatability can be improved by using at least two markers per segment. Within this study, advanced kinematic approaches, such as functional determination of joint centers and axes, are applied to the analysis of hand movements. The provided standard values and estimate of the minimal detectable differences provide a valuable basis for meaningful data interpretation and may be used for future comparison with other protocols.

Reduction of Potassium in Kidney Proximal Tubules to Aid in Electron Probe X-Ray Microanalysis of Calcium

1985 ◽  
Vol 43 ◽  
pp. 474-475
Author(s):  
A. LeFurgey ◽  
P. Ingram ◽  
L.J. Mandel
Keyword(s):  
Smooth Muscle ◽  
Proximal Tubule ◽  
Electron Probe ◽  
Clinical Applications ◽  
Proximal Tubules ◽  
Rabbit Kidney ◽  
Target Cells ◽  
X Ray ◽  
Freeze Dried

For quantitative determination of subcellular Ca distribution by electron probe x-ray microanalysis, decreasing (and/or eliminating) the K content of the cell maximizes the ability to accurately separate the overlapping K Kß and Ca Kα peaks in the x-ray spectra. For example, rubidium has been effectively substituted for potassium in smooth muscle cells, thus giving an improvement in calcium measurements. Ouabain, a cardiac glycoside widely used in experimental and clinical applications, inhibits Na-K ATPase at the cell membrane and thus alters the cytoplasmic ion (Na,K) content of target cells. In epithelial cells primarily involved in active transport, such as the proximal tubule of the rabbit kidney, ouabain rapidly (t1/2= 2 mins) causes a decrease2 in intracellular K, but does not change intracellular total or free Ca for up to 30 mins. In the present study we have taken advantage of this effect of ouabain to determine the mitochondrial and cytoplasmic Ca content in freeze-dried cryosections of kidney proximal tubule by electron probe x-ray microanalysis.

The determination of urea in soil extracts and related samples—a review

Soil Research ◽  
2004 ◽  
Vol 42 (7) ◽  
pp. 709 ◽  
Author(s):  
David F. Lambert ◽  
John E. Sherwood ◽  
Paul S. Francis
Keyword(s):  
Enzymatic Hydrolysis ◽  
Flow Injection Analysis ◽  
Acidic Solution ◽  
Clinical Applications ◽  
Urease Inhibitors ◽  
Viable Option ◽  
Soil Extracts ◽  
Diacetyl Monoxime ◽  
Hydrolysis Of

Although the dominant methods for the determination of urea in clinical applications incorporate selective enzymatic hydrolysis of urea, the determination of urea in soil extracts is complicated by the presence of urease inhibitors. The spectrophotometric determination of urea with an acidic solution diacetyl monoxime and semicarbazide is a viable option but traditional manual procedures are time-consuming. New variations on these procedures, based on microplates or flow-injection analysis methodologies, allow a far greater number of samples to be analysed with high precision and sensitivity.

scholarly journals Protocol for examining and eliminating base editor-induced genome-wide and transcriptome-wide off-target mutations

2021 ◽  
Author(s):  
Lijie Wang ◽  
Wei Xue ◽  
Hongxia Zhang ◽  
Runze Gao ◽  
Houyuan Qiu ◽  
...  
Keyword(s):  
Clinical Applications ◽  
Cytidine Deaminases ◽  
Base Editing ◽  
Plasmid Construction ◽  
Background Levels ◽  
Genome Wide ◽  
Deoxycytidine Deaminase

Abstract Fusion of CRISPR-Cas9 with cytidine deaminases leads to base editors (BEs) for programmable C-to-T editing, which holds potentials in clinical applications but suffers from off-target (OT) mutations. Here, we applied a cleavable deoxycytidine deaminase inhibitor (dCDI) domain to construct a transformer BE (tBE) system that induces efficient editing with only background levels of genome-wide and transcriptome-wide OT mutations. This step-by-step protocol describes the plasmid construction of tBE system, determination of genome/transcriptome-wide OT mutations and tBE-mediated base editing in vivo.

scholarly journals Assessment of dielectric charging in micro-electro-mechanical system capacitive switches

2013 ◽  
Vol 26 (3) ◽  
pp. 239-245
Author(s):  
M. Koutsoureli ◽  
L. Michalas ◽  
G. Papaioannou
Keyword(s):  
Mechanical System ◽  
Material Properties ◽  
Micro Electro Mechanical System ◽  
Capacitive Switches ◽  
Metal Insulator ◽  
Dielectric Charging ◽  
Metal Insulator Metal ◽  
Simultaneous Assessment

The assessment of dielectric charging in MEMS capacitive switches is investigated. The information can be obtained only from simultaneous assessment of Metal-Insulator-Metal capacitance and MEMS capacitive switches the former allowing the determination of material properties and the latter of the device.

scholarly journals DETERMINATION OF FOOT-PLATE SPACING FOR SWIVEL WALKERS BY AN OPTIMIZATION METHOD

2010 ◽  
Vol 22 (03) ◽  
pp. 213-221
Author(s):  
Chih-Hsiu Cheng ◽  
Liang-Wey Chang ◽  
Kwan-Hwa Lin
Keyword(s):  
Theoretical Work ◽  
Optimization Method ◽  
Clinical Applications ◽  
The Body ◽  
Coronal Plane ◽  
Biped Locomotion ◽  
Optimal Design Problem ◽  
Plate Spacing ◽  
Feasible Range

Swivel walkers have been useful devices in ambulation for many young paraplegic patients for being advantageous in providing reliable stability, easy handling, and hands-free walking. The placement of the foot-plates on swivel walkers affects the gait efficiency. However, the determination of the foot-plate spacing has been purely empirical and no theoretical work has been attempted before. This study aimed to develop a dynamic model of the swivel walker in the coronal plane to formulate an optimal design problem such that the energy loss due to impact could be computed and minimized within a feasible range of the coronal-plane movement. Children of heights from 0.75 to 1.45 m and weights from 15 to 45 kg fitted with the conventional swivel walkers were simulated. The results indicated that the range of the foot-plate spacing was roughly between 1/4 and 1/6 of the body heights. A regression formula was also derived to estimate foot-plate spacing with respect to the heights and weights of the simulated subjects for clinical applications. We conclude that the theoretical framework not only builds a foundation to determine the foot-plate spacing, but also reveals the dynamic behavior of the swivel walkers in the coronal plane. The results could be applied to the design of other biped locomotion systems.

scholarly journals The Preparation, Determination of a Flexible Complex Liposome Co-Loaded with Cabazitaxel and β-Elemene, and Animal Pharmacodynamics on Paclitaxel-Resistant Lung Adenocarcinoma

Molecules ◽  
2019 ◽  
Vol 24 (9) ◽  
pp. 1697 ◽  
Author(s):  
Yi-Ying Zeng ◽  
Yi-Jun Zeng ◽  
Na-Na Zhang ◽  
Chen-Xi Li ◽  
Tian Xie ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Lung Adenocarcinoma ◽  
Malignant Tumors ◽  
Clinical Applications ◽  
Paclitaxel Resistance ◽  
Release Rates ◽  
Dialysis Method ◽  
Difficult Challenge ◽  
Weibull Equation

Paclitaxel is highly effective at killing many malignant tumors; however, the development of drug resistance is common in clinical applications. The issue of overcoming paclitaxel resistance is a difficult challenge at present. In this study, we developed nano drugs to treat paclitaxel-resistant lung adenocarcinoma. We selected cabazitaxel and β-elemene, which have fewer issues with drug resistance, and successfully prepared cabazitaxel liposome, β-elemene liposome and cabazitaxel-β-elemene complex liposome with good flexibility. The encapsulation efficiencies of cabazitaxel and β-elemene in these liposomes were detected by precipitation microfiltration and microfiltration centrifugation methods, respectively. Their encapsulation efficiencies were all above 95%. The release rates were detected by a dialysis method. The release profiles of cabazitaxel and β-elemene in these liposomes conformed to the Weibull equation. The release of cabazitaxel and β-elemene in the complex liposome were almost synchronous. The pharmacodynamics study showed that cabazitaxel flexible liposome and β-elemene flexible liposome were relatively good at overcoming paclitaxel resistance on paclitaxel-resistant lung adenocarcinoma. As the flexible complex liposome, the dosage of cabazitaxel could be reduced to 25% that of the cabazitaxel injection while retaining a similar therapeutic effect. It showed that β-elemene can replace some of the cabazitaxel, allowing the dosage of cabazitaxel to be reduced, thereby reducing the drug toxicity.

scholarly journals Optoelectronic measurement of wrist movements in various casts and orthoses used in scaphoid fractures

2019 ◽  
Vol 44 (6) ◽  
pp. 607-613
Author(s):  
Andrew Waton ◽  
Sarah Forrest ◽  
Gemma M. Whatling
Keyword(s):  
Healthy Volunteers ◽  
Motion Analysis ◽  
Wrist Motion ◽  
Scaphoid Fractures ◽  
Analysis Protocol ◽  
Optoelectronic Measurement

We developed an optoelectronic motion analysis protocol to measure anatomical and functional ranges of wrist motion in Colles’-type and scaphoid-type splints and casts. The protocol was used to study the restriction of wrist motion in casts and splints in ten healthy volunteers. Scaphoid-type casts were no more restrictive to wrist motion than Colles’-type casts, but casts were significantly more restrictive than removable splints. Removable splints were more restrictive than no immobilization. Results suggest there is no benefit in using scaphoid-type casts rather than Colles’-type casts to reduce wrist motion.

Automated Determination of Free Amino Groups in Serum and Plasma Using 2,4,6-Trinitrobenzene Sulfonate

1969 ◽  
Vol 15 (9) ◽  
pp. 891-901 ◽  
Author(s):  
D W Palmer ◽  
T Peters
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Clinical Applications ◽  
Amino Groups ◽  
Automated Method ◽  
Total Free Amino Acids ◽  
Colored Complexes ◽  
Automated Determination

Abstract A simple automated method is described for determining the level of total free amino acids in the blood. The method utilizes the AutoAnalyzer, and is based on the formation of colored complexes by uniting free amino groups with 2,4,6-trinitrobenzene sulfonate (TNBS). Proteins do not interfere because the free amino acids are first separated by dialysis. Characteristics of the reaction and potential clinical applications of the procedure are discussed.

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