scholarly journals The Effect of Polyphenolics in Extracts from Natural Materials on Metabolic Activity of Metastatic Melanoma WM-266-4 Cells

2020 ◽  
Vol 10 (10) ◽  
pp. 3499 ◽  
Author(s):  
Taja Žitek ◽  
Barbara Dariš ◽  
Matjaž Finšgar ◽  
Željko Knez ◽  
Dragana Bjelić ◽  
...  

The importance of natural crops in medicine and pharmacy is growing. Beside bioactive compounds used directly as therapeutic agents, there are also raw materials used for drug synthesis or as a basic model for new biologically active compounds. In this paper, the optimum conditions for material extraction of Curcuma longa, Lycium barbarum, Equisetum arvense, Vitis vinifera, and Rosmarinus officinalis were investigated to achieve high antioxidant levels. The main aim of this study was to verify the correlation between the content of antioxidants, proanthocyanidins and total phenolic substances for certain extracts from the raw materials (Curcuma longa, Lycium barbarum, Equisetum arvense, Vitis vinifera and Rosmarinus officinalis) and the reduction of the metabolic activity of skin cancer cells.

Author(s):  
Chernichkina A.D.

A large number of biologically active substances, organic acids, tannins, and pectin substances were detected during the study of fruits, leaves, and pulp of the fruits of mountain Ash. The content of these substances in leaves and pulp will significantly expand the arsenal of medicinal plant raw materials used. Given the wide distribution of mountain Ash in the territory of the Russian Federation, harvesting leaves from the same plants after harvesting the fruit and using pulp will make it possible to obtain new phytopreparations.


2021 ◽  
pp. 17-20
Author(s):  
Н.Л. Девочкина ◽  
С.В. Мукиенко ◽  
Л.Г. Дугуниева

Представлена информация по изучению технологических особенностей культивирования нового для российского продовольственного грибного рынка вида дереворазрушающего гриба – шиитаке (Lentinus edodes (Berk.) Sin). Шиитаке – один из наиболее перспективных для промышленного культивирования видов съедобных грибов, обладающих лечебными свойствами, занимает второе место в мировом производстве съедобных грибов. Работа по поиску материалов, используемых в качестве добавок к основному исходному материалу субстрата и способствующих созданию благоприятного водно-воздушного и кислотного режима, повышению питательности приготовленного субстрата, ускорению процесса вегетативного роста мицелия в субстрате, актуальна для грибоводческой практики. Цель исследований – разработать технологический процесс полного цикла культивирования шиитаке и установить его оптимальные параметры в производственных условиях ООО «Апрель». Исследования, испытания и разработку технологии полного цикла выращивания шиитаке проводили в лаборатории грибоводства ВНИИО – филиале ФГБНУ ФНЦО и на базе действующего предприятия по выращиванию дереворазрушающих грибов ООО «Апрель» с 2019 по 2020 год. Технология приготовления субстрата и выращивание плодовых тел шиитаке аналогична технологии производства вешенки. Изучено влияние состава субстрата на урожайность шиитаке в вариантах: 1. Опилки дуба (80%) + опилки березы (10%) + минеральные добавки (10%); 2. Опилки дуба (80%) + опилки березы (5%) + лузга семян подсолнечника, отруби пшеничные (5%) + минеральные добавки (10%). Использован штамм шиитаке 0912. В результате апробации разработанного технологического процесса полного цикла культивирования шиитаке в производственных условиях было установлено, что оптимальная продолжительность периода выращивания шиитаке и получение урожая в камерах выращивания составляет в среднем 53–68 суток. Важнейшая составляющая технологического процесса культивирования шиитаке – приготовление субстрата, подбор исходных материалов и биологически активных добавок, обеспечивающих его высокую питательность (содержание общего азота – 0,8–1,2% и более) и возможность получения урожая более 30–35% от массы субстрата с высокой экономической эффективностью от 290% (рентабельность производства). The article presents information on the study of technological features of cultivation of a new type of wood-destroying mushroom – shiitake (Lentinus edodes(Berk.) Sin), which is new for the Russian food mushroom market. Shiitake – one of the most promising types of edible mushrooms for industrial cultivation, which have medicinal properties, occupies the second place in the world production of edible mushrooms. The work on the search for materials used as additives to the main source material of the substrate and contributing to the creation of a favorable water-air and acid regime, increasing the nutritional value of the prepared substrate, accelerating the process of vegetative growth of mycelium in the substrate is relevant for mushroom growing practice. The purpose of the research – to develop the technological process of the full cycle of shiitake cultivation and to establish its optimal parameters in the production conditions of LLC April. Research, testing and development of the technology of the full cycle of shiitake cultivation were carried out in the laboratory of mushroom growing of ARRIVG – branch of Federal Scientific Centre of Vegetable Growing and on the basis of the existing enterprise for growing wood-destroying mushrooms LLC April from 2019 to 2020. The technology of preparing the substrate and growing shiitake fruit bodies is similar to the technology of producing oyster mushrooms. The influence of the substrate composition on the shiitake yield in the variants was studied: 1. Oak sawdust (80%) + birch sawdust (10%) + mineral additives (10%); 2. Oak sawdust (80%) + birch sawdust (5%) + sunflower seed husk, wheat bran (5%) + mineral additives (10%). The shiitake 0912 strain was used. As a result of testing the developed technological process of the full cycle of shiitake cultivation in production conditions, it was found that the optimal duration of the shiitake growing period and the harvest in the growing chambers is on average 53–68 days. The most important component of the technological process of shiitake cultivation is the preparation of the substrate, the selection of raw materials and biologically active additives that ensure its high nutritional value (total nitrogen content of 0.8–1.2% or more) and the possibility of obtaining a crop of more than 30–35% of the substrate weight with high economic efficiency of 290% (profitability of production).


Author(s):  
Roland Kopaliani ◽  
Temur Gvinianidze ◽  
Rezo Jabnidze

This paper dwells on the uvological characteristics of cultivar Vitis vinifera L. ‘Red Aladasturi’ grape raw materials growing in the viticulture and winemaking zone of Imereti (Georgia), as well as biologically active compounds and antioxidant activity of hydrophilic extracts and liquid concentrates of its solid matters (stone and skin). Research also covered hydrophilic extracts of grape skin and stone thickened by the vacuum of ‘Red Aladasturi’ grapes raw materials, as well as the concentrates produced from their composition. For research, there were used gravimetric, extractive, spectral and chromatographic methods. We processed samples of grapes raw materials according to the following pattern: identifying qualitative indicators of grapes raw materials; passing grapes raw materials through the DMCSI-type grape clustercomb divider; pressing-out the combless must in a basket press and separation of juice; vacuum sublimation drying of juiceless sweet pomace with an initial moisture content of 45–65% to a final moisture content of 9–10%; separation of the ‘Red Aladasturi’ cultivar’s skin and stone dried to the moisture content of 9–10%, using tea sorting machine designed by G. Lominadze; crushing separately the skin and stone in a micro-mill (TP2 Hammer Mill) until the fraction of 50–100 µm. we have blended the obtained grape-stone ethanol and fluid extracts containing 74–75% of solid maters at an equal ratio (1:1:1) and assessed biologically active compounds and antioxidant activity in this composition. It has been established that the bio-flavanoid liquid concentrate ‘Red Aladasturi’ is strong antioxidant  (55.31–57.45%), and one tablespoon or 8–9 ml of it contains 110–127 mg of flavanoids, which is 105–110% of a full day of rations per person per day.


Antioxidants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1848
Author(s):  
Jolanta Pytko-Polończyk ◽  
Magdalena Stawarz-Janeczek ◽  
Agata Kryczyk-Poprawa ◽  
Bożena Muszyńska

Antioxidant-rich natural raw materials have been used for thousands of years in traditional medicine. In the past decade, there has been increasing interest in naturotherapy, which is a practice of using products with a natural origin. Natural products can be effective in the treatment and prevention of oral and dental diseases, among others. Such raw materials used in dentistry are characterized by antioxidant, anti-inflammatory, antibacterial, antiviral, antiedematous, astringent, anticoagulant, dehydrating, vitaminizing, and—above all—regenerative properties. Reports have shown that a relationship exists between oral diseases and the qualitative and quantitative composition of the microbiota colonizing the oral cavity. This review aimed to analyze the studies focusing on the microbiome colonizing the oral cavity in the context of using natural raw materials especially herbs, plant extracts, and isolated biologically active compounds as agents in the prevention and treatment of oral and periodontal diseases such as dental caries as well as mucosal changes associated with salivary secretion disorder. The present work discusses selected plant ingredients exhibiting an antioxidant activity with potential for the treatment of selected oral cavity and periodontal diseases.


2018 ◽  
pp. 199-207 ◽  
Author(s):  
Залина (Zalina) Маирбековна (Mairbekovna) Тохсырова (Tokhsirova) ◽  
Иван (Ivan) Викторович (Viktorovich) Попов (Popov) ◽  
Ольга (Ol'ga) Ивановна (Ivanovna) Попова (Popova)

The object of the study was the leaves and shoots of rosemary medicinal (Rosmarinus officinalis L.), introduced for 9 years in the conditions of Caucasian Mineral Waters (North Caucasus) in the Botanical garden of the Pyatigorsk medical and pharmaceutical Institute in the open ground with shelter for the winter. Preliminary identification of phenolic compounds in the extracts from leaves and shoots of rosemary were carried out by thin-layer chromatography (TLC), and found gallic acid, rosmarinic acid, quercetin and apigenin.The method of high-performance liquid chromatography (HPLC) at wavelengths of 254 and 330 nm in leaves and shoots rosemary drug found 10 compounds: flavonoids: catechin, epicatechin, quercetin, apigenin; phenolcarboxylic acids: caffeic, gallic, chlorogenic, ferulic, rosmarinic as well as ascorbic acid. The content of rosmarinic acid in terms of dry raw materials in rosemary leaves was 0.181–0.184%; in shoots 0.062–0.064%.The area of the Northern Caucasus can be considered as a promising area for cultivation of medicinal plants – rosemary medicinal (Rosmarinus officinalis) – for the production of medicinal plant raw materials with a high content of biologically active substances.


Author(s):  
Crina Carmen MURESAN ◽  
Anca FARCAS ◽  
Simona MAN ◽  
Ramona SUHAROSCHI ◽  
Romina Alina VLAIC

The present study focuses on the influence of adding mushroom powder on a certain assortment of pasta. The research comprises two major directions: the study of the raw materials used to produce pasta from a compositional point of view and the quantification of some biologically active compounds of interest; emphasizing the potential of using Boletus edulis mushroom powder in the composition of pasta and evaluating the quality of the product from a nutritional and sensorial point of view. For this purpose, two types of pasta have been created, with different percentages of mushroom, 10% and 20%, but also a blank sample obtained in the same conditions, but without mushroom powder. To achieve the goal the following analyzes were conducted: proteins, total polyphenols, antioxidant activity, fat, humidity, ashes, acidity, increase in volume of the boiled pasta and customers’ preferences.By using sensory analysis has been established that the consumers preferred pasta enriched with 10% mushroom powder. In conclusion, the addition of mushrooms flour assures an enhancement of the nutritional value, as well as of the organoleptic characteristics of the final product.


2021 ◽  
Vol 9 (11) ◽  
pp. 2391
Author(s):  
Zdeněk Kodeš ◽  
Maria Vrublevskaya ◽  
Markéta Kulišová ◽  
Petr Jaroš ◽  
Martina Paldrychová ◽  
...  

Vitis vinifera canes are waste material of grapevine pruning and thus represent cheap source of high-value polyphenols. In view of the fact that resistance of many pathogenic microorganisms to antibiotics is a growing problem, the antimicrobial activity of plant polyphenols is studied as one of the possible approaches. We have investigated the total phenolic content, composition, antioxidant activity, and antifungal activity against Candida biofilm of an extract from winter canes and a commercially available extract from blue grapes. Light microscopy and confocal microscopy imaging as well as crystal violet staining were used to quantify and visualize the biofilm. We found a decrease in cell adhesion to the surface depending on the concentration of resveratrol in the cane extract. The biofilm formation was observed as metabolic activity of Candida albicans, Candida parapsilosis and Candida krusei biofilm cells and the minimum biofilm inhibitory concentrations were determined. The highest inhibition of metabolic activity was observed in Candida albicans biofilm after treatment with the cane extract (30 mg/L) and blue grape extract (50 mg/L). The composition of cane extract was analyzed and found to be comparatively different from blue grape extract. In addition, the content of total phenolic groups in cane extract was three-times higher (12.75 gGA/L). The results showed that cane extract was more effective in preventing biofilm formation than blue grape extract and winter canes have proven to be a potential source of polyphenols for antimicrobial and antibiofilm treatment.


Author(s):  
T. I. Bokova ◽  
I. V. Vasiltsova ◽  
Yu. I. Koval

The results of assessing the detoxification ability of raw materials of natural origin – propolis, buds and birch leaves (Betula pendula), buds and pine needles (Pinus sylvestris) in relation to lead and cadmium ions in in vitro experiments using the I. method. G. Mokhnacheva. The plant raw materials used in the experiment contain a large number of biologically active substances, including flavonoids (propolis, buds and birch leaves, pine buds), vitamin C (pine needles), which provide a detoxifying effect. Mass concentration of heavy metals was measured by inversion voltammetry on a TA-07 analyzer. It has been shown that raw materials of natural origin have a positive effect on the decrease in the content of lead and cadmium in model solutions. The use of pine needles caused a decrease in the level of lead to 78.4 %, pine buds to 67.4, birch leaves to 62.4, birch buds to 59.4, propolis to 57.6 %. The concentration of cadmium ions in the solution decreased under the influence of pine buds to 25.9 %, birch leaves to 19.3, propolis to 17.7 pine needles to 17.1, birch buds to 14.8 %. It has been established that the most effective lead detoxifiers are propolis, pine buds and needles; cadmium – buds of pine.


Molecules ◽  
2020 ◽  
Vol 25 (23) ◽  
pp. 5574
Author(s):  
Tomasz Tarko ◽  
Aleksandra Duda-Chodak ◽  
Agata Soszka

The content of polyphenols (total phenolic content (TPC)) and the antioxidant activity (AOX) of food products depend on the raw materials used and the technological processes in operation, but transformations of these compounds in the digestive tract are very important. The aim of this study was to determine the TPC, profile of polyphenols, and AOX of apple and blackcurrant musts and wines in order to evaluate the changes occurring in a simulated human digestive system. The research material consisted of apples and blackcurrant, from which musts and fruit wines were obtained. All samples were subjected to three-stage digestion in a simulated human digestive system and then analyzed for the following: TPC (Folin–Ciocalteu method) and profile (HPLC), AOX (method with 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic) acid (ABTS) radical), and for the wines also total extract, volatile acidity (International Organization of Vine and Wine (OIV) method), and sugar profile (HPLC). The antioxidant activity of fruit wines is directly related to the total polyphenol content. Phenolic compounds were transformed during all digestive stages, which led to the formation of compounds with higher antioxidant capacity. The largest increase in polyphenols was observed after the digestive stage in the small intestine. Transformations of phenolic compounds at each digestive stage resulted in the formation of derivatives with higher antioxidant potential.


2016 ◽  
Vol 4 ◽  
pp. 17-24 ◽  
Author(s):  
Valeriya Ananieva ◽  
Lidiya Krichkovska ◽  
Anna Belinska ◽  
Volodimir Dubonosov ◽  
Sergiy Petrov

It is suggested to perfect technology of production of emulsive foodstuffs, as most consumed by all groups of population of Ukraine, by introduction to compounding of secondary wastes of exit-juice production and vine making as dry concentrates. Such method of enriching of foodstuffs will allow to bring down the deficit of necessary micro- and macronutrients, biologically active substances, and also to bring down indexes on the number of peroxide in the process of storage of low-caloric sauces due to being of natural antioxidants in powdered plant and vegetable material. Experiments were conducted for determination of micro- and macroelement composition and content of sum of polyphenolic substances samples of powdered plant and vegetable material. Samples with the optimal above-mentioned indexes were revealed and samples for the next stage of researches were chosen. The data plan experiment on the effect of temperature and time storage of powdered vegetable raw materials in the amount polyphenols (% wt) in samples of powdered vegetable raw materials used in the suggested corrections storage term low-calorie sauces based on secondary products of juice and wine manufacturing in recipes and calculations in compounding on productions.It was substantiated the choice of grape skin powder as bioactive addition in the emulsive foodstuffs for health improvment purposes, as a sample that has optimal indexes for micro- and macronutrients composition and maintenance of natural antioxidants of polyphenolic nature.


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