scholarly journals Chemical Composition and Antimicrobial Activity of the Essential Oils of Three Closely Related Hypericum Species Growing Wild on the Island of Crete, Greece

2020 ◽  
Vol 10 (8) ◽  
pp. 2823
Author(s):  
Maria-Eleni Grafakou ◽  
Aggeliki Diamanti ◽  
Eleftheria Antaloudaki ◽  
Zacharias Kypriotakis ◽  
Ana Ćirić ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Antibacterial Activities ◽  
Retention Indices ◽  
Qualitative And Quantitative ◽  
Germacrene D ◽  
Chemotaxonomic Markers ◽  
The Given ◽  
Hypericum Species ◽  
Type Apparatus

The volatile compositions of three closely related Hypericum species growing wild on the island of Crete were studied, all belonging to the section Coridium. Hydro-distillation in a modified Clevenger-type apparatus was performed according to the Hellenic Pharmacopoeia in order to obtain the essential oils, which were analyzed by GC-MS. Identification of the compounds was carried out by comparison of MS spectra and retention indices with literature data, as well as by co-chromatography with authentic samples. In total, 123 different compounds were identified and the main compounds were by order of their abundance as follows: H. empetrifolium: α-pinene, germacrene D, β-pinene, E-caryophyllene; H. amblycalyx: β-elemene, β-selinene, α-pinene, E-caryophyllene, α-selinene; H. jovis: trans-calamenene, α-selinene, β-elemene. The chemical results revealed the differences and similarities (qualitative and quantitative) between the studied oils, supporting the hypothesis that essential oils from Hypericum spp. do not serve as chemotaxonomic markers. Moreover, the essential oils were subjected to antimicrobial screening. According to the given results, the essential oils possessed better antifungal and anticandidal activities than antibacterial activities.

scholarly journals Volatile Constituents of Two Hypericum Species from Tunisia

2011 ◽  
Vol 6 (11) ◽  
pp. 1934578X1100601 ◽  
Author(s):  
Karim Hosni ◽  
Kamel Msaâda ◽  
Mouna Ben taârit ◽  
Thouraya Chahed ◽  
Brahim Marzouk
Keyword(s):  
Chemical Composition ◽  
Genetic Variability ◽  
Essential Oils ◽  
Hypericum Perforatum ◽  
Qualitative And Quantitative ◽  
Aerial Parts ◽  
Branched Hydrocarbons ◽  
Chemical Variability ◽  
Germacrene D ◽  
Hypericum Species

The chemical composition of the essential oils obtained by hydrodistillation from the aerial parts of the Tunisian Hypericum perforatum and H. ericoides ssp. roberti was elucidated by a combination of GC and GC-MS analyses. The main constituents of the oil of H. perforatum were α-pinene (11.8%), α-ylangene (10.4%), germacrene-D (9.5%), n-octane (6.5%) and α-selinene (5.9%). The oil of H. ericoides ssp. roberti exhibited a higher amount of aliphatic and branched hydrocarbons and the main constituents were n-octane (29.1%), α-pinene (10.9%), pulegone (7.7%) and acetophenone (7%). Both qualitative and quantitative differences were observed between the studied oils. This chemical variability seems likely to result from the genetic variability, since samples of both species were collected at the same location and processed under the same conditions.

scholarly journals GC/MS Analysis and Antimicrobial Activity of the Essential Oil of Fresh Leaves of Eucalytus Globulus, and Leaves and Stems of Smyrnium Olusatrum from Constantine (Algeria)

2010 ◽  
Vol 5 (10) ◽  
pp. 1934578X1000501 ◽  
Author(s):  
Habiba Daroui-Mokaddem ◽  
Ahmed Kabouche ◽  
Mabrouka Bouacha ◽  
Boudjemaa Soumati ◽  
Aida El-Azzouny ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Eucalyptus Globulus ◽  
Bacterial Strains ◽  
Ms Analysis ◽  
Wide Range ◽  
Main Components ◽  
Type Apparatus

The essential oils of fresh leaves of Eucalyptus globulus L. (Myrtaceae), and leaves and stems of Smyrnium olusatrum L. (Apiaceae), obtained by hydrodistillation in a Clevenger-type apparatus, were analyzed by GC/MS. Twenty compounds were characterized, representing 98.3% of the essential oil of E. globulus, with 1,8-cineole (48.6%), globulol (10.9%), trans-pinocarveol (10.7%), and α-terpineol (6.6%) as main components, while thirty-three compounds were characterized, representing 94.3% of the essential oil of S. olusatrum, with sabinene (27.1%), curzerene (13.7%), methyl-1-benzyl-2-oxocyclooctane carboxylate (12.3%), α-pinene (7.2%), cryptone (7.1%) and β-pinene (5.7%) as the major components. The essential oils of E. globulus and S. olusatrum were tested against a wide range of fungal and bacterial strains. Both oils showed significant antimicrobial activity.

scholarly journals Antibacterial Activities of Essential Oils Extracted from Leaves of Murraya koenigii by Solvent-Free Microwave Extraction and Hydro-Distillation

2012 ◽  
Vol 7 (1) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Naciye Erkan ◽  
Zhou Tao ◽  
H. P. Vasantha Rupasinghe ◽  
Burcu Uysal ◽  
Birsen S. Oksal
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Radical Scavenging ◽  
Antibacterial Activities ◽  
Solvent Free ◽  
Microwave Extraction ◽  
Murraya Koenigii ◽  
Inhibition Of Growth ◽  
Germacrene D ◽  
Dpph Radical Scavenging

Solvent-free microwave extraction (SFME) for the isolation of essential oil from leaves of Murraya koenigii L. (Rutaceae) has been compared with the conventional hydro-distilled oil (HD) in terms of yield, composition, antioxidant activity, and antibacterial activity against Listeria innocua. The yield of essential oil obtained from 30 min of SFME was similar to that of HD for 180 min. By GC-MS analysis, the major compounds of the essential oil extracted by SFME, which were obtained in somewhat lower amounts than in the essential oil obtained by HD, were α-copaene (44.3%), β-gurjunene (25.5%), isocaryophyllene (12.1%), β-caryophyllene (8.7%) and germacrene D (2.9%). The content of oxygenated terpenes, slightly higher for the SFME-essential oil (2.3%) than the HD-essential oil (1.4%), were much lower than that of nonoxygenated terpenes in both oils. DPPH radical scavenging activities of both essential oils were relatively low (10%-24%). Complete inhibition of growth of L. innocua was observed with both SFME and HD essential oils, at 400 and 600 μg/mL (minimum inhibitory concentration), respectively. The SFME-essential oil at 300 μg/mL provided 92% inhibition, indicating its potential as a natural antimicrobial agent.

scholarly journals Composition of the Essential Oils and Antibacterial Activities of Hymenocrater yazdianus, Stachys obtusicrena and Nepeta asterotricha Three Labiatae Herbs Growing Wild in Iran

2012 ◽  
Vol 7 (1) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Shiva Masoudi ◽  
Abdolhossein Rustaiyan ◽  
Razieh Mohebat ◽  
Mohammad Hossein Mosslemin
Keyword(s):  
Essential Oils ◽  
Antibacterial Activities ◽  
Gram Negative Bacteria ◽  
Inhibitory Zone ◽  
Growth Inhibitory ◽  
Germacrene D ◽  
Leaf Oil ◽  
Main Components ◽  
Total Oil ◽  
Stem Leaf

Water-distilled essential oils from leaves of Hymenocrater yazdianus Rech.f., flowers of Stachys obtusicrena Boiss., and stems and flowers of Nepeta asterotricha Rech.f, which are endemic to Iran, were analyzed by GC and GC/MS. Fifty-five components of the leaf oil of H. yazdianus were characterized, representing 95.1% of the total components detected. The major constituents were identified as 1,8-cineole (17.6%), β-caryophyllene (13.9%), α-pinene (10.6%) and caryophyllene oxide (10.4%). Germacrene-D (37.5%) and α-bisabolol (23.5%) were the main components among the twenty constituents characterized in the flower oil of S. obtusicrena, representing 90.8% of the total components detected. Thirty-five compounds representing 93.0% of the stem oil of N. asterotrica were identified among which terpinen-4-ol (22.8%) and γ-terpinene (14.1%) were the major ones. The flower oil of the species was characterized by higher amounts of terpinen-4-ol (24.8%), 4aα, 7aβ-nepetalactone (18.2%) and 1,8-cineole (11.6%) among the thirty-three components comprising 98.5% of the total oil detected. The antibacterial activity of the stem, leaf and flower oils of Hymenocrater yazdianus, Stachys obtusicrena and Nepeta asterotricha against seven Gram-positive and Gram-negative bacteria were determined using the MIC method. The growth inhibitory zone (mm) was also measured.

scholarly journals Essential Oil from the Leaves of Annona vepretorum: Chemical Composition and Bioactivity

2012 ◽  
Vol 7 (2) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Emmanoel Vilaça Costa ◽  
Lívia Macedo Dutra ◽  
Paulo Cesar de Lima Nogueira ◽  
Valéria Regina de Souza Moraes ◽  
Marcos José Salvador ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Trypanosoma Cruzi ◽  
Candida Tropicalis ◽  
Trypanocidal Activity ◽  
Germacrene D ◽  
Weak Activity ◽  
Mic Value ◽  
Type Apparatus

The essential oil from the leaves of Annona vepretorun was obtained by hydrodistillation using a Clevenger-type apparatus and analyzed by GC-MS and GC-FID. Eighteen compounds representing 98.1% of the crude essential oil were identified. The major compounds identified were bicyclogermacrene (43.7%), spathulenol (11.4%), α-felandrene (10.0%), α-pinene (7.1%), (E)-β-ocimene (6.8%), germacrene D (5.8%), and p-cymene (4.2%). The trypanocidal activity against Trypanosoma cruzi epimastigote forms, as well as, the antimicrobial and antioxidant proprieties was investigated. The essential oil showed a potent trypanocidal activity with IC50 value of 31.9±1.3μg.mL-1. For antimicrobial activity, the best result was observed against Candida tropicalis with a MIC value of 100μg.mL-1. For antioxidant capacity the essential oil showed weak activity.

scholarly journals Chemical composition and antimicrobial activity of Myrtus communis essential oils from Algeria

2021 ◽  
Vol 22 (2) ◽  
Author(s):  
YACINE MOHAMADI ◽  
TAKIA LOGRADA ◽  
Messaoud Ramdani ◽  
GILLES FIGUEREDO ◽  
PIERRE CHALARD
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Bacterial Species ◽  
Antibacterial Activities ◽  
Geranyl Acetate ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Myrtus Communis ◽  
Disk Diffusion Method

Abstract. Mohamadi Y, Lograda T, Ramdani M, Figueredo G, Chalard P. 2021. Chemical composition and antimicrobial activity of Myrtus communis essential oils from Algeria. Biodiversitas 22: 933-946.  The objectives of this study were to determine the chemical composition and the antibacterial activities of Myrtus communis essential oils. The aerial parts of the myrtle were collected from nineteen localities of the Algerian coast, and subjected to hydrodistillation. The chemical composition of essential oils was analyzed using gas chromatography coupled with mass spectrometry (GC/MS). The antibacterial activity was evaluated by the disk diffusion method against ten bacterial species. Essential oils of M. communis was pale yellow oils, very fragrant, with an average yield of 0.68 ± 0.6%. Forty-six compounds were identified, representing an average of 99.17 ± 0.6% of total oils. The α-pinene was the most abundant component in the essential oils of all populations studied, with a mean of 50.1 ± 10.3%, followed by 1,8-cineole (22.27 ± 4.32%), limonene (5.16 ± 2.67%), linalool (3.79 ± 2.97%), α-terpineol (2.75 ± 1.12%), geranyl acetate (1.95 ± 0.7%) and methyl eugenol (1.40 ± 0.98%). Myrtenyl acetate was only observed in four populations with significant levels (from 10.66 to 20.94%). Six chemotypes have been identified in these oils. The α-pinene – 1,8-cineole - limonene chemotype was found in the majority of populations. The chemotype of α-pinene - 1,8-cineole - α-terphenyl acetate characterizes the Oued El-Aneb population. The α-pinene - neryl formate - limonene chemotype is observed in the Honaïn population. The remaining populations are characterized by myrtenyl acetate. The essential oils of M. communis were effective against Staphylococcus aureus ATCC 25923, Salmonella enterica ATCC35659, Proteus mirabilis, and Escherichia coli ATCC 25922; however, no significant effect was observed on Klebsiella pneumonia ATCC 700603 and Serratia liquefaciens.

scholarly journals Chemical Composition and Anti-mildew Activities of Essential Oils from Different Parts of Michelia compressa var. formosana

2015 ◽  
Vol 10 (4) ◽  
pp. 1934578X1501000
Author(s):  
Yu-Chang Su ◽  
Kuang-Ping Hsu ◽  
Eugene I-Chen Wang ◽  
Chen-Lung Ho
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Main Constituent ◽  
Germacrene D ◽  
Leaf Oil ◽  
Essential Oil Yield ◽  
Different Parts ◽  
Type Apparatus

In this study, anti-mildew activities of the essential oils of the leaves, wood, twigs, flowers, and their constituents from Michelia compressa var. formosana were evaluated in vitro against 7 mildew fungi. The main compounds responsible for the anti-mildew activities were isolated and identified. The essential oils from the fresh leaves, wood, twigs, and flowers of M. compressa var. formosana were isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS. The essential oil yield from the flowers was the highest, and the oils obtained from the 4 parts of the tree were composed mainly of sesquiterpenoids. The leaf oil consisted primarily of α-cadinol (18.9%) and germacrene D (18.5%); the twig oil's main constituent was α-cadinol (19.1%); the wood oil's major components were α-cadinol (25.7%) and β-eudesmol (20.2%); and the flower oil contained α-cadinol (11.4%). Comparing the anti-mildew activities of the oils suggested that wood oil was the most effective. Further fractionation of the wood oil produced α-cadinol, β-eudesmol, τ-cadinol, and elemol. The 4 compounds exhibit very strong anti-mildew activities, and these in descending order are α-cadinol, elemol, β-eudesmol, and τ-cadinol. For the anti-mildew activities of the wood oil, the active source compounds were determined to be α-cadinol, elemol, β-eudesmol, and τ-cadinol.

scholarly journals Cedrus libani ve Pinus nigra subsp. pallasiana’nın Uçucu Yağlarının Kimyasal Bileşimi, Antimikrobiyal Aktivitesi ve Flavonoid İçeriklerinin Belirlenmesi

2020 ◽  
Vol 8 (8) ◽  
pp. 1747-1754
Author(s):  
Ayşe Nur Demirci ◽  
Nazan Çömlekçioğlu ◽  
Ashabil Aygan
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Pinus Nigra ◽  
Essential Oil Composition ◽  
Diffusion Method ◽  
Oil Composition ◽  
Disc Diffusion Method ◽  
Cedrus Libani ◽  
Germacrene D

Essential oil composition, antimicrobial activity and flavonoid contents of leaf-fruits of Cedrus libani and Pinus nigra subsp. pallasiana were determined with GC-MS, disc diffusion method and HPLC in three different period. When the essential oil composition of leaf and fruits of P. nigra ssp pallasiana collected in April, July and September, major components were α-pinene, β-pinene, limonene, β-caryophyllene ve germacrene-D. On the other hand, additionally to these contents, myrcene and α–terpineol were also detected in C. libani. According to HPLC analysis, rutin, quercetin, kaempferol, naringin and resveratrol flavonoids were detected in different proportions. While rutin (154.33 µg g-) and resveratrol (20.02 µg g-) has the highest ratio in C. libani, quercetin (9.65 µg g-) and naringin (9.31 µg g-) were detected in P. nigra subsp. pallasiana along with rutin (39.66 µg g-). According to the antimicrobial activity results the essential oils of C. libani obtained in April has produced higher activity than that of July and September. On the contrary, the essential oils from P. nigra subsp. pallasina have produced the best antimicrobial activity on September compared to April and July. As a result, C. libani and P. nigra subsp. pallasiana essential oils have a composition showing antimicrobial activity and their harvesting season should be determined for the best and effective content.

scholarly journals Antimicrobial activity of berries and leaves essential oils of Macedonian Juniperus foetidissima Willd. (Cupressaceae)

2015 ◽  
Vol 61 (01) ◽  
pp. 3-11 ◽  
Author(s):  
Floresha Sela ◽  
Marija Karapandzova ◽  
Gjose Stefkov ◽  
Ivana Cvetkovikj ◽  
Elena Trajkovska-Dokikj ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Essential Oil ◽  
Essential Oils ◽  
Dilution Method ◽  
Moderate Activity ◽  
Gram Negative Bacteria ◽  
Bacterial Strains ◽  
Germacrene D ◽  
Broth Dilution

Chemical composition and antimicrobial activity of leaves and berries essential oils from Juniperus foetidissima Willd. (Cupressaceae) grown in R. Macedonia (RM) was investigated. GC/FID/MS analysis was carried out and 93 components were identified, representing 89.7-96.5% of the oils. The major components of the berries essential oil were α-pinene (19.2%), limonene (24.9%) and cedrol (23.1%), followed by smaller amounts of b-funebrene, trans-caryophyllene, germacrene D and d-cadinene. The composition of the leaves essential oil was variable depending on the region of collection. Accordingly, samples originated from southeastern RM contained essential oil with α-pinene (67.6%) and limonene (10.0%), from central part of RM with limonene (17.9-27.1%) and cedrol (28.8-33.9%), while samples from southwestern RM contained oil with terpinen-4-ol (19.1%), cis-thujone (8.3%), germacrene D (11.0%) and d-cadinene (6.3%) as predominant components in the oil. Antimicrobial screening of the essential oils was made by disc diffusion and broth dilution method against 16 bacterial strains of Gram-positive and Gram-negative bacteria and one strain of Candida albicans. The leaves essential oil showed stronger antimicrobial activity against Staphylococcus aureus, Streptococcus pyogenes and Haemophilus influenzae (MIC = 125 ml/ml) and moderate activity against Campylobacter jejuni (MIC > 500 ml/ml). Other investigated bacterial strains and Candida albicans were completely resistant to the antimicrobial activity of J. foetidissima essential oils.

scholarly journals Chemical Composition and Anti-Trypanosoma cruzi Activity of Essential Oils Obtained from Leaves of Xylopia frutescens and X. laevigata (Annonaceae)

2013 ◽  
Vol 8 (3) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Thanany Brasil da Silva ◽  
Leociley Rocha Alencar Menezes ◽  
Marília Fernanda Chaves Sampaio ◽  
Cássio Santana Meira ◽  
Elisalva Teixeira Guimarães ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Trypanosoma Cruzi ◽  
Essential Oils ◽  
Trypanocidal Activity ◽  
Germacrene D ◽  
Main Components ◽  
Type Apparatus

Essential oils from leaves of Xylopia frutescens (XFMJ) and two specimens of Xylopia laevigata (XLMC and XLSI) were obtained by hydrodistillation using a Clevenger-type apparatus, and analyzed by GC-MS and GC-FID. Sesquiterpenes dominated the essential oils. The main constituents of XFMJ were ( E)-caryophyllene (24.8%), bicyclogermacrene (20.8%), germacrene D (17.0%), β-elemene (7.9%), and ( E)-β-ocimene (6.8%). XLMC contained significant quantities of germacrene D (18.9%), bicyclogermacrene (18.4%), β-elemene (9.5%), 5-selinene (9.2%), ( E)-caryophyllene (8.5%), germacrene B (5.7%) and γ-muurolene (5.7%), while germacrene D (27.0%), bicyclogermacrene (12.8%), ( E)-caryophyllene (8.6%), γ-muurolene (8.6%), 5-cadinene (6.8%), and germacrene B (6.0%) were the main components of XLSI. The essential oils had trypanocidal activity against the Y strain of Trypanosoma cruzi. with IC50 values lower than 30 μg.mL−1 and 15 μg.mL−1 against epimastigote and trypomastigote forms of T. cruzi. respectively, and were also able to reduce the percentage in vitro of T. cruzi-infected macrophages and the intracellular number of amastigotes at concentrations that were non-cytotoxic to macrophages.

Sign in / Sign up

Export Citation Format

Share Document