scholarly journals An Anthocyanin-Enriched Extract from Vaccinium uliginosum Improves Signs of Skin Aging in UVB-Induced Photodamage

Antioxidants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 844
Author(s):  
Kyungae Jo ◽  
Gi Yeon Bae ◽  
Kyoungwon Cho ◽  
Sung Sun Park ◽  
Hyung Joo Suh ◽  
...  
Keyword(s):  
Oral Administration ◽  
High Performance ◽  
Molecular Mechanisms ◽  
Transepidermal Water Loss ◽  
Ultraviolet B ◽  
Parameter Analysis ◽  
Tissue Inhibitor Of Metalloproteinase ◽  
Biophysical Parameters ◽  
Hairless Mice ◽  
Vaccinium Uliginosum

Accumulating evidence indicates that botanical extracts affect skin biophysical parameters, such as hydration, transepidermal water loss (TEWL), melanin index, erythema index, and wrinkle development. Vaccinium uliginosum extract contains a high level of anthocyanins as antioxidant and is ideal for use in dietary skin care products. Here, we assessed the photoprotective effects of dietary V. uliginosum extract in ultraviolet B (UVB)-irradiated hairless mice. Quantitative analysis of anthocyanin composition in the ethanol-extracted V. uliginosum sample was performed using high-performance liquid chromatography (HPLC). Skin parameter analysis and hematoxylin and eosin (H&E) staining were conducted on skin samples from UVB-irradiated hairless mice to evaluate the effects of V. uliginosum extract on skin conditions. In addition, skin mRNA and protein expression were assessed to characterize the molecular mechanisms underlying the effects of the anthocyanin-enriched extract on skin appearance and condition. Administration of the ethanol-extracted V. uliginosum sample caused significant changes in skin water-holding capacity, TEWL, wrinkle-related parameters, and epidermal thickness in UVB-irradiated hairless mice. In addition, oral administration of V. uliginosum attenuated the gene expression of matrix metalloproteinase (MMP) and increased levels of tissue inhibitor of metalloproteinase (TIMP) and antioxidant-related genes. Further, V. uliginosum administration downregulated inflammatory cytokine levels and UVB-induced phosphorylation of extracellular signaling regulated kinase (ERK), as well as Jun N-terminal kinase (JNK) and p38 protein levels. Oral administration of anthocyanin-enriched V. uliginosum extract can improve the appearance and condition of the skin following UV irradiation.

scholarly journals Oral Intake of Collagen Peptide Attenuates Ultraviolet B Irradiation-Induced Skin Dehydration In Vivo by Regulating Hyaluronic Acid Synthesis

2018 ◽  
Vol 19 (11) ◽  
pp. 3551 ◽  
Author(s):  
Min Kang ◽  
Silvia Yumnam ◽  
Sun Kim
Keyword(s):  
Hyaluronic Acid ◽  
Oral Administration ◽  
Protein Expression ◽  
Ultraviolet B ◽  
Skin Hydration ◽  
Skin Barrier Function ◽  
Hairless Mice ◽  
Uvb Irradiation ◽  
Collagen Peptide

Collagen peptide (CP) has beneficial effects on functions of the skin, such as skin barrier function and skin elasticity, in vivo. However, there are few studies investigating the mechanism underlying the potential effects of CP in skin epidermal moisturization after ultraviolet B (UVB) irradiation. In this study, we examined whether orally-administered CP affects the loss of skin hydration induced by UVB irradiation in hairless mice. SKH-1 hairless mice were orally administered CP at two doses (500 and 1000 mg/kg) for nine weeks, and the dorsal skin was exposed to UVB. The potential effects of CP were evaluated by measuring the transepidermal water loss (TEWL), skin hydration, wrinkle formation, and hyaluronic acid expression in the dorsal mice skin. We found that oral administration of CP increased skin hydration and decreased wrinkle formation compared to the UVB-irradiated group. Treatment of CP increased the mRNA and protein expression of hyaluronic acid synthases (HAS-1 and -2) concomitant with an increased hyaluronic acid production in skin tissue. The expression of hyaluronidase (HYAL-1 and 2) mRNA was downregulated in the CP-treated group. In addition, the protein expression of skin-hydrating factors, filaggrin and involucrin, was upregulated via oral administration of CP. In summary, these results show that oral administration of CP increases hyaluronic acid levels, which decreases during UVB photoaging. Therefore, we suggest that CP can be used as a nutricosmetic ingredient with potential effects on UVB-induced skin dehydration and moisture loss in addition to wrinkle formation.

scholarly journals Oral administration of bovine lactoferrin attenuates ultraviolet B-induced skin photodamage in hairless mice

2014 ◽  
Vol 97 (2) ◽  
pp. 651-658 ◽  
Author(s):  
M. Murata ◽  
T. Satoh ◽  
H. Wakabayashi ◽  
K. Yamauchi ◽  
F. Abe ◽  
...  
Keyword(s):  
Oral Administration ◽  
Ultraviolet B ◽  
Bovine Lactoferrin ◽  
Hairless Mice

scholarly journals Oral administration of Jumihaidokuto inhibits UVB-induced skin damage and prostaglandin E2 production in HR-1 hairless mice

2020 ◽  
Vol 75 (1) ◽  
pp. 142-155
Author(s):  
Kenta Murata ◽  
Manami Oyama ◽  
Misaki Ogata ◽  
Nina Fujita ◽  
Ryuji Takahashi
Keyword(s):  
Oral Administration ◽  
Prostaglandin E2 ◽  
Water Content ◽  
Transepidermal Water Loss ◽  
Skin Disorders ◽  
Skin Damage ◽  
Hairless Mice ◽  
Traditional Use ◽  
Uvb Irradiation ◽  
Proinflammatory Mediators

AbstractThis study was conducted to investigate whether and how Jumihaidokuto (JHT), a traditional Chinese medicine, prevents UVB-induced skin damage in male HR-1 hairless mice. JHT has been traditionally prescribed for patients presenting skin disorders with redness and swelling, and, in Japan, it is approved for prescription to patients with acute and/or purulent skin disorders, hives, acute eczema, and athlete’s foot. Considering the traditional use of JHT, we hypothesized that oral administration of JHT might emerge as an effective strategy to prevent UVB-induced skin damage, such as edema and erythema. Here, we pretreated mice with JHT (1000 mg/kg, p.o.) for 3 weeks and then administered a single dose of UVB irradiation (250 mJ/cm2) on the dorsal skin. UVB irradiation increased the erythema index and transepidermal water loss (TEWL) and decreased the skin water content in the epidermis at 72 h post-irradiation. JHT treatment inhibited the increase of TEWL and the loss of water content in the epidermis, but not the elevation of the erythema index. Moreover, administration of JHT suppressed UVB-induced epidermal hyperplasia by blocking the proliferation of keratinocytes and also inhibited irradiation-triggered reduction of collagen fibers and infiltration of immune cells into the dermis. Lastly, administration of JHT suppressed UVB-induced production of proinflammatory mediators, such as prostaglandin E2 and interleukin-1β. These results suggest that JHT prevents UVB-induced skin damage and that the underlying mechanism involves the inhibition of proinflammatory mediators.

scholarly journals Biophysical parameters of rats’ skin after the administration of methimazole

2014 ◽  
Vol 58 (2) ◽  
pp. 315-319 ◽  
Author(s):  
Marcin Gołyński ◽  
Marcin Szczepanik ◽  
Krzysztof Lutnicki ◽  
Łukasz Adamek ◽  
Magdalena Gołyńska ◽  
...  
Keyword(s):  
Oral Administration ◽  
Water Loss ◽  
Wistar Rats ◽  
Skin Barrier ◽  
Transepidermal Water Loss ◽  
Skin Hydration ◽  
Barrier Dysfunction ◽  
Early Assessment ◽  
Biophysical Parameters ◽  
Ph Measurements

Abstract The paper describes the influence of oral administration of methimazole on biophysical skin parameters. Wistar rats of different sex (220-260 g) were used in the experiment. Biophysical skin parameters, such as transepidermal water loss (TEWL), corneometry, and pH were examined at seven-day intervals. Significant changes in the parameters were observed on the 7th d of methimazole administration. The changes were observed in both sex but males appeared to be less sensitive in that respect. Changes in the parameters in the females showed rapid mechanisms, which normalised transepidermal water loss and skin hydration, as well as restored skin barrier functions. TEWL, skin hydration, and skin pH measurements allow an early assessment of skin barrier dysfunction after administration of this drug.

scholarly journals Echinochrome A Protects against Ultraviolet B-induced Photoaging by Lowering Collagen Degradation and Inflammatory Cell Infiltration in Hairless Mice

Marine Drugs ◽  
2021 ◽  
Vol 19 (10) ◽  
pp. 550
Author(s):  
Jung Eun Seol ◽  
Sang Woo Ahn ◽  
Bomin Seol ◽  
Hyeong Rok Yun ◽  
Nammi Park ◽  
...  
Keyword(s):  
Water Loss ◽  
Inflammatory Cell ◽  
Transepidermal Water Loss ◽  
Collagen Degradation ◽  
Ultraviolet B ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration ◽  
Skin Barrier Function ◽  
Hairless Mice ◽  
Echinochrome A

Echinochrome A (Ech A, 7-ethyl-2,3,5,6,8-pentahydroxy-1,4-naphthoquinone) has been known to exhibit anti-oxidative and anti-inflammatory effects. However, no study has been carried out on the efficacy of Ech A against skin photoaging; this process is largely mediated by oxidative stress. Six-week-old male SKH-1 hairless mice (n = 36) were divided into five groups. Except for a group that were not treated (n = 4), all mice underwent ultraviolet-B (UVB) exposure for 8 weeks while applying phosphate-buffered saline or Ech A through intraperitoneal injection. UVB impaired skin barrier function, showing increased transepidermal water loss and decreased stratum corneum hydration. UVB induced dermal collagen degeneration and mast cell infiltration. Ech A injection was found to significantly lower transepidermal water loss while attenuating tissue inflammatory changes and collagen degeneration compared to the control. Furthermore, Ech A was found to decrease the relative expression of matrix metalloproteinase, tryptase, and chymase. Taken together, these results suggest that Ech A protects against UVB-induced photoaging in both functional and histologic aspects, causing a lowering of collagen degradation and inflammatory cell infiltration.

Effect of Oral Administration of Lactobacillus plantarum HY7714 on Epidermal Hydration in Ultraviolet B-Irradiated Hairless Mice

2014 ◽  
Vol 24 (12) ◽  
pp. 1736-1743 ◽  
Author(s):  
Jehyeon Ra ◽  
Dong Eun Lee ◽  
Sung Hwan Kim ◽  
Ji-Woong Jeong ◽  
Hyung Keun Ku ◽  
...  
Keyword(s):  
Oral Administration ◽  
Lactobacillus Plantarum ◽  
Ultraviolet B ◽  
Hairless Mice

scholarly journals Protective Effects of Kuding Tea (Ilex kudingcha C. J. Tseng) Polyphenols on UVB-Induced Skin Aging in SKH1 Hairless Mice

Molecules ◽  
2019 ◽  
Vol 24 (6) ◽  
pp. 1016 ◽  
Author(s):  
Ruokun Yi ◽  
Jing Zhang ◽  
Peng Sun ◽  
Yu Qian ◽  
Xin Zhao
Keyword(s):  
Uv Light ◽  
Skin Aging ◽  
Ultraviolet B ◽  
Tissue Inhibitor Of Metalloproteinase ◽  
Matrix Metalloproteinase 2 ◽  
Precipitation Method ◽  
Protective Effects ◽  
Skin Damage ◽  
Hairless Mice ◽  
Necrosis Factor Alpha

In this study, the protective effects of Kuding tea polyphenols (KTPs) on ultraviolet B (UVB)-induced skin injury of SKH1 hairless mice were studied. The ion precipitation method was used for extraction of polyphenols from Kuding tea. High-performance liquid chromatography showed that KTPs contains chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B, isochlorogenic acid A, and isochlorogenic acid C. SKH1 hairless mice were induced skin aging using 2.0 mW/s intensity of 90 mJ/cm2 UV light once a day for seven weeks. The 2.5% and 5% KTPs solution was smeared on 2 cm2 of back skin of skin aging mice twice a day. Mouse experiments showed that KTP strongly increased the serum levels of total superoxide dismutase (T-SOD) and catalase (CAT) and reduced those of malondialdehyde, interleukin 6 (IL-6), IL-1β, and tumor necrosis factor alpha (TNF-α) in mice with UVB-induced skin damage. KTP also increased the levels of type 1 collagen (Col I), hydroxyproline, and hyaluronic acid and reduced those of Col III and hydrogen peroxide in the damaged skin tissues of mice. Pathological observations of tissues stained with H & E, Masson’s trichrome, Verhoeff, and toluidine blue showed that KTPs could protect skin cells, collagen, and elastin and decrease the number of mast cells, thus inhibiting skin damage. Quantitative PCR and western blot assays showed that KTP upregulated the mRNA and protein expression of tissue inhibitor of metalloproteinase 1 (TIMP-1), TIMP-2, copper/zinc-SOD, manganese-SOD, CAT, and glutathione peroxidase and downregulated the expression of matrix metalloproteinase 2 (MMP-2) and MMP-9. In addition, the same concentration of KTP had stronger protective effects than vitamin C. The results of this study demonstrate that KTPs have good skin protective effects, as they are able to inhibit UVB-induced skin damage.

The examination of biophysical parameters of skin (transepidermal water loss, skin hydration and pH value) in different body regions of ponies

2012 ◽  
Vol 15 (3) ◽  
pp. 553-559 ◽  
Author(s):  
M.P. Szczepanik ◽  
P.M. Wilkołek ◽  
M. Pluta ◽  
Ł.R. Adamek ◽  
Z.J.H. Pomorski
Keyword(s):  
Water Loss ◽  
Ph Value ◽  
Neck Region ◽  
Transepidermal Water Loss ◽  
Skin Hydration ◽  
Lumbar Region ◽  
Biophysical Parameters ◽  
Body Regions ◽  
Skin Ph

Abstract The purpose of this study was to evaluate transepidermal water loss, skin hydration and skin pH in normal ponies. Sixteen ponies of both sexes were examined in the study. Measurements were taken from seven different sites: the neck region, the shoulder, thorax, lumbar, inguinal, lip region and the auricle. In each of the regions transepidermal water loss (TEWL), skin hydration and skin pH were measured. For transepidermal water loss, the lowest values were observed in the lumbar region (9.71g/hm2), while the highest values were observed in the lip region (22.35 g/hm2). In the case of skin hydration the lowest values were observed for the thorax region (2.13 CU), and the highest for the lip region (41.81 CU). For skin pH, the lowest results were obtained in the lumbar region (6.93), and the highest in the lip region (7.96).

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