scholarly journals Phenylethyl Isothiocyanate Extracted from Watercress By-Products with Aqueous Micellar Systems: Development and Optimisation

Antioxidants ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 698
Author(s):  
Ezequiel R. Coscueta ◽  
Celso A. Reis ◽  
Manuela Pintado
Keyword(s):  
Antioxidant Capacity ◽  
Cost Effective ◽  
Extraction Process ◽  
Antioxidant Compounds ◽  
Chemopreventive Agent ◽  
Micellar Systems ◽  
Stability Issues ◽  
Phenylethyl Isothiocyanate ◽  
First Time ◽  
By Products

Phenylethyl isothiocyanate (PEITC) was reported as a useful antioxidant, anti-inflammatory, and chemopreventive agent. Due to technological and stability issues, it is necessary to be able to extract PEITC from its natural matrix (watercress) through sustainable and scalable methodologies. In this article, we explored, for the first time, the extractive capacity of aqueous micellar systems (AMSs) of two non-ionic surfactants. For this, we compared the AMSs with conventional organic solvents. Furthermore, we developed and optimised a new integral PEITC production and extraction process by a multifactorial experimental design. Finally, we analysed the antioxidant capacity by the oxygen radical absorbance capacity (ORAC) and ABTS methods. As results, the AMSs were able to extract PEITC at the same level as the tested conventional solvents. In addition, we optimised by response surface methodology the integrated process (2.0% m/m, 25.0 °C, pH 9.0), which was equally effective (ca. 2900 µg PEITC/g watercress), regardless of the surfactant used. The optimal extracts showed greater antioxidant capacity than pure PEITC, due to other antioxidant compounds extracted in the process. In conclusion, by the present work, we developed an innovative cost-effective and low environmental impact process for obtaining PEITC extracts from watercress by-products.

scholarly journals Constituents of Chamaecrista diphylla (L.) Greene Leaves with Potent Antioxidant Capacity: A Feature-Based Molecular Network Dereplication Approach

Pharmaceutics ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 681
Author(s):  
Paulo Gomes ◽  
Luis Quirós-Guerrero ◽  
Abraão Muribeca ◽  
José Reis ◽  
Sônia Pamplona ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Ethanolic Extract ◽  
Oxygen Radical ◽  
Molecular Network ◽  
Antioxidant Compounds ◽  
Herbaceous Plant ◽  
Network Approach ◽  
Feature Based ◽  
Promising Source ◽  
First Time

Chamaecrista diphylla (L.) Greene (Fabaceae/Caesalpiniaceae) is a herbaceous plant that is widely distributed throughout the Americas. Plants from this genus have been used in traditional medicine as a laxative, to heal wounds, and to treat ulcers, snake and scorpion bites. In the present study, we investigated the chemical composition of Chamaecrista diphylla leaves through a mass spectrometry molecular network approach. The oxygen radical absorbance capacity (ORAC) for the ethanolic extract, enriched fractions and isolated compounds was assessed. Overall, thirty-five compounds were annotated for the first time in C. diphylla. Thirty-two of them were reported for the first time in the genus. The isolated compounds 9, 12, 24 and 33 showed an excellent antioxidant capacity, superior to the extract and enriched fractions. Bond dissociation energy calculations were performed to explain and sustain the antioxidant capacity found. According to our results, the leaves of C. diphylla represent a promising source of potent antioxidant compounds.

scholarly journals Optimization of High-Pressure Extraction Process of Antioxidant Compounds from Feteasca regala Leaves Using Response Surface Methodology

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4209
Author(s):  
Anca Becze ◽  
Vanda Liliana Babalau-Fuss ◽  
Cerasel Varaticeanu ◽  
Cecilia Roman
Keyword(s):  
Response Surface Methodology ◽  
Antioxidant Capacity ◽  
Response Surface ◽  
Total Antioxidant Capacity ◽  
Raw Materials ◽  
Extraction Process ◽  
Extraction Yield ◽  
Antioxidant Compounds ◽  
Operational Conditions ◽  
Total Antioxidant

Circular economy principles are based on the use of by-products from one operation as the raw materials in another. The aim of this work is to obtain extracts with high antioxidant capacity and resveratrol content for the superior capitalization of the biomass of Feteasca regala leaves obtained during vineyard horticultural operations in spring. In order to obtain a high-quality extract at an industrial level, an optimal extraction process is needed. Central composite design (CCD) was used for the experiment design, which contained three independent variables: the ratio of extraction solvent to solid matter, temperature (°C) and time (minutes). The evaluation of extracts was done by measuring the total antioxidant capacity of the extracts using photo-chemiluminescent techniques, and the resveratrol content using liquid chromatography. Process optimization was done using response surface methodology (RSM). Minitab software version 17.0 was used for the design of experiments and data analysis. Regression analysis showed that the model predicts 87.5% of the variation for resveratrol and 96% for total antioxidant capacity (TAC). The temperature had the biggest influence on the extraction yield. The optimal operational conditions for the extraction method applied had the following conditions: ratio e/m 2.92; 43.23 °C and 55.4 min. A maximum value of 34,623 µg ascorbic acid equivalent (AAE) /mL total antioxidant capacity and 182.4 µg/mL resveratrol content were obtained when the optimal extraction parameters where used. The values obtained in experiments proved that by using RSM an accurate model can be obtained for extraction of Feteasca regala leaves.

scholarly journals Efficiency of extraction of antioxidant compounds in by-product from red guava processing

2017 ◽  
Vol 8 (4) ◽  
pp. 141
Author(s):  
Maira Oliveira Silva ◽  
Gláucia Maria Pastore
Keyword(s):  
Antioxidant Capacity ◽  
Sequential Extraction ◽  
Potential Method ◽  
Antioxidant Compounds ◽  
Single Extraction ◽  
Human Food ◽  
Phenolic Extraction ◽  
By Products

Studies that investigate efficient methods for extraction of antioxidant compounds in by-products of fruits can contribute to their appreciation in human food. This study aimed comparing two methods for phenolic extraction - single extraction (single solvent) and sequential (different solvents) in by-product from Paluma guava processing, evaluating the influence of the extracts on the stabilization of radicals by DPPH and ORAC assays. Sequential extraction showed higher efficiency in obtaining phenolic (2.60 > 0.56 mg GAE g-1), allowing the extraction of free phenolic, conjugated and fat-soluble, as well as determining the   highest antioxidant capacity in these extracts. Acetone was characterized to be the responsible solvent for higher extraction of phenolic in the sequential extraction, being necessary 0.90 mg mL-1 of extract to reduce 50% the action of DPPH• radicals. Thus, sequential extraction of phenolic is a potential method for better use of antioxidant compounds in the guava processing by-product.

scholarly journals Optimisation of the extraction process of antioxidant capacity compounds from beet (Beta vulgaris L.) stalk

2020 ◽  
Vol 41 (6) ◽  
pp. 2621-2632
Author(s):  
Emanueli Backes ◽  
◽  
Aziza Kamal Genena ◽  
Keyword(s):  
Response Surface Methodology ◽  
Free Radicals ◽  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Extraction Process ◽  
Antioxidant Compounds ◽  
Independent Variables ◽  
Potential Source ◽  
Water Ratio ◽  
Reducing Capacity

Beet is a traditional root consumed worldwide and is considered a potential source of several bioactive compounds, yet during production and commercialisation activities, its leaves and stalks are discarded. The beet residue has notable quantities of diverse phenolic compounds that are not taken advantage of it. The beet stalks (sample) were obtained from a local producer at the municipality of Marechal Cândido Rondon, Paraná state, Brazil. The optimisation of the extraction process of antioxidant capacity compounds from beet stalk was done by response surface methodology (RSM), with three independent variables (time, from 5 to 85 min; temperature, from 20 to 80 °C and solvent, from 0 to 100% of ethanol/water ratio). Extracts were evaluated for their reducing capacity, measured by the Folin–Ciocalteu method, and antioxidant capacity by the ability to scavenge DPPH and ABTS free radicals. The optimal global extraction conditions determined were 5 min, 80 °C and 50% ethanol, yielding 13.157 mg gallic acid equivalents (GAE) g–1, 21.539 mol Trolox equivalents g-1 (TE; DPPH) and 250.190 ?mol TE g–1 (ABTS). Beet stalk demonstrated to be an alternative and rich source of recovering of natural antioxidant compounds, showing higher contents when compared to other agro-industrial residues.

scholarly journals Enhancing Bioactive Antioxidants’ Extraction from “Horchata de Chufa” By-Products

Foods ◽  
2018 ◽  
Vol 7 (10) ◽  
pp. 161 ◽  
Author(s):  
Elena Roselló-Soto ◽  
Francisco Barba ◽  
Predrag Putnik ◽  
Danijela Bursać Kovačević ◽  
Jose Lorenzo ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Raw Materials ◽  
Extraction Process ◽  
Total Flavonoids ◽  
Cyperus Esculentus ◽  
Total Polyphenols ◽  
Solvent Type ◽  
Biological Compounds ◽  
Total Antioxidant ◽  
By Products

During the production of a traditional drink produced from the tubers of Cyperus esculentus L. also known as “horchata de chufa,” a high quantity of by-products are generated. These by-products are rich with valuable biological compounds, hence, there is a need to report their extraction conditions for further use in food production as raw materials. Therefore, the objective of this study was to evaluate and improve the conventional extraction process, applied for recovery of phenolic compounds, total flavonoids, and total antioxidant capacity from the by-products. Independent variables for extraction were: (i) Solvent type (mixtures of ethanol-water (v/v) at 0%, 25% and 50%); (ii) temperature (40, 50 and 60 °C), and (iii) extraction time (1, 2 and 3 h). The obtained results showed that solvent type, temperature, and time significantly influenced (p < 0.05) all investigated parameters. The highest content of total polyphenols (16.02 mg GAE/100 g of dry matter; d.m.), and total flavonoids (30.09 mg CE/100 g d.m.) was achieved by ethanol at 25% (v/v), after 3 h of extraction with temperatures of 60 °C and 50 °C, respectively. The highest value of antioxidant capacity (1759.81 µM Trolox equivalents/g d.m.) was observed with 50% aqueous ethanol (v/v), at 60 °C, and 3 h of extraction. From the obtained results, it can be concluded that the by-products of “Horchata de Chufa” are an important source of antioxidant bioactive compounds.

scholarly journals Bioactive Compounds from Leaves and Twigs of Guayule Grown in a Mediterranean Environment

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 442 ◽  
Author(s):  
Giovanna Piluzza ◽  
Giuseppe Campesi ◽  
Maria Giovanna Molinu ◽  
Giovanni Antonio Re ◽  
Leonardo Sulas
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Plant Biomass ◽  
Extraction Process ◽  
Total Phenolic ◽  
Mediterranean Environment ◽  
Methanolic Extracts ◽  
Whole Plant ◽  
Potential Applications ◽  
First Time

Guayule (Parthenium argentatum) is one of the most promising alternatives to produce natural rubber. As the guayule leaves represent a waste byproduct in the rubber extraction process, their exploitation might contribute to the valorization of the whole plant biomass. The specific aim of our study was to determine the antioxidant capacity and the content of phenolic compounds in leaves and twigs of different guayule lines cultivated in a Mediterranean environment. The antioxidant capacity and the contents of phenolic were affected significantly by guayule accession and harvest time. Overall means in twigs represented about 60% of the corresponding leaf values for antioxidant capacity as DPPH (1,1-diphenyl-2-picrylhydrazyl) and not tannic phenolic and about 55% as ABTS (2,2′-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt), total phenolic, tannic phenolic and total flavonoid contents. Five individual phenolic compounds were identified in methanolic extracts of leaves. Neochlorogenic acid (62.5–174.8 mg g−1 DW) and chlorogenic acid (28.6–41.1 mg g−1 DW) were the most abundant phenolic acids. In addition to the compounds quantified in leaves, cynarin was identified only in twigs and for the first time in guayule biomass. Our results indicate that guayule leaves and twigs represent a rich source of antioxidants for potential applications in fodder, nutraceutical and pharmacological sectors.

scholarly journals Valorization of Fig (Ficus carica Linn.) Waste Leaves: HPLC-QTOF-MS/MS-DPPH System For Online Screening And Identification of Antioxidant Compounds

2021 ◽  
Author(s):  
Chunying Li ◽  
Meiting Yu ◽  
Shen Li ◽  
Xue Yang ◽  
Bin Qiao ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Antioxidant Capacity ◽  
Ficus Carica ◽  
Antioxidant Compounds ◽  
Antioxidant Power ◽  
Dpph Method ◽  
Screening And Identification ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
First Time

Abstract Fig (Ficus carica Linn.) leaves are produced each year and often thrown away as wastes, resulting in a waste of resources. Fig waste leaves are rich in flavonoids, which have strong antioxidant activity, however, the antioxidant components are not clear. So as to take full advantage of fig waste leaves, antioxidant capacity of different extracts (petroleum ether, ethyl acetate and water) was evaluated by 1, 1-diphenyl-2-picrylhydrazyl (DPPH), 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic) acid (ABTS) and ferric-ion reducing antioxidant power (FRAP) methods. The results showed that flavonoids in ethyl acetate extraction had the highest content, 83.92 ± 0.01 mg/g, and maximum DPPH scavenging activity (IC50 0.54 mg/mL), highest ABTS scavenging rate (80.28%), FRAP (3.46 mmol/g). Furthermore, HPLC-QTOF-MS/MS-DPPH method was developed for the first time to identify 11 flavonoids in fig waste leaves. This rapid and efficient method can not only be used for screening the antioxidant components in fig waste leaves, but also can be combined with mass spectrometry to identify the compounds with antioxidant capacity. There are three flavonoids with significant antioxidant capacity, which are 3-O-(rhamnopyranosyl-glucopyranosyl)-7-O-(glucopyranosyl)-quercetin, isoschaftoside and rutin. The results confirmed that fig waste leaves contain a variety of antioxidant components, which contributed to increase the value of fig waste leaves as antioxidants.

scholarly journals Aqueous Enzymatic Extraction of Buriti (Mauritia Flexuosa) Oil: Yield and Antioxidant Compounds

2019 ◽  
Vol 11 (1) ◽  
pp. 9-17 ◽  
Author(s):  
Jezica P.P. Silva ◽  
Antonio M.C. Rodrigues ◽  
Luiza H.M. Silva
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Extraction Process ◽  
Aqueous Extraction ◽  
Carotenoid Content ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Total Phenolic Compounds ◽  
Buriti Oil ◽  
Aqueous Extraction Process

Introduction: Enzyme-assisted aqueous extraction is considered an emerging green technique that has been applied to different oilseeds. Objective: This study aimed to study the enzymatic aqueous extraction process of buriti oil using a central composite rotatable design (CCRD) combined with the response surface methodology aiming to obtain higher yield and antioxidant compounds in the oil. Methods: The study was carried out in two steps. The first assessed the efficiency of different enzymes (cellulase, pectinase, and protease) and the variables of greater influence in the extraction process, being conducted for each enzyme a CCRD design. The second step was carried out with the enzyme that showed the best performance on the extraction yield, changing the experimental bands of the variables that had greater significance in the first step, with the goal of broadening the spectrum of study. Were also evaluated in this step, total carotenoids, total phenolic compounds, and the antioxidant capacity of the oils extracted. Results: In the first experiment, cellulase gave the highest yield, while the most significant variables were temperature and time. For the second design, performed with cellulase, were defined as optimal operating conditions at 55 °C temperature, 2% enzyme concentration and 6 hours extraction. For these conditions, the yield obtained was 76.5%, with total carotenoid concentration of 3,119.5 µg β-carotene.g-1. Analysis of variance was performed and showed the significance of the regression and non-significance of the lack-of-fit (p<0.05). The coefficients of determination of the yield and carotenoid content were 95.6% and 94.5%, respectively. The highest value of total phenolic compounds determined for buriti oil in this study was 254 ± 5 µg GAE.g-1 oil, while for the antioxidant capacity was 218.0 ± 0.3 µmol Trolox.g-1 oil. Conclusion: The enzymatic aqueous extraction process is viable for buriti oil and produced oils with high concentrations of antioxidant compounds.

scholarly journals Dried Destoned Virgin Olive Pomace: A Promising New By-Product from Pomace Extraction Process

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4337
Author(s):  
Cinzia Benincasa ◽  
Massimiliano Pellegrino ◽  
Lucia Veltri ◽  
Salvatore Claps ◽  
Carmelo Fallara ◽  
...  
Keyword(s):  
Animal Feed ◽  
Oil Industry ◽  
Extraction Process ◽  
Olive Pomace ◽  
Secondary Products ◽  
Lower Temperature ◽  
Quality Of Milk ◽  
First Time ◽  
By Products

At present the olive oil industry produces large amounts of secondary products once considered waste or by-products. In this paper, we present, for the first time, a new interesting olive by-product named “dried destoned virgin olive pomace” (DDVOP), produced by the pomace oil industry. The production of DDVOP is possible thanks to the use of a new system that differs from the traditional ones by having the dryer set at a lower temperature value, 350 °C instead of 550 °C, and by avoiding the solvent extraction phase. In order to evaluate if DDVOP may be suitable as a new innovative feeding integrator for animal feed, its chemical characteristics were investigated. Results demonstrated that DDVOP is a good source of raw protein and precious fiber; that it is consistent in total phenols (6156 mg/kg); rich in oleic (72.29%), linoleic (8.37%) acids and tocopherols (8.80 mg/kg). A feeding trial was, therefore, carried out on sheep with the scope of investigating the influence of the diet on the quality of milk obtained from sheep fed with DDVOP-enriched feed. The resulting milk was enriched in polyunsaturated (0.21%) and unsaturated (2.42%) fatty acids; and had increased levels of phenols (10.35 mg/kg) and tocopherols (1.03 mg/kg).

scholarly journals Potential of Industrial Pineapple (Ananas comosus (L.) Merrill) By-Products as Aromatic and Antioxidant Sources

Antioxidants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1767
Author(s):  
Arantzazu Valdés García ◽  
María Isabel Domingo Martínez ◽  
Mercedes Ponce Landete ◽  
María Soledad Prats Moya ◽  
Ana Beltrán Sanahuja
Keyword(s):  
Antioxidant Capacity ◽  
Volatile Compounds ◽  
Solid Phase Microextraction ◽  
Food Industry ◽  
Solid Phase ◽  
Ananas Comosus ◽  
Antioxidant Compounds ◽  
Mass Spectrometry Detector ◽  
Food Applications ◽  
By Products

Pineapple is meanly commercially processed. However, it is a fruit that generates a high proportion of nonedible wastes, which are rich in antioxidant compounds and have a varied aromatic profile. These characteristics turn these by-products into potential agri-food waste that can be revalued and applied in different fields such as medical, pharmaceutical, or food applications. The aim of the present work was the characterization and extraction of the volatile compounds present in two pineapple by-products (peel and core) and the subsequent evaluation of their antioxidant capacity. For this purpose, the analysis of the aromatic profile of both by-products has been carried out using the headspace solid-phase microextraction technique coupled to gas chromatography with a mass spectrometry detector (HS-SPME-GC-MS). The optimization of the extraction conditions of the volatile compounds has been validated using a Box–Behnken experimental design. In addition, a quantitative analysis was carried out to determine the contents of two important volatiles in pineapple wastes, isopentyl, and ethyl acetate. Moreover, the estimation of the antioxidant capacity of the subproducts extracts was carried out using different methods All the antioxidant assays demonstrated that pineapple subproducts are rich in easily extractable antioxidants with possible applications in the food industry.

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