scholarly journals Optimization of High-Pressure Extraction Process of Antioxidant Compounds from Feteasca regala Leaves Using Response Surface Methodology

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4209
Author(s):  
Anca Becze ◽  
Vanda Liliana Babalau-Fuss ◽  
Cerasel Varaticeanu ◽  
Cecilia Roman
Keyword(s):  
Response Surface Methodology ◽  
Antioxidant Capacity ◽  
Response Surface ◽  
Total Antioxidant Capacity ◽  
Raw Materials ◽  
Extraction Process ◽  
Extraction Yield ◽  
Antioxidant Compounds ◽  
Operational Conditions ◽  
Total Antioxidant

Circular economy principles are based on the use of by-products from one operation as the raw materials in another. The aim of this work is to obtain extracts with high antioxidant capacity and resveratrol content for the superior capitalization of the biomass of Feteasca regala leaves obtained during vineyard horticultural operations in spring. In order to obtain a high-quality extract at an industrial level, an optimal extraction process is needed. Central composite design (CCD) was used for the experiment design, which contained three independent variables: the ratio of extraction solvent to solid matter, temperature (°C) and time (minutes). The evaluation of extracts was done by measuring the total antioxidant capacity of the extracts using photo-chemiluminescent techniques, and the resveratrol content using liquid chromatography. Process optimization was done using response surface methodology (RSM). Minitab software version 17.0 was used for the design of experiments and data analysis. Regression analysis showed that the model predicts 87.5% of the variation for resveratrol and 96% for total antioxidant capacity (TAC). The temperature had the biggest influence on the extraction yield. The optimal operational conditions for the extraction method applied had the following conditions: ratio e/m 2.92; 43.23 °C and 55.4 min. A maximum value of 34,623 µg ascorbic acid equivalent (AAE) /mL total antioxidant capacity and 182.4 µg/mL resveratrol content were obtained when the optimal extraction parameters where used. The values obtained in experiments proved that by using RSM an accurate model can be obtained for extraction of Feteasca regala leaves.

EXTRACTION OPTIMIZATION AND ANTIOXIDANT ACTIVITY OF PHENOLIC COMPOUNDS FROM AVOCADO PEEL

2019 ◽  
pp. 49-59
Author(s):  
Nu Linh Giang Ton ◽  
Thi Hoai Nguyen ◽  
Quoc Hung Vo
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Antioxidant Capacity ◽  
Response Surface ◽  
Total Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Quadratic Model ◽  
Total Phenolic ◽  
Total Antioxidant

Avocado peel has been considered as a potential source of natural antioxidants in which phenolics are among the most important compounds. Therefore, this study aims to optimize the extraction process of phenolics using response surface methodology and evaluate the corresponding antioxidant activity. From the quadratic model, the optimal condition was determined including the ethanol concentration 54.55% (v/v), the solvent/solute ratio 71.82/1 (mL/g), temperature 53.03 oC and extraction time 99.09 min. The total phenolic content and the total antioxidant capacity at this condition with minor modifications were 26,74 ± 0,04 (mg GAE/g DW) and 188.06 ± 1.41 (mg AAE/g DW), respectively. The significant correlation between total phenolic content and total antioxidant capacity was also confirmed. Key words: response surface methodology, central composite rotatable design, total phenolic content, total antioxidant capacity, avocado peel

scholarly journals Optimisation of the extraction process of antioxidant capacity compounds from beet (Beta vulgaris L.) stalk

2020 ◽  
Vol 41 (6) ◽  
pp. 2621-2632
Author(s):  
Emanueli Backes ◽  
◽  
Aziza Kamal Genena ◽  
Keyword(s):  
Response Surface Methodology ◽  
Free Radicals ◽  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Extraction Process ◽  
Antioxidant Compounds ◽  
Independent Variables ◽  
Potential Source ◽  
Water Ratio ◽  
Reducing Capacity

Beet is a traditional root consumed worldwide and is considered a potential source of several bioactive compounds, yet during production and commercialisation activities, its leaves and stalks are discarded. The beet residue has notable quantities of diverse phenolic compounds that are not taken advantage of it. The beet stalks (sample) were obtained from a local producer at the municipality of Marechal Cândido Rondon, Paraná state, Brazil. The optimisation of the extraction process of antioxidant capacity compounds from beet stalk was done by response surface methodology (RSM), with three independent variables (time, from 5 to 85 min; temperature, from 20 to 80 °C and solvent, from 0 to 100% of ethanol/water ratio). Extracts were evaluated for their reducing capacity, measured by the Folin–Ciocalteu method, and antioxidant capacity by the ability to scavenge DPPH and ABTS free radicals. The optimal global extraction conditions determined were 5 min, 80 °C and 50% ethanol, yielding 13.157 mg gallic acid equivalents (GAE) g–1, 21.539 mol Trolox equivalents g-1 (TE; DPPH) and 250.190 ?mol TE g–1 (ABTS). Beet stalk demonstrated to be an alternative and rich source of recovering of natural antioxidant compounds, showing higher contents when compared to other agro-industrial residues.

Antioxidant capacity of fractions of alcoholic extracts of sugarcane leaves

2013 ◽  
pp. 165-169
Author(s):  
Juliana Aparecida de Souza-Sartori ◽  
Adna Prado ◽  
Lucimara Fernanda Veiga ◽  
Nathalia Torres ◽  
Severino Matias de Alencar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Silica Gel ◽  
Plant Development ◽  
Final Stage ◽  
Total Antioxidant Capacity ◽  
Solvent Mixture ◽  
Antioxidant Compounds ◽  
Initial Stage ◽  
Total Antioxidant

Extraction of antioxidant compounds present in the top leaves of sugarcane at different stages of plant development showed that the most favorable phase for the extraction is the final stage of maturation of the plant. Flavonoid contents (5.98±0.32mg/mL) and relative antioxidant activity (AArel=257.49±16.94%) similar to the initial stage of maturation were found. Active compounds were chromatographically fractionated over silica gel and elution with solvents of increasing polarity. The antioxidant activity towards -carotene, and the sequestering activity of DPPH (1,1-diphenyl-2-picrylhydrazyl) radicals was determined. The fractions which had the highest total antioxidant capacity (TAC) were obtained from the elution of the solvent mixture: chloroform and ethanol. The results indicate that the top leaves of sugarcane are a possible source of bioactive compounds.

scholarly journals Effects of lingonberry extract on the antioxidant capacity of meat paste

2020 ◽  
Vol 8 (2) ◽  
pp. 250-258
Author(s):  
Bayana Bazhenova ◽  
Sesegma Zhamsaranova ◽  
Yuliya Zabalueva ◽  
Alexander Gerasimov ◽  
Natalya Zambulaeva
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Raw Materials ◽  
Physicochemical Characteristics ◽  
Bactericidal Effect ◽  
Biologically Active ◽  
E Coli ◽  
Anaerobic Microorganisms ◽  
Total Antioxidant ◽  
The Republic

Introduction. Modern meat scientists are currently interested in combining meat raw materials with plant ingredients to enrich products with biologically active substances of natural origin, including antioxidants. In this regard, we studied the antioxidant capacity of a dry lingonberry marc extract introduced into meat paste and analyzed its effects on the product’s color and stability during storage. Study objects and methods. Our objects of study were a dry lingonberry marc extract originating in the Republic of Buryatia, forcemeat, and a ready-made paste in a casing. The extract was obtained by water-alcohol extraction using microwave irradiation. We investigated the physicochemical characteristics of the dry extract, including its contents of phenolic compounds, benzoic acid, and antioxidants. Results and discussion. During the experiment, we analyzed the extract’s effect on the paste’s total antioxidant capacity, coloring, and shelf life. The results showed that increasing the extract’s amount from 0.1% to 0.4% changed the color of the paste from gray-brown to purple-brown, respectively, due to anthocyanins. In further tests, we used a 0.2% concentration of lingonberry extract – the optimal amount that retained the usual brown color of the paste while increasing the content of antioxidant substances. Then, we analyzed the degree of fat oxidation in the paste samples made with and without sodium lactate during storage. According to the results, the lingonberry marc extract used without the acidity regulator and with it inhibited lipid oxidation by 12.7% and 20%, respectively, by neutralizing free radicals. Finally, we tested the presence of pathogenic microorganisms in the end products. We detected no E. coli bacteria in the samples and found an inhibited growth of mesophilic anaerobic and facultative anaerobic microorganisms due to the extract’s bactericidal effect established in Our earlier studies. Conclusion. Thus, our results indicated that the dry lingonberry marc extract introduced into meat paste increased the product’s total antioxidant capacity and improved its stability during storage.

scholarly journals Discrimination and prediction of polyphenolic compounds and total antioxidant capacity in sorghum grains

2019 ◽  
Vol 27 (1) ◽  
pp. 46-53 ◽  
Author(s):  
Michela Alfieri ◽  
Giovanni Cabassi ◽  
Ephrem Habyarimana ◽  
Fabrizio Quaranta ◽  
Carlotta Balconi ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Condensed Tannins ◽  
Near Infrared ◽  
Total Phenolics ◽  
Rapid Screening ◽  
Antioxidant Compounds ◽  
Total Flavonoids ◽  
Analysis Model ◽  
Total Antioxidant

One hundred and forty-seven sorghum samples, grown in 2015 and 2017, were used to build different near infrared spectroscopic calibration models able to predict total antioxidant capacity, total phenolics, total flavonoids and condensed tannins content. Samples were separated into calibration and validation sets using a nearest neighbours algorithm. The r2pred values ranged from 0.84 (condensed tannins) to 0.95 (total phenolics), whereas the Ratio of Performance to Deviation (RPD) values ranged from 1.9 (total flavonoids) to 3.0 (total phenolics). Comparison of prediction error estimates highlighted the best models with significant differences. Model robustness was tested through a reduction of sample numbers in the calibration set; the highest robustness was found for total antioxidant capacity and total flavonoids. In addition, a partial least squares discriminant analysis model to screen the samples for their tannins level was developed and resulted in good performance; it should be useful to select tannin-free genotypes for the food industry. These models could be used for rapid screening of sorghum breeding genotypes with high antioxidant compounds.

scholarly journals Optimization of Extraction Process and the Antioxidant Activity of Phenolics from Sanghuangporus baumii

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3850
Author(s):  
Na Zheng ◽  
Yongfei Ming ◽  
Jianzhi Chu ◽  
Shude Yang ◽  
Guochao Wu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Choline Chloride ◽  
Deep Eutectic Solvent ◽  
Extraction Process ◽  
Extraction Yield ◽  
Fruiting Bodies ◽  
Factors Affecting ◽  
Medicinal Fungus

Sanghuangporus baumii, is a widely used medicinal fungus. The polyphenols extracted from this fungus exert antioxidant, anti-inflammatory, and hypoglycemic effects. In this study, polyphenols from the fruiting bodies of S. baumii were obtained using the deep eutectic solvent (DES) extraction method. The factors affecting the extraction yield were investigated at different conditions. Based on the results from single-factor experiments, response surface methodology was used to optimize the extraction conditions. The scavenging ability of the polyphenols on •OH, DPPH, and ABTS+ was determined. The results showed that the DES system composed of choline chloride and malic acid had the best extraction yield (6.37 mg/g). The optimal extraction parameters for response surface methodology were as follows: 42 min, 58 ℃, 1:34 solid–liquid (mg/mL), and water content of 39%. Under these conditions, the yield of polyphenols was the highest (12.58 mg/g). At 0.30 mg/mL, the scavenging ability of the polyphenols on •OH, DPPH, and ABTS+ was 95.71%, 91.08%, and 85.52%, respectively. Thus, the method using DES was more effective than the conventional method of extracting phenolic compounds from the fruiting bodies of S. baumii. Moreover, the extracted polyphenols exhibited potent antioxidant activity.

scholarly journals Enhancing Bioactive Antioxidants’ Extraction from “Horchata de Chufa” By-Products

Foods ◽  
2018 ◽  
Vol 7 (10) ◽  
pp. 161 ◽  
Author(s):  
Elena Roselló-Soto ◽  
Francisco Barba ◽  
Predrag Putnik ◽  
Danijela Bursać Kovačević ◽  
Jose Lorenzo ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Raw Materials ◽  
Extraction Process ◽  
Total Flavonoids ◽  
Cyperus Esculentus ◽  
Total Polyphenols ◽  
Solvent Type ◽  
Biological Compounds ◽  
Total Antioxidant ◽  
By Products

During the production of a traditional drink produced from the tubers of Cyperus esculentus L. also known as “horchata de chufa,” a high quantity of by-products are generated. These by-products are rich with valuable biological compounds, hence, there is a need to report their extraction conditions for further use in food production as raw materials. Therefore, the objective of this study was to evaluate and improve the conventional extraction process, applied for recovery of phenolic compounds, total flavonoids, and total antioxidant capacity from the by-products. Independent variables for extraction were: (i) Solvent type (mixtures of ethanol-water (v/v) at 0%, 25% and 50%); (ii) temperature (40, 50 and 60 °C), and (iii) extraction time (1, 2 and 3 h). The obtained results showed that solvent type, temperature, and time significantly influenced (p < 0.05) all investigated parameters. The highest content of total polyphenols (16.02 mg GAE/100 g of dry matter; d.m.), and total flavonoids (30.09 mg CE/100 g d.m.) was achieved by ethanol at 25% (v/v), after 3 h of extraction with temperatures of 60 °C and 50 °C, respectively. The highest value of antioxidant capacity (1759.81 µM Trolox equivalents/g d.m.) was observed with 50% aqueous ethanol (v/v), at 60 °C, and 3 h of extraction. From the obtained results, it can be concluded that the by-products of “Horchata de Chufa” are an important source of antioxidant bioactive compounds.

Characterization of antioxidant compounds of red and white rice and changes in total antioxidant capacity during processing

2007 ◽  
Vol 51 (8) ◽  
pp. 1006-1019 ◽  
Author(s):  
Franca Finocchiaro ◽  
Barbara Ferrari ◽  
Alberto Gianinetti ◽  
Chiara Dall'Asta ◽  
Gianni Galaverna ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Antioxidant Compounds ◽  
White Rice ◽  
Total Antioxidant

scholarly journals Determination of optimal parameters of basil supercritical fluid extraction by response surface methodology

2016 ◽  
pp. 193-203
Author(s):  
Snezana Filip ◽  
Senka Vidovic ◽  
Branimir Pavlic ◽  
Zoran Zekovic
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Supercritical Fluid Extraction ◽  
Supercritical Fluid ◽  
Interactive Effect ◽  
Extraction Process ◽  
Extraction Yield ◽  
Fluid Extraction ◽  
Experimental Values

The supercritical fluid extraction of aroma compounds from basil (Ocimum basilicum L.) was studied. Response surface methodology was used to optimize the parameters of the process. Full factorial design was applied to evaluate the effects of two independent variables (pressure and temperature) on the extraction yield and linalool yield. From the response surface plots, pressure and temperature exhibited independent and interactive effect on the extraction yield. The optimal conditions to obtain the highest extraction yield (1.91%) of O. basilicum were the pressure of 29.7 MPa and temperature of 59.2oC, whereas the highest yield of linalool (1.998 g?kg-1) was obtained at the pressure of 20 MPa and temperature of 40oC. The experimental values agreed with the predicted ones, indicating suitability of the response surface methodology for optimizing the extraction process.

Sign in / Sign up

Export Citation Format

Share Document