scholarly journals Fruit Quality Characterization of New Sweet Cherry Cultivars as a Good Source of Bioactive Phenolic Compounds with Antioxidant and Neuroprotective Potential

Antioxidants ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 677
Author(s):  
Fabiana Antognoni ◽  
Giulia Potente ◽  
Roberto Mandrioli ◽  
Cristina Angeloni ◽  
Michela Freschi ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Sweet Cherry ◽  
Prunus Avium ◽  
Total Anthocyanin ◽  
Sweet Cherries ◽  
Array Detection ◽  
Sweet Cherry Cultivars ◽  
New Cultivars

Sweet cherries (Prunus avium L.) are highly appreciated fruits for their taste, color, nutritional value, and beneficial health effects. In this work, seven new cultivars of sweet cherry were investigated for their main quality traits and nutraceutical value. The phytochemical profile of three classes of phenolic compounds and the antioxidant activity of the new cultivars were investigated through high-performance liquid chromatography with diode array detection (HPLC-DAD) and spectrophotometric assays, respectively, and compared with those of commonly commercialized cultivars. Cyanidine-3-O-rutinoside was the main anthocyanin in all genotypes, and its levels in some new cultivars were about three-fold higher than in commercial ones. The ORAC-assayed antioxidant capacity was positively correlated with the total anthocyanin index. The nutraceutical value of the new cultivars was investigated in terms of antioxidant/neuroprotective capacity in neuron-like SH-SY5Y cells. Results demonstrated that the new cultivars were more effective in counteracting oxidative stress and were also able to upregulate brain-derived neurotrophic factor (BDNF), a pro-survival neurotrophin, suggesting their potential pleiotropic role in counteracting neurodegenerations.

scholarly journals Phenolic Profile and Bioactive Potential of Stems and Seed Kernels of Sweet Cherry Fruit

Antioxidants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1295 ◽  
Author(s):  
Sílvia Afonso ◽  
Ivo Vaz Oliveira ◽  
Anne S. Meyer ◽  
Alfredo Aires ◽  
Maria José Saavedra ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Sweet Cherry ◽  
Antioxidant Activities ◽  
Antibacterial Activities ◽  
Gram Positive ◽  
Phenolic Profile ◽  
Bioactive Potential ◽  
Sweet Cherry Cultivars

Every year, large quantities of stems and pits are generated during sweet cherry processing, without any substantial use. Although stems are widely recognized by traditional medicine, detailed and feasible information about their bioactive composition or biological value is still scarce, as well as the characterization of kernels. Therefore, we conducted a study in which bioactivity potential of extracts from stems and kernels of four sweet cherry cultivars (Early Bigi (grown under net cover (C) and without net cover (NC)), Burlat, Lapins, and Van) were examined. The assays included antioxidant (by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic) acid (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and β-carotene-linoleic acid bleaching assays), and antibacterial activities against important Gram negative and Gram positive bacterial human isolates. Profile and individual phenolic composition of each extract were determined by High-performance liquid chromatography (HPLC) analysis. Extracts from stems of cv. Lapins and kernels of Early Bigi NC presented high levels of total phenolics, flavonoids, ortho-diphenols and saponins. Excepting for cv. Early Bigi NC, major phenolic compounds identified in stems and kernels were sakuranetin and catechin, respectively. In cv. Early Bigi NC the most abundant compounds were ellagic acid for stems and protocatechuic acid for kernels. In all extracts, antioxidant activities showed a positive correlation with the increments in phenolic compounds. Antimicrobial activity assays showed that only stem’s extracts were capable of inhibiting the growth of Gram positive isolates. This new data is intended to provide new possibilities of valorization of these by-products and their valuable properties.

scholarly journals The Profile and Content of Polyphenols and Carotenoids in Local and Commercial Sweet Cherry Fruits (Prunus avium L.) and Their Antioxidant Activity In Vitro

Antioxidants ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 534 ◽  
Author(s):  
Dominika Średnicka-Tober ◽  
Alicja Ponder ◽  
Ewelina Hallmann ◽  
Agnieszka Głowacka ◽  
Elżbieta Rozpara
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Sweet Cherry ◽  
Prunus Avium ◽  
Sweet Cherries ◽  
High Antioxidant Activity ◽  
Positive Correlations ◽  
Sweet Cherry Cultivars ◽  
Prunus Avium L

The aim of this study was to evaluate and compare the content of a number of bioactive compounds and antioxidant activity of fruits of selected local and commercial sweet cherry (Prunus avium L.) cultivars. The experiment showed that the selected cultivars of sweet cherries differ significantly in the content of polyphenolic compounds and carotenoids. The fruits of commercial sweet cherry cultivars were, on average, richer in polyphenols (the sum of phenolic compounds determined chromatographically), flavonoids, as well as anthocyanins and were characterized by higher antioxidant activity when compared to the local, traditional cultivars. In the group of the traditional sweet cherry cultivars, particular attention could be paid to Black Late cv., showing the highest antioxidant activity of fruits. In the group of commercial sweet cherry cultivars, Cordia and Sylvia fruits could be recognized as being rich in bioactive compounds with high antioxidant activity. Yellow skin cultivars were characterized by the highest concentrations of carotenoids. Strong positive correlations between the identified bioactive compounds and antioxidant activity of fruits were also found. Although different cultivars of sweet cherries show a high variability in phenolics and carotenoids profiles as well as in the antioxidant activity of fruits, they all should be, similarly to other types of cherries, recognized as a rich source of bioactive compounds with an antioxidant potential.

scholarly journals Identification and Characterization of a Novel Robigovirus Species from Sweet Cherry in Turkey

Pathogens ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 57 ◽  
Author(s):  
Kadriye Çağlayan ◽  
Vahid Roumi ◽  
Mona Gazel ◽  
Eminur Elçi ◽  
Mehtap Acioğlu ◽  
...  
Keyword(s):  
High Throughput Sequencing ◽  
Sweet Cherry ◽  
Prunus Avium ◽  
Open Reading Frames ◽  
Cherry Tree ◽  
Coding Regions ◽  
Sweet Cherries ◽  
Identification And Characterization ◽  
Reading Frames

High throughput sequencing of total RNA isolated from symptomatic leaves of a sweet cherry tree (Prunus avium cv. 0900 Ziraat) from Turkey identified a new member of the genus Robigovirus designated cherry virus Turkey (CVTR). The presence of the virus was confirmed by electron microscopy and overlapping RT-PCR for sequencing its whole-genome. The virus has a ssRNA genome of 8464 nucleotides which encodes five open reading frames (ORFs) and comprises two non-coding regions, 5′ UTR and 3′ UTR of 97 and 296 nt, respectively. Compared to the five most closely related robigoviruses, RdRp, TGB1, TGB2, TGB3 and CP share amino acid identities ranging from 43–53%, 44–60%, 39–43%, 38–44% and 45–50%, respectively. Unlike the four cherry robigoviruses, CVTR lacks ORFs 2a and 5a. Its genome organization is therefore more similar to African oil palm ringspot virus (AOPRV). Using specific primers, the presence of CVTR was confirmed in 15 sweet cherries and two sour cherries out of 156 tested samples collected from three regions in Turkey. Among them, five samples were showing slight chlorotic symptoms on the leaves. It seems that CVTR infects cherry trees with or without eliciting obvious symptoms, but these data should be confirmed by bioassays in woody and possible herbaceous hosts in future studies.

Resistance of sweet cherry cultivars to fruit surface pitting

2006 ◽  
Vol 86 (4) ◽  
pp. 1197-1202 ◽  
Author(s):  
Frank Kappel ◽  
Peter Toivonen ◽  
Sabina Stan ◽  
Darrell-Lee McKenzie
Keyword(s):  
Key Words ◽  
Sweet Cherry ◽  
Prunus Avium ◽  
Industry Standard ◽  
Sweet Cherries ◽  
Sweet Cherry Cultivars ◽  
Fruit Surface ◽  
Prunus Avium L

A recently developed technique was used to determine the susceptibility to fruit surface pitting of new sweet cherry (Prunus avium L.) cultivars and compare them to an industry standard. The cultivars tested included Bing (industry standard), Cristalina, Lapins, Sandra Rose, Santina, Skeena, Sonata, Staccato, and Sweetheart. Fruit were harvested at commercial maturity, injured, held at 1°C for 2 wk and then rated for fruit surface pitting. The cultivars Lapins, Skeena, Staccato, and Sweetheart had less pitting than Bing. Cristalina and Sonata tended to have similar levels of injury to Bing and Sandra Rose and Santina tended to have more severe pitting than Bing. Key words: Sweet cherries, cultivars, simulated pitting injury

scholarly journals Wild and Rare Self-Incompatibility Allele S17 Found in 24 Sweet Cherry (Prunus avium L.) Cultivars

2021 ◽  
Author(s):  
Agnes Kivistik ◽  
Liina Jakobson ◽  
Kersti Kahu ◽  
Kristiina Laanemets
Keyword(s):  
Sweet Cherry ◽  
Prunus Avium ◽  
Eastern European ◽  
S Locus ◽  
Sweet Cherries ◽  
S Alleles ◽  
Allele Specific ◽  
S Allele ◽  
Sweet Cherry Cultivars ◽  
Consensus Primers

AbstractThe pollination of self-incompatible diploid sweet cherry is determined by the S-locus alleles. We resolved the S-alleles of 50 sweet cherry cultivars grown in Estonia and determined their incompatibility groups, which were previously unknown for most of the tested cultivars. We used consensus primers SI-19/20, SI-31/32, PaConsI, and PaConsII followed by allele-specific primers and sequencing to identify sweet cherry S-genotypes. Surprisingly, 48% (24/50) of the tested cultivars, including 17 Estonian cultivars, carry the rare S-allele S17, which had initially been described in wild sweet cherries in Belgium and Germany. The S17-allele in Estonian cultivars could originate from ‘Leningradskaya tchernaya’ (S6|S17), which has been extensively used in Estonian sweet cherry breeding. Four studied cultivars carrying S17 are partly self-compatible, whereas the other 20 cultivars with S17 have not been reported to be self-compatible. The recommended pollinator of seven self-incompatible sweet cherries is of the same S-genotype, including four with S17-allele, suggesting heritable reduced effectiveness of self-infertility. We classified the newly genotyped sweet cherry cultivars into 15 known incompatibility groups, and we proposed four new incompatibility groups, 64–67, for S-locus genotypes S3|S17, S4|S17, S5|S17, and S6|S17, respectively, which makes them excellent pollinators all across Europe. Alternatively, the frequency of S17 might be underestimated in Eastern European populations and some currently unidentified sweet cherry S-alleles might potentially be S17.

scholarly journals Storage Characteristics of New Sweet Cherry Cultivars

HortScience ◽  
2002 ◽  
Vol 37 (1) ◽  
pp. 139-143 ◽  
Author(s):  
F. Kappel ◽  
P. Toivonen ◽  
D.-L. McKenzie ◽  
S. Stan
Keyword(s):  
Respiration Rate ◽  
Sweet Cherry ◽  
Prunus Avium ◽  
Modified Atmosphere ◽  
Fruit Firmness ◽  
Sweet Cherry Cultivars ◽  
Storage Characteristics ◽  
Fruit Surface ◽  
New Cultivars ◽  
Quality Measurements

Several sweet cherry (Prunus avium L.) cultivars were stored in air or modified-atmosphere packages (MAP) at 1 °C for 2 or 4 weeks, respectively. The new cultivars included `Santina', `Sumpaca Celeste', `Sumnue Cristalina', `Sumste Samba', `Sandra Rose', `Sumleta Sonata', and `Skeena', and the standards were `Lapins', `Sweetheart', and `Bing'. Fruit were rated for defects (stem browning, stem shrivel and fruit surface pitting), and fruit quality at harvest and after storage. Weight loss during storage was influenced by year, storage treatment, and cultivar. Stem shrivel, stem browning, and fruit surface pitting varied among cultivars and years. Generally, fruit stored in MAP had higher fruit firmness than at harvest or when stored in air. The respiration rate of fruit was lower in later than in earlier maturing cultivars, but respiration rate at harvest was not related to any of the quality measurements taken after storage.

COLDHARDINESS OF SWEET CHERRIES AS DETERMINED BY ARTIFICIAL FREEZING TESTS

1965 ◽  
Vol 45 (5) ◽  
pp. 429-435 ◽  
Author(s):  
K. Lapins
Keyword(s):  
Sweet Cherry ◽  
Prunus Avium ◽  
Primary Leaf ◽  
Department Of Agriculture ◽  
Sweet Cherries ◽  
Cold Damage ◽  
Artificial Freezing ◽  
One Year ◽  
Sweet Cherry Cultivars ◽  
The U.S

One-year-old shoots of 100 selected cherries representing the species Prunus avium (94 selections), P. fontanesiana, P. gondouini, and P. mahaleb were frozen at −35 °C, then forced in a growth chamber for 3 weeks, and visually examined for cold damage and recovery.The response of the better-known cultivars and species corresponded to their known hardiness. The hardiest seven cultivars or species included all six representatives of the species other than P. avium and one sweet cherry, the U.S. Department of Agriculture introduction Glenn Dale No. 6. The recorded differences in hardiness between sweet cherry cultivars were larger than expected. Certain numbered seedling selections were relatively hardy and may constitute a valuable source of breeding material.Bark, cambium, wood, supplemental buds, and primary leaf buds were examined for cold damage and recovery. Damage to primary leaf buds proved the most useful criterion for determining hardiness of cherry cultivars.

scholarly journals Optimization and characterization of Royal Dawn cherry (Prunus avium) phenolics extraction

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Lisard Iglesias-Carres ◽  
Anna Mas-Capdevila ◽  
Francisca Isabel Bravo ◽  
Miquel Mulero ◽  
Begoña Muguerza ◽  
...  
Keyword(s):  
Food Industry ◽  
Sweet Cherry ◽  
Prunus Avium ◽  
Specific Method ◽  
Phenolic Composition ◽  
Phenolic Profile ◽  
Beneficial Effects ◽  
Sweet Cherries ◽  
Precise Characterization

AbstractTo correlate the beneficial effects of cherry consumption with their phenolic composition, a full and precise characterization is required. However, there is not a specific method to fully extract all phenolic compounds from sweet cherries. Thus, this study aimed to optimize the extraction of sweet cherry phenolics by response surface methodology and fully characterize the phenolic profile of Royal Dawn sweet cherries by HPLC-ESI-MS/MS. Extraction conditions were evaluated and optimized to 55 °C, MeOH 72%, 12 mL/g in two extraction steps. Royal Dawn sweet cherries presented rutin as the predominant phenolic compound, unlike most sweet cherry varieties. Additionally, ethanol was evaluated as a replacement solvent, obtaining lower extraction rates, especially for anthocyanins. However, in terms of total amounts, non-anthocyanin compounds were similarly extracted. The developed methodology was fast and can be routinely used in the evaluation of the phenolic profile of sweet cherries and to produce phenolic-rich extracts for the food industry.

scholarly journals Characterization of Yew Tree (Taxus) Varieties by Fingerprint and Principal Component Analyses

2012 ◽  
Vol 7 (9) ◽  
pp. 1934578X1200700
Author(s):  
Kalle Truus ◽  
Merike Vaher ◽  
Maria Borissova ◽  
Marju Robal ◽  
Tuuli Levandi ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Principal Component ◽  
Baccatin Iii ◽  
Peak Areas ◽  
Array Detection ◽  
Using Data ◽  
Yew Tree

The composition of varieties of Taxus growing in Estonia was analyzed by capillary electrophoresis with diode array detection (CE-DAD) for the separation of phenolic compounds, and by high performance liquid chromatography-mass spectrometry (HPLC-MS) for the determination of taxoids. The main purpose of this study was the chemotaxonomic differentiation of varieties of Taxus by using data from these analyses. Fingerprints scanned at 214 nm on the basis of CE separation at pH 9.3 were used to characterize seven varieties of yew. The contents of four key taxoids (10-deacetylbaccatin, baccatin III, cephalomannine and paclitaxel) in six Taxus varieties were comparatively determined by HPLC-MS. The set of electropherograms/chromatograms of the various Taxus extracts were subjected to principal component analysis (PCA), using the peak areas of 16 phenolic compounds and 14 taxoids as characteristics. The formation of distinct clusters in accordance with botanical classification proves the suitability of PCA for differentiating varieties of Taxus.

scholarly journals Genome-Wide Identification of the Xyloglucan endotransglucosylase/Hydrolase (XTH) and Polygalacturonase (PG) Genes and Characterization of their Role in Fruit Softening of Sweet Cherry

2021 ◽  
Vol 22 (22) ◽  
pp. 12331
Author(s):  
Zefeng Zhai ◽  
Chen Feng ◽  
Yanyan Wang ◽  
Yueting Sun ◽  
Xiang Peng ◽  
...  
Keyword(s):  
Cell Wall ◽  
Sweet Cherry ◽  
Prunus Avium ◽  
Fruit Softening ◽  
Fruit Firmness ◽  
Xyloglucan Endotransglycosylase ◽  
Genome Wide ◽  
Fruit Development And Ripening ◽  
Sweet Cherry Cultivars

Fruit firmness is an important economical trait in sweet cherry (Prunus avium L.) where the change of this trait is related to cell wall degradation. Xyloglucan endotransglycosylase/hydrolase (XTH) and polygalacturonases (PGs) are critical cell-wall-modifying enzymes that occupy a crucial position in fruit ripening and softening. Herein, we identified 18 XTHs and 45 PGs designated PavXTH1-18 and PavPG1-45 based on their locations in the genome of sweet cherry. We provided a systematical overview of PavXTHs and PavPGs, including phylogenetic relationships, conserved motifs, and expression profiling of these genes. The results showed that PavXTH14, PavXTH15 and PavPG38 were most likely to participated in fruit softening owing to the substantial increment in expression during fruit development and ripening. Furthermore, the phytohormone ABA, MeJA, and ethephon significantly elevated the expression of PavPG38 and PavXTH15, and thus promoted fruit softening. Importantly, transient expression PavXTH14, PavXTH15 and PavPG38 in cherry fruits significantly reduced the fruit firmness, and the content of various cell wall components including hemicellulose and pectin significantly changed correspondingly in the transgenic fruit. Taken together, these results present an extensive analysis of XTHs and PGs in sweet cherry and provide potential targets for breeding softening-resistant sweet cherry cultivars via manipulating cell wall-associated genes.

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