scholarly journals Cashew (Anacardium occidentale L.) Nuts Counteract Oxidative Stress and Inflammation in an Acute Experimental Model of Carrageenan-Induced Paw Edema

Antioxidants ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 660 ◽  
Author(s):  
Marika Cordaro ◽  
Rosalba Siracusa ◽  
Roberta Fusco ◽  
Ramona D’Amico ◽  
Alessio Filippo Peritore ◽  
...  
Keyword(s):  
Inflammatory Cytokines ◽  
Radical Scavenging ◽  
Oxygen Free Radical ◽  
Anacardium Occidentale ◽  
Oxidative Enzymes ◽  
Paw Edema ◽  
Analgesic Effects ◽  
Carrageenan Injection ◽  
Anti Inflammatory ◽  
The Right

Background: Anacardium occidentale L. is a medicinal plant with powerful anti-oxidative and anti-inflammatory properties. Acute inflammatory events cause tissue alterations, decrease of anti-oxidative endogenous enzymes such as superoxide dismutase, catalase and glutathione, neutrophils infiltration, increase in the activities of myeloperoxidase, malondialdehyde, and pro-inflammatory release. Methods: Paw edema was induced by subplantar injection of carrageenan into the right hind paw in rats, but 30 min before a group of animals were orally treated with 100 mg/kg of cashew nuts to evaluate the anti-inflammatory and anti-oxidative response. Results: In the present work, we found that (1) cashew nuts reduced the development of carrageenan-induced paw edema limiting the formation of edema and pain; (2) cashew nuts ameliorated the diminutions of the anti-oxidative enzymes caused by carrageenan injection; (3) cashew nuts decreased myeloperoxidase malondialdehyde activity induced by carrageenan; and (4) cashew nuts acted by blocking pro-inflammatory cytokines response and nitrate/nitrite formation stimulated by carrageenan injection. Conclusions: The mechanisms of anti-inflammatory and analgesic effects exerted by cashew nuts were relevant to oxygen free radical scavenging, anti-lipid peroxidation, and inhibition of the formation of inflammatory cytokines.

scholarly journals Comparative Anti-Inflammatory and Analgesic Activities of Qurs-Saffron and Quknar Polyherbal Tablets in Mouse Model

2020 ◽  
Vol 7 (4) ◽  
pp. 199-205
Author(s):  
Muhammad Shoaib Akhtar ◽  
Abdul Malik ◽  
Haroon Arshad ◽  
Nabiha Pervez ◽  
Nadeem Irshad
Keyword(s):  
Radical Scavenging ◽  
Toxicity Testing ◽  
Paw Edema ◽  
Standard Drug ◽  
Analgesic Effects ◽  
Parametric Data ◽  
Anti Inflammatory ◽  
Analgesic Activities ◽  
Radical Scavenging Properties ◽  
One Way Anova

Objective: We aimed to study the anti-inflammatory and analgesic effects for two polyherbal tablets in animal model. Methods: Suspensions of Qurs-Saffron and Quknar polyherbal formulations (p.o.) were evaluated for their anti-inflammatory (in carrageenan-induced paw edema) and analgesic activities (in formalin-induced paw licking test) in albino mice using Ibuprofen (50 mg/kg, orally) as standard drug. Acute toxicity testing of suspensions from Qurs-Saffron and Quknar tablets was also performed at doses 1, 2 and 3 gm/kg, orally. Parametric data were evaluated by one way ANOVA followed by Tukey’s test while graphics were made using GraphPad Prism. Results: Administration of Qurs-Saffron (1000, 1500, 2000 mg/kg) and Quknar (500, 750, 1000 mg/kg) suspensions showed significant (P < 0.05) inhibition of paw edema and at later time point (after 3 hours), both suspensions showed highly significant (P < 0.001) inhibition of paw edema. In another experiment (formalin-induced paw licking test), Qurs-Saffron (2000 mg/kg) and Quknar (1000 mg/kg) showed the highly significant (P < 0.001) analgesic activity. Conclusion: Qurs-Saffron and Quknar tablets possessed anti-inflammatory and analgesic effects that were most likely due to flavonoids and phenolic compounds with free radical scavenging properties which significantly reduced inflammation and pain in treated mice and were also found safe in toxicity testing.

scholarly journals New ibuprofen derivatives with thiazolidine-4-one scaffold with improved pharmaco-toxicological profile

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Ioana-Mirela Vasincu ◽  
Maria Apotrosoaei ◽  
Sandra Constantin ◽  
Maria Butnaru ◽  
Liliana Vereștiuc ◽  
...  
Keyword(s):  
Analgesic Effect ◽  
Visceral Pain ◽  
Biological Effects ◽  
Maximum Effect ◽  
Tail Flick ◽  
Paw Edema ◽  
Cell Viability Assay ◽  
Thermal Nociception ◽  
Analgesic Effects ◽  
Anti Inflammatory

Abstract Background Aryl-propionic acid derivatives with ibuprofen as representative drug are very important for therapy, being recommended especially for anti-inflammatory and analgesic effects. On other hand 1,3-thiazolidine-4-one scaffold is an important heterocycle, which is associated with different biological effects such as anti-inflammatory and analgesic, antioxidant, antiviral, antiproliferative, antimicrobial etc. The present study aimed to evaluated the toxicity degree and the anti-inflammatory and analgesic effects of new 1,3-thiazolidine-4-one derivatives of ibuprofen. Methods For evaluation the toxicity degree, cell viability assay using MTT method and acute toxicity assay on rats were applied. The carrageenan-induced paw-edema in rat was used for evaluation of the anti-inflammatory effect while for analgesic effect the tail-flick test, as thermal nociception in rats and the writhing assay, as visceral pain in mice, were used. Results The toxicological screening, in terms of cytotoxicity and toxicity degree on mice, revealed that the ibuprofen derivatives (4a-n) are non-cytotoxic at 2 μg/ml. In addition, ibuprofen derivatives reduced carrageenan-induced paw edema in rats, for most of them the maximum effect was recorded at 4 h after administration which means they have medium action latency, similar to that of ibuprofen. Moreover, for compound 4d the effect was higher than that of ibuprofen, even after 24 h of administration. The analgesic effect evaluation highlighted that 4 h showed increased pain inhibition in reference to ibuprofen in thermal (tail-flick assay) and visceral (writhing assay) nociception models. Conclusions The study revealed for ibuprofen derivatives, noted as 4 m, 4 k, 4e, 4d, a good anti-inflammatory and analgesic effect and also a safer profile compared with ibuprofen. These findings could suggest the promising potential use of them in the treatment of inflammatory pain conditions.

Evaluation of the analgesic, anti-inflammatory, anti-oxidant, phytochemical and toxicological properties of the methanolic leaf extract of commercially processed Moringa oleifera in some laboratory animals

2015 ◽  
Vol 26 (5) ◽  
Author(s):  
Adeolu Alex Adedapo ◽  
Olufunke Olubunmi Falayi ◽  
Ademola Adetokunbo Oyagbemi
Keyword(s):  
Leaf Extract ◽  
Moringa Oleifera ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Laboratory Animals ◽  
Phytochemical Analysis ◽  
Writhing Test ◽  
Analgesic Effects ◽  
Anti Oxidant ◽  
Anti Inflammatory

AbstractThe commercially processedThe phytochemical analysis showed that the extract contained flavonoids, terpenoids, glycosides, tannins and saponins. In the acetic acid-induced writhing test, the extract significantly reduced the number of writhes at 100 and 200 mg/kg but not so much at 50 mg/kg. In the formalin-induced paw lick test, the effect was similar to that of the acetic writhing test. The analgesic effects were comparable to that of indomethacin used at 10 mg/kg. In the anti-inflammatory test, the extract reduced the formation of oedema especially at a dose of 200 mg/kg. In the anti-oxidant test, the extract was found to possess a free radical-scavenging property and is concentration related.The use of this extract for medicinal and nutritional purposes may have thus been justified; however, caution must be exercised in its use to prevent the toxic effect.

scholarly journals Antinociceptive and anti-inflammatory effects of thiamine in Long Evans rats

2016 ◽  
Vol 11 (1) ◽  
pp. 6-12
Author(s):  
Sayema Ainan ◽  
Noorzahan Begum ◽  
Taskina Ali
Keyword(s):  
Formalin Test ◽  
Late Phase ◽  
Total Duration ◽  
Thiamine Hydrochloride ◽  
Paw Edema ◽  
Long Evans ◽  
Anti Inflammatory ◽  
The Right ◽  
Post Hoc ◽  
Medical University

Background: Thiamine along with other B vitamins has been prescribed since long for treatment of various painful conditions, though individual effects of thiamine on nociception and inflammation are yet to be clearly demonstrated.Objective: To assess the effects of increasing doses of thiamine supplementation against pain and inflammation.Methods: This experimental study was conducted in the Department of Physiology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka from March 2014 to February 2015. Total twenty four male Long Evans rats weighing (200±20 gm) were treated with three different doses of thiamine hydrochloride (THCL 100, 200, 250, mg/kg/day; experimental) or normal saline (5 ml/kg/day; control) intraperitoneally for 7 consecutive days. To evaluate the thiamine’s effect on nociceptive pain, early phase (0-5 minutes) and on inflammatory pain, late phase (16-60 minutes) of the formalin test, were observed. In both phases, total frequency of jerking and total duration of flexing and licking of the right hind paw were counted after administration of subcutaneous formalin (50 ?l, 2.5%) injection. After formalin test, all the rats were also subjected to formalin induced paw edema test using a water plethysmometer to observe the antiinflammatory effect of thiamine. Statistical analysis was done by ANOVA, followed by Bonferroni post hoc test. In the interpretation of results, p?0.05 was considered as significant.Results: In formalin test, thiamine lowered frequency of jerking (after all 3 doses, in both phases) and duration of flexing and licking (200 mg/kg - p<0.001, in late phases; 250 mg/kg (p<0.001), in both phases) significantly. Additionally, thiamine lowered paw edema significantly (p<0.001) in higher 2 doses.Conclusion: This study concludes that, thiamine may have dose dependent antinociceptive and anti-inflammatory effects.Bangladesh Soc Physiol. 2016, June; 11(1): 6-12

scholarly journals Analgesic and Anti-Inflammatory Activities of Methanol Extract ofFicus pumilaL. in Mice

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Chi-Ren Liao ◽  
Chun-Pin Kao ◽  
Wen-Huang Peng ◽  
Yuan-Shiun Chang ◽  
Shang-Chih Lai ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Methanol Extract ◽  
Inflammatory Diseases ◽  
Paw Edema ◽  
Tissue Destruction ◽  
Hplc Fingerprint ◽  
Analgesic Effects ◽  
Inflammatory Mechanism ◽  
Tnf Α ◽  
Anti Inflammatory

This study investigated possible analgesic and anti-inflammatory mechanisms of the methanol extract ofFicus pumila(FPMeOH). Analgesic effects were evaluated in two models including acetic acid-induced writhing response and formalin-induced paw licking. The results showedFPMeOHdecreased writhing response in the acetic acid assay and licking time in the formalin test. The anti-inflammatory effect was evaluated by λ-carrageenan-induced mouse paw edema and histopathological analyses.FPMeOHsignificantly decreased the volume of paw edema induced by λ-carrageenan. Histopathologically,FPMeOHabated the level of tissue destruction and swelling of the edema paws. This study indicated anti-inflammatory mechanism ofFPMeOHmay be due to declined levels of NO and MDA in the edema paw through increasing the activities of SOD, GPx, and GRd in the liver. Additionally,FPMeOHalso decreased the level of inflammatory mediators such as IL-1β, TNF-α, and COX-2. HPLC fingerprint was established and the contents of three active ingredients, rutin, luteolin, and apigenin, were quantitatively determined. This study provided evidence for the classical treatment ofFicus pumilain inflammatory diseases.

scholarly journals Evaluation of Anti-Inflammatory and Analgesic Activities of Methanolic Extract of Aitchisonia Rosea in Albino Mice

2020 ◽  
Vol 8 (1) ◽  
pp. 31-36
Author(s):  
Shahid Rasool ◽  
Ambreen Malik Uttra ◽  
Mehreen Malik Uttra ◽  
Faheem Ahmed ◽  
Khizar Abbas
Keyword(s):  
Acetic Acid ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Paw Edema ◽  
Egg Albumin ◽  
Inflammatory Conditions ◽  
Albino Mice ◽  
Anti Inflammatory

Objective:  Current analysis aims at in-vivo investigation of analgesic and anti-inflammatory activities of Aitchisonia rosea methanolic extract. Methods: Albino mice (20-30 g) were used in the study. Extract at 200, 400 and 800 mg/kg doses was tested against egg albumin and carrageenan induced mice paw edema along with formalin persuaded paw licking and acetic acid prompted writhing for analgesic activity. Results: Results depicted dose reliant and significant (p<0.001) inhibition of carrageenan as well as egg albumin brought paw edema, abdominal constrictions/ hind limb stretching tempted by acetic acid and formalin instigated paw licking. Capability of crude extract to halt inflammatory and analgesic response was akin to that of ibuprofen in all models. Conclusion: The current findings support the use of Aitchisonia rosea for inflammatory conditions, which might be attributed to its previously proven high alkaloid, flavonoids, phenol, tannins content and free radical scavenging activity.

scholarly journals THE ANTI-INFLAMMATORY EFFECT OF GARDI PATCH IN CARRAGEENAN-INDUCED PAW EDEMA OF RATS

2018 ◽  
pp. 50-54 ◽  
Author(s):  
Dejidmaa B ◽  
Uuganbayar B ◽  
Erdenechimeg Ch ◽  
Chimedragchaa Ch ◽  
Munkhzul G
Keyword(s):  
Vehicle Group ◽  
Control Group ◽  
Paw Edema ◽  
Rat Paw Edema ◽  
Tnf Α ◽  
Traditional Drug ◽  
Anti Inflammatory ◽  
The Right ◽  
Rat Paw ◽  
Traditional Mongolian Medicine

Gardi -5 has been used in traditional Mongolian medicine as an analgesic and antibacterial medicine. The drug has been studied in chemical-pharmacological research. We have a new natural patch from the prepared Gardi-5. The present work was undertaken to evaluate the traditional drug Gardi patch for its anti-inflammatory activity.The method of Miho Sekiquichi was used to study acute inflammation. Rats in groups of five each were treated with vehicle, Gardi patch and Ketoprofen patch one hour prior to Carrageenan injection. 0.1 ml of 1% Carrageenan was injected into the sub plantar tissue of left hind paw of each rat. Swelling of carrageenan injected foot was measured at 0, 30, 60, 120, 180, 240, 300 mins using Plethysmometer (UGO Basile, Italy). The right hind paw was injected with 0.1 ml of vehicle.The Gardi patch significantly (p=0.000) inhibited carrageenan induced rat paw edema as compared to control group. In assay data, the TNF-α, PGE2 secretion in serum were highly elevated by carrageenan induction but administration of Gardi patch significantly reduced serum secretion of inflammatory mediators as compared to vehicle group. Gardi patch has an anti-inflammatory properties.

scholarly journals CHEMICAL ANALYSIS, ANTIOXIDANT, ANTI-INFLAMMATORY AND ANTINOCICEPTIVE EFFECTS OF ACETONE EXTRACT OF ALGERIAN SOLENOSTEMMA ARGEL (DELILE) HAYNE LEAVES

2020 ◽  
pp. 72-81
Author(s):  
DAOUIA KELTOUM BENMAAROUF ◽  
DIANA C. G. A. PINTO ◽  
BERNARD CHINA ◽  
SAFIA ZENIA ◽  
KHEIRA BOUZID BENDESARI ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Granuloma Formation ◽  
Paw Edema ◽  
Oral Toxicity ◽  
Traditional Use ◽  
Anti Inflammatory ◽  
Dose Dependent

Objective: To investigate the qualitative composition of the acetonic extract from leaves of S. argel (AESA) and their anti-inflammatory and analgesic properties in vivo. Methods: AESA profile was established by UHPLC/DAD/ESI-MS2. AESA was subjected to the acute oral toxicity study according to the OECD-420 method. Antioxydant activity of AESA was performed by DPPH radical scavenging assay. Anti-inflammatory effects of AESA were determined in two animal models: carrageenan-induced paw edema in rats and cotton pellet-induced granuloma formation in rats. Further, anti-nociceptives activities of AESA were assessed by hot plate test, acetic acid-induced abdominal writhing test and formalin test. Results: The in vivo AESA toxicity was low. AESA expresses a maximum radical scavenging activity with a IC50 value of 36,05 μg/ml. The AESA at 250 and 400 mg/kg significantly reduced carrageen an induced paw edema by 70.09% and 85.53% 6h after carrageenan injection, respectively. AESA produced significant dose-dependent anti-inflammatory effect against cotton pellets-induced granuloma formation in rats. In addition, AESA at 250 and 400 mg/kg significantly reduced acetic acid-induced writhing by 56.83 and 80.41%, respectively. Oral administration of 250 and 400 mg/kg of AESA caused a significant dose dependent anti-nociceptive effect in both neurogenic and inflammatory phases of formalin-induced licking. AESA also impacted the pain latency in the hot plat test. Conclusion: These data suggest that AESA possesses antioxidant, anti-inflammatory and anti-nociceptive effects. These results support the traditional use of S. argel to cure pain and inflammatory diseases in the Algerian Sahara.

scholarly journals Antioxidant, Anti-Inflammatory, and Analgesic Activities of Aqueous Extract of Diploknema butyracea (Roxb.) H.J. Lam Bark

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Sumit Bahadur Baruwal Chhetri ◽  
Deepa Khatri ◽  
Kalpana Parajuli
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Paw Edema ◽  
Hot Plate ◽  
Anti Inflammatory ◽  
Analgesic Activities ◽  
Radical Scavenging Assay

Diploknema butyracea (Roxb.) H.J. Lam is a multipurpose tree used by the Nepalese indigenous people for medicinal purposes such as rheumatism, asthma, and ulcer and other purposes such as cooking and lighting. However, there is no scientific evidence for the medicinal uses of this plant. The present study aimed to explore the phytochemical constituents, estimate the total phenolic content, evaluate antioxidant activity, and investigate the in vivo anti-inflammatory and analgesic activities of aqueous extract of Diploknema butyracea (Roxb.) H.J. Lam bark (ADBB). Phytochemical screening was performed using standard methods. The total phenolic content was determined using the Folin–Ciocalteu method. The in vitro antioxidant activity was determined using 2, 2-diphenyl-1-picrylhydrazyl radical scavenging assay and nitric oxide radical scavenging assay. For the in vivo studies, the plant extract was given in three different doses (50, 100, and 200 mg/kg body weight) to male albino Wistar rats. Anti-inflammatory and analgesic studies were carried out using the carrageenan-induced rat paw edema and the hot plate method, respectively. Results revealed the presence of different phytoconstituents such as flavonoids, tannins, glycosides, terpenoids, and carbohydrates together with a considerable amount of phenolic compounds. Antioxidant assays indicated the potent antioxidant activity of the plant extracts. The higher dose of D. butyracea (200 mg/kg) exhibited a maximum and significant inhibition (53.20%) of rat hind paw edema volume at 4 h and showed a greater increment in latency time (12.15 ± 1.81 sec) in the hot plate test at 120 min. The present study demonstrated the antioxidant, anti-inflammatory, and analgesic potential of ADBB, which supports its traditional medicinal use.

scholarly journals In- vitro antioxidant and In vivo Anti-inflammatory Potentials of Marantochloa Leucantha (Marantaceae) Extracts and Fractions

2019 ◽  
Vol 18 (2) ◽  
pp. 233-240
Author(s):  
Wilfred O Obonga ◽  
Charles O Nnadi ◽  
Chinonye C Chima ◽  
Sunday N Okafor ◽  
Edwin O Omeje
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Paw Edema ◽  
Frap Assay ◽  
Rat Paw Edema ◽  
Etoac Fraction ◽  
High Antioxidant Activity ◽  
Anti Inflammatory

This study evaluated the antioxidant and anti-inflammatory properties of Marantochloa leucantha (Marantaceae). The in vitro antioxidant activity of the extracts and solvent fractions was evaluated by 1,1-diphenyl- 2-picrylhydrazyl (DPPH) radical scavenging and ferric reducing antioxidant potential (FRAP) assay models and in vivo anti-inflammatory activity by the rat paw edema model. The phytochemical screening indicated the presence of tannins, terpenoids, steroids, flavonoids, reducing sugar and phenolics. The antioxidant assay showed that all the extracts exhibited high antioxidant activity comparable with ascorbic and gallic acid controls. In DPPH model, a 250 μg/ml EtOAc fraction of the leaves showed antioxidant activity of 93.9 ± 1.7 % (EC50 0.82 μg/ml) and a 1000 μg/ml of same stem fraction produced 91.9 ± 0.3 % activity (EC50 1.38 μg/ml). In the FRAP model, EtOAc fraction exhibited 31.1±0.7 and 92.0 ± 2.2 μM Fe2+/g of dried leaves and stem, respectively at 1000 μg/ml FeSO4 equivalent. The anti-inflammatory potential of the plant showed that the crude stem extract and fractions at 200 - 600 mg/kg exhibited significant (p < 0.01) dose-related inhibition of paw edema in rats. A 200 mg/kg EtOAc fraction showed 18.8 % inhibition compared to 31 % observed in diclofenac-treated rats in 2 h post albumin challenge. These findings validated the folkloric use of this plant in the treatment of diseases associated to the oxidative stress and could further provide promising lead compounds with potent antioxidant and anti-inflammatory activities Dhaka Univ. J. Pharm. Sci. 18(2): 233-240, 2019 (December)

Sign in / Sign up

Export Citation Format

Share Document