scholarly journals Antioxidant, Anti-Inflammatory, and Analgesic Activities of Aqueous Extract of Diploknema butyracea (Roxb.) H.J. Lam Bark

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Sumit Bahadur Baruwal Chhetri ◽  
Deepa Khatri ◽  
Kalpana Parajuli
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Paw Edema ◽  
Hot Plate ◽  
Anti Inflammatory ◽  
Analgesic Activities ◽  
Radical Scavenging Assay

Diploknema butyracea (Roxb.) H.J. Lam is a multipurpose tree used by the Nepalese indigenous people for medicinal purposes such as rheumatism, asthma, and ulcer and other purposes such as cooking and lighting. However, there is no scientific evidence for the medicinal uses of this plant. The present study aimed to explore the phytochemical constituents, estimate the total phenolic content, evaluate antioxidant activity, and investigate the in vivo anti-inflammatory and analgesic activities of aqueous extract of Diploknema butyracea (Roxb.) H.J. Lam bark (ADBB). Phytochemical screening was performed using standard methods. The total phenolic content was determined using the Folin–Ciocalteu method. The in vitro antioxidant activity was determined using 2, 2-diphenyl-1-picrylhydrazyl radical scavenging assay and nitric oxide radical scavenging assay. For the in vivo studies, the plant extract was given in three different doses (50, 100, and 200 mg/kg body weight) to male albino Wistar rats. Anti-inflammatory and analgesic studies were carried out using the carrageenan-induced rat paw edema and the hot plate method, respectively. Results revealed the presence of different phytoconstituents such as flavonoids, tannins, glycosides, terpenoids, and carbohydrates together with a considerable amount of phenolic compounds. Antioxidant assays indicated the potent antioxidant activity of the plant extracts. The higher dose of D. butyracea (200 mg/kg) exhibited a maximum and significant inhibition (53.20%) of rat hind paw edema volume at 4 h and showed a greater increment in latency time (12.15 ± 1.81 sec) in the hot plate test at 120 min. The present study demonstrated the antioxidant, anti-inflammatory, and analgesic potential of ADBB, which supports its traditional medicinal use.

scholarly journals In- vitro antioxidant and In vivo Anti-inflammatory Potentials of Marantochloa Leucantha (Marantaceae) Extracts and Fractions

2019 ◽  
Vol 18 (2) ◽  
pp. 233-240
Author(s):  
Wilfred O Obonga ◽  
Charles O Nnadi ◽  
Chinonye C Chima ◽  
Sunday N Okafor ◽  
Edwin O Omeje
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Paw Edema ◽  
Frap Assay ◽  
Rat Paw Edema ◽  
Etoac Fraction ◽  
High Antioxidant Activity ◽  
Anti Inflammatory

This study evaluated the antioxidant and anti-inflammatory properties of Marantochloa leucantha (Marantaceae). The in vitro antioxidant activity of the extracts and solvent fractions was evaluated by 1,1-diphenyl- 2-picrylhydrazyl (DPPH) radical scavenging and ferric reducing antioxidant potential (FRAP) assay models and in vivo anti-inflammatory activity by the rat paw edema model. The phytochemical screening indicated the presence of tannins, terpenoids, steroids, flavonoids, reducing sugar and phenolics. The antioxidant assay showed that all the extracts exhibited high antioxidant activity comparable with ascorbic and gallic acid controls. In DPPH model, a 250 μg/ml EtOAc fraction of the leaves showed antioxidant activity of 93.9 ± 1.7 % (EC50 0.82 μg/ml) and a 1000 μg/ml of same stem fraction produced 91.9 ± 0.3 % activity (EC50 1.38 μg/ml). In the FRAP model, EtOAc fraction exhibited 31.1±0.7 and 92.0 ± 2.2 μM Fe2+/g of dried leaves and stem, respectively at 1000 μg/ml FeSO4 equivalent. The anti-inflammatory potential of the plant showed that the crude stem extract and fractions at 200 - 600 mg/kg exhibited significant (p < 0.01) dose-related inhibition of paw edema in rats. A 200 mg/kg EtOAc fraction showed 18.8 % inhibition compared to 31 % observed in diclofenac-treated rats in 2 h post albumin challenge. These findings validated the folkloric use of this plant in the treatment of diseases associated to the oxidative stress and could further provide promising lead compounds with potent antioxidant and anti-inflammatory activities Dhaka Univ. J. Pharm. Sci. 18(2): 233-240, 2019 (December)

Chemical Composition of Essential Oil and in Vitro Biological Activities of Dryopteris marginalis L.

2019 ◽  
Vol 16 ◽  
Author(s):  
Md. Zahidul Islam ◽  
M. Shahinuzzaman ◽  
Raşit Fikret Yilmaz ◽  
Md. Hafizur Rahman ◽  
Yavuz Derin ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Superoxide Radical ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Organic Extracts ◽  
Radical Scavenging Assay

Dryopteris marginalis L. is a local plant in Bangladesh which is widely used as folk medicine. In this study, the chemical composition of essential oil of D. marginalis L. as well as the antioxidant activity, antibacterial activity and total phenolic content of various organic extracts were analyzed. The gas chromatography–mass spectrometry analysis was used to identify the chemical compositions. The antibacterial activity of different extracts of Dryopteris marginalis was examined against three gram-negative bacteria and two gram-positive bacteria. The antibacterial activity of different extracts was investigated using disc diffusion method and minimum inhibitory concentration showing important antibacterial activity and was measured by using broth dilution method. The antioxidant activity was determined by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and superoxide radical scavenging assay. The total phenolic portion of the extracts were ascertained by Folin-Ciocalteu in alkaline medium. Twenty-two volatile compounds were identified from the essential oil. The methanol extract exhibited significant inhibitory effect which was close to the value of standard antibiotic (Streptomycin). The zones of inhibition of different organic extracts against the tested bacteria were found in the range of (7-15) mm and the minimum inhibitory concentrations (MIC) were recorded (15.62-1000) µg/ml. The antioxidant activity of D. marginalis was (82.13+1.2) % and (59.21+0.45) % highest activity with DPPH and superoxide radical scavenging assay respectively. The methanol extract showed highest (147.43+3.1) mg/g GAE of total phenolic content. The extracts and essential oil of D. marginalis showing strong antibacterial activity can be considered as a potential source of natural medicine and the ingredient of modern therapeutic agents.

The Essential Oil of the Fruits of Ferula stenocarpa Boiss. & Hausskn: Powerful Analgesic and Anti-inflammatory Effects

2021 ◽  
Author(s):  
H. Shafaroodi ◽  
J. Asgarpanah ◽  
J. Asgarpanah
Keyword(s):  
Animal Model ◽  
Acetic Acid ◽  
Essential Oil ◽  
Acute Inflammation ◽  
Central Pain ◽  
Paw Edema ◽  
Hot Plate ◽  
Anti Inflammatory ◽  
Analgesic Activities

Ferula stenocarpa is an aromatic herb whose fruits are widely used in Persian traditional medicine as a painkiller and to relive inflammation-based disorders. Considering the higher content of essential oil in the fruits, the oil’s anti-inflammatory and analgesic activities were investigated in an animal model in vivo. The analgesic effect of Ferula stenocarpa fruits essential oil was evaluated via testing the writhing triggered by acetic acid examination and hot plate technique. Moreover, the acute anti-inflammatory effects were studied through the paw edema triggered in mice. Using 20 and 40 mg/kg of the oil revealed an analgesic impact considering the increment in the reaction time needed for the hot plate approach. Furthermore, all examined doses of the oil caused a reduction in the frequency of writhes in the mice. It was observed that the oil (40 mg/kg) followed by 10 and 20 mg/kg caused inflammatory reduction. The findings indicated that the oil may possess significant activities against acute inflammation. It had both peripheral and central pain-killing impacts. α-Pinene (43.1%) and β-pinene (22.9%) as the main constituents of the oil, can be considered as the responsible compounds to manage inflammation and pain.

scholarly journals Anti-Inflammatory and Antioxidant Activities of Cordia Dichotoma Forst

2020 ◽  
Vol 13 (4) ◽  
pp. 2093-2099
Author(s):  
Nazim Hussain ◽  
Bibhuti Bhushan Kakoti ◽  
Mithun Rudrapal ◽  
Zubaidur Rahman ◽  
Mokinur Rahman ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Radical Scavenging ◽  
Inflammatory Activity ◽  
Paw Edema ◽  
Dpph Radical ◽  
Standard Drug ◽  
Anti Inflammatory ◽  
Dpph Radical Scavenging ◽  
Radical Scavenging Assay

Cordia dichotoma Forst.has been used in the management of pain and inflammations in traditional medicine. However, the anti-inflammatory activity of the methanolic extract of C. dichotoma(MECD) bark has not been reported so far. This work was, therefore, aimed at investigating the anti-inflammatory activity of C. dichotomabark extract. The antioxidant activity was evaluated to justify the anti-inflammatory action of MECD on the basis of its radical scavenging property. The extract of C. dichotomawas obtained by Soxhlation of bark powder using methanol as solvent. The anti-inflammatory activity was determined by the carrageenan induced paw edemamodel in ratsat two different dose levels, viz., 250 and 500 mg/kg. The antioxidant activity was evaluated using DPPH radical scavenging assay. The antioxidant activity was performed in vitro by DPPH radical scavenging assay using ascorbic acid as the standard drug. In anti-inflammatory activity, maximum inhibition of edema was observed after 4 hours of experimental period. At lower test dose (250 mg/kg b.w.), the percentage inhibition of paw edema was 29.7 %, while 48.6 % inhibition of edema was observed at higher dose (500 mg/kgb.w.). The percentage inhibition of paw edema was significant relative to the control group. The standard indomethacin group also exhibited sufficiently high level of anti-inflammatory effect with 56% inhibition of paw edema at 5 mg/kg dose. In in vitroantioxidant activity, the MECD exhibited good DPPHradical scavenging activity with the IC50 vale of 62.46 µg/ml, whereas the standard drug, ascorbic acid showed comparatively more antioxidant activity with IC50 of 27.66 µg/ml.However, our study scientifically validates the folkloric claim as well as traditional uses of C. dichotomaas anti-inflammatory medication. It is suggested that the anti-inflammatory activity of C. dichotomamay be due to the antioxidant potential of phenolic phytoconstituents or plant flavonoids present in the methanolic bark extract.

In Vitro Antioxidant and In Vivo Anti-Inflammatory Activities of Ophioglossum thermale

2012 ◽  
Vol 40 (02) ◽  
pp. 279-293 ◽  
Author(s):  
Xue-Qiong Zhang ◽  
Jin-Hwa Kim ◽  
Geun-Soo Lee ◽  
Hyeong-Bae Pyo ◽  
Eun-Young Shin ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Model Systems ◽  
Total Phenolic ◽  
Etoac Fraction ◽  
Anti Inflammatory

Antioxidant fractions from Ophioglossum thermale were extracted with five different polar solvents using a Soxhlet type extractor. The total phenolic content of the extracts was determined by the Folin-Ciocalteu method. The ethyl acetate fraction of O. thermale was found to contain maximum phenolics. The dried fractions were screened for their antioxidant activity potential using in vitro model systems such as 1,1-diphenyl-2-picryl hydrazyl (DPPH), nitroblue tetrazolium (NBT) and lipid-peroxidation reduction at different concentrations. Results revealed that the EtOAc fraction exhibited the best performance in the DPPH assay, NBT assay and lipid peroxidation. All fractions showed more potent antioxidant capacity than green tea extract, a well-known antioxidant. Furthermore, the EtOAc fraction has the highest total phenolic content (475.65 mg of EGCG/g). In addition, the EtOAc fraction at 0.005% and 0.01% (g/100 ml) also significantly inhibited UVB irradiation-induced ROS generation in human dermal fibroblasts (HDFs). In a carrageenan-induced edema model, the EtOAc fraction showed an inhibitory effect (21.5%, p < 0.05) at 200 mg/kg (p.o.) after 300 min administration. Consequently, 3-O-methylquercetin (3MQ) was also isolated from the antioxidative EtOAc fraction. The data obtained using the above in vitro and in vivo tests suggest that the antioxidant activity of O. thermale and its anti-inflammatory effect on carrageenan-induced acute inflammation can be attributed to its ameliorating effect on oxidative damage, and thus it has great potential as a source for natural health products. To the best of our knowledge, this is the first report on the antioxidant activity of different polar extracts from O. thermale.

scholarly journals PHYTOCHEMICAL PROFILING, ASSESSMENT OF TOTAL PHENOLIC CONTENT, TOTAL FLAVONOID CONTENT, AND ANTIOXIDANT ACTIVITY OF ETHNOMEDICINAL PLANT, MEYNA SPINOSA FROM ASSAM

2019 ◽  
pp. 61-63
Author(s):  
REKHA BORA ◽  
SEEMA KHAKHALARY ◽  
TAPAN DUTTA
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Radical Scavenging Assay

Objective: The present study qualitative phytoconstituents examine the total phenol, total flavonoid content (TFC), and antioxidant efficiencies traditionally used plant, Meyna spinosa. Methods: Chemical profiling, estimation of total phenolic content (TPC), TFC, and antioxidant activity of ethanol extracts of M. spinosa have performed by applying standard protocols. Antioxidant activity of leaf and stem was evaluated by 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay. TPC and TFC of the plant were assessed using Folin–Ciocalteu colorimetric and aluminum colorimetric assay, respectively. Results: The findings of the study exhibit that ethanol extract of M. spinosa is proved to be the presence of phytoconstituents (7/9) such as alkaloids, terpenoids, saponins, tannins, phytosterols, flavonoids, and phenolic compounds, while carbohydrate, fixed oils, and fats are unavailable. In addition, phenolic compositions of ethanol extract of leaf and stem; 93.21±2.93 and 54.33±0.69 mg gallic acid equivalents/g extract, respectively; TFCs of leaf and stem have recorded as 61.55±1.21 and 37.55±1.28 mg quercetin equivalents/g extract, respectively. Antioxidant efficiency of both leaf and stem is tested using DPPH radical scavenging assay as IC50 20.68±0.32 and 50.99±0.56 μg/ml, respectively. Conclusion: From the above results, it has concluded that the ethanol extract of the M. spinosa leaves and stems seizes rich phytoconstituents which can be applied in food technology, drug industries, ethnopharmacological fields, etc., for the development of healthiness and to battle against negative health consequences.

scholarly journals Anti-inflammatory, antinociceptive and antioxidant activities of the hydromethanolic fraction from Annona nutans leaves

2019 ◽  
Vol 35 (5) ◽  
Author(s):  
Nathália Lucca Silva ◽  
Aline Aparecida Saldanha ◽  
Denise Brentan Silva ◽  
Carlos Alexandre Carollo ◽  
Angela Lucia Bagnatori Sartori ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Paw Edema ◽  
Hot Plate ◽  
Mato Grosso ◽  
Anti Inflammatory ◽  
Promising Source

Annona nutans (Annonaceae) is a plant species found in Bolivia, Paraguay, Argentina, and the Brazilian Cerrado, specifically in the states of Mato Grosso and Mato Grosso do Sul (Brazil). Its common names are Araticû-Mi and Araticû-Ñu. The research contributions regarding the chemical composition and biological activities of extracts from A. nutans are rare, with only four articles being found in the literature. Therefore, the present study evaluated the anti-inflammatory and antinociceptive activities of the hydromethanolic fraction (FHMeOH) using carrageenan-induced paw edema and hot-plate tests. In addition, the antioxidant activity was evaluated by DPPH radical scavenging, total phenolic, flavonoid and tannin content assays and quantification of the major metabolites by LC-MS were performed. Oral treatment with the FHMeOH (at a dose of 300 mg.kg-1) significantly reduced paw edema 2 h and 4 h after the inflammatory stimulus. The intraperitoneal (i.p.) treatment with the FHMeOH (50 and 100 mg.kg-1) proved to be most effective, and the inhibition of acute inflammation was still visible 6 h after carrageenan injection. At doses of 50 and 100 mg.kg-1 (i.p.), FHMeOH exhibits central antinociceptive effects by increasing the latency of the reaction in the hot-plate model. The FHMeOH showed antioxidant potential, and the metabolites quercetin-3-O-galactoside, quercetin-3-O-glucoside, isorhamnetin-3-O-galactoside, quercetin-3-O-β-D-apiofuranosyl-(1→2)-galactopyranoside, and chlorogenic acid were identified and quantified by LC-MS. Our results indicate, for the first time, that FHMeOH has anti-inflammatory, antinociceptive and antioxidant potential, and it is a promising source of studies for new herbal medicines

Tylophora hirsuta (Wall.) extracts ameliorates diabetes associated with inflammation in alloxan induced diabetic rats

2020 ◽  
Vol 20 ◽  
Author(s):  
Faisal Razzaque ◽  
Ali Sharif ◽  
Bushra Akhtar ◽  
Humaira Majeed Khan ◽  
Muhammad Furqan Akhtar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Diabetic Rats ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Polyphenolic Composition ◽  
Anti Inflammatory

Background: Tylophora hirsuta Wall. has long been used as traditional medicine for the treatment of diabetes. Current study is designed to evaluate the antidiabetic and anti-inflammatory activity of different extracts of aerial parts of Tylophora hirsuta. Methods: Sequential maceration was conducted to obtain extracts. Total phenolic contents were determined by FolinCiocalteau method. The antioxidant activity was assessed by DPPH free radical scavenging assay. The extracts were tested for its inhibitory activity against α-amylase in-vitro. In-vivo anti diabetic assay was conducted using alloxan induced diabetic model and OGTT was conducted on normal rats. ELISA was used to determine the proinflammatory cytokines (TNF-α and IL-6). Polyphenolic composition of the extract was analyzed using a HPLC system. Results: Aqueous extract exhibited highest total phenolic contents (985.24± 3.82 mg GAE/100 g DW), antioxidant activity (IC50 = 786.70 ± 5.23 Conclusion: These results showed that Tylophora hirsuta possess strong antidiabetic and anti-inflammatory potentials and justify its folklore use for the management of diabetes.

Total Phenolic Content and In vitro Antioxidant Activity of Endophytic Fungi Isolated from Calotropis procera L.

2019 ◽  
Vol 15 (2) ◽  
pp. 232-241 ◽  
Author(s):  
Reena Rani ◽  
Dushyant Sharma ◽  
Monika Chaturvedi ◽  
Jaya Parkash Yadav
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Endophytic Fungi ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Calotropis Procera ◽  
Total Phenolic ◽  
Free Radical Scavenging Assay ◽  
Radical Scavenging Assay

Background: Endophytic fungi are microorganisms that colonize inside the plant tissues without causing any disease symptoms. Endophytic fungi isolated from medicinal plants have emerged as an interesting source for the isolation of bioactive compounds. In this study, we selected Calotropis procera, a member of the Asclepiadaceae family commonly called ‘Aak’ to evaluate the antioxidant potential of isolated endophytic fungi. </P><P> Objective: The aim of this study was to assess the total phenolic content (TPC), antioxidant capacity by using different assay and phytochemical screening of endophytic fungi isolated from Calotropis procera (leaves, stem and root). </P><P> Method: Crude ethyl acetate extracts of 20 different endophytic fungi isolated from Calotropis procera were tested for their preliminary phytoconstituents presence, TPC estimation (by Folin–Ciocalteu colorimetric assay) and antioxidant potential [1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay, hydrogen peroxide (H2O2) free radical scavenging assay, β-carotene-linoleic free radical scavenging assay, metal chelating assay and xanthine oxidase inhibitory assay]. Results: Phenols, carbohydrates, saponins, tannins, alkaloids and flavanoids were the main phytoconstituents present in the endophytic fungi. Among the endophytes, Aspergillus nomius showed the highest TPC [72.71±1.67 µg GAE (gallic acid equivalent) /mg dry weight of fungi] and antioxidant activity for DPPH free radical scavenging assay (68.86±0.19%). A high positive linear correlation was found between TPC and xanthine oxidase inhibitory assay (R2-0.890) and between TPC and DPPH free radical scavenging assay (R2-0.839). Aspergillus and Fusarium genus species showed significant antioxidant activity by the different assay. Conclusion: The present study revealed some endophytic fungi from Calotropis procera could be a potential source of novel natural antioxidant compounds.

scholarly journals The methanolic crude extract of Garcinia lancifolia have significant pharmacological activities with potential sources of phytochemicals

2020 ◽  
Vol 8 (02) ◽  
pp. 06-12
Author(s):  
Antara Ghosh ◽  
Sujan Banik ◽  
Jamiuddin Ahmed
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Crude Extract ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Hot Plate ◽  
Plate Method ◽  
Standard Drug

Background: Garcinia lancifolia, a potential medicinal plant has significant local use to alleviate various diseases like dysentery, diarrhea, dyspepsia, and as a pain reliever but not scientifically proven at yet. The present study was aimed to evaluate the anti-diarrheal, analgesic, and antioxidant activities of crude extract of G. lancifolia. Methods: The plant parts of G. lancifolia were collected, dried, powdered, and extracted with methanol. Then the extracts were subjected to in-vivo anti-diarrheal activity by castor oil-induced method and analgesic activity by hot plate method in mice model. The Diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging and total phenolic content were demonstrated for the analysis of antioxidant activity. Results: Phytochemical screening confirmed the presence of active phytochemicals like flavonoids, saponins, alkaloids, cardiac glycoside, and terpenoids in the plant extract. The extract at doses of 200 and 400 mg/kg showed significantly a reduction of diarrheal feces by 61.161% (p 0.01) and 72.33% (p 0.001), respectively in contrast to standard drug loperamide (77.83% reduction). In the hot plate method, the crude extract (300 mg/kg) revealed significant (p 0.05) analgesia in comparison to standard aceclofenac. In an antioxidant activity test, the extract contains a moderate level of phenolic content 10.78 μg/mg of gallic acid equivalent and the antioxidant activity by using DPPH free radical scavenging showed poor antioxidant potential with an IC50 value of 148.26 μg/mL. Conclusion: The findings of this study conclude that this plant is a potential source of pharmacological actions that may be a basis for further investigation on a large scale

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