scholarly journals Erratum: Abidar, S.; et al. The Aqueous Extract from Ceratonia siliqua Leaves Protects against 6-Hydroxydopamine in Zebrafish: Understanding the Underlying Mechanism. Antioxidants 2020, 9, 304

Antioxidants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 508
Author(s):  
Sara Abidar ◽  
Razvan Stefan Boiangiu ◽  
Gabriela Dumitru ◽  
Elena Todirascu-Ciornea ◽  
Amina Amakran ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Underlying Mechanism ◽  
Ceratonia Siliqua ◽  
6 Hydroxydopamine

The authors wish to make the following correction to their paper [...]

scholarly journals The Aqueous Extract from Ceratonia siliqua Leaves Protects against 6-Hydroxydopamine in Zebrafish: Understanding the Underlying Mechanism

Antioxidants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 304 ◽  
Author(s):  
Sara Abidar ◽  
Razvan Boiangiu ◽  
Gabriela Dumitru ◽  
Elena Todirascu-Ciornea ◽  
Amina Amakran ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
High Performance ◽  
Antioxidant Activities ◽  
Memory Performance ◽  
Underlying Mechanism ◽  
Ceratonia Siliqua ◽  
Ceratonia Siliqua L ◽  
6 Hydroxydopamine

Ceratonia siliqua L. is a Mediterranean medicinal plant traditionally cultivated for its ethnopharmacological benefits, such as antidiarrheal, antidiabetic, enhance acetylcholine, antioxidant, antiatherosclerotic, and for its possible anti-neurodegenerative potential. The aim of the present study was to evaluate the chemical composition, as well as the cognitive-enhancing, anxiolytic, and antioxidant activities of the aqueous extract from C. siliqua (CsAE) leaves against 6-hydroxydopamine (6-OHDA) zebrafish Parkinson’s disease (PD) model. CsAE (0.1, 0.3, and 1 mg/L) was administered by immersion to zebrafish (Danio rerio) for eight consecutive days and one hour before each behavioral test of each day, while 6-OHDA (250 µM) treatment was supplied one day before the novel tank diving test (NTT). Qualitative and quantitative analyses were performed by the ultra-high-performance liquid chromatography (UHPLC) analysis. The memory performance was evaluated through the NTT and Y-maze tests. Additionally, the in vitro and in vivo antioxidant status and acetylcholinesterase (AChE) activity was also assessed. Our finds demonstrated that CsAE presented positive antioxidant and anti-AChE activities, which contributed to the improvement of cognitive function in the 6-OHDA zebrafish PD model.

scholarly journals Phytochemical Characterization and Evaluation of Biological Activities of Egyptian Carob Pods (Ceratonia siliqua L.) Aqueous Extract: In Vitro Study

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2626
Author(s):  
Wael Sobhy Darwish ◽  
Abada El Sayed Khadr ◽  
Maher Abd El Naby Kamel ◽  
Mabrouk A. Abd Eldaim ◽  
Ibrahim El Tantawy El Sayed ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Biologically Active ◽  
Phytochemical Analysis ◽  
Type I ◽  
Hypotonic Solution ◽  
Ceratonia Siliqua

Ceratonia siliqua (Carob) is an evergreen Mediterranean tree, and carob pods are potentially nutritive and have medicinal value. The present study was carried out to estimate the possible biological activities of phytochemical-characterized carob pod aqueous extract (CPAE). The phytochemical contents of CPAE were determined by using colorimetric methods and HPLC. In addition, the free radical scavenging properties and anti-diabetic, anti-hemolytic, and antimicrobial activities were estimated by using standardized in vitro protocols. The phytochemical analysis revealed that CPAE was rich in polyphenols, flavonoids, and alkaloids, where it contained a significant amount of gallic acid, catechin, and protocatechuic acid. Furthermore, CPAE exhibited strong antioxidant activity where it prevented the formation of 2, 2-Diphenyl-1-picryl hydrazyl, hydroxyl, and nitric oxide free radicals. Additionally, it had a potent inhibitory effect against digestive enzymes (amylase, maltase, sucrase, and lactase). Moreover, CPAE exhibited anti-Staph aureus, anti-Escherichia coli, anti-Candida albicans, and anti-herpes simplex type I virus (HSV-I). Finally, CPAE protected the erythrocyte membrane from hypotonic solution-induced hemolysis. Altogether, CPAE could be regarded as an interesting source of biologically active antioxidant, anti-diabetic, and antimicrobial preparation for a potential application in pharmaceutical and food supplement fields.

scholarly journals Echinacoside Protects against 6-Hydroxydopamine-Induced Mitochondrial Dysfunction and Inflammatory Responses in PC12 Cells via Reducing ROS Production

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Yue-Hua Wang ◽  
Zhao-Hong Xuan ◽  
Shuo Tian ◽  
Guan-Hua Du
Keyword(s):  
Mitochondrial Dysfunction ◽  
Pc12 Cells ◽  
Inflammatory Responses ◽  
Neurodegenerative Disorder ◽  
Cell Damage ◽  
Underlying Mechanism ◽  
Ros Production ◽  
Neuroprotective Effects ◽  
Reduction Activity ◽  
6 Hydroxydopamine

Parkinson’s disease (PD) is a neurodegenerative disorder characterized by progressive loss of dopaminergic (DA) neurons at the substantia nigra. Mitochondrial dysfunction and inflammatory responses are involved in the mechanism of cell damage in PD. 6-Hydroxydopamine (6-OHDA), a dopamine analog, specifically damages dopaminergic neurons. Echinacoside (ECH) is a phenylethanoid glycoside isolated from the stems ofCistanche salsa, showing a variety of neuroprotective effects in previous studies. The present study was to investigate its effect against 6-OHDA-induced neurotoxicity and possible mechanisms in PC12 cells. The results showed that 6-OHDA reduced cell viability, decreased oxidation-reduction activity, decreased mitochondrial membrane potential, and induced mitochondria-mediated apoptosis compared with untreated PC12 cells. However, echinacoside treatment significantly attenuated these changes induced by 6-OHDA. In addition, echinacoside also could significantly alleviate the inflammatory responses induced by 6-OHDA. Further research showed that echinacoside could reduce 6-OHDA-induced ROS production in PC12 cells. These results suggest that the underlying mechanism of echinacoside against 6-OHDA-induced neurotoxicity may be involve in attenuating mitochondrial dysfunction and inflammatory responses by reducing ROS production.

scholarly journals Aqueous extract of Aconitum carmichaelii Debeaux attenuates sepsis-induced acute lung injury via regulation of TLR4/NF-ΚB pathway

2020 ◽  
Vol 19 (3) ◽  
pp. 533-539
Author(s):  
Qinghai You ◽  
Jinmei Wang ◽  
Lijuan Jiang ◽  
Yuanmin Chang ◽  
Wenmei Li
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Protein Content ◽  
Aqueous Extract ◽  
Lung Tissue ◽  
Cecal Ligation ◽  
Underlying Mechanism ◽  
Enzyme Linked Immunosorbent Assay ◽  
Total Protein Content ◽  
Aconitum Carmichaelii

Purpose: To investigate the therapeutic effect of aqueous extract of Aconitum carmichaelii Debeaux (AEACD) on sepsis-induced acute lung injury (ALI), as well as explore the underlying mechanism of action. Methods: C57BL/6 mice were treated with AEACD by gavage (4.0 g/kg/day) for 5 days before cecal ligation and puncture (CLP) challenge. After 24 h, the pathological morphology of lung tissue and the biochemical parameters in bronchoalveolar lavage fluid (BALF) were determined by H&E staining and enzyme-linked immunosorbent assay (ELISA). Furthermore, the total protein content and lactate dehydrogenase (LDH) level of BALF, as well as the oxidative biomarkers (malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD)) were evaluated in the lung homogenates by ELISA assay. The levels of pro-inflammatory cytokines, TNFα, IL-1β, and IL-6, in lung tissue were measured by qRT-PCR or ELISA. Finally, key proteins in Toll-like receptor 4 (TLR4)/nuclear factor-κB (NF-κB) pathway in lung tissue were evaluated by western blot. Results: CLP challenge induced abnormal changes in the histological structures of lung tissue, lung wet-to-dry weight (W/D) ratio, protein content and LDH levels of BALF, which were remarkably reversed by AEACD. In addition, AEACD decreased MDA levels, and increased GSH levels and SOD activity in the lung tissue of CLP–treated mice (p < 0.05). Furthermore, AEACD attenuated the CLP challengeinduced upregulation of TNFα, IL-1β, and IL-6. Finally, AEACD inactivated TLR4/NF-κB pathway by upregulating IκBα and downregulating TLR4 and phosphorylated-p65 levels. Conclusion: AEACD administration protects mice against sepsis-induced ALI through the regulation of oxidative stress and inflammatory responses in lung tissues. The underlying mechanism occurs by inhibiting TLR4/NF-κB signaling pathway. Keywords: Aconitum carmichaelii Debeaux, Acute lung injury, Sepsis, TLR4, NF-κB

Carob pods (Ceratonia siliqua L.) inhibit human neutrophils myeloperoxidase and in vitro ROS-scavenging activity

RSC Advances ◽  
2015 ◽  
Vol 5 (102) ◽  
pp. 84207-84215 ◽  
Author(s):  
Kaïs Rtibi ◽  
Mohamed Amine Jabri ◽  
Slimen Selmi ◽  
Abdelaziz Souli ◽  
Hichem Sebai ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Human Neutrophils ◽  
Scavenging Activity ◽  
Ceratonia Siliqua ◽  
Ros Scavenging ◽  
Ceratonia Siliqua L ◽  
Chromatographic Profiles

Chromatographic profiles of aqueous extract of carob pods ((A) pulp and (B) seeds).

Inhibition of gastric acid secretion by a standardized aqueous extract of Cecropia glaziovii Sneth and underlying mechanism

Phytomedicine ◽  
2008 ◽  
Vol 15 (6-7) ◽  
pp. 462-469 ◽  
Author(s):  
C. Souccar ◽  
R.M. Cysneiros ◽  
M.M. Tanae ◽  
L.M.B. Torres ◽  
M.T.R. Lima-Landman ◽  
...  
Keyword(s):  
Acid Secretion ◽  
Gastric Acid Secretion ◽  
Gastric Acid ◽  
Aqueous Extract ◽  
Underlying Mechanism

scholarly journals Effect of Ceratonia siliqua Methanolic Extract and 6-hydroxydopamine on Memory Impairment and Oxidative Stress in Zebrafish (Danio rerio) Model

2019 ◽  
Vol 69 (12) ◽  
pp. 3545-3548
Author(s):  
Gabriela Dumitru ◽  
Sara Abidar ◽  
Mohamad Nhiri ◽  
Lucian Hritcu ◽  
Razvan Stefan Boiangiu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Danio Rerio ◽  
Short Term Memory ◽  
Methanolic Extract ◽  
Soluble Sugars ◽  
Public Health Problem ◽  
Major Public Health Problem ◽  
Ceratonia Siliqua ◽  
Ceratonia Siliqua L ◽  
6 Hydroxydopamine

The richness in soluble sugars and polyphenols, especially condensed tannins, vitamins, minerals and other phytochemicals, recommends Ceratonia siliqua L. species for use in therapeutic practice, especially in terms of neurotoxicity - a major public health problem. Neurodegenerative disorders arise from various metabolic dysfunctions or from the administration of chemicals such as 6-hydroxydopamine (6-OHDA), a catecholaminergic neurotoxin that induces oxidative stress and, implicitly, cellular apoptosis. The results obtained after the administration of the methanolic extract of carob in Danio rerio highlights a phenomenon of prevention of short-term memory deficits, as well as a significant antioxidant activity.

An aqueous extract of the Anogeissus leiocarpus bark (AEAL) induces the endothelium-dependent relaxation of porcine coronary artery rings involving predominantly nitric oxide

2018 ◽  
Vol 29 (6) ◽  
pp. 599-608 ◽  
Author(s):  
Lazare Belemnaba ◽  
Sylvin Ouédraogo ◽  
Mathieu Nitiéma ◽  
Thierry Chataigneau ◽  
Innocent Pierre Guissou ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Coronary Artery ◽  
Aqueous Extract ◽  
Underlying Mechanism ◽  
Pi3 Kinase ◽  
Dependent Manner ◽  
Potent Inducer ◽  
Porcine Coronary Artery ◽  
Vasorelaxant Effect ◽  
Anogeissus Leiocarpus

Abstract Background: Anogeissus leiocarpus is a Sahel tree traditionally used by the residents of Burkina Faso for its antihypertensive properties. In this study, experiments were conducted to evaluate whether an aqueous extract of the Anogeissus leiocarpus (AEAL) trunk bark induces a vasorelaxant effect on porcine coronary artery rings and to investigate the underlying mechanism. Methods: AEAL-induced relaxations were assessed using porcine coronary artery rings suspended in organ chambers. The phosphorylation levels of Src, Akt and endothelial nitric oxide synthase (eNOS) were assessed in a primary endothelial cell culture by Western blot. The reactive oxygen species (ROS) formation was assessed using dihydroethidine. Results: In porcine coronary artery rings, AEAL at 0.1–300 μg/mL induced endothelium-dependent relaxations, which were inhibited in the presence of inhibitors of nitric oxide (NO) and the endothelium-derived hyperpolarization pathways. Moreover, the AEAL-induced NO-mediated relaxations were significantly reduced by the inhibitors of Src and PI3-kinase as well as by the membrane-permeant ROS scavengers. In cultured porcine coronary artery endothelial cells, treatment with AEAL is associated with an intracellular generation of ROS. Moreover, the AEAL induced the phosphorylations of Akt (Ser473), eNOS (Ser1177) and a transient phosphorylation of Src (Ser17) in a time-dependent manner. Conclusions: These findings indicate that AEAL is a potent inducer of endothelium-dependent NO-mediated relaxations in porcine coronary arteries through the redox-sensitive Src/PI3-kinase/Akt pathway-dependent activation of eNOS.

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