Carob pods (Ceratonia siliqua L.) inhibit human neutrophils myeloperoxidase and in vitro ROS-scavenging activity

RSC Advances ◽  
2015 ◽  
Vol 5 (102) ◽  
pp. 84207-84215 ◽  
Author(s):  
Kaïs Rtibi ◽  
Mohamed Amine Jabri ◽  
Slimen Selmi ◽  
Abdelaziz Souli ◽  
Hichem Sebai ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Human Neutrophils ◽  
Scavenging Activity ◽  
Ceratonia Siliqua ◽  
Ros Scavenging ◽  
Ceratonia Siliqua L ◽  
Chromatographic Profiles

Chromatographic profiles of aqueous extract of carob pods ((A) pulp and (B) seeds).

scholarly journals The Aqueous Extract from Ceratonia siliqua Leaves Protects against 6-Hydroxydopamine in Zebrafish: Understanding the Underlying Mechanism

Antioxidants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 304 ◽  
Author(s):  
Sara Abidar ◽  
Razvan Boiangiu ◽  
Gabriela Dumitru ◽  
Elena Todirascu-Ciornea ◽  
Amina Amakran ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
High Performance ◽  
Antioxidant Activities ◽  
Memory Performance ◽  
Underlying Mechanism ◽  
Ceratonia Siliqua ◽  
Ceratonia Siliqua L ◽  
6 Hydroxydopamine

Ceratonia siliqua L. is a Mediterranean medicinal plant traditionally cultivated for its ethnopharmacological benefits, such as antidiarrheal, antidiabetic, enhance acetylcholine, antioxidant, antiatherosclerotic, and for its possible anti-neurodegenerative potential. The aim of the present study was to evaluate the chemical composition, as well as the cognitive-enhancing, anxiolytic, and antioxidant activities of the aqueous extract from C. siliqua (CsAE) leaves against 6-hydroxydopamine (6-OHDA) zebrafish Parkinson’s disease (PD) model. CsAE (0.1, 0.3, and 1 mg/L) was administered by immersion to zebrafish (Danio rerio) for eight consecutive days and one hour before each behavioral test of each day, while 6-OHDA (250 µM) treatment was supplied one day before the novel tank diving test (NTT). Qualitative and quantitative analyses were performed by the ultra-high-performance liquid chromatography (UHPLC) analysis. The memory performance was evaluated through the NTT and Y-maze tests. Additionally, the in vitro and in vivo antioxidant status and acetylcholinesterase (AChE) activity was also assessed. Our finds demonstrated that CsAE presented positive antioxidant and anti-AChE activities, which contributed to the improvement of cognitive function in the 6-OHDA zebrafish PD model.

Ceratonia siliqua leaves exert a strong ROS-scavenging effect in human neutrophils, inhibit myeloperoxydase in vitro and protect against intestinal fluid and electrolytes secretion in rats

RSC Advances ◽  
2016 ◽  
Vol 6 (70) ◽  
pp. 65483-65493 ◽  
Author(s):  
Kaïs Rtibi ◽  
Mohammed-Amine Jabri ◽  
Slimen Selmi ◽  
Hichem Sebai ◽  
Mohamed Amri ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Human Neutrophils ◽  
Physical Factors ◽  
Ceratonia Siliqua ◽  
Intestinal Fluid ◽  
Ros Scavenging ◽  
Scavenging Effect ◽  
Increased Risk ◽  
And Oxidative Stress

Chronic inflammation and oxidative stress are induced by biological, chemical and physical factors which are, in turn, associated with an increased risk of several human diseases.

scholarly journals Elicited ROS Scavenging Activity, Photoprotective, and Wound-Healing Properties of Collagen-Derived Peptides from the Marine Sponge Chondrosia reniformis

Marine Drugs ◽  
2018 ◽  
Vol 16 (12) ◽  
pp. 465 ◽  
Author(s):  
Marina Pozzolini ◽  
Enrico Millo ◽  
Caterina Oliveri ◽  
Serena Mirata ◽  
Annalisa Salis ◽  
...  
Keyword(s):  
Wound Healing ◽  
Marine Sponge ◽  
Biological Effects ◽  
Marine Sponges ◽  
In Vitro Toxicity ◽  
Scavenging Activity ◽  
Ros Scavenging ◽  
General Chemical ◽  
Collagen Hydrolysates

Recently, the bioactive properties of marine collagen and marine collagen hydrolysates have been demonstrated. Although there is some literature assessing the general chemical features and biocompatibility of collagen extracts from marine sponges, no data are available on the biological effects of sponge collagen hydrolysates for biomedical and/or cosmetic purposes. Here, we studied the in vitro toxicity, antioxidant, wound-healing, and photoprotective properties of four HPLC-purified fractions of trypsin-digested collagen extracts—marine collagen hydrolysates (MCHs)—from the marine sponge C. reniformis. The results showed that the four MCHs have no degree of toxicity on the cell lines analyzed; conversely, they were able to stimulate cell growth. They showed a significant antioxidant activity both in cell-free assays as well as in H2O2 or quartz-stimulated macrophages, going from 23% to 60% of reactive oxygen species (ROS) scavenging activity for the four MCHs. Finally, an in vitro wound-healing test was performed with fibroblasts and keratinocytes, and the survival of both cells was evaluated after UV radiation. In both experiments, MCHs showed significant results, increasing the proliferation speed and protecting from UV-induced cell death. Overall, these data open the way to the use of C. reniformis MCHs in drug and cosmetic formulations for damaged or photoaged skin repair.

scholarly journals Phytochemical Characterization and Evaluation of Biological Activities of Egyptian Carob Pods (Ceratonia siliqua L.) Aqueous Extract: In Vitro Study

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2626
Author(s):  
Wael Sobhy Darwish ◽  
Abada El Sayed Khadr ◽  
Maher Abd El Naby Kamel ◽  
Mabrouk A. Abd Eldaim ◽  
Ibrahim El Tantawy El Sayed ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Biologically Active ◽  
Phytochemical Analysis ◽  
Type I ◽  
Hypotonic Solution ◽  
Ceratonia Siliqua

Ceratonia siliqua (Carob) is an evergreen Mediterranean tree, and carob pods are potentially nutritive and have medicinal value. The present study was carried out to estimate the possible biological activities of phytochemical-characterized carob pod aqueous extract (CPAE). The phytochemical contents of CPAE were determined by using colorimetric methods and HPLC. In addition, the free radical scavenging properties and anti-diabetic, anti-hemolytic, and antimicrobial activities were estimated by using standardized in vitro protocols. The phytochemical analysis revealed that CPAE was rich in polyphenols, flavonoids, and alkaloids, where it contained a significant amount of gallic acid, catechin, and protocatechuic acid. Furthermore, CPAE exhibited strong antioxidant activity where it prevented the formation of 2, 2-Diphenyl-1-picryl hydrazyl, hydroxyl, and nitric oxide free radicals. Additionally, it had a potent inhibitory effect against digestive enzymes (amylase, maltase, sucrase, and lactase). Moreover, CPAE exhibited anti-Staph aureus, anti-Escherichia coli, anti-Candida albicans, and anti-herpes simplex type I virus (HSV-I). Finally, CPAE protected the erythrocyte membrane from hypotonic solution-induced hemolysis. Altogether, CPAE could be regarded as an interesting source of biologically active antioxidant, anti-diabetic, and antimicrobial preparation for a potential application in pharmaceutical and food supplement fields.

Assessment in vitro of the genotoxicity, antigenotoxicity and antioxidant of Ceratonia siliqua L. extracts in murine leukaemia cells L1210 by comet assay

2016 ◽  
Vol 77 ◽  
pp. 117-124 ◽  
Author(s):  
Aïcha Sassi ◽  
Ines Bouhlel ◽  
Nadia Mustapha ◽  
Imen Mokdad-Bzeouich ◽  
Fadwa Chaabane ◽  
...  
Keyword(s):  
Comet Assay ◽  
Ceratonia Siliqua ◽  
Ceratonia Siliqua L

scholarly journals Evaluation Of In Vitro Antioxidant, Phytochemical and Gc-Ms Analysis of Aqueous Extract of Solanum Dasyphyllum Fruits

2021 ◽  
Vol 7 (4) ◽  
pp. 19-25
Author(s):  
Otor Matthew ◽  
◽  
Tosin Fabunmi ◽  
Ofikwu Idoko ◽  
Bakare Ebun ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Aqueous Extract ◽  
Cardiac Glycosides ◽  
Antioxidant Properties ◽  
Antioxidant Property ◽  
Scavenging Activity ◽  
Fruit Extract ◽  
Ms Analysis

Phytochemicals, antioxidant and the bioactive compounds present in the fruits of Solanum dasyphullum was investigated. Several phytochemicals such as saponins, tannins, alkaloids, flavonoids, steroids and cardiac glycosides were detected. A total of 172 compounds were identified by GC-MS. Phenols, spermine, 2, 5- dimethylfuran and 2-methyl-1-hexadecanol were all identified. These compounds are known to have antioxidant property which may be responsible for the antioxidant property of the fruit extract. It was observed that the fruits extract exhibit antioxidant properties. The scavenging activity increases with the concentration of the extract with the following percentage scavenging activity; 200µg(90%),133µg (78%), 100 µg(69%), 67 µg(48), 33 µg(26.7%), 20 µg(18), 7 µg(7%). Compounds identified in the fruits of Solanum dasyphylum can be isolated and used as an antioxidant. Keywords: Solanum dasyphylum, oxidation, antioxidant and extracts.

Chemical composition and antioxidant activity of Carob pulp (Ceratonia Siliqua L.) extracts

2015 ◽  
Vol 5 (2) ◽  
pp. 76-83
Author(s):  
Yasmina Mokhtaria Boufadia ◽  
Manel Elaoufi ◽  
Fatiha Tabet ◽  
Mohamed Benali ◽  
Ali Riazi
Keyword(s):  
Antioxidant Activity ◽  
Food Additives ◽  
Ethanolic Extract ◽  
Natural Food ◽  
Ceratonia Siliqua ◽  
Total Blood ◽  
Pre Treatment ◽  
Ceratonia Siliqua L

  The role of polyphenols and flavonoids in prevention of cellular damages carried out with the oxidative stress is well documented. In the present experiment, we determined the polyphenols and flavonoids concentrations of three extracts (ethanol 70%: V/V, methanol 80%: V/V and distilled water extracts) of carob pulp mature (Ceratonia siliqua L.) and their in vitro and in vivo antioxidant activity. A significant dose-dependent anti-free radical activ-ity of ethanolic extract of carob (EEC) was related to the highest polyphenol content (44.74 mg QE/mL) and to the in vitro antioxidant activity (EC50 = 0.28 mg/mL) of this extract. LC-MS /MS analysis of the EEC have shown the presence of several phenolic compounds as well as many types of flavonoids. The in vivo experiment brought out the significant effect of the daily EEC oral pre-treatment of rats, 30 min before AlCl3 administration for 28 d on the improvement of the total blood antioxidant capacity. These results suggest that the carob pulp contain many substances having in vitro and in vivo antioxidant effects, and which could be used as natural food additives in order to preserve food quality.

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