scholarly journals High-Pressure Extraction of Antioxidant-Rich Fractions from Shrubby Cinquefoil (Dasiphora fruticosa L. Rydb.) Leaves: Process Optimization and Extract Characterization

Antioxidants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 457
Author(s):  
Michail Syrpas ◽  
Kiran Subbarayadu ◽  
Vaida Kitrytė ◽  
Petras Rimantas Venskutonis
Keyword(s):  
High Pressure ◽  
Extraction Process ◽  
Scavenging Capacity ◽  
Wide Range ◽  
Pressure Extraction ◽  
Phenolic Substances ◽  
Dpph Scavenging ◽  
Solid Liquid ◽  
Dasiphora Fruticosa

Dasiphora fruticosa (basionym Potentilla fruticosa) is a shrub, known in traditional medicine for centuries. Due to the wide range of pharmacological effects, interest and applications of D. fruticosa extracts are continually increasing; however, reports on optimization of extraction conditions are scarce. Herein, a multi-step high-pressure extraction process with increasing polarity solvents was developed to isolate valuable fractions from D. fruticosa leaves. Supercritical CO2 extraction recovered 2.46 g/100 g of lipophilic fraction, rich in polyunsaturated fatty acids. Further, pressurized liquid extractions (PLE) with acetone, ethanol, and water were applied to obtain antioxidant-rich higher polarity extracts. Under optimized PLE conditions, the cumulative polar fraction yield was 29.98 g/100 g. Ethanol fraction showed the highest yield (15.3 g/100 g), TPC values (148.4 mg GAE/g), ABTS•+, and DPPH• scavenging capacity (161.1 and 151.8 mg TE/g, respectively). PLE was more efficient than conventional solid–liquid extraction in terms of extraction time, extract yields, and in vitro antioxidant capacity. Phytochemical characterization of PLE extracts by UPLC-Q-TOF-MS revealed the presence of hyperoside, ellagic acid, among other health beneficial phenolic substances. Τhis study highlights the potential of high-pressure extraction techniques to isolate antioxidant-rich fractions from D. fruticosa leaves with multipurpose applications, including the prevention and treatment of chronic diseases.

Enhancement of Anticancer Activity of Acer mono by High Pressure Extraction Process

2009 ◽  
Vol 38 (9) ◽  
pp. 1243-1252 ◽  
Author(s):  
Myoung-Hoon Jeong ◽  
Seung-Seop Kim ◽  
Ji-Hye Ha ◽  
Ling Jin ◽  
Hak-Ju Lee ◽  
...  
Keyword(s):  
High Pressure ◽  
Anticancer Activity ◽  
Extraction Process ◽  
Pressure Extraction ◽  
Acer Mono

scholarly journals Stable Isolation of Phycocyanin from Spirulina platensis Associated with High-Pressure Extraction Process

2013 ◽  
Vol 14 (1) ◽  
pp. 1778-1787 ◽  
Author(s):  
Yong Seo ◽  
Woo Choi ◽  
Jong Park ◽  
Jin Park ◽  
Kyung-Hwan Jung ◽  
...  
Keyword(s):  
High Pressure ◽  
Spirulina Platensis ◽  
Extraction Process ◽  
Pressure Extraction

scholarly journals Phenolic acids and antioxidant properties of barley wholegrain and pearling fractions

2012 ◽  
Vol 21 (2) ◽  
pp. 118-131 ◽  
Author(s):  
Tamer Gamel ◽  
El-Sayed M Abdel-Aal
Keyword(s):  
Phenolic Acids ◽  
Functional Foods ◽  
Antioxidant Properties ◽  
Natural Antioxidants ◽  
Ldl Oxidation ◽  
Total Phenolic ◽  
Inhibitory Effects ◽  
Scavenging Capacity ◽  
Wide Range

Demand for functional foods that offer therapeutic and disease-prevention properties is globally increasing. Barley presents an opportunity in this regard due to its high antioxidants and dietary fiber contents. Barley wholegrain and milling fractions were investigated in terms of phenolic acids composition, scavenging capacity against DPPH and ABTS radicals and inhibition of human LDL oxidation in vitro. Ferulic and p-coumaric were the primary phenolic acids in wholegrain and pearled fractions accounting for 43-97% and 2-55% of total phenolic acids, respectively. This wide range demonstrates significant differences among barley wholegrain or pearled products. Antioxidant capacities also varied significantly among barleys with the hulless cultivars tested exhibiting the greatest. The outer layers fraction showed higher scavenging capacity against ABTS and DPPH radicals and inhibitory effects toward LDL oxidation compared with the endosperm fractions. The results suggest that the outer layer and hull fractions would potentially provide promising sources of natural antioxidants.

scholarly journals Enhancement of Skin Immune Activities of Spirulina maxima by High Pressure Extraction Process

2010 ◽  
Vol 32 (2) ◽  
pp. 157-164 ◽  
Author(s):  
Sung-Ho Oh ◽  
Do-Hyung Kang ◽  
Woon-Yong Choi ◽  
Yong-Chang Seo ◽  
Soo-Jin Heo ◽  
...  
Keyword(s):  
High Pressure ◽  
Extraction Process ◽  
Spirulina Maxima ◽  
Pressure Extraction

Non‐conventional high‐pressure extraction process: A comparative study for astaxanthin recovery from Xanthophyllomyces dendrorhous

2021 ◽  
Author(s):  
Alejandro Torres‐Haro ◽  
Melchor Arellano‐Plaza ◽  
Juan C. Mateos‐Díaz ◽  
Hugo Espinosa‐Andrews ◽  
Gustavo A. Castillo‐Herrera
Keyword(s):  
High Pressure ◽  
Comparative Study ◽  
Extraction Process ◽  
Xanthophyllomyces Dendrorhous ◽  
Pressure Extraction

Optimization of Ultra-High Pressure Extraction Process of Polysaccharides from Dendrobium Candidum by Response Surface Method

2012 ◽  
Vol 550-553 ◽  
pp. 1796-1800 ◽  
Author(s):  
Xue Tao ◽  
Yao Zhan ◽  
Qiu Yan Zhou ◽  
Feng Qin Feng ◽  
Yong Yu
Keyword(s):  
High Pressure ◽  
Response Surface ◽  
Response Surface Method ◽  
Extraction Process ◽  
Holding Time ◽  
Solid Ratio ◽  
Ultra High Pressure ◽  
Surface Method ◽  
Pressure Extraction ◽  
Dendrobium Candidum

Ultra-high pressure extraction technique was used to extract polysaccharides from Dendrobium candidum. Extraction pressure, pressure holding time and liquid-solid ratio were found to have a significant influence on the polysaccharides extraction and were optimized by the response surface method (RSM). The obtained optimum conditions were: extraction pressure 445.28MPa, pressure holding time 6.7 minutes and liquid-solid ratio 237.91ml/g. The maximum polysaccharide yield could reach 596.75mg/g under these conditions. The ultra-high pressure extraction process was a suitable method for its higher yield and shorter extraction time compared to the water-heating extraction.

New Electrochemical Techniques for Probing Phase Transfer Dynamics at Dental Interfaces in Vitro

1997 ◽  
Vol 11 (4) ◽  
pp. 548-559 ◽  
Author(s):  
P.R. Unwin ◽  
J.V. Macpherson ◽  
M.A. Beeston ◽  
N.J. Evans ◽  
D. Littlewood ◽  
...  
Keyword(s):  
Phase Transfer ◽  
Electrochemical Techniques ◽  
Transfer Reactions ◽  
Liquid Interfaces ◽  
Atomic Force ◽  
Wide Range ◽  
Tissue Surfaces ◽  
Solid Liquid ◽  
Electrochemical Microscopy

Phase transfer reactions such as dissolution, precipitation, sorption, and desorption are important in a wide range of processes on dental hard tissue surfaces. An overview is provided of several new complementary electrochemical techniques which are capable of probing the dynamics of such processes at solid/liquid interfaces from millimeter- to nanometer-length scales, with a variable time resolution down to the sub-millisecond level. Techniques considered include channel flow methods with electrochemical detection, which allow reactions at solid/liquid interfaces to be studied under well-defined and calculable mass transport regimes. Scanning electrochemical microscopy allows the chemical activity of interfaces to be mapped at higher spatial and temporal resolutions. This technique, which utilizes a scanning ultramicroelectrode, has been used extensively for the study of dissolution processes of ionic crystals, as well as in imaging the action of fluid-flow-blocking agents on dentin surfaces, which act via precipitation. So that interfaces at the nanometer level can be probed, an integrated electrochemical-atomic force microscope has been developed which enables the local solution conditions to be controlled electrochemically while topographical changes are mapped simultaneously.

scholarly journals Extraction Process, Component Analysis, and In Vitro Antioxidant, Antibacterial, and Anti-Inflammatory Activities of Total Flavonoid Extracts from Abutilon theophrasti Medic. Leaves

2018 ◽  
Vol 2018 ◽  
pp. 1-17 ◽  
Author(s):  
Chunlian Tian ◽  
Peng Zhang ◽  
Caixia Yang ◽  
Xiang Gao ◽  
Hong Wang ◽  
...  
Keyword(s):  
Radical Scavenging ◽  
Extraction Process ◽  
Raw 264.7 ◽  
Raw 264.7 Macrophages ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Anti Inflammatory ◽  
Flavonoid Fraction ◽  
Process Component

The flavonoid fraction was extracted from the leaves of Abutilon theophrasti Medic., which are usually used as a traditional Chinese herbal medicine for the treatment of inflammation and joint pain. The current study focused on the extraction process, component analysis, and in vitro antioxidant, antibacterial, and anti-inflammatory activities of the flavonoid fraction as a part of ongoing research on bioactive substances from natural plant sources. This study evaluated the antioxidant activities via assays of DPPH radical scavenging capacity, ABTS radical scavenging capacity, and reducing power and investigated inhibitory activities against Escherichia coli, Salmonella, Staphylococcus aureus, and Streptococcus. Moreover, the inflammatory activity of the flavonoid fraction was estimated by measurement of the content of tumor necrosis factor alpha, interleukin-1-beta, interleukin-6, interleukin-10, nitric oxide, and cyclooxygenase-2 and the gene expression levels of several inflammation markers, such as inducible nitric oxide synthase and cyclooxygenase-2, in RAW 264.7 macrophages after LPS treatment. In addition, the underlying anti-inflammatory mechanisms, that is, the activation of nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways, were also revealed from the gene and protein expression levels. Taken together, these results suggested that the flavonoid fraction might exert in vitro antioxidant, antibacterial, and anti-inflammatory effects on LPS-stimulated RAW 264.7 macrophages and will be potentially useful as an adjuvant treatment for oxidative stress and bacterial and inflammatory diseases.

scholarly journals Investigation of the modulatory role of Bulgarian Mentha Piperita L. oil against radiation-induced oxidative disorders and antioxidant status In Vitro

2018 ◽  
Vol 16 (3) ◽  
pp. 177-187
Author(s):  
Y. Karamalakova
Keyword(s):  
Antioxidant Status ◽  
Radical Scavenging ◽  
Mentha Piperita ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Radiation Induced ◽  
Dpph Scavenging ◽  
Investigation Report

This investigation report on the free radical-scavenging and radio-modulatory properties of Bulgarian M. piperita L. oil. The chemically pure oil (97 %) was tested alone and after expositer to ultraviolet radiation (290-320 nm) and gamma radiation at doses of 5, 10, 20 and 30 Gy. The in vitro antioxidant status studied by different spectrophotometrically methods of M. piperita alone was found to be lower compared to UVB and 10 Gy radiated oil. By direct EPR spectroscopy, single almost symmetrical EPR signals were registered in oil alone (g= 2.00492 ± 0.0003), UVB (g= 2.00586 ± 0.0002) and 10 Gy irradiated (g= 2.0019 ± 0.0002) samples. Antioxidant activity and radical scavenging capacity of M. piperita oil alone and after radiation was also evaluated by the DPPH test. The intensities of the EPR signals of M. piperita oil alone, and irradiated samples demonstrated the possible formation of stable radical structures. The EPR spectra considerably higher after radiation, and excellent DPPH scavenging capacity exhibited by M. piperita indicate good antioxidant potential, radio-modulatory activity and prevent effectively radiation–induced oxidative disorders.

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