scholarly journals Assessment of Antioxidants in Selected Plant Rootstocks

Antioxidants ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 209 ◽  
Author(s):  
Samuel Magnus ◽  
Filip Gazdik ◽  
Naser A. Anjum ◽  
Eliska Kadlecova ◽  
Zuzana Lackova ◽  
...  

The service tree (Sorbus domestica) is a wild fruit tree with immense medicinal and industrial value. This study aimed at determining the four major groups of antioxidants (flavonoids, phenolic acids and aldehydes, catechin and procyanidin) in rootstocks of Crataegus laevigata (genotypes O-LE-14 and O-LE-21), Aronia melanocarpa (genotypes O-LE-14 and O-LE-21), Chaenomeles japonica (genotype O-LE-9) and Cydonia oblonga (BA 29) (genotypes O-LE-14 and O-LE-21). Hyperoside (Quercetin 3-D-galactoside) was the most abundant flavonoid compound, since its average content in the rootstocks of Crataegus laevigata (O-LE-21) was 180.68 ± 0.04 μg·g−1. Dihydrokaempherol was the least frequently found flavonoid compound, with an average concentration of 0.43 ± 0.01 μg·g−1 in all the rootstocks of plants considered in this study. Among the phenolic compounds, the most represented one was protocatechuic acid, with 955.92 ± 10.25 μg·g−1 in the rootstocks of Aronia melanocarpa (O-LE-14). On the other hand, the least represented p-Coumaric acid exhibited the average concentration of 0.34 ± 0.01 μg·g−1 in the plant rootstocks. Epicatechin was the most abundant catechin compound, with a content of 3196.37 ± 50.10 μg·g−1 in the rootstocks of Aronia melanocarpa (O-LE-14). The lowest represented catechin compound was epigallocatechin, with the average concentration of 0.95 ± 0.08 μg·g−1 in the screened plant rootstocks. From the procyanidin compounds, the most abundant one was procyanidin b2 in the rootstocks of Crataegus laevigata (O-LE-14), with a concentration of 5550.40 ± 99.56 μg·g−1. On the contrary, procyanidin a2, with an average concentration of 40.35 ± 1.61 μg·g−1, represented the least frequent procyanidin compound in all the plant rootstocks screened herein.

Author(s):  
Li-Yun Lin ◽  
Chi-Yun Huang ◽  
Kuan-Chou Chen ◽  
Robert Y. Peng

AbstractGrapefruit (G) parts contain abundant phenolic acids and flavonoids (PPNs and FLVs). The wastes created from exocarps, mesocarps (G wastes, GW) and waste blanching water (WBW) lead to a considerable loss of PPNs and FLVs. Here, we assessed Buntan (CB), Buntan Hayata (CBH), and Peiyu (CP) cultivars and observed considerable amounts of PPNs and FLVs. The ethanolic extracts of GW (GWE) and WBW contained gallic acid, protocatechuic acid, chlorogenic acid, caffeic acid, ferulic acid, coumaric acid, naringin, hesperidin, diosmin, quercetin, hesperetin, nobiletin, and tangeretin (GWC). GWE has previously been reported to be a relatively active antioxidant, anti-inflammatory (based on the expression of TNF-α in Raw264.7 cells), antihypertensive, and anti-hyperglycemic compound. The IC50 values for antihyperglycemic activity with WBW for α-amylase were as follows: CB (27.96 ± 0.03 mg/mL) < CP (28.13 ± 0.19 mg/mL) < CBH (41.60 ± 0.16 mg/mL), and those for α-glucosidase were CB (1.80 ± 0.03 mg/mL) < CP (2.97 ± 0.29 mg/mL) < CBH (9.10 ± 0.51 mg/mL). GWC upregulated Glut4 in HepG2 cells, as well as the insulin secretion capability, and intracellular levels in RIN-m5F cells, and it downregulated DPP4 in HepG2 cells. The IC50 values for the angiotensin-converting enzyme activity were: CBP (5.10 ± 0.02 mg/mL) < CB (7.24 ± 0.65 mg/mL) < CP (8.60 ± 2.03 mg/mL). Our results indicate that PPNs and FLVs present in GW are worth reclaiming.


In this paper an extensive study is reported of the very remarkable, and thus far apparently unique, case of the deformation in three dimensions of protocatechuic acid, to which attention was drawn many years ago by Otto Lehmann. The deformations are spontaneous, and are probably due to progressive gliding of the lattice planes, which exist in two configurations, one stable and the other unstable, the latter being the condition of the long prismatic rods when they first form. Such a prism presently deforms into a zigzag crystal, with stable and unstable sections in alternation which, with continuation of the deformation, becomes again straight, but now in the stable configuration. The bending is progressive, like that of an umbrella case, pendant from the end of an oblique cane pointed down, when the latter is pushed into it. The movements are so rapid that motion pictures, made with a microscope, were necessary for the observation of certain stages of the deformation. The deformations have been shown to many chemists and physicists during the past decade or more, none of whom had ever seen or heard of this remarkable type of crystal movement. The deformations are usually observed as the warm saturated solution cools, but they also occur after the crystal has been dried for many hours.


2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Fouad A. Ahmed ◽  
Rehab F. M. Ali

Brassicaspecies are very rich in health-promoting phytochemicals, including phenolic compounds, vitamin C, and minerals. The objective of this study was to investigate the effect of different blanching (i.e., water and steam) and cooking (i.e., water boiling, steam boiling, microwaving, and stir-frying) methods on the nutrient components, phytochemical contents (i.e., polyphenols, carotenoids, flavonoid, and ascorbic acid), antioxidant activity measured by DPPH assay, and phenolic profiles of white cauliflower. Results showed that water boiling and water blanching processes had a great effect on the nutrient components and caused significant losses of dry matter, protein, and mineral and phytochemical contents. However, steam treatments (blanching and cooking), stir-frying, and microwaving presented the lowest reductions. Methanolic extract of fresh cauliflower had significantly the highest antioxidant activity (68.91%) followed by the extracts of steam-blanched, steam-boiled, stir-fried, and microwaved cauliflower 61.83%, 59.15%, 58.93%, and 58.24%, respectively. HPLC analysis revealed that the predominant phenolics of raw cauliflower were protocatechuic acid (192.45), quercetin (202.4), pyrogallol (18.9), vanillic acid (11.90), coumaric acid (6.94), and kaempferol (25.91) mg/100 g DW, respectively.


2011 ◽  
Vol 56 (No. 2) ◽  
pp. 63-74 ◽  
Author(s):  
L. Pavlata ◽  
M. Chomat ◽  
A. Pechova ◽  
L. Misurova ◽  
R. Dvorak

This paper evaluates the impact of long-term supplementation of different forms of zinc (Zn) and selenium (Se) on the content of these substances in the blood and hair of goats. Two analogous supplementation experiments were performed. 37 goats divided into four groups were used in the first trial with the Zn supplementation. Group A (n = 10) was a control group (with no Zn administered). A further three groups (B, C, D) were supplemented with Zn in various forms. Group B (n = 9) with zinc oxide, Group C (n = 9) with zinc lactate and Group D (n = 9) with zinc chelate. The second trial with Se supplementation was carried out on 20 goats divided into four groups. Group E (n = 5) was a control group. The other three groups were administered Se. Group F (n = 5) was supplied with a selenium lactate-protein complex, Group G (n = 5) with sodium selenite and Group H (n = 5) with selenium yeast. Three months later blood and hair samples were taken from all animals and Zn and Se concentrations were determined in whole blood, plasma, and hair. Glutathione peroxidase (GSH-Px) activity was determined in the Se supplementation trial group. At the end of the trial the Zn concentrations in plasma and whole blood were without major differences between the groups. The plasma concentration of Zn did not increase from the initial value at the start of the trial. In hair the average concentration of Zn was 95.2&ndash;100.0 mg/kg<br />in all groups. No conclusive relation was confirmed between the values of Zn in hair and its concentration in blood. The Se concentration in whole blood (&micro;g/l) at the end of trial in supplemented groups (F &ndash; 188.8 &plusmn; 24.6; G &ndash; 197.2 &plusmn; 10.9; H &ndash; 190.1 &plusmn; 26.3) was significantly higher (P &lt; 0.01) than in the control group (E &ndash; 103.1 &plusmn; 23.5). Similarly, the activity of GSH-Px (&micro;kat/l) was significantly higher in all supplemented groups (F &ndash; 872.3 &plusmn; 94.8; G &ndash; 659.5 &plusmn; 176.4; H &ndash; 839.8 &plusmn; 150.8) than in the control group (E &ndash; 379.1 &plusmn; 63.5). Se content in hair (&micro;g/kg) was higher also in all trial groups (F &ndash; 242.3 &plusmn; 41.5; G &ndash; 200.5 &plusmn; 46.9; H &ndash; 270.0 &plusmn; 106.8) than in the control group (E &ndash; 174.7 &plusmn; 38.0). However, it was significantly (P &lt; 0.05) higher only in Group F. A conclusive correlation was identified between the Se concentration in whole blood and its content in hair (r = 0.54; P &lt; 0.05; n = 20). Based on the results it can be concluded that none of the supplemented forms of Zn increased its concentration in blood, plasma and hair. On the other hand, the administration of Se led to an increase in the Se concentration in blood, increased the activity of GSH-Px in whole blood and the Se content in hair. Based on the proven correlation and regression relation between the Se concentration in blood and its content in hair, hair can be considered as a suitable material for the diagnosis of long-term Se status in goats. Goats with sufficient Se status are those that have more than 160 &micro;g/kg of Se in hair dry weight.


1964 ◽  
Vol 19 (9) ◽  
pp. 781-783 ◽  
Author(s):  
Hans Grisebach ◽  
Karl-Otto Vollmer

Further investigations on the biosynthesis of benzoic acids in Gaultheria procumbens L. have shown that besides salicylic acid all the other benzoic acids (gentisinic acid, p-hydroxybenzoic acid, protocatechuic acid, o-pyrocatechuic acid(?), syringic acid and vanillinic acid) can be formed from cinnamic acid. In the case of vanillinic acid it was proved that the total activity is located in the carboxyl group when cinnamic acid-[3-14C] is the precursor.Formiat-14C is incorporated into the methylester group of methylsalicylate.


1964 ◽  
Vol 19 (5) ◽  
pp. 398-405 ◽  
Author(s):  
M. H. Zenk ◽  
G. Müller

Feeding experiments with glucose- (2-14C), phenylalanine- (3-14C), tyrosine- (3-14C) and p-coumaric acid- (3-14C) showed that the latter three substances are incorporated in good yields into p-hydroxybenzoic acid in leaves of Catalpa ovata. Kinetic experiments showed that p-hydroxybenzoic acid is formed from phenylalanine via p-coumaric acid and the subsequent β-oxidation of the side chain. p-Hydroxybenzoic acid can also be synthetised by hydroxylation of benzoic acid, but this does not seem to be the biosynthetic route in Catalpa.Phenylalanine- (3-14C) is also incorporated into benzoic acid, protocatechuic acid, and vanillic acid by different plants; the radioactivity of the β-C atom of the amino acid was found in each case to be located in the carboxyl group of the C6 — C1 acid. This suggests that in higher plants the benzoic acids are formed from the corresponding cinnamic acids via β-oxidation.


2020 ◽  
Vol 49 (4) ◽  
pp. 931-937
Author(s):  
Xunbing Huang ◽  
Yueyue Wang ◽  
Zehua Zhang

Abstract Erythroneura sudra is a leafhopper occurring in northern China, which causes significant damage to fruit. The relationships between E. sudra and five fruit tree species (Rosaceae) were studied for 3 yr. The highest relative density of E. sudra was recorded on leaves of Amygdalus persica L. and Cerasus pseudocerasus Lindl. Likewise, it had the highest survival rate and shortest developmental time when it fed on these two plants species, indicating that they were the most preferred by E. sudra than the other plant species. The relative density and growth performance of E. sudra were negatively correlated with the levels of tannins and flavonoids in the leaves of the host species. Both plant species had relatively lower flavonoids and tannins, and this may have contributed to the enhanced survival and population growth of E. sudra. These results can guide the development of improved management strategies for this pest.


Author(s):  
ANA KATARINA DOS SANTOS LIMA ◽  
IAN CARNEIRO DA CUNHA NÓBREGA

Parâmetros de qualidade de 10 marcas comerciais de aguardente de cana, produzidas no Estado da Paraíba, foram avaliados tendo como referenciais os padrões estabelecidos pelo Ministério da Agricultura brasileiro (graduação alcoólica, acidez volátil e cobre). Apenas duas marcas de aguardente de cana apresentaram graduação alcoólica de acordo com os valores especificados nos rótulos. Todas as marcas analisadas enquadraram-se nos padrões de acidez volátil, embora uma tenha apresentado valor relativamente elevado. A concentração de cobre na mesma amostra superou em 70% o limite tolerado pela legislação (5 mg/L), sugerindo que a alta acidez volátil contribuiu para maior arraste de cobre para o destilado. Nas demais marcas, a concentração média de cobre foi de 2,5 mg/L. Verificouse a necessidade de implantação de melhoria nos sistemas de controle internos das destilarias para que os produtos atendam os padrões estabelecidos pela legislação. ASSESSMENT OF QUALITY PARAMETERS OF BRAZILIAN SUGAR CANE SPIRITS PRODUCED IN THE STATE OF PARAIBA Abstract Quality parameters of 10 commercial brands of sugar cane spirits, produced in the state of Paraíba were evaluated using as references the established parameters by the Brazilian Ministry of Agriculture (alcoholic content, volatile acidity and copper). Only two brands of sugar cane spirits showed the alcoholic concentration in accordance to the values specified on their label. All analyzed brands were within the limits specified for volatile acidity, although one showed relatively high value. The copper concentration in the same sample exceeded 70% the level tolerated by regulations (5 mg/L), suggesting that the high volatile acidity contributed for the removal of copper for the distillate. In the other brands, the copper average concentration was of 2.5 mg/L. It was verified the need for an improvement in the internal control systems of the distilleries so that the products assist the standards established by regulations.


1959 ◽  
Vol 37 (5) ◽  
pp. 1085-1100 ◽  
Author(s):  
A. C. Neish

A number of C14-labelled compounds were fed to detached leafy twigs of Colorado spruce (Picea pungens Engelm.), and after a metabolic period of 24 hours the pungenin was isolated and the specified activities of the glucose moiety and the aglycone (3,4-dihydroxyacetophenone) were determined. In some instances the aglycone was degraded further to determine the C14 in the methyl and carbonyl carbons separately.Caffeic acid and L-phenylalanine were the best precursors of the aglycone; cinnamic acid, p-coumaric acid, phenyllactic acid, and shikimic acid were quite good. Sodium acetate was a poor precursor, and was converted to glucose more readily than to the aglycone. Compounds found to be very poor precursors include tyrosine, 3,4-dihydroxyphenylalanine, 3-hydroxytyramine, phenylacetic acid, mandelic acid, p-hydroxyphenylpyruvic acid, p-hydroxyphenyllactic acid, p-hydroxybenzoic acid, and protocatechuic acid. Cinnamic acid-α-C14 gave 3,4-dihydroxyacetophenone labelled chiefly in the methyl group, while cinnamic acid-β-C14, L-phenylalanine-β-C14, p-coumaric acid-β-C14, and caffeic acid-β-C14 formed 3,4-dihydroxyacetophenone labelled mainly in the carbonyl carbon. It appears that a phenylethanoid compound is formed by a process involving the loss of the terminal carbon of a phenylpropanoid compound.3,4-Dihydroxyacetophenone-carbonyl-C14 was fed to spruce twigs bearing new terminal growth; up to 20% was converted to pungenin but most of it formed unidentified compounds. It was a poor precursor of lignin, compared with cinnamic acid, and a poor precursor of glutamic acid, relative to acetate.


1976 ◽  
Vol 39 (1) ◽  
pp. 183-188 ◽  
Author(s):  
Leonore Loeb Adler ◽  
Pearl H. Berkowitz

The present study with 105 emotionally disturbed children was a replication of a previous “Fruit-Tree Experiment” with normal children and investigated the influence exerted by an unfamiliar story on the content of drawings. A counterbalanced design was used for two series spaced 1 yr. apart of three picture drawing sessions each. Before the second drawing session a short and novel story was read to the children. Half of the children in the experimental and control groups were simultaneously shown a pertinent picture to the story, while the other half of the children did not receive any visual aid. The results showed that the simultaneous presentation of pertinent pictures with the stories did not seem to have any effect on the responses. However, the children in this study responded very much like the normal children. The present “Fruit-Tree Experiment” showed in the graphic representations by emotionally disturbed children that changes in thought associations and imagery occurred, due to their brief exposure to an unfamiliar story.


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