scholarly journals Pechiche (Vitex cymosa Berteo ex Speng), a Nontraditional Fruit from Ecuador, is a Dietary Source of Phenolic Acids and Nutrient Minerals, in Addition to Efficiently Counteracting the Oxidative-Induced Damage in Human Dermal Fibroblasts

Antioxidants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 109
Author(s):  
Mabel Guevara ◽  
Luis A. Valdés-Silverio ◽  
María G. Granda-Albuja ◽  
Gabriel Iturralde ◽  
Tatiana Jaramillo-Vivanco ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Oxidative Damage ◽  
Phenolic Acids ◽  
Glutathione Transferase ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Atp Levels ◽  
Intracellular Ros ◽  
Phosphorus And Potassium

Pechiche fruits (Vitex cymosa Berteo ex Speng) from Ecuador were studied to determine their phenolic acid profile, nutrient minerals and capacity to protect primary human dermal fibroblasts (HDFa) against oxidative-induced damage. Up to five phenolic acids were identified, with homovanillic acid as the main one. Vitamin C, β-carotene and lutein were also determined. Phosphorus and potassium were the main macrominerals, while iron was the principal micromineral. HDFa were preincubated with a crude pechiche extract (PCext) and then subjected to oxidative stress. The activity of five antioxidant enzymes, intracellular reactive oxygen species (ROS) and ATP levels and lipid peroxidation and protein oxidation were used as markers of oxidative damage. Preincubation with PCext for 24 h allowed for the significant reduction of intracellular ROS levels, improved the intracellular ATP levels and protected lipids and proteins against oxidative damage (p < 0.05). Additionally, preincubation with PCext was also able to significantly (p < 0.05) improve the activity of the antioxidant enzymes catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase and glutathione transferase, compared to the stressed group without pretreatment. The results obtained in this study suggest the potential of pechiche as a source of bioactive compounds, as well as its beneficial effect against oxidative stress.

scholarly journals Beeswax by-Products Efficiently Counteract the Oxidative Damage Induced by an Oxidant Agent in Human Dermal Fibroblasts

2018 ◽  
Vol 19 (9) ◽  
pp. 2842 ◽  
Author(s):  
Francesca Giampieri ◽  
Massimiliano Gasparrini ◽  
Tamara Y. Forbes-Hernández ◽  
Piera Pia Manna ◽  
Jiaojiao Zhang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Oxidative Damage ◽  
Glutathione Transferase ◽  
Dermal Fibroblast ◽  
Human Dermal Fibroblast ◽  
Dermal Fibroblasts ◽  
In Vivo Studies ◽  
Thiobarbituric Acid Reactive Substance ◽  
Protective Effects ◽  
By Products

The antioxidant capacity and the phytochemical composition of two by-products from beeswax recycling processes were recently investigated. The aim of the present work was to evaluate the efficacy of one of these by-products, MUD1, against the oxidative stress induced by 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH) in human dermal fibroblast (HDF) cells. After a preliminary viability assay, the protective effect of MUD1 was investigated through the measurement of apoptosis level, the reactive oxygen species (ROS) and nitrite (NO2−) production, the level of protein and lipid biomarkers (carbonyl groups, total glutathione and thiobarbituric acid-reactive substance) of oxidative damage, and the measurement of antioxidant enzymes activities (glutatione peroxidase, glutathione reductase, glutathione transferase, superoxide dismutase and catalase). The obtained results showed that MUD1 exerted protective effects on HDF, increasing cell viability and counteracted the oxidative stress promoted by AAPH-treatment, and improved mitochondria functionality and wound healing capacities. This work shows the antioxidant effects exerted by beeswax by-products, demonstrating for the first time their potential against oxidative stress in human dermal fibroblast cells; however, further research will be necessary to evaluate their potentiality for human health by more deeply in vitro and in vivo studies.

scholarly journals Exercise protects against doxorubicin-induced oxidative stress and proteolysis in skeletal muscle

2011 ◽  
Vol 110 (4) ◽  
pp. 935-942 ◽  
Author(s):  
Ashley J. Smuder ◽  
Andreas N. Kavazis ◽  
Kisuk Min ◽  
Scott K. Powers
Keyword(s):  
Oxidative Stress ◽  
Skeletal Muscle ◽  
Antioxidant Enzymes ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Oxidative Damage ◽  
Muscle Fibers ◽  
Muscular Exercise ◽  
Protease Activation ◽  
Exercise Induced

Doxorubicin (Dox) is a potent antitumor agent used in cancer treatment. Unfortunately, Dox is myotoxic and results in significant reductions in skeletal muscle mass and function. Complete knowledge of the mechanism(s) by which Dox induces toxicity in skeletal muscle is incomplete, but it is established that Dox-induced toxicity is associated with increased generation of reactive oxygen species and oxidative damage within muscle fibers. Since muscular exercise promotes the expression of numerous cytoprotective proteins (e.g., antioxidant enzymes, heat shock protein 72), we hypothesized that muscular exercise will attenuate Dox-induced damage in exercise-trained muscle fibers. To test this postulate, Sprague-Dawley rats were randomly assigned to the following groups: sedentary, exercise, sedentary with Dox, or exercise with Dox. Our results show increased oxidative stress and activation of cellular proteases (calpain and caspase-3) in skeletal muscle of animals treated with Dox. Importantly, our findings reveal that exercise can prevent the Dox-induced oxidative damage and protease activation in the trained muscle. This exercise-induced protection against Dox-induced toxicity may be due, at least in part, to an exercise-induced increase in muscle levels of antioxidant enzymes and heat shock protein 72. Together, these novel results demonstrate that muscular exercise is a useful countermeasure that can protect skeletal muscle against Dox treatment-induced oxidative stress and protease activation in skeletal muscles.

scholarly journals Inonotus obliquus Extracts Decreased Expression of MMP1 mRNA via JNK-AP-1 Axis

Cosmetics ◽  
2020 ◽  
Vol 7 (2) ◽  
pp. 36
Author(s):  
Young Joo Kim ◽  
Hwa Jun Cha
Keyword(s):  
Oxidative Stress ◽  
Matrix Metalloproteinases ◽  
Mrna Expression ◽  
Transcriptional Activity ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Inonotus Obliquus ◽  
The Family ◽  
Normal Human ◽  
Normal Human Dermal Fibroblasts

Inonotus obliquus, which is parasitic on birch and other trees, is a fungus in the family Hymenochaetaceae. In this study, we investigated whether Inonotus obliquus extracts used in traditional medicine were decreased in the expression of matrix metalloproteinases-1 (MMP-1) in the normal human dermal fibroblasts. As shown in our results, extracts of Inonotus obliquus decreased MMP1 expression in oxidative stress-exposed normal human dermal fibroblasts. Additionally, Inonotus obliquus extracts decreased AP-1 transcriptional activity and phospho-JNK in oxidative stress-exposed normal human dermal fibroblasts. Oxidative stress mediated the elevation of MMP1 mRNA expression and was well regulated by the JNK-AP-1 axis. Therefore, the results suggest that Inonotus obliquus extracts decreased MMP1 mRNA expression by regulating JNK-AP-1 axis. Additionally, Inonotus obliquus extracts have the potential to reduce collagen destruction and the formation of wrinkles and to be used as a cosmetic ingredient.

Oxidative Stress, Mitochondrial DNA Mutation, and Impairment of Antioxidant Enzymes in Aging

2002 ◽  
Vol 227 (9) ◽  
pp. 671-682 ◽  
Author(s):  
Yau-Huei Wei ◽  
Hsin-Chen Lee
Keyword(s):  
Oxidative Stress ◽  
Mitochondrial Dna ◽  
Antioxidant Enzymes ◽  
Oxidative Damage ◽  
Large Scale ◽  
Wide Spectrum ◽  
Radical Scavenging ◽  
Mtdna Mutations ◽  
Enhanced Production ◽  
Age Related

Mitochondria do not only produce less ATP, but they also increase the production of reactive oxygen species (ROS) as byproducts of aerobic metabolism in the aging tissues of the human and animals. It is now generally accepted that aging-associated respiratory function decline can result in enhanced production of ROS in mitochondria. Moreover, the activities of free radical-scavenging enzymes are altered in the aging process. The concurrent age-related changes of these two systems result in the elevation of oxidative stress in aging tissues. Within a certain concentration range, ROS may induce stress response of the cells by altering expression of respiratory genes to uphold the energy metabolism to rescue the cell. However, beyond the threshold, ROS may cause a wide spectrum of oxidative damage to various cellular components to result in cell death or elicit apoptosis by induction of mitochondrial membrane permeability transition and release of apoptogenic factors such as cytochrome c. Moreover, oxidative damage and large-scale deletion and duplication of mitochondrial DNA (mtDNA) have been found to increase with age in various tissues of the human. Mitochondria act like a biosensor of oxidative stress and they enable cell to undergo changes in aging and age-related diseases. On the other hand, it has recently been demonstrated that impairment in mitochondrial respiration and oxidative phosphorylation elicits an increase in oxidative stress and causes a host of mtDNA rearrangements and deletions. Here, we review work done in the past few years to support our view that oxidative stress and oxidative damage are a result of concurrent accumulation of mtDNA mutations and defective antioxidant enzymes in human aging.

Guava (Psidium guajava L. cv. Red Suprema) Crude Extract Protect Human Dermal Fibroblasts against Cytotoxic Damage Mediated by Oxidative Stress

2018 ◽  
Vol 73 (1) ◽  
pp. 18-24 ◽  
Author(s):  
José M. Alvarez-Suarez ◽  
Francesca Giampieri ◽  
Massimiliano Gasparrini ◽  
Luca Mazzoni ◽  
Tamara Y. Forbes-Hernández ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Crude Extract ◽  
Psidium Guajava ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts

scholarly journals The protective effect of acerola (Malpighia emarginata) against oxidative damage in human dermal fibroblasts through the improvement of antioxidant enzyme activity and mitochondrial functionality

2017 ◽  
Vol 8 (9) ◽  
pp. 3250-3258 ◽  
Author(s):  
José M. Alvarez-Suarez ◽  
Francesca Giampieri ◽  
Massimiliano Gasparrini ◽  
Luca Mazzoni ◽  
Celestino Santos-Buelga ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Oxidative Damage ◽  
Protective Effect ◽  
Antioxidant Enzyme ◽  
Bioactive Compounds ◽  
Antioxidant Enzyme Activity ◽  
Dermal Fibroblasts ◽  
Natural Source ◽  
Human Dermal Fibroblasts ◽  
Malpighia Emarginata

Acerola fruits are shown as an important natural source of bioactive compounds with beneficial properties for health.

Retinol, at concentrations greater than the physiological limit, induces oxidative stress and apoptosis in human dermal fibroblasts

2004 ◽  
Vol 13 (1) ◽  
pp. 45-54 ◽  
Author(s):  
Amparo Gimeno ◽  
Rosa Zaragoza ◽  
Inma Vivo-Sese ◽  
Juan R. Vina ◽  
Vicente J. Miralles
Keyword(s):  
Oxidative Stress ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Physiological Limit
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