scholarly journals Light-Controlled Fruit Pigmentation and Flavor Volatiles in Tomato and Bell Pepper

Antioxidants ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 14
Author(s):  
Hee Ju Yoo ◽  
Jin-Hyun Kim ◽  
Kyoung-Sub Park ◽  
Jung Eek Son ◽  
Je Min Lee
Keyword(s):  
Gene Expression ◽  
Molecular Mechanisms ◽  
Chalcone Synthase ◽  
Bell Pepper ◽  
Carotenoid Content ◽  
Future Research ◽  
Plant Family ◽  
Total Carotenoid Content ◽  
Pepper Fruit ◽  
Ripe Tomato

Light is a major environmental factor affecting the regulation of secondary metabolites, such as pigments and flavor. The Solanaceae plant family has diverse patterns of fruit metabolisms that serve as suitable models to understand the molecular basis of its regulation across species. To investigate light-dependent regulation for fruit pigmentation and volatile flavors, major fruit pigments, their biosynthetic gene expression, and volatiles were analyzed in covered fruits of tomato and bell pepper. Immature covered fruits were found to be ivory in color and no chlorophyll was detected in both plants. The total carotenoid content was found to be reduced in ripe tomato and bell pepper under cover. Naringenin chalcone decreased more than 7-fold in ripe tomato and total flavonoids decreased about 10-fold in immature and ripe pepper fruit under light deficiency. Light positively impacts fruit pigmentation in tomato and bell pepper by regulating gene expression in carotenoid and flavonoid biosynthesis, especially phytoene synthase and chalcone synthase, respectively. Nineteen volatile flavors were detected, and seven of these exhibited light-dependent regulations for both ripe tomato and pepper. This study will help in improving fruit quality and aid future research works to understand the molecular mechanisms regulating the influence of light-dependency on pigments and flavor volatiles.

Evaluation of Appropriate Reference Genes For Investigating Gene Expression in Chlorops oryzae (Diptera: Chloropidae)

2019 ◽  
Vol 112 (5) ◽  
pp. 2207-2214 ◽  
Author(s):  
Ping Tian ◽  
Lin Qiu ◽  
Ailin Zhou ◽  
Guo Chen ◽  
Hualiang He ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ribosomal Protein ◽  
Reference Genes ◽  
Molecular Mechanisms ◽  
Developmental Stages ◽  
Quantitative Polymerase Chain Reaction ◽  
Housekeeping Genes ◽  
Future Research ◽  
Economic Damage ◽  
Physiological Processes

Abstract Reverse transcription quantitative polymerase chain reaction (PCR) has become an invaluable technique for analyzing gene expression in many insects. However, this approach requires the use of stable reference genes to normalize the data. Chlorops oryzae causes significant economic damage to rice crops throughout Asia. The lack of suitable reference genes has hindered research on the molecular mechanisms underlying many physiological processes of this species. In this study, we used quantitative real-time PCR to evaluate the expression of eight C. oryzae housekeeping genes glyceraldehyde-3-phosphate dehydrogenase (GAPDH), β-actin (βACT), beta-tubulin (βTUB), Delta Elongation factor-1 (EF1δ), ribosomal protein S11 (RPS11), RPS15, C-terminal-Binding Protein (CtBP), and ribosomal protein 49 (RP49) in different developmental stages and tissues in both larvae and adults. We analyzed the data with four different software packages: geNorm, NormFinder, BestKeeper, and RefFinder and compared the results obtained with each method. The results indicate that PRS15 and RP49 can be used as stable reference genes for quantifying gene expression in different developmental stages and larval tissues. GAPDH and βACT, which have been considered stable reference genes by previous studies, were the least stable of the candidate genes with respect to larval tissues. GAPDH was, however, the most stable reference gene for adult tissues. We verified the candidate reference genes identified and found that the expression levels of Cadherins (Cads) changed when different reference genes were used to normalize gene expression. This study provides a valuable foundation for future research on gene function, and investigating the molecular basis of physiological processes, in C. oryzae.

scholarly journals MAP Kinase Pathways in the YeastSaccharomyces cerevisiae

1998 ◽  
Vol 62 (4) ◽  
pp. 1264-1300 ◽  
Author(s):  
Michael C. Gustin ◽  
Jacobus Albertyn ◽  
Matthew Alexander ◽  
Kenneth Davenport
Keyword(s):  
Gene Expression ◽  
Signaling Pathways ◽  
Molecular Mechanisms ◽  
Current Knowledge ◽  
Regulation Of Gene Expression ◽  
Mapk Cascade ◽  
Mitogen Activated Protein Kinase ◽  
Future Research ◽  
Mapk Pathways ◽  
Mapk Cascades

SUMMARY A cascade of three protein kinases known as a mitogen-activated protein kinase (MAPK) cascade is commonly found as part of the signaling pathways in eukaryotic cells. Almost two decades of genetic and biochemical experimentation plus the recently completed DNA sequence of the Saccharomyces cerevisiae genome have revealed just five functionally distinct MAPK cascades in this yeast. Sexual conjugation, cell growth, and adaptation to stress, for example, all require MAPK-mediated cellular responses. A primary function of these cascades appears to be the regulation of gene expression in response to extracellular signals or as part of specific developmental processes. In addition, the MAPK cascades often appear to regulate the cell cycle and vice versa. Despite the success of the gene hunter era in revealing these pathways, there are still many significant gaps in our knowledge of the molecular mechanisms for activation of these cascades and how the cascades regulate cell function. For example, comparison of different yeast signaling pathways reveals a surprising variety of different types of upstream signaling proteins that function to activate a MAPK cascade, yet how the upstream proteins actually activate the cascade remains unclear. We also know that the yeast MAPK pathways regulate each other and interact with other signaling pathways to produce a coordinated pattern of gene expression, but the molecular mechanisms of this cross talk are poorly understood. This review is therefore an attempt to present the current knowledge of MAPK pathways in yeast and some directions for future research in this area.

scholarly journals Molecular Characterization, Expression Analysis of Carotenoid, Xanthophyll, Apocarotenoid Pathway Genes, and Carotenoid and Xanthophyll Accumulation in Chelidonium majus L.

Plants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1753
Author(s):  
Ramaraj Sathasivam ◽  
Hyeon Ji Yeo ◽  
Chang Ha Park ◽  
Minsol Choi ◽  
Haejin Kwon ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Higher Plants ◽  
Carotenoid Biosynthesis ◽  
Carotenoid Content ◽  
Binding Motif ◽  
Herbaceous Plant ◽  
Biosynthesis Pathway ◽  
Chelidonium Majus ◽  
Perennial Herbaceous Plant ◽  
Total Carotenoid Content

Chelidonium majus L. is a perennial herbaceous plant that has various medicinal properties. However, the genomic information about its carotenoid biosynthesis pathway (CBP), xanthophyll biosynthesis pathway (XBP), and apocarotenoid biosynthesis pathway (ABP) genes were limited. Thus, the CBP, XBP, and ABP genes of C. majus were identified and analyzed. Among the 15 carotenoid pathway genes identified, 11 full and 4 partial open reading frames were determined. Phylogenetic analysis of these gene sequences showed higher similarity with higher plants. Through 3D structural analysis and multiple alignments, several distinct conserved motifs were identified, including dinucleotide binding motif, carotene binding motif, and aspartate or glutamate residues. Quantitative RT-PCR showed that CBP, XBP, and ABP genes were expressed in a tissue-specific manner; the highest expression levels were achieved in flowers, followed by those in leaves, roots, and stems. The HPLC analysis of the different organs showed the presence of eight different carotenoids. The highest total carotenoid content was found in leaves, followed by that in flowers, stems, and roots. This study provides information on the molecular mechanisms involved in CBP, XBP, and ABP genes, which might help optimize the carotenoid production in C. majus. The results could also be a basis of further studies on the molecular genetics and functional analysis of CBP, XBP, and ABP genes.

scholarly journals Sexual dimorphism and sex-biased gene expression in an egg parasitoid species, Anastatus disparis

2019 ◽  
Author(s):  
P.C. Liu ◽  
DeJun Hao ◽  
Hao-Yuan Hu ◽  
Jian-Rong Wei
Keyword(s):  
Gene Expression ◽  
Molecular Mechanisms ◽  
Parasitoid Wasp ◽  
Egg Parasitoid ◽  
Future Research ◽  
Biological Traits ◽  
Sexually Dimorphic ◽  
Functional Categories ◽  
Expression Of Genes ◽  
Go Enrichment

Abstract Background Differences in the expression of genes present in both sexes are assumed to contribute to sex differences including behavioural, physiological and morphological dimorphisms. For enriching our knowledge of gender differences in an important egg parasitoid wasp, Anastatus disparis (Hymenoptera: Eupelmidae), sex-biased differences in gene expression were investigated using Illumina-based transcriptomic analysis. Results A total of 67201 resulting unigenes were annotated, including 4206 genes differentially expressed (i.e., sex-biased genes) between females and males including 243 specific genes; the majority of the sex-biased genes (63.72%) were female-biased. Sexually dimorphic traits related to flyability and longevity were discussed at the level of gene expression, improving our understanding of those biological traits. Besides, Gene Ontology (GO) enrichment analyses showed that the functional categories in sex-biased genes were mainly related to reproduction. Conclusions Overall, this study provides comprehensive insight into the sexually dimorphic traits of parasitoid wasps, offering a basis for future research to better understand the molecular mechanisms underlying such traits and improve the application of these insects to the biological control of pests.

scholarly journals Effects of Conventional and Bokashi Hydroponics on Vegetative Growth, Yield and Quality Attributes of Bell Peppers

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1281
Author(s):  
René Clarisse Tong ◽  
Charles Stephen Whitehead ◽  
Olaniyi Amos Fawole
Keyword(s):  
Vegetative Growth ◽  
Vegetation Index ◽  
Growth Parameters ◽  
Bell Pepper ◽  
Plant Performance ◽  
Postharvest Quality ◽  
Carotenoid Content ◽  
Future Research ◽  
Pepper Plant ◽  
Hydroponic Systems

Due to consumers’ awareness and concern about nutrition and health in different parts of the world, the adoption of organic hydroponics is increasing. This has led to a search for organic nutrient media. One of the viable nutrient sources for organic hydroponics is bokashi compost. The principal objective of this study was to compare the performance of 10% bokashi hydroponics with convention hydroponics for bell pepper production. The different hydroponics influenced vegetative growth parameters largely due to considerable differences in the mineral elements in both hydroponic systems. Stems of conventionally grown plants were significantly (p ≤ 0.05) thicker (10.2 mm) compared to those of the bokashi grown plants (7.3 mm). Conventionally grown plants had significantly (p ≤ 0.05) higher photosynthetic performance than bokashi grown plants; normalized difference vegetation index (NDVI) (78.80 versus 67.49), soil plant analysis development (SPAD; 73.89 versus 38.43), and quantum yield (QY; 0.64 versus 0.49). Leaf superoxide dismutase (SOD) activity in the leaves of bokashi grown plants (0.32 units/mg protein) was significantly (p ≤ 0.05) lower than in the leaves of conventionally grown plants (0.37 units/mg protein). This also corresponded to significantly (p ≤ 0.05) higher leaf sap content in the conventionally grown plant than bokashi grown plants. Furthermore, conventional hydroponics yielded three-fold greater pepper fruit per plant compared to bokashi. After 14 days of storage at 7 °C and 95% relative humidity, the firmness of both groups declined, especially for the bokashi grown fruit (27.73 shore unit), which was significantly lower compared to conventionally grown fruit (35.65 shore unit). However, there was an increase in carotenoid content in fruit grown in both hydroponic systems after storage. In conclusion, although bell pepper plant was successfully cultivated in bokashi hydroponics, the plant performance, fruit yield and postharvest quality were lower than conventional hydroponics. We believe that this study and its approach will provide future research with baseline information on optimizing media of bokashi hydroponics to produce bell pepper.

scholarly journals Sexual dimorphism and sex-biased gene expression in an egg parasitoid species, Anastatus disparis

2020 ◽  
Author(s):  
P.C. Liu ◽  
DeJun Hao ◽  
Hao-Yuan Hu ◽  
Jian-Rong Wei
Keyword(s):  
Gene Expression ◽  
Molecular Mechanisms ◽  
Parasitoid Wasp ◽  
Egg Parasitoid ◽  
Future Research ◽  
Sexually Dimorphic ◽  
Large Set ◽  
Pest Species ◽  
Expression Of Genes ◽  
Go Enrichment

Abstract BackgroundDifferences in the expression of genes present in both sexes are assumed to contribute to sex differences including behavioural, physiological and morphological dimorphisms. For enriching our knowledge of gender differences in an important egg parasitoid wasp, Anastatus disparis (Hymenoptera: Eupelmidae), sex-biased differences in gene expression were investigated using Illumina-based transcriptomic analysis. ResultsA total of 15812 resulting unigenes were annotated, and a large set of genes accounting for 50.09% of the total showed sex-biased expression and included 630 sex-specific genes. Gene Ontology (GO) enrichment analyses showed that the functional categories associated with sex-biased genes were mainly related to reproduction. In addition, the transcriptome data provided evidence that sex pheromones in A. disparis are produced by the female, and activity of 12-desaturases appear to have been replaced by 9-desaturases playing roles in sex pheromone production. The large set of sex-biased genes identified in this study provide a molecular background for sexually dimorphic traits such as flyability, longevity, and aggression in this species and suggests candidate venom proteins expressed only in females that could be used for biological control.ConclusionsThis study provides comprehensive insight into sexually dimorphic traits of a parasitoid wasp and can inform future research into the molecular mechanisms underlying such traits and the application of parasitoids to the biological control of pest species.

scholarly journals Expression of a Chromoplast-Specific Lycopene β-Cyclase Gene (CYC-B) Is Implicated in Carotenoid Accumulation and Coloration in the Loquat

Biomolecules ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 874 ◽  
Author(s):  
Min Hong ◽  
Zhuo-Heng Chi ◽  
Yong-Qing Wang ◽  
Yue-Ming Tang ◽  
Qun-Xian Deng ◽  
...  
Keyword(s):  
Gene Expression ◽  
Metabolic Pathway ◽  
Negative Control ◽  
Carotenoid Content ◽  
Virus Induced Gene Silencing ◽  
Carotenoid Accumulation ◽  
Homologous Genes ◽  
Total Carotenoid Content ◽  
The Difference ◽  
Β Carotene

Carotenoids are the principal pigments in the loquat. Although the metabolic pathway of plant carotenoids has been extensively investigated, few studies have been explored the regulatory mechanisms of loquat carotenoids because knowledge of the loquat genome is incomplete. The chromoplast-specific lycopene β-cyclase gene (CYC-B) could catalyze cyclization of lycopene to β-carotene. In this study, the differential accumulation patterns of loquat with different colors were analyzed and virus-induced gene silencing (VIGS) was utilized in order to verify CYC-B gene function. Using a cloning strategy of homologous genes, a CYC-B gene orthologue was successfully identified from the loquat. At a later stage of maturation, CYC-B gene expression and carotenoids concentrations in the ‘Dawuxing’ variety were higher than in ‘Chuannong 1-5-9′, possibly leading to the difference in pulp coloration of loquat. Interference of CYC-B gene expression in the loquat demonstrated clear visual changes. The green color in negative control fruits became yellow, while TRV2-CYC-B silenced fruits remained green. CYC-B gene expression and total carotenoid content in the pulp decreased by 32.5% and 44.1%, respectively. Furthermore, multiple key genes in the carotenoid metabolic pathway synergistically responded to downregulation of CYC-B gene expression. In summary, we provide direct evidences that CYC-B gene is involved in carotenoid accumulation and coloration in the loquat.

scholarly journals Glucosinolates, Carotenoids, and Vitamins E and K Variation from Selected Kale and Collard Cultivars

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Moo Jung Kim ◽  
Yu-Chun Chiu ◽  
Kang-Mo Ku
Keyword(s):  
Nutritional Value ◽  
Total Content ◽  
Carotenoid Content ◽  
Mature Stage ◽  
Future Research ◽  
Total Carotenoid ◽  
Total Carotenoid Content ◽  
Cultivar Selection ◽  
Significant Difference ◽  
Fat Soluble Vitamins

Glucosinolates, carotenoids, and fat-soluble vitamins E and K contents were analyzed from various kale and collard cultivars at mature stage. We found a significant difference in these phytonutrients among cultivars. Among kale cultivars, “Beira” and “Olympic Red” were the highest in the total glucosinolate and “Toscano” kale was the highest in total carotenoid content. “Scarlet” kale was highest in tocopherols. For collard, total glucosinolate was the highest in “Top Bunch” while carotenoids were the highest in “Green Glaze.” An accession PI261597 was the highest in phylloquinone. In addition to the total content of each phytonutrient class, their composition differed among cultivars, indicating that each cultivar may have differential regulatory mechanisms for biosynthesis of these phytonutrients. Our result indicates that cultivar selection may play an important role in consumption of kale and collard with greater nutritional benefit. Therefore, the result of this study will provide a more thorough profile of essential and nonessential phytonutrients of kale and collard cultivars for consumers’ choice and for future research on nutritional value of these crops.

scholarly journals Whole Blood Transcriptome Sequencing Reveals Gene Expression Differences between Dapulian and Landrace Piglets

2016 ◽  
Vol 2016 ◽  
pp. 1-10
Author(s):  
Jiaqing Hu ◽  
Dandan Yang ◽  
Wei Chen ◽  
Chuanhao Li ◽  
Yandong Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Whole Blood ◽  
Molecular Mechanisms ◽  
Future Research ◽  
Nucleotide Polymorphisms ◽  
Rna Seq ◽  
Single Nucleotide ◽  
Neonatal Stage ◽  
Pathway Analyses ◽  
Blood Transcriptome

There is little genomic information regarding gene expression differences at the whole blood transcriptome level of different pig breeds at the neonatal stage. To solve this, we characterized differentially expressed genes (DEGs) in the whole blood of Dapulian (DPL) and Landrace piglets using RNA-seq (RNA-sequencing) technology. In this study, 83 DEGs were identified between the two breeds. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses identified immune response and metabolism as the most commonly enriched terms and pathways in the DEGs. Genes related to immunity and lipid metabolism were more highly expressed in the DPL piglets, while genes related to body growth were more highly expressed in the Landrace piglets. Additionally, the DPL piglets had twofold more single nucleotide polymorphisms (SNPs) and alternative splicing (AS) than the Landrace piglets. These results expand our knowledge of the genes transcribed in the piglet whole blood of two breeds and provide a basis for future research of the molecular mechanisms underlying the piglet differences.

scholarly journals Muscle transcriptome provides the first insight into the dynamics of gene expression with progression of age in sheep

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Reena Arora ◽  
Naveen Kumar Siddaraju ◽  
S. S. Manjunatha ◽  
S. Sudarshan ◽  
Mohamed Nadeem Fairoze ◽  
...  
Keyword(s):  
Gene Expression ◽  
Metal Binding ◽  
Skeletal Muscles ◽  
Molecular Mechanisms ◽  
Muscle Development ◽  
Muscle Protein ◽  
Age Groups ◽  
Future Research ◽  
Sequencing Data ◽  
Insight Into

AbstractThe dynamic synergy of genes and pathways in muscles in relation to age affects the muscle characteristics. Investigating the temporal changes in gene expression will help illustrate the molecular mechanisms underlying muscle development. Here we report the gene expression changes in skeletal muscles through successive age groups in Bandur, a meat type sheep of India. RNA sequencing data was generated from the longissimus thoracis muscles from four age groups, ranging from lamb to adult. Analysis of 20 highest expressed genes common across the groups revealed muscle protein, phosphorylation, acetylation, metal binding and transport as significant functions. Maximum differentiation was observed after 2.5–3 years on transition from lambs to adult. Transcriptional regulation by the TFAP2 transcription factors, IL-6 signaling and PI3K/AKT signaling pathways were enriched in younger animals. The gene-protein network demarcated key interactive genes involved in muscle development and proliferation that can be used as candidates for future research on improvement of muscle characteristics.

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