scholarly journals Specific Activity of Superoxide Dismutase in Stallion Seminal Plasma Is Related to Sperm Cryotolerance

Antioxidants ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 539 ◽  
Author(s):  
Marion Papas ◽  
Jaime Catalán ◽  
Beatriz Fernandez-Fuertes ◽  
Laura Arroyo ◽  
Anna Bassols ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Seminal Plasma ◽  
Specific Activity ◽  
Routine Practice ◽  
Oxygen Species ◽  
Sperm Cryopreservation ◽  
Before And After ◽  
Specific Activities ◽  
Plasma Antioxidant

While the removal of seminal plasma is a routine practice prior to equine sperm cryopreservation, this fluid contains the main source of antioxidant enzymes able to scavenge these reactive oxygen species. Therefore, stallion seminal plasma components may have an impact on ejaculate freezability. Against this background, this study was designed to investigate whether the activities of the main stallion seminal plasma antioxidant enzymes are related to sperm cryotolerance. With this purpose, 16 ejaculates were collected from 14 healthy stallions, and each ejaculate was split into two aliquots. The first one was used to evaluate the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), and glutathione reductase (GSR) in seminal plasma. The second aliquot was extended and then processed for cryopreservation. Sperm motility and viability were evaluated before and after cryopreservation, and ejaculates were classified as of good (GFE) or poor freezability (PFE) based on total motile and viable spermatozoa at post-thaw. We observed that, while the specific activities of CAT, GPX, and GSR were similar between GFE and PFE, that of SOD was significantly (p < 0.05) higher in GFE than in PFE. We can thus conclude that, in stallions, the specific activity of SOD in the seminal plasma of a given ejaculate might be related to its freezability.

Assessment of Superoxide Dismutase and Catalase Evolution in Different Stages of an Experimental Endometriosis

2017 ◽  
Vol 68 (6) ◽  
pp. 1381-1383
Author(s):  
Allia Sindilar ◽  
Carmen Lacramioara Zamfir ◽  
Eusebiu Viorel Sindilar ◽  
Alin Constantin Pinzariu ◽  
Eduard Crauciuc ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Reproductive Function ◽  
Female Rats ◽  
Oxygen Species ◽  
Efficient Treatment ◽  
Endometrial Cells ◽  
Gynecological Disorder ◽  
The Impact

Endometriosis is described as a gynecological disorder characterized by the presence of endometrial tissue outside the uterus; extensively explored because of its increasing incidency, with an indubitable diagnostic only after invasive surgery, with no efficient treatment, it has still many aspects to be elucidated. A growing body of facts sustain oxidative stress as a crucial factor between the numerous incriminated factors implicated in endometriosis ethiopathogeny. Reactive oxygen species(ROS) act to decline reproductive function. Our study intends to determine if an experimental model of endometriosis may be useful to assess the impact of oxidative stress on endometrial cells; we have used a murine model of 18 adult Wistar female rats. A fragment from their left uterine horn was implanted in the abdominal wall. After 4 weeks, a laparatomy was performed, 5 endometrial implants were removed, followed by biochemical tissue assay of superoxide dismutase(SOD) and catalase(CAT). At the end of the experiment, the rats were sacrificed, the implants were removed for histopathological exam and biochemical assay of antioxidant enzymes. The results revealed decreased levels of antioxidant enzymes, pointing on significant oxidative stress involvement.

scholarly journals Semen quality, lipid peroxidation, and seminal plasma antioxidant status in horses with different intensities of physical exercise

2013 ◽  
Vol 82 (1) ◽  
pp. 31-35 ◽  
Author(s):  
Helena Härtlová ◽  
Radko Rajmon ◽  
Iva Krontorádová ◽  
Jiří Mamica ◽  
Lukáš Zita ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Seminal Plasma ◽  
Aspartate Aminotransferase ◽  
Semen Quality ◽  
Antioxidant Activities ◽  
Membrane Damage ◽  
Antioxidant Enzyme Activities ◽  
Reference Ranges ◽  
Stress Symptoms ◽  
Plasma Antioxidant

The aim of this study was to compare markers of semen quality, sperm membrane damage, and the seminal plasma antioxidant activity in warmblood stallions with and without sport workload stress. Four stallions were used for breeding only (control) and four both for breeding and competition in jumping. Semen samples were collected at 14-day intervals (from June to August) from each stallion (5 ejaculates per stallion). Immediately after sperm collection, a conventional examination of the ejaculate was processed. Catalytic activities of enzymes aspartate aminotransferase, alanin aminotransferase, glutathione peroxidase, superoxide dismutase and indicator of lipoperoxidation - F2α isoprostanes were measured in samples of seminal plasma. Contrary to basic semen quality indicators, the values of seminal plasma pH, aspartate aminotransferase and alanin aminotransferase were significantly (P < 0.05) impaired in the physically stressed stallions. Also, the level of F2α isoprostanes and the activity of superoxide dismutase were significantly (P < 0.05) increased by stress. The antioxidant activities of superoxide dismutase and glutathion peroxidase increased during the monitored period and reflected changes in F2α isoprostane concentration. We can conclude that even the conventional basic sperm indicators stay within the reference ranges of the biochemical indicators of seminal plasma such as pH or AST/ALT activity may be negatively influenced by sport workload stress. Increased concentrations of F2α isoprostanes indicate that lipoperoxidation can be a mechanism of cell membrane destabilization, which is counteracted by an increase of antioxidant enzyme activities. This is the first report of oxidative stress symptoms in normospermic equine semen in relation to stallion sport workload.

scholarly journals Thermal dependence of the antioxidant enzymes superoxide dismutase, catalase, and peroxidase in foliage of Iris pumila L.

2009 ◽  
Vol 61 (3) ◽  
pp. 441-446 ◽  
Author(s):  
Ana Vuleta ◽  
Branka Tucic
Keyword(s):  
Activation Energy ◽  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Temperature Increase ◽  
Specific Activity ◽  
Thermal Dependence ◽  
Full Sunlight ◽  
Iris Pumila ◽  
Temperature Optima

Thermal dependence of the enzymes SOD, CAT, and POD was investigated in leaves of Iris pumila plants inhabiting two contrasting light environments, a sun-exposed dune site and a woodland understory. At the same assay temperature, both the specific activity and the activation energy of SOD and CAT were higher in plants inhabiting vegetation shade than in those experiencing full sunlight. Conversely, the temperature optima for the two enzymes did not differ between alternative radiation environments. The specific activity of POD increased with temperature increase, and was always greater in plants growing under full sunlight than in those from vegetation shade. The activation energy of POD was higher than that of SOD or CAT, being lower in sun-than in shade-exposed plants.

scholarly journals Expression of oxidative stress in metastatic retinoblastomaa comparative study

2012 ◽  
Vol 4 (2) ◽  
pp. 271-276 ◽  
Author(s):  
S Mukhopadhyay ◽  
J Dutta ◽  
R Raut ◽  
H Datta ◽  
A K Bhattacharyay
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Distant Metastasis ◽  
Venous Blood ◽  
Oxygen Species ◽  
Blood Samples ◽  
Number Of Patients ◽  
Intraocular Retinoblastoma

Objective: To compare oxidative stress between primary retinoblastoma and retinoblastoma with distant metastasis. Patients and methods: Forty consecutive patients presented with primary retinoblastoma and the same number of patients presented with distant metastasis, attending the outpatient department of our hospital between August 2002 and April 2005. All the patients with retinoblastoma underwent a standard metastasis workup and were subsequently categorized into two groups (without metastasis and with metastasis).Venous blood samples were drawn from each patient. After proper centrifugation, serum was collected and antioxidant enzymes and reactive oxygen species (ROS) were assayed. Main outcome measures: Serum collected from the patients was subjected to biochemical assay of the antioxidant enzymes (superoxide dismutase, catalase and peroxidise) and ROS to determine any difference in enzyme activity between the two groups. Results: Antioxidant levels were found to be less in the metastasis group as compared to the primary intraocular retinoblastoma group(p<0.05).Mean ROS activity was found to be increased in metastatic group (p<0.05). Conclusion: The decreased antioxidant enzymes level along with increased ROS activity in patients with metastatic retinoblastoma reflect increased oxidative stress as compared to primary intraocular retinoblastoma patients.DOI: http://dx.doi.org/10.3126/nepjoph.v4i2.6543 Nepal J Ophthalmol 2012; 4 (2): 271-276  

scholarly journals The Effect of Sperm Preparation Medium on Malondialdehyde Concentration in the Seminal Plasma of Normozoospermic and Oligoastheno- teratozoospermic patient

2009 ◽  
Vol 3 (2) ◽  
pp. 74-79
Author(s):  
Maha Khalid Abd-Al Kareem
Keyword(s):  
Seminal Plasma ◽  
The Other ◽  
Oxygen Species ◽  
Sperm Preparation ◽  
Fertilization Rates ◽  
Oxidative Damages ◽  
Infertile Men ◽  
Acrosomal Reaction ◽  
Before And After ◽  
Intra Uterine Insemination

Intra uterine insemination (IUI) has been widely used to treat infertility. Infertility may be due to increased levels of reactive oxygen species (ROS) in the seminal plasma. The goal of our study was to confirm that using sperm preparation medium to activate the spermatozoa in IUI might be helpful to decrease the levels of ROS in the seminal plasma, lowering the oxidative stress and the damage effects of the sperm and enhance a good quality sperm capable of capacitation, acrosomal reaction and fertilization. The levels of malondialdehyde (MDA) were measured in the seminal plasma in 40 infertile men of normozoospermia and oligoastheno-teratozoospermia before and after adding medium to the samples. Results showed that there was a significant (P<0.05) decrease in all parameters of semen samples in oligoastheno-teratozoosperia as compared with the other group, there was no significant increase in the level of MDA before adding the medium to the samples and a significant (P<0.05) increase after adding the medium to the same samples in the second group as compared with normozoospermic patients. Results reported in this study, clearly indicated that adding sperm preparation medium to the semen samples would improve the fertilization rates by decreasing the oxidative damages to the spermatozoa. Our results might be need a further studies to find a clue for the treatment of infertility in man.

scholarly journals Age-Related Changes in Hepatic Activity and Expression of Detoxification Enzymes in Male Rats

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Erika Vyskočilová ◽  
Barbora Szotáková ◽  
Lenka Skálová ◽  
Hana Bártíková ◽  
Jitka Hlaváčová ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Specific Activity ◽  
Detoxification Enzymes ◽  
Male Rats ◽  
Cellular Functions ◽  
Metabolizing Enzymes ◽  
Age Related ◽  
Male Wistar Rats ◽  
Old Rats ◽  
Specific Activities

Process of aging is accompanied by changes in the biotransformation of xenobiotics and impairment of normal cellular functions by free radicals. Therefore, this study was designed to determine age-related differences in the activities and/or expressions of selected drug-metabolizing and antioxidant enzymes in young and old rats. Specific activities of 8 drug-metabolizing enzymes and 4 antioxidant enzymes were assessed in hepatic subcellular fractions of 6-week-old and 21-month-old male Wistar rats. Protein expressions of carbonyl reductase 1 (CBR1) and glutathioneS-transferase (GST) were determined using immunoblotting. Remarkable age-related decrease in specific activities of CYP2B, CYP3A, and UDP-glucuronosyl transferase was observed, whereas no changes in activities of CYP1A2, flavine monooxygenase, aldo-keto reductase 1C, and antioxidant enzymes with advancing age were found. On the other hand, specific activity of CBR1 and GST was 2.4 folds and 5.6 folds higher in the senescent rats compared with the young ones, respectively. Interindividual variability in CBR1 activity increased significantly with rising age. We suppose that elevated activities of GST and CBR1 may protect senescent rats against xenobiotic as well as eobiotic electrophiles and reactive carbonyls, but they may alter metabolism of drugs, which are CBR1 and especially GSTs substrates.

scholarly journals Timing the evolution of antioxidant enzymes in cyanobacteria

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Joanne S. Boden ◽  
Kurt O. Konhauser ◽  
Leslie J. Robbins ◽  
Patricia Sánchez-Baracaldo
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Free Radicals ◽  
Molecular Oxygen ◽  
Molecular Clocks ◽  
Oxygen Species ◽  
Terrestrial Habitats ◽  
Iron Nickel

AbstractThe ancestors of cyanobacteria generated Earth’s first biogenic molecular oxygen, but how they dealt with oxidative stress remains unconstrained. Here we investigate when superoxide dismutase enzymes (SODs) capable of removing superoxide free radicals evolved and estimate when Cyanobacteria originated. Our Bayesian molecular clocks, calibrated with microfossils, predict that stem Cyanobacteria arose 3300–3600 million years ago. Shortly afterwards, we find phylogenetic evidence that ancestral cyanobacteria used SODs with copper and zinc cofactors (CuZnSOD) during the Archaean. By the Paleoproterozoic, they became genetically capable of using iron, nickel, and manganese as cofactors (FeSOD, NiSOD, and MnSOD respectively). The evolution of NiSOD is particularly intriguing because it corresponds with cyanobacteria’s invasion of the open ocean. Our analyses of metalloenzymes dealing with reactive oxygen species (ROS) now demonstrate that marine geochemical records alone may not predict patterns of metal usage by phototrophs from freshwater and terrestrial habitats.

Salt stress in a halophilic bacterium: alterations in oxidative metabolism and oxy-intermediate scavenging systems

1994 ◽  
Vol 40 (12) ◽  
pp. 1057-1063 ◽  
Author(s):  
Nancy J. Brown-Peterson ◽  
Marvin L. Salin
Keyword(s):  
Superoxide Dismutase ◽  
Salt Stress ◽  
Exponential Growth ◽  
Specific Activity ◽  
Fold Increase ◽  
Halophilic Bacterium ◽  
Growth Media ◽  
Metabolic Rates ◽  
Specific Activities ◽  
Scavenging Enzymes

Halobaeterium halobium, an archaebacterium that grows optimally in 4 M NaCl, was subjected to hypo-osmotic stress by growth in media containing either 1, 1.25, 1.65, or 2 M NaCl. Exponential growth occurred over a 48-h period in 2 M NaCl as well as in the standard 4 M NaCl growth medium. In growth media containing NaCl in a concentration below 2 M, exponential growth was observed after a 6-h lag. After 12 h of growth in the lowest NaCl concentrations, extracts from H. halobium showed nearly a 100-fold increase in the specific activity of catalase compared with bacteria grown at higher NaCl concentrations; superoxide dismutase activity increased 10-fold, whereas peroxidase activity increased 4- to 5-fold compared with the activities at the higher salt levels. Specific activities remained elevated throughout the 48-h period. In contrast, malate dehydrogenase activity was not affected by alterations in the salt environment. Respiration of H. halobium growing in the most hyposaline medium was significantly elevated at 12 h and remained high over the ensuing 36 h. Metabolic rates correlated with increases observed in oxy-intermediate scavenging enzymes. No additional isozymes of superoxide dismutase were induced by salt stress. However, the catalase activity emanated from a newly induced mesohalic catalase distinct from the constitutive catalase–peroxidase.Key words: Halobaeterium halobium, metabolism, oxy-intermediate, superoxide dismutase, catalase.

Mitigation of oxidant injury to lung microvasculature by intratracheal instillation of antioxidant enzymes

1993 ◽  
Vol 265 (4) ◽  
pp. L340-L345 ◽  
Author(s):  
M. L. Barnard ◽  
R. R. Baker ◽  
S. Matalon
Keyword(s):  
Antioxidant Enzymes ◽  
Antioxidant Enzyme ◽  
Intratracheal Instillation ◽  
Antioxidant Enzyme Activities ◽  
Oxygen Species ◽  
Oxidant Injury ◽  
Lung Weight ◽  
Control Value ◽  
Before And After ◽  
Baseline Values

We quantitated the ability of intratracheally administered liposome-encapsulated antioxidant enzymes to reduce reactive oxygen species injury to the pulmonary microvasculature. Cationic liposomes containing 3,500 U of Cu,Zn superoxide dismutase (Cu,Zn SOD) and 3,124 U of catalase were instilled into rabbits. The animals were killed 2-72 h later and their lungs were removed and perfused with Krebs Ringer with 5% wt/vol of fat-free bovine serum albumin. The pulmonary filtration co-efficient (Kf,c) was measured before and after adding 500 microM xanthine and 5 mU/ml xanthine oxidase (XO) into the lung perfusate. Two hours after a single intratracheal instillation of liposome-entrapped Cu,Zn SOD and catalase, lung antioxidant enzyme activities were 34 and 125% higher than the corresponding control values, remained virtually unchanged for up to 8 h post-instillation, and then decreased, reaching baseline values between 24 and 72 h. Addition of xanthine and XO into the lung perfusate of un-instilled rabbits, or rabbits that received liposomes with inactivated enzymes, caused a 100% increase in Kf,c (control value: 2 +/- 0.12 ml.min-1 x cmH2O-1 per 100 g dry lung weight). On the other hand, Kf,c values of rabbits lungs instilled with liposome-encapsulated active Cu,Zn SOD and catalase and challenged with xanthine and XO 8-24 h later remained at baseline levels. Instillation of liposomes containing either enzyme was equally effective in preventing the increase in Kf,c, indicating that both superoxide anions and hydrogen peroxide were necessary for the initiation of injury. We concluded that intratracheal instillation of liposome-encapsulated antioxidant enzymes caused a transient increase of lung antioxidant enzyme levels which protects the pulmonary microvasculature from free radical-initiated injury.

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