scholarly journals Analysis of Oxidative Stress-Related Markers in Crohn’s Disease Patients at Surgery and Correlations with Clinical Findings

Antioxidants ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 378 ◽  
Author(s):  
Cristina Luceri ◽  
Elisabetta Bigagli ◽  
Sara Agostiniani ◽  
Francesco Giudici ◽  
Daniela Zambonin ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Oxidation ◽  
Disease Activity ◽  
Severe Disease ◽  
Oxidation Products ◽  
Therapeutic Monitoring ◽  
Thiobarbituric Acid Reactive Substance ◽  
Reducing Ability ◽  
Lipid Oxidation Products ◽  
End Products

Crohn’ disease (CD) patients are at high risk of postoperative recurrence and new tools for the assessment of disease activity are needed to prevent long-term complications. In these patients, the over-production of ROS generated by inflamed bowel tissue and inflammatory cells activates a pathogenic cascade that further exacerbates inflammation and leads to increased oxidative damage to DNA, proteins, and lipids. We measured the products of protein/lipid oxidation and the total antioxidant capacity (ferric reducing ability of plasma, FRAP) in the serum of CD patients with severe disease activity requiring surgery with the aim to characterize their redox status and identify associations between oxidative stress-related markers and their clinical characteristics. At the systemic level, CD was associated with increased levels of protein and lipid oxidation products when compared to healthy volunteers, even though the FRAP values were similar. Advanced oxidation protein product (AOPP) levels showed the highest difference between patients and the controls (11.25, 5.02–15.15, vs. 1.36, 0.75–2.70, median, interquartile range; p < 0.0001) and the analysis of receiver operating characteristic (ROC) curves, indicated for AOPP, the best area under the curve (AUC) value for CD prediction. Advanced glycated end-products (AGEs) were also significantly higher in CD patients (p < 0.01), which is of interest since AOPP and AGEs are both able to activate the membrane receptor for advanced glycation end products (RAGE) involved in inflammatory diseases. Thiobarbituric acid reactive substance (TBARS) levels were significantly higher in CD patients with ileal localization and aggressive disease behavior, in smokers, and in patients suffering from allergies. In conclusion, our data indicate that circulating oxidative stress biomarkers may be attractive candidates as disease predictors as well as for clinical or therapeutic monitoring of CD. Our results also suggest that AOPP/AGEs and RAGE signaling may represent a pathogenic factor and a potential therapeutic target in CD.

scholarly journals Modification of proteins by reactive lipid oxidation products and biochemical effects of lipoxidation

2019 ◽  
Vol 64 (1) ◽  
pp. 19-31 ◽  
Author(s):  
Corinne M. Spickett ◽  
Andrew R. Pitt
Keyword(s):  
Lipid Oxidation ◽  
Conformational Changes ◽  
Protein Interactions ◽  
Structural Changes ◽  
Cell Metabolism ◽  
Cellular Level ◽  
Oxidation Products ◽  
Beneficial Effects ◽  
Lipid Oxidation Products ◽  
End Products

Abstract Lipid oxidation results in the formation of many reactive products, such as small aldehydes, substituted alkenals, and cyclopentenone prostaglandins, which are all able to form covalent adducts with nucleophilic residues of proteins. This process is called lipoxidation, and the resulting adducts are called advanced lipoxidation end products (ALEs), by analogy with the formation of advanced glycoxidation end products from oxidized sugars. Modification of proteins by reactive oxidized lipids leads to structural changes such as increased β-sheet conformation, which tends to result in amyloid-like structures and oligomerization, or unfolding and aggregation. Reaction with catalytic cysteines is often responsible for the loss of enzymatic activity in lipoxidized proteins, although inhibition may also occur through conformational changes at more distant sites affecting substrate binding or regulation. On the other hand, a few proteins are activated by lipoxidation-induced oligomerization or interactions, leading to increased downstream signalling. At the cellular level, it is clear that some proteins are much more susceptible to lipoxidation than others. ALEs affect cell metabolism, protein–protein interactions, protein turnover via the proteasome, and cell viability. Evidence is building that they play roles in both physiological and pathological situations, and inhibiting ALE formation can have beneficial effects.

scholarly journals PSIV-5 Can dietary oxidized protein induce oxidative stress in pigs?

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 181-181
Author(s):  
Carl A Frame ◽  
Logan R Kilburn ◽  
Erika M Johnson ◽  
Mariana C Rossoni Serao
Keyword(s):  
Oxidative Stress ◽  
Companion Animals ◽  
Thiobarbituric Acid ◽  
Oxidation Products ◽  
Protein Carbonyls ◽  
P Value ◽  
Thiobarbituric Acid Reactive Substance ◽  
Oxidation Treatment ◽  
Crypt Depth ◽  
Lipid Oxidation Products

Abstract Endogenous protein oxidation as a result of oxidative stress is known to reduce the efficiency of livestock species (Boler et al., 2012; DeRouchey et al., 2004; Dibner, Atwell, Kitchell, Shermer, & Ivey, 1996). Additionally, rendered by-products are common feedstuffs in livestock diets. During processing, these sources have the potential to become oxidized. While most research on oxidative stress has focused on consumption of dietary oxidized lipids, little research has been done in the area of dietary oxidized proteins and the potential to induce oxidative stress. The objective of this study was to determine the effects of dietary oxidized protein on oxidative stress in pigs. For this study, 30 pigs 6 weeks old were divided into three dietary treatments of control, medium, and high dietary oxidized protein. Each treatment was fed the same diet, with the exception of the degree of oxidation in bovine plasma which was included in the diet at 10 percent. Pigs were fed for 19 days and then euthanized for tissues collected. Jejunum, liver, and colon were collected along with urine and plasma samples on day 0 and 18. Jejunum samples were also collected for histology. Markers of oxidative stress included protein carbonyls, thiobarbituric acid reactive substance (TBARS), 8-hydroxyguanine, and glutathione peroxidase activity. Pigs in the high oxidation treatment had an increase in crypt depth of 16 percent (p-value less than 0.05) when compared to control further resulting in an 11 percent decrease in villi height to crypt depth ratio (p-value less than 0.05). Additionally, lipid oxidation products, measured by TBARS, was 28 percent greater in the liver of pigs in the medium oxidation treatment (p-value less than 0.05) when compared to control. Even with the short duration of this study, dietary oxidized protein did impact the oxidative status of the animal. Using pigs as a model for companion animals, it could be hypothesized then that long-term exposure could have implications on longevity.

Differential PDGF secretion by graft and aortic SMC in response to oxidized LDL

2002 ◽  
Vol 283 (2) ◽  
pp. H725-H732 ◽  
Author(s):  
Afaf Absood ◽  
Akira Furutani ◽  
Tsutomu Kawamura ◽  
Linda M. Graham
Keyword(s):  
Oxidative Stress ◽  
Lipid Oxidation ◽  
Oxidized Ldl ◽  
Low Density Lipoprotein ◽  
Vascular Grafts ◽  
Density Lipoprotein ◽  
Oxidation Products ◽  
Enzyme Linked Immunosorbent Assay ◽  
Oxidized Low Density Lipoprotein ◽  
Lipid Oxidation Products

Smooth muscle cells (SMC) from prosthetic vascular grafts constitutively secrete higher levels of platelet-derived growth factor-AA (PDGF-AA) than aortic SMC. Lipid oxidation products accumulate in grafts and may stimulate PDGF production. The effect of oxidized low-density lipoprotein (oxLDL) on PDGF-AA secretion by aortic and graft SMC was compared. SMC isolated from canine thoracic aorta or Dacron thoracoabdominal grafts ( n = 10) were incubated with native LDL or oxLDL (0–400 μg/ml) for 72 h. PDGF-AA in the conditioned medium was measured with enzyme-linked immunosorbent assay. OxLDL increased PDGF-AA production by graft SMC from 78 ± 2 to 256 ± 16 pg PDGF/μg DNA and aortic SMC from 21 ± 1 to 40 ± 2 pg PDGF/μg DNA. Native LDL had no effect. N-acetylcysteine inhibited oxLDL-induced PDGF increase. Both superoxide and H2O2 stimulated PDGF secretion by graft SMC had little effect on aortic SMC. Our results suggest that PDGF production by graft (synthetic) SMC is more sensitive to stimulation by oxidative stress than aortic (contractile) SMC. Lipid oxidation products that accumulate in prosthetic vascular grafts can cause an oxidative stress, which stimulates PDGF production by graft SMC. PDGF can induce migration of aortic SMC onto the graft, contributing to the development of intimal hyperplasia.

Asymmetric desorption of lipid oxidation products induces membrane bending

Soft Matter ◽  
2021 ◽  
Author(s):  
Rui Jin ◽  
Tobias Baumgart
Keyword(s):  
Oxidative Stress ◽  
Lipid Oxidation ◽  
Cellular Membrane ◽  
Oxidation Products ◽  
Metabolic Processes ◽  
Lipid Oxidation Products ◽  
Membrane Bending

Lipid oxidation, detected in metabolic processes, is induced in excess when the cellular membrane suffers extra oxidative stress. Lipid oxidation can compromise biomembrane function in part through perturbations of lipid...

scholarly journals Accumulation of Aluminium and Physiological Status of Tree Foliage in the Vicinity of a Large Aluminium Smelter

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
E. D. Wannaz ◽  
J. H. Rodriguez ◽  
T. Wolfsberger ◽  
H. A. Carreras ◽  
M. L. Pignata ◽  
...  
Keyword(s):  
Lipid Oxidation ◽  
Membrane Lipid ◽  
Physiological Parameters ◽  
Oxidation Products ◽  
Physiological Status ◽  
Pollution Gradient ◽  
Sulphur Deposition ◽  
Aluminium Production ◽  
Lipid Oxidation Products ◽  
Tree Foliage

A pollution gradient was observed in tree foliage sampled in the vicinity of a large aluminium production facility in Patagonia (Argentina). Leaves ofEucalyptus rostrata, and Populus hybridusand different needle ages ofPinusspec. were collected and concentrations of aluminium (Al) and sulphur (S) as well as physiological parameters (chlorophyll and lipid oxidation products) were analyzed. Al and S concentrations indicate a steep pollution gradient in the study showing a relationship with the physiological parameters in particular membrane lipid oxidation products. The present study confirms that aluminium smelting results in high Al and sulphur deposition in the study area, and therefore further studies should be carried out taking into account potentially adverse effects of these compounds on human and ecosystem health.

Volatile Lipid Oxidation Products of Wagyu and Domestic Breeds of Beef

1996 ◽  
Vol 44 (4) ◽  
pp. 1091-1095 ◽  
Author(s):  
Terri D. Boylston ◽  
Sarah A. Morgan ◽  
Kristen A. Johnson ◽  
Ray W. Wright, ◽  
Jan R. Busboom ◽  
...  
Keyword(s):  
Lipid Oxidation ◽  
Oxidation Products ◽  
Lipid Oxidation Products
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