scholarly journals Qualitative Chemical Characterization and Multidirectional Biological Investigation of Leaves and Bark Extracts of Anogeissus leiocarpus (DC.) Guill. & Perr. (Combretaceae)

Antioxidants ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 343 ◽  
Author(s):  
Orlando ◽  
Ferrante ◽  
Zengin ◽  
Sinan ◽  
Bene ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Cell Line ◽  
Hct116 Cell ◽  
Stem Bark ◽  
Rat Colon ◽  
Cholinesterase Inhibition ◽  
Ionization Mass ◽  
Hct116 Cell Line ◽  
Anogeissus Leiocarpus ◽  
Ethyl Acetate Extracts

Anogeissus leiocarpus (DC.) Guill. & Perr. (Combretaceae) has a long history of use by folk populations for the management of multiple human ailments. Based on the published literature, there has been no attempt to conduct a comparative assessment of the biological activity and the phytochemical profiles of the leaves and stem bark of A. leiocarpus extracted using methanol, ethyl acetate, and water. By high-performance liquid chromatography with electrospray ionization mass spectrometric detection (HPLC-ESI-MSn) analysis, quinic, shikimic, gallic, and protocatechuic acids were tentatively identified from all the extracts, while chlorogenic, caffeic, ferulic, and dodecanedioic acids were only characterised from the leaves extracts. Additionally, a pharmacological study was carried out to evaluate potential protective effects that are induced by the extracts in rat colon and colon cancer HCT116 cell line. In general, the methanol and water extracts of A. leiocarpus leaves and stem bark showed potent radical scavenging and reducing properties. It was noted that the stem bark extracts were more potent antioxidants as compared to the leaves extracts. The methanol extract of A. leiocarpus leaves showed the highest acetyl (4.68 mg galantamine equivalent/g) and butyryl (4.0 mg galantamine equivalent/g) cholinesterase inhibition. Among ethyl acetate extracts, the pharmacological investigation suggested stem bark ethyl acetate extracts to be the most promising. This extract revealed ability to protect rat colon from lipopolysaccharide-induced oxidative stress, without exerting promoting effects on HCT116 cell line viability and migration. As a conclusion, A. leiocarpus represents a potential source of bioactive compounds in the development of novel therapeutic agents.

scholarly journals Regulation of Transforming Growth Factor α Expression in a Growth Factor-Independent Cell Line

1998 ◽  
Vol 18 (1) ◽  
pp. 303-313 ◽  
Author(s):  
Gillian M. Howell ◽  
Lisa E. Humphrey ◽  
Barry L. Ziober ◽  
Rana Awwad ◽  
Basker Periyasamy ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cell Line ◽  
Antisense Rna ◽  
Promoter Activity ◽  
Transforming Growth Factor ◽  
Hct116 Cell ◽  
Malignant Phenotype ◽  
Hct116 Cell Line ◽  
Transforming Growth Factor Α ◽  
Factor Α

ABSTRACT Aberrant transcriptional regulation of transforming growth factor α (TGFα) appears to be an important contributor to the malignant phenotype and the growth factor independence with which malignancy is frequently associated. However, little is known about the molecular mechanisms responsible for dysregulation of TGFα expression in the malignant phenotype. In this paper, we report on TGFα promoter regulation in the highly malignant growth factor-independent cell line HCT116. The HCT116 cell line expresses TGFα and the epidermal growth factor receptor (EGFR) but is not growth inhibited by antibodies to EGFR or TGFα. However, constitutive expression of TGFα antisense RNA in the HCT116 cell line resulted in the isolation of clones with markedly reduced TGFα mRNA and which were dependent on exogenous growth factors for proliferation. We hypothesized that if TGFα autocrine activation is the major stimulator of TGFα expression in this cell line, TGFα promoter activity should be reduced in the antisense TGFα clones in the absence of exogenous growth factor. This was the case. Moreover, transcriptional activation of the TGFα promoter was restored in an antisense-TGFα-mRNA-expressing clone which had reverted to a growth factor-independent phenotype. Using this model system, we were able to identify a 25-bp element within the TGFα promoter which conferred TGFα autoregulation to the TGFα promoter in the HCT116 cell line. In the TGFα-antisense-RNA-expressing clones, this element was activated by exogenous EGF. This 25-bp sequence contained no consensus sequences of known transcription factors so that the TGFα or EGF regulatory element within this 25-bp sequence represents a unique element. Further characterization of this 25-bp DNA sequence by deletion analysis revealed that regulation of TGFα promoter activity by this sequence is complex, as both repressors and activators bind in this region, but the overall expression of the activators is pivotal in determining the level of response to EGF or TGFα stimulation. The specific nuclear proteins binding to this region are also regulated in an autocrine-TGFα-dependent fashion and by exogenous EGF in EGF-deprived TGFα antisense clone 33. This regulation is identical to that seen in the growth factor-dependent cell line FET, which requires exogenous EGF for optimal growth. Moreover, the time response of the stimulation oftrans-acting factor binding by EGF suggests that the effect is directly due to growth factor and not mediated by changes in growth state. We conclude that this element appears to represent the major positive regulator of TGFα expression in the growth factor-independent HCT116 cell line and may represent the major site of transcriptional dysregulation of TGFα promoter activity in the growth factor-independent phenotype.

Assessment of the Anticancer and Antigenotoxic potential of Costus speciosus extract against HCT116 cell line

2017 ◽  
Vol 29 (1) ◽  
Author(s):  
Eman Althubaiti ◽  
Abdulkader Shaikh Omar ◽  
Mohammed Almatry ◽  
Yasir Anwar ◽  
Soufyan ElAssouli
Keyword(s):  
Cell Line ◽  
Hct116 Cell ◽  
Hct116 Cell Line ◽  
Costus Speciosus

scholarly journals Isolation and Purification of Bioactive Compounds from the Stem Bark of Jatropha podagrica

Molecules ◽  
2019 ◽  
Vol 24 (5) ◽  
pp. 889 ◽  
Author(s):  
Truong Minh ◽  
Tran Xuan ◽  
Hoang-Dung Tran ◽  
Truong Van ◽  
Yusuf Andriana ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Ethyl Acetate ◽  
Gallic Acid ◽  
Bioactive Compounds ◽  
Stem Bark ◽  
Ionization Mass ◽  
Methyl Gallate ◽  
Antioxidant Power ◽  
Isolation And Purification ◽  
Chemical Structures

This paper reports the successive isolation and purification of bioactive compounds from the stem bark of Jatropha podagrica, a widely known medicinal plant. The ethyl acetate extract of the stem bark exhibited the strongest antioxidant activity assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging, and ferric reducing antioxidant power (FRAP) assays (IC50 = 46.7, 66.0, and 492.6, respectively). By column chromatography (CC) with elution of hexane and ethyl acetate at 8:2, 7:3, and 6:4 ratios, the isolation of this active extract yielded five fractions (C1–C5). Chemical structures of the constituents included in C1–C5 were elucidated by gas chromatography-mass spectrometry (GC-MS), electrospray ionization-mass spectrometry (ESI-MS), and nuclear magnetic resonance (NMR) and resolved as methyl gallate (C1, C2, C3, C4), gallic acid (C1, C2), fraxetin (C2, C3, C4, C5), and tomentin (C3). Mixture C2 (IC50 DPPH and ABTS = 2.5 µg/mL) and C3 (IC50 FRAP = 381 µg/mL) showed the highest antioxidant properties. Among the isolated fractions, C4 was the most potential agent in growth inhibition of six bacterial strains including Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Listeria monocytogenes, Bacillus subtilis, and Proteus mirabilis (MIC = 5, 20, 30, 20, 25, and 20 mg/mL, respectively). All identified constituents exerted an inhibitory activity on the growth of Lactuca sativa, of which the mixture C3 performed the maximal inhibition on shoot (IC50 = 49.4 µg/mL) and root (IC50 = 47.1 µg/mL) growth. Findings of this study suggest that gallic acid, methyl gallate, fraxetin, and tomentin isolated from J. podagrica possessed antioxidant, antibacterial, and growth inhibitory potentials.

scholarly journals Pre-treated theaflavin-3,3′-digallate has a higher inhibitory effect on the HCT116 cell line

2017 ◽  
Vol 61 (1) ◽  
pp. 1400340 ◽  
Author(s):  
Yangping Ding ◽  
Bingcan Chen ◽  
Zili Gao ◽  
Huayi Suo ◽  
Hang Xiao
Keyword(s):  
Cell Line ◽  
Hct116 Cell ◽  
Inhibitory Effect ◽  
Hct116 Cell Line

scholarly journals In Silico Docking of Phaleria Macrocarpa Bark Compounds Through Inflammation Pathway and its Cytotoxic Activities Against HCT116 Cell Line

2019 ◽  
Vol 35 (1) ◽  
pp. 471-475
Author(s):  
Aryo Tedjo ◽  
Fadilah Fadilah ◽  
Kusmardi Kusmardi ◽  
Rafika Indah Paramita ◽  
Fadhilah Harmen ◽  
...  
Keyword(s):  
Cell Line ◽  
In Silico ◽  
Hct116 Cell ◽  
Cytotoxic Activities ◽  
Hct116 Cell Line ◽  
In Silico Docking ◽  
Phaleria Macrocarpa

scholarly journals Circulating microRNA-194 and microRNA-1228 Could Predict Colon Cancer Proliferation via Phospho S6 Modulation

2020 ◽  
Vol 29 (3) ◽  
pp. 361-367
Author(s):  
Sergiu Pasca ◽  
Calin Ionescu ◽  
David Andras ◽  
Dan Eniu ◽  
Mihai Andrei Muresan ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cell Line ◽  
Western Blotting ◽  
Hct116 Cell ◽  
Cell Counting ◽  
Circulating Microrna ◽  
Cancer Proliferation ◽  
Hct116 Cell Line ◽  
Qrt Pcr ◽  
Advanced Colon Cancer

Background and Aims: Although colon cancer has a decreasing incidence trend in Europe, because of its still high frequency and not fully understood pathogenesis, this malignancy still remains a subject of intense research. The aim of this study was to investigate the role of microRNA-194 and microRNA-1228 in colon cancer proliferation. Methods: RNA was extracted from patients with colon cancer with or without advanced disease and microRNA expression levels were determined through qRT-PCR. Assays were performed on HCT116 cell line and included qRT-PCR, western blotting and cell counting. Results: We observed that both microRNAs 194 and 1228 were altered in patients with colon cancer compared with healthy individuals. We observed a lower expression of both microRNA-194 and microRNA-1228 in patients with advanced colon cancer. To validate their pathogenetic role we performed viability and invasion assays on HCT116 cell line transfected with mimics or inhibitors of the mentioned microRNAs, with observable changes in viability and invasion. Furthermore, to determine the altered signaling induced by these microRNAs, we performed western blotting for phospho S6 on HCT116 cells transfected with mimic and inhibitor of the above-mentioned microRNAs with observable differences. Conclusion: In the current study we have shown that both microRNA-194 and microRNA-1228 alteration was correlated with the presence of advanced colon cancer, a fact that was further validated in vitro through an invasion assay. Moreover, we have also shown that their effect might be mediated through phospho S6 expression.

scholarly journals Comparative Study of Phytochemical, Antioxidant, and Cytotoxic Activities and Phenolic Content of Syzygium aqueum (Burm. f. Alston f.) Extracts Growing in West Sumatera Indonesia

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Afrizal Itam ◽  
Mutia Siska Wati ◽  
Vina Agustin ◽  
Nursal Sabri ◽  
Rafika Aris Jumanah ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Stem Bark ◽  
Natural Antioxidant ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Hexane Extracts ◽  
Phytochemical Constituents ◽  
Ethyl Acetate Extracts

Syzygium aqueum, consisting of various fruit colors, is one of the plants that have been used as traditional medicine. This study aims to evaluate and compare phytochemical, antioxidant, and cytotoxic activities and total phenolic content of leaves and stem bark extracts of S. aqueum with pink and red fruits, in order to identify the best extract that can be used as a natural antioxidant. Phytochemical constituents were evaluated qualitatively using chemicals, while cytotoxic activities were identified using the brine shrimp lethality test. Total phenolic content was determined via the Folin–Ciocalteu method. Leaves and stem bark of S. aqueum contained flavonoids, phenolics, and triterpenoids, but the stem bark also contained saponins and alkaloids. Methanol and ethyl acetate extracts of leaves and stem bark were categorized as very powerful antioxidants to DPPH (IC50 9.71–38.69 μg/mL) and hydrogen peroxide (IC50 16.44–44.02 μg/mL), while hexane extracts were inactive. Methanol, ethyl acetate, and hexane extracts of leaves and stem bark were categorized as moderately cytotoxic to A. salina larvae (LC50 104.04–440.65 μg/mL). Comparing leaves and stem barks, antioxidant and cytotoxic activities of stem bark extracts were higher than those of leaves extracts. Total phenolic content of leaves extracts was higher than that of stem bark extracts where the order of total phenolic content progressed from methanol extracts > ethyl acetate extracts > hexane extracts. Therefore, the stem bark of S. aqueum was identified as the better source of natural antioxidant compared with the leaves.

scholarly journals Comparison of the Qualitative Chemical Composition of Extracts from Ageratina havanensis Collected in Two Different Phenological Stages by FIA-ESI-IT-MSn and UPLC/ESI-MSn: Antiviral Activity

2017 ◽  
Vol 12 (1) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Trina H. García ◽  
Claudia Quintino da Rocha ◽  
Marcelo J. Dias ◽  
Liudis L. Pino ◽  
Gloria del Barrio ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Chemical Composition ◽  
Antiviral Activity ◽  
Ethyl Acetate ◽  
High Performance ◽  
Ethyl Acetate Extract ◽  
Reproductive Season ◽  
Ionization Mass ◽  
Herpes Simplex Viruses ◽  
Ethyl Acetate Extracts

The flowers and leaves of Ageratina havanensis (Kunth) R. M. King & H. Robinson are traditionally used as a tea to cure several diseases. The production of active secondary metabolites can be affected by several environmental factors such as climate, altitude, rainfall, phenological stage and other conditions that may influence the growth of plants. In this sense, the development of a methodology to compare the chemical composition of plant extracts is needed. The qualitative chemical composition of the ethyl acetate extracts of flowers and leaves, collected in both reproductive and non-reproductive season, was determined by flow injection analysis-electrospray ionization-ion trap tandem mass spectrometry (FIA-ESI-IT-MSn) and ultra-high-performance liquid chromatography coupled to electrospray negative ionization mass spectrometry (UPLC/ESI-MSn). The qualitative chemical composition of the ethyl acetate extracts of flowers and leaves was very similar in all cases. Also the antiviral activity of flowers against human herpes simplex viruses type 1 and 2 (HSV-1, HSV-2) ( Herpesviridae) was analyzed. Three glucoside flavonoids were isolated from the ethyl acetate extract of the leaves of A. havanensis collected in flowering season using chromatographic methods and their structures were elucidated by physical and spectroscopic data measurements, and by comparing the obtained data with previously published values. The compounds were identified as 3- O-β-D-glucosyl-7-methoxyaromadendrin (5), 7- O-β-D-glucosyl-4′-dihydroxy-5-methoxyflavanone (6) and 5- O-β-D-glucosylsakuranetin (7); this is the first report of the isolation of these compounds in the Asteraceae family. Since the qualitative composition of the extracts of A. havanensis was similar in all cases, it can be expected that the ethyl acetate extract of the leaves collected in the non-reproductive season has anti-herpetic activity similar to that obtained in the reproductive season.

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