scholarly journals Comparative Study of Phytochemical, Antioxidant, and Cytotoxic Activities and Phenolic Content of Syzygium aqueum (Burm. f. Alston f.) Extracts Growing in West Sumatera Indonesia

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Afrizal Itam ◽  
Mutia Siska Wati ◽  
Vina Agustin ◽  
Nursal Sabri ◽  
Rafika Aris Jumanah ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Stem Bark ◽  
Natural Antioxidant ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Hexane Extracts ◽  
Phytochemical Constituents ◽  
Ethyl Acetate Extracts

Syzygium aqueum, consisting of various fruit colors, is one of the plants that have been used as traditional medicine. This study aims to evaluate and compare phytochemical, antioxidant, and cytotoxic activities and total phenolic content of leaves and stem bark extracts of S. aqueum with pink and red fruits, in order to identify the best extract that can be used as a natural antioxidant. Phytochemical constituents were evaluated qualitatively using chemicals, while cytotoxic activities were identified using the brine shrimp lethality test. Total phenolic content was determined via the Folin–Ciocalteu method. Leaves and stem bark of S. aqueum contained flavonoids, phenolics, and triterpenoids, but the stem bark also contained saponins and alkaloids. Methanol and ethyl acetate extracts of leaves and stem bark were categorized as very powerful antioxidants to DPPH (IC50 9.71–38.69 μg/mL) and hydrogen peroxide (IC50 16.44–44.02 μg/mL), while hexane extracts were inactive. Methanol, ethyl acetate, and hexane extracts of leaves and stem bark were categorized as moderately cytotoxic to A. salina larvae (LC50 104.04–440.65 μg/mL). Comparing leaves and stem barks, antioxidant and cytotoxic activities of stem bark extracts were higher than those of leaves extracts. Total phenolic content of leaves extracts was higher than that of stem bark extracts where the order of total phenolic content progressed from methanol extracts > ethyl acetate extracts > hexane extracts. Therefore, the stem bark of S. aqueum was identified as the better source of natural antioxidant compared with the leaves.

Phytochemical and antioxidant activity of Cadaba farinosa Forssk stem bark extracts

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Emmanuel M. Halilu ◽  
Abdullahi M. Abdurrahman ◽  
Sylvester N. Mathias ◽  
Chinenye J. Ugwah-Oguejiofor ◽  
Muntaka Abdulrahman ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Stem Bark ◽  
Free Radical Scavenging ◽  
Total Phenolic

Abstract Cadaba farinosa is used in traditional medicine for treatment of cancer, diabetes, and rheumatism. The research was aimed at evaluating the phytochemical and antioxidant activity of the extracts. The powdered stem bark was extracted successively with the aid of Soxhlet extractor using n-hexane, ethyl acetate, and methanol. The resulting extracts were concentrated on rotary evaporator and the percentage yields were calculated. The phytochemical and TLC profiles of the extracts were studied. The antioxidant activity of the extracts and ascorbic acid (standard) were determined using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical assay. The total phenolic content of the extracts and tannic acid (standard) were evaluated using Folin–Ciocalteu reagent. The percentage yields of n-hexane, ethyl acetate, and methanol extracts were found to be 1.19, 1.37, and 13.93%, respectively. The phytochemical screening revealed the presence of tannins, flavonoids, saponins, cardiac glycosides, alkaloids, and triterpenoids. The TLC profiles of the extracts revealed the presence of compounds as evidenced from their R f values. The total phenolic content of ethyl acetate and methanol extracts were found to be 135 and 112 mg, respectively. The free radical scavenging activity demonstrated by the extracts was comparable to ascorbic acid. The ethyl acetate extract had higher phenolic content and demonstrated the highest free radical scavenging with IC50 31.07 mg/mL. The results of research have provided strong preliminary evidence of antioxidant activity which may be because of phenolic compounds in the extracts.

Antioxidant activity of extracts from the bark of Chamaecyparis lawsoniana (A. Murray) Parl.

Holzforschung ◽  
2006 ◽  
Vol 60 (4) ◽  
pp. 459-462 ◽  
Author(s):  
Heng Gao ◽  
Todd F. Shupe ◽  
Chung Y. Hse ◽  
Thomas L. Eberhardt
Keyword(s):  
Ethyl Acetate ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Hexane Extracts ◽  
Chamaecyparis Lawsoniana

Abstract The bark of Chamaecyparis lawsoniana (A. Murray) Parl. was extracted with methanol and sequentially partitioned with n-hexane, ethyl acetate, n-butanol and deionized water. The antioxidant activities of the four extracts were evaluated using the DPPH• and ABTS+• methods. The total phenolic content of the extracts was determined using the Folin-Ciocalteu method and expressed as gallic acid equivalents (GAE). Butylated hydroxytoluene was used as a positive control in the radical-scavenging activity tests. All the bark extracts showed significant radical-scavenging activity. In the ABTS+• assay, n-butanol extracts exhibited the strongest radical-scavenging activity, followed by ethyl acetate, water, and n-hexane extracts. The greatest total phenolic content was 428.54 mg GAE per gram of dry extract and was detected in the n-butanol extract, followed by the ethyl acetate and n-hexane extracts. The antioxidant activities correlate with the amount of phenolics present in these extracts. The ethyl acetate and n-butanol extracts were rich in phenolics and may represent a good source of antioxidants.

scholarly journals Kandungan Total Fenolat pada Ekstrak Rhizophora sp Dari Teluk Awur, Jepara

2019 ◽  
Vol 22 (1) ◽  
pp. 27
Author(s):  
Ali Ridlo ◽  
Endang Supriyantini ◽  
Sri Sedjati
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethyl Acetate Extract ◽  
Stem Bark ◽  
Total Phenolic ◽  
Plant Parts ◽  
Ic50 Values ◽  
Gallic Acid Equivalent

The antioxidant activity and total phenolic content of 18 extracts from different plant parts (leaves and stem bark) of 3 mangroves R. mucronata,  R. apiculata andR. stylosa from Teluk Awur, Jeparawere examined. The content of total phenolics in the extracts was calculated as gallic acid equivalent (GAE) and antioxidant activity was estimated as IC50 values using 1,1-diphenyl-2-picrylhydrazyl (DPPH). The samples were extracted with n-hexane, ethyl acetate, and methanol respectively. The result showed that ethyl acetate extract of stem bark of R. apiculata holded the highest antioxidant activity with IC50 47.52ppm. Rates of total phenolic content ranged from 5.15 ± 1.02 to 64.79 ± 2.75 mg GAE/g.There was a significant correlation between total phenolic content and antioxidant activity. The results indicated promising mangrove Rhizophora sp. for the utilization as significant source of natural antioxidant. Telah dilakukan uji kandungan total fenolat dan aktivitas antioksidan 18 ekstrak dari bagian tanaman (daun dan kulit batang) 3 spesies mangrove asal Teluk Awur Jepara, yaitu mangroves R. mucronata,  R. apiculatadan R. stylosa. Kandungan total fenolat ditentukan sebagai ekuivalen asam galat (GAE) dan aktivitas antioksidan ditentukan berdasarInhibition Concentration 50 (IC50) dengan menggunakan radikal bebas DPPH (1,1-diphenyl-2-picrylhydrazyl). Sampel diekstraksi secara bertngkat menggunakan n-heksana, etil asetat dan metanol. Hasil penelitian menunjukkan ekstrak etil asetat kulit kayu mangrove R. apiculata memiliki aktivitas antioksidan terbaik dengan IC5047.52ppm. Nilai kandungan total fenolat ekstrak berkisar antara 5.15 ± 1.02 sampai  64.79 ± 2.75 mg GAE/g. Terdapat korelasi kuat antara kandungan total fenolat dengan aktivitas antioksidan. Hasil ini menunjukkan bahwa mangrove Rhizophora sp. dapat dijadikan sebagai sumber senyawa antioksidan alami.

scholarly journals Antioxidant, Cytotoxic Activities and Total Phenolic Content of Four Indonesian Medicinal Plants

2012 ◽  
Vol 2 (4) ◽  
Author(s):  
Waras Nurcholis ◽  
Bambang Pontjo Priosoeryanto ◽  
Edy Djauhari Purwakusumah ◽  
Takeshi Katayama ◽  
Toshisada Suzuki
Keyword(s):  
Free Radical ◽  
Medicinal Plants ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Ic50 Values ◽  
Ethanol Extracts

The crude ethanol extracts of four Indonesian medicinal plants namely Curcuma xanthorrhiza Roxb.,Phyllanthus niruri Linn., Andrographis paniculata Ness., and Curcuma aeruginosa Roxb. wereexamined for their antioxidant (radical scavenging) activity using 2, 2-diphenyl-2-picrylhydrazyl(DPPH) free radical and cytotoxicity using brine shrimp lethality test (BSLT). The total phenoliccontent was used the Folin-Ciocalteu method. IC50 values for DPPH radical scavenging activityranged from 14.5 to 178.5 μg/ml, with P. niruri having the lowest value and therefore the mostpotent, and C. aeruginosa having the highest value. LC50 values for BSLT ranged from 210.3 to593.2 μg/ml, with C. xanthorrhiza and A. paniculata having the lowest and highest values,respectively. The total phenolic content of the Indonesian plants ranged from 133.0 ±3.7 to863.3±54.7 mg tannic acid equivalent per 1 g extract, with C. aeruginosa and P. niruri having thelowest and highest values, respectively. A positive correlation between free radical scavengingactivity and the content of phenolic compounds was found in the four of Indonesian medicinal plants.

scholarly journals Preliminary Screening of Corn Silk (Zea mays L.) for Antioxidant Properties and Cytotoxicity Activity on Human Myelogenous Leukemia Cell Line

2020 ◽  
Vol 2 (1) ◽  
pp. 52
Keyword(s):  
Ethyl Acetate ◽  
Mtt Assay ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Myelogenous Leukemia ◽  
Leukemia Cell Line ◽  
Total Phenolic ◽  
Cytotoxicity Activity ◽  
Corn Silk ◽  
Ic50 Value

One of the traditionally used herbs is the byproduct of the maize plant, the 10-20 cm long corn silk which from the female maize flowers. The aim of this study is to evaluate the total phenolic content, antioxidant activity, and cytotoxicity activity of corn silk. The corn silk was minced and was extracted with methanol-water (80 % v/v), methanol, ethanol, ethyl acetate, and hexane by using the maceration method. The total phenolic content (TPC) of corn silk was determined to assess the presence and level of phenolic compounds in each sample. The antioxidant activities of all corn silk extracts were determined via DPPH method, and MTT assay was used to study the viability of the cells after the cells were treated with corn silk extracts at different time intervals. The highest phenolic content was exhibited by the methanol extract. The EC50 value for methanol-water (80 % v/v), methanol, ethanol, ethyl acetate and hexane extracts were 251 μg/ ml, 300 μg/ ml, 330 μg/ ml, 550 μg/ ml and 1736 μg/ ml respectively. The MTT assay, the lowest IC50 values at 24 and 48 hours intervals, was exhibited by methanol-water extract (104 μg/ ml). In contrast, methanol (308 μg/ ml) was found with the highest IC50 value for all 24, 48, and 72 hours intervals. At 72 hours interval, ethyl acetate (88 μg/ ml) shown the lowest IC50 value. This study suggested that corn silk could be potentially used as a source of antioxidant and can further evaluate for cancer studies.

scholarly journals In Vitro Antioxidant, Cytotoxic Activities, and Phenolic Profile of Senecio glaucus from Saudi Arabia

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Ali S. Alqahtani ◽  
Rashed N. Herqash ◽  
Omar M. Noman ◽  
Fahd A. Nasr ◽  
Nouf Alyhya ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Side Effects ◽  
Cytotoxic Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Hplc Method ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Chloroform Fraction ◽  
Promising Source

Current treatments for complex diseases have remarkable side effects that negatively impact patients’ quality of life. Thus, natural compounds with fewer side effects represent a promising source for safe drugs. The genus Senecio is widely used in folk medicine due to its various pharmacological properties. In the present study, the total phenolic content of Senecio glaucus, which is grown in Saudi Arabia, was assessed using the Folin-Ciocalteau colorimetric method. Scavenging DPPH and ABTS assays were utilized to determine the antioxidant properties of S. glaucus fractions, and MTT assay was used to screen the cytotoxic activity of S. glaucus against various cancer cells. In addition, HPLC-UV was utilized to detect the presence of two phenolic acids, namely, vanillic acid (VA) and gallic acid (GA). Among all fractions tested, S. glaucus chloroform fraction (SGCF) yielded the highest value (125.3 mg·GA/g) in terms of total phenolic content. SGCF also exhibited the highest scavenging activities (76.7 and 74.1%) on both DPPH and ABTS assays, respectively. Similarly, SGCF also possessed the most potent cytotoxic activity against the MCF-7 cell line, with an IC50 value of 41.8 μg/ml. The validated HPLC method confirmed the presence of VA (4.8 μg/mg DW) and GA (3.9 μg/mg DW) in SGCF. Overall, our data show that S. glaucus had antioxidant and cytotoxic properties. A developed validated HPLC method which could be helpful for quantifying phenolic compounds in S. glaucus was established.

Antimicrobial activity, total phenolic content and flavonoid concentrations of Teucrium species

2012 ◽  
Vol 7 (4) ◽  
pp. 664-671 ◽  
Author(s):  
Milan Stanković ◽  
Olgica Stefanović ◽  
Ljiljana Čomić ◽  
Marina Topuzović ◽  
Ivana Radojević ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Total Phenolic Content ◽  
Antimicrobial Agents ◽  
Phenolic Content ◽  
Fungal Species ◽  
Total Phenolic ◽  
Total Phenols ◽  
Further Development ◽  
Ethyl Acetate Extracts

AbstractIn vitro antimicrobial activity of 21 crude extracts obtained from seven taxa of the genus Teucrium (T. chamaedrys, T. montanum, T. arduini, T. polium, T. scordium subsp. scordium, T. scordium subsp. scordioides and T. botrys) was tested against bacterial and fungal species. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined using a microdilution analysis method. Total phenolic content and flavonoid concentrations were measured spectrophotometrically. Total phenols were determined using Folin-Ciocalteu reagent and their amounts ranged from 28.49 up to 159.84 mg CA/g of extract (chlorogenic acid equivalent). The amounts of flavonoids ranged from 38.17 up to 190.45 mg RU/g of extract (rutin equivalent).The plant extracts showed greater potential of antibacterial than antifungal activity. A relationship was found between total phenolics and biological activity. The highest level of total phenols was measured in the methanol extracts, which demonstrated higher antimicrobial activity than acetone and ethyl acetate extracts. Staphylococcus aureus ATCC 25923 appeared to be the most sensitive organism. Our results indicate that Teucrium spp extracts are rich sources of phenolic compounds and are promising candidates for further development as natural antimicrobial agents.

scholarly journals Uji Aktivitas Antioksidan dan Fenolik Total Fraksi Etil Asetat Daun Sembung (Blumea balsamifera (L.) DC)

2021 ◽  
Vol 12 (2) ◽  
Author(s):  
Azimatur Rahmi ◽  
Tika Afriani ◽  
Linda Hevira ◽  
Wike Widiawati
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Total Phenolic ◽  
Activity Assay ◽  
Ic50 Value

The aim of this study is to investigate antioxidant activity and total phenolic content of ethyl acetate fraction of Sembung leaves. Sembung (Blumea balsamifera L.) is a plant that has the potential as an antioxidant because it contains secondary metabolites of alkaloids, steroids, flavonoids, saponins, and phenolic compounds. In this study, antioxidant activity was tested by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) for free radical scavenging activity assay. Evaluation of total phenolic content was tested by using the Folin-Ciocalteau method. The results showed that the ethyl acetate fraction of Sembung leaves possess showed medium antioxidant activity with IC50 valueof 221.821 compare with reference standard ascorbic acid with IC50 value 68.25 ppm. The ethyl acetate fraction of Sembung leaves has a total phenolic content of 161.101 mg GAE/g. This study provided that Blumea balsamifera leaves possess antioxidant.

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