scholarly journals Protective Effects of Melatonin on Saccharomyces cerevisiae under Ethanol Stress

Antioxidants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1735
Author(s):  
Mercè Sunyer-Figueres ◽  
Albert Mas ◽  
Gemma Beltran ◽  
María-Jesús Torija
Keyword(s):  
Oxidative Stress ◽  
Saccharomyces Cerevisiae ◽  
Lipid Peroxidation ◽  
Cell Growth ◽  
Antioxidant Enzyme ◽  
Alcoholic Fermentation ◽  
Yeast Cells ◽  
Ethanol Stress ◽  
Antioxidant Enzyme Activities ◽  
Ros Accumulation

During alcoholic fermentation, Saccharomyces cerevisiae is subjected to several stresses, among which ethanol is of capital importance. Melatonin, a bioactive molecule synthesized by yeast during alcoholic fermentation, has an antioxidant role and is proposed to contribute to counteracting fermentation-associated stresses. The aim of this study was to unravel the protective effect of melatonin on yeast cells subjected to ethanol stress. For that purpose, the effect of ethanol concentrations (6 to 12%) on a wine strain and a lab strain of S. cerevisiae was evaluated, monitoring the viability, growth capacity, mortality, and several indicators of oxidative stress over time, such as reactive oxygen species (ROS) accumulation, lipid peroxidation, and the activity of catalase and superoxide dismutase enzymes. In general, ethanol exposure reduced the cell growth of S. cerevisiae and increased mortality, ROS accumulation, lipid peroxidation and antioxidant enzyme activity. Melatonin supplementation softened the effect of ethanol, enhancing cell growth and decreasing oxidative damage by lowering ROS accumulation, lipid peroxidation, and antioxidant enzyme activities. However, the effects of melatonin were dependent on strain, melatonin concentration, and growth phase. The results of this study indicate that melatonin has a protective role against mild ethanol stress, mainly by reducing the oxidative stress triggered by this alcohol.

scholarly journals Effect of Exogenous Treatment with Nitric Oxide (NO) on Redox Homeostasis in Barley Seedlings (Hordeum vulgare L.) Under Copper Stress

2022 ◽  
Author(s):  
Marouane Ben Massoud ◽  
Oussama Kharbech ◽  
Yathreb Mahjoubi ◽  
Abdelilah Chaoui ◽  
Astrid Wingler
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Lipid Peroxidation ◽  
Hordeum Vulgare ◽  
Antioxidant Enzyme ◽  
Enzyme Activities ◽  
Redox Homeostasis ◽  
Copper Stress ◽  
Antioxidant Enzyme Activities ◽  
Cu Toxicity

AbstractThe protective mechanism of nitric oxide (NO) in regulating tolerance to Cu-induced toxicity in shoots of barley (Hordeum vulgare L.) was studied. The experiment consisted of four treatments based on additions to basal nutrient solutions (BNS): control (CTR), Cu (200 µM), SNP (500 µM), and Cu (200 µM) + SNP (500 µM) over a period of 10 days. Treatment with Cu significantly reduced seedling growth and photosynthetic efficiency concomitant with an increase in reactive oxygen species contents, lipid peroxidation markers, and antioxidant enzyme activities, indicating that Cu induced oxidative stress. Furthermore, growth inhibition of Cu-treated plants was associated with a reduction in photosynthetic pigments and maximum photosystem II efficiency as well as a strong decrease in levels of glutathione (GSH) and ascorbate (AsA). Addition of a nitric oxide (NO) donor, sodium nitroprusside (SNP), to the growth medium alleviated Cu toxicity by decreasing Cu uptake and enhancing antioxidant capacity, as indicated by increased contents of GSH and AsA. The application of SNP decreased oxidative stress and lipid peroxidation by suppressing lipoxygenase activity and enhancing some antioxidant enzyme activities. The results obtained indicate the potential of exogenously applied SNP in the management of metal toxicity. Hence, NO generating compounds have potential agronomical applications when cultivating in contaminated areas. Our findings indicate that NO can alleviate Cu toxicity by affecting the antioxidant defense system and maintaining the glutathione-ascorbate cycle status, suggesting that SNP treatment protects proteins against oxidation by regulating the cellular redox homeostasis.

Antioxidant enzyme activities and lipid peroxidation in Mytilus galloprovincialis from the French Mediterranean coast

2010 ◽  
Vol 39 (4) ◽  
Author(s):  
Krzysztof Gwoździński ◽  
Marta Gonciarz ◽  
Ewa Kilańczyk ◽  
Aleksandra Kowalczyk ◽  
Anna Pieniążek ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Oxidative Damage ◽  
Antioxidant Enzyme ◽  
Digestive Gland ◽  
Enzyme Activities ◽  
Mytilus Galloprovincialis ◽  
Mediterranean Coast ◽  
Antioxidant Enzyme Activities ◽  
Gland Tissue ◽  
Two Populations

Antioxidant enzyme activities and lipid peroxidation inIn the present work we have studied some of the indicators of oxidative damage of the digestive gland tissue of two populations of mussels

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