scholarly journals Wolfberry (Lycium barbarum) Consumption with a Healthy Dietary Pattern Lowers Oxidative Stress in Middle-Aged and Older Adults: A Randomized Controlled Trial

Antioxidants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 567
Author(s):  
Darel Wee Kiat Toh ◽  
Wan Yee Lee ◽  
Hanzhang Zhou ◽  
Clarinda Nataria Sutanto ◽  
Delia Pei Shan Lee ◽  
...  

Incorporating zeaxanthin-rich wolfberry (Lycium barbarum) into a healthy dietary pattern may augment its antioxidant potential. The present 16-week, parallel design randomized controlled trial aimed to investigate the impact of adhering to a healthy dietary pattern, either with or without whole dried wolfberry (15 g/d) on oxidative stress status (plasma malondialdehyde and 8-iso-prostaglandin F2α) in middle-aged and older adults. Changes to carotenoids status (plasma and skin carotenoids) and body composition were further evaluated to explore potential mechanisms which underlie the antioxidant properties of wolfberry. Plasma 8-iso-prostaglandin F2α, plasma zeaxanthin and skin carotenoids status were significantly raised in the wolfberry consuming group (n = 22; p < 0.05) compared to the control group which showed no changes (n = 18). Likewise in the wolfberry group only, inverse association was observed between the change values of plasma zeaxanthin and plasma 8-iso-prostaglandin F2α (−0.21 (−0.43, 0.00) ng/µmol, regression coefficient (95% CI); p = 0.05). Wolfberry consumption with a healthy dietary pattern may serve as a dietary strategy to attenuate lipid peroxidation among middle-aged and older adults who are at a heightened risk of oxidative stress induced age-related disorders. The antioxidant properties of wolfberry may be attributed to its rich zeaxanthin content.

2021 ◽  
Vol 5 (Supplement_2) ◽  
pp. 79-79
Author(s):  
Darel Wee Kiat Toh ◽  
Wan Yee Lee ◽  
Hanzhang Zhou ◽  
Clarinda Sutanto ◽  
Delia Pei Shan Lee ◽  
...  

Abstract Objectives The incorporation of zeaxanthin rich wolfberry (Lycium barbarum) into a healthy dietary pattern may augment its antioxidant effects although evidence evaluating whole fruits is lacking. The objectives of this study are to (1) investigate the impact of dietary counselling, either with or without whole dried wolfberry on oxidative stress status in middle-aged and older Singaporean adults and (2) delineate underlying mechanisms by examining associations with the corresponding changes in carotenoids status. Methods In this 16-week, parallel design randomized controlled trial, 40 subjects (29 F, 11 M; aged 50 to 64 y) received dietary counselling to follow a healthy dietary pattern. Compared to the control group (CG, n = 18), the wolfberry group (WG, n = 22) additionally cooked and consumed 15 g/d whole dried wolfberry with their main meals. Biomarkers of oxidative stress (plasma malondialdehyde (MDA) by thiobarbituric acid reactive substance assay and 8-isoprostanes by ELISA) were measured at baseline and week 16. Plasma and dietary carotenoids analyzed by HPLC and 3-day food records respectively were measured at baseline, week 8 and week 16 while skin carotenoids status (SCS) by resonance Raman spectroscopy was assayed every 4 weeks. Results Plasma 8-isoprostanes showed a time effect (P &lt; 0.05), attributed to the marked decline in the WG only (WG: −19.0 ± 43.6 ng/L; CG: −7.7 ± 38.7 ng/L, mean ± SD) though no effect was observed for MDA. A significant interaction effect (P &lt; 0.001), driven by higher plasma zeaxanthin (WG: +0.08 ± 0.13 nmol/L; CG: −0.03 ± 0.05 nmol/L) and SCS (WG: +4242 ± 4938 a.u.; CG: +1713 ± 5921 a.u.) was observed in the WG with a marked increase evident from week 8 onwards although concentrations of the other plasma carotenoids were maintained. Moreover, the change value for plasma zeaxanthin in the WG was inversely associated with the corresponding changes in plasma 8-isoprostanes (−0.21 (−0.43, 0.00) ng/µmol, regression coefficient (95% CI); P = 0.05). This same association was absent in the CG. Conclusions Consuming wolfberry with a healthy dietary pattern attenuates oxidative stress in middle-aged and older adults and this may be attributed to the rich zeaxanthin content in wolfberry. Funding Sources National University of Singapore Ministry of Education, Singapore Agency for Science, Technology and Research.


2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 82-82
Author(s):  
Darel Wee Kiat Toh ◽  
Xuejuan Xia ◽  
Jasmine Hui Min Low ◽  
Clarinda Sutanto ◽  
Wan Yee Lee ◽  
...  

Abstract Objectives The study aimed to investigate the impact of wolfberry (Lycium barbarum) consumption as part of a healthy dietary pattern on cardiovascular health in Singaporean middle-aged and older adults. It was hypothesized that the consumption of wolfberry could further enhance the cardiovascular protective effects of a healthy dietary pattern. Methods This was a 16 week, parallel design, randomized controlled trial where 40 Singaporean men and women (aged 50 to 64 years) received dietary counselling to follow a healthy dietary pattern either with or without 15 g/day of dried whole wolfberry, cooked and consumed as part of their meals. Blood pressure, serum lipid-lipoprotein concentrations and dietary compliance using 3-day food records were monitored every 4 weeks. Further evaluation of cardiovascular disease (CVD) biomarkers, broadly classified as serological (total nitrate/nitrite, endothelin-1, intercellular adhesion molecule-1, angiopoietin-1, angiopoietin-2 and von-Willebrand factor), structural (carotid intima-media thickness using B-mode ultrasonography) and functional (flow-mediated dilation using B-mode ultrasonography and circulating endothelial progenitor cells (CD34+/KDR+) by fluorescence-activated cell sorting) were analyzed before and after intervention. Results Adherence to a healthy dietary pattern contributed to a time dependent effect on both the plasma total nitrate/nitrite (P &lt; 0.01) and plasma endothelin-1 (P &lt; 0.005) which were raised and lowered respectively at week 16. However, changes were significant only in the wolfberry group (total nitrate/nitrite: 15.9 ± 1.8 to 19.4 ± 2.2 μmol/L, P &lt; 0.05; endothelin-1: 1.31 ± 0.12 to 1.11 ± 0.10 ng/L, P &lt; 0.01) and not in the control group. Moreover, a significant increase in serum high density lipoprotein (HDL) cholesterol was also detected solely in the wolfberry group (1.56 ± 0.10 to 1.65 ± 0.10 mmol/L, P &lt; 0.05). The other serological, structural and functional biomarkers of cardiovascular health showed no observable change after the intervention. Conclusions Incorporating wolfberry to your daily meals may augment the cardiovascular protective benefits of a healthy dietary pattern by improving the regulation of vascular tone and plasma lipid-lipoprotein profile in Singaporean middle-aged and older adults. Funding Sources Ministry of Education, Singapore.


2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 90-90
Author(s):  
Xuejuan Xia ◽  
Darel Wee Kiat Toh ◽  
Kian Keong Poh ◽  
Roger S Y Foo ◽  
Shi Ling Ng ◽  
...  

Abstract Objectives Endothelial progenitor cells (EPCs), a novel biomarker of cardiovascular disease, are involved in the maintenance of endothelial homeostasis and vascular repair and the number of EPCs could be altered by dietary modification. Wolfberry is widely known for the potential cardiovascular benefits, however, its impact on EPC count and function is not reported. This study aims to investigate the effect of consuming wolfberry as part of a healthy dietary pattern (HDP) on the number and function of EPCs derived from blood (BOECs). Methods Twenty-four subjects from Singapore were randomized and consumed either HDP only (HDPO; n = 9; mean age 55 ± 3 years) or HDP supplemented with 15 g/day wolfberry (HDPW; n = 15; mean age 56 ± 4 years) for 16 weeks. At pre- and post-intervention, 27 mL peripheral blood was collected from subject for the isolation of BOECs. Immunofluorescence staining with von Willebrand factor (VWF) and flow cytometry analysis with cell surface markers including KDR and CD34 were performed to verify the obtained BOECs. In-vitro tube formation assay was performed to measure the angiogenic properties of BOECs. Endothelial-dependent flow-mediated dilation (FMD) and intima-media thickness (IMT) were also measured by ultrasonographic imaging. Results Isolated BOECs have typical endothelial cobblestone morphology, express VWF and KDR, and partly express CD34. Compared with pre-intervention, both HDPO and HDPW groups showed an increase in total colony numbers of BOECs (from 1.8 ± 0.5 to 3.1 ± 1.1, P = 0.28 and 2.7 ± 0.8 to 3.2 ± 0.6, P = 0.51, respectively). For the tube formation capacity of BOECs, adherence to a HDP contributed to a time effect on both the total mesh area (P = 0.02) and mean mesh size (P &lt; 0.01). However, further improvement in total master segment length (6.9 ± 1.6 to 11.4 ± 1.0 mm, P = 0.03), total mesh area (0.2 ± 0.1 to 0.7 ± 0.1 mm2, P = 0.03), and mean mesh size (0.02 ± 0.006 to 0.05 ± 0.003 mm2, P &lt; 0.001) were only observed in the HDPW group compared with pre-intervention and not in the HDPO group. No changes of FMD and IMT were detected after intervention. Conclusions Adherence to a HDP increases colony count and enhances angiogenic function of BOECs, and the angiogenic capability of BOECs is further improved by wolfberry supplement in Singaporean middle-aged and older adults. Funding Sources Ministry of Education, Singapore.


2021 ◽  
Author(s):  
Xuejuan Xia ◽  
Darel Wee Kiat Toh ◽  
Shi Ling Ng ◽  
Olga Zharkova ◽  
Kian Keong Poh ◽  
...  

Blood outgrowth endothelial cells (BOECs) have received growing attention in relation to the cardiovascular disease (CVD). However, the effect of diet intervention, a primary strategy for CVD prevention on BOECs...


2020 ◽  
Vol 96 ◽  
pp. 106105
Author(s):  
Manfred Diehl ◽  
Abigail Nehrkorn-Bailey ◽  
Katherine Thompson ◽  
Diana Rodriguez ◽  
Kaigang Li ◽  
...  

10.2196/14969 ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. e14969
Author(s):  
Jing Liao ◽  
Hai-Yan Xiao ◽  
Xue-Qi Li ◽  
Shu-Hua Sun ◽  
Shi-Xing Liu ◽  
...  

Background Wearable activity trackers offer potential to optimize behavior and support self-management. To assist older adults in benefiting from mobile technologies, theory-driven deployment strategies are needed to overcome personal, technological, and sociocontextual barriers in technology adoption. Objective To test the effectiveness of a social group–based strategy to improve the acceptability and adoption of activity trackers by middle-aged and older adults. Methods A cluster randomized controlled trial was conducted among 13 groups of middle-aged and older adults (≥45 years) performing group dancing (ie, square dancing) as a form of exercise in Guangzhou from November 2017 to October 2018. These dancing groups were randomized 1:1 into two arms, and both received wrist-worn activity trackers and instructions at the baseline face-to-face assessment. Based on the Information-Motivation-Behavior Skill framework, the intervention arm was also given a tutorial on the purpose of exercise monitoring (Information), encouraged to participate in exercise and share their exercise records with their dancing peers (Motivation), and were further assisted with the use of the activity tracker (Behavior Skill). We examined two process outcomes: acceptability evaluated by a 14-item questionnaire, and adoption assessed by the uploaded step count data. Intention-to-treat analysis was applied, with the treatment effects estimated by multilevel models. Results All dancing groups were followed up for the postintervention reassessment, with 61/69 (88%) participants of the intervention arm (7 groups) and 56/80 (70%) participants of the control arm (6 groups). Participants’ sociodemographic characteristics (mean age 62 years, retired) and health status were comparable between the two arms, except the intervention arm had fewer female participants and lower cognitive test scores. Our intervention significantly increased the participants’ overall acceptability by 6.8 points (95% CI 2.2-11.4), mainly driven by promoted motivation (adjusted group difference 2.0, 95% CI 0.5-3.6), increased usefulness (adjusted group difference 2.5, 95% CI 0.9-4.1), and better perceived ease of use (adjusted group difference 1.2, 95% CI 0.1-2.4), whereas enjoyment and comfort were not increased (adjusted group difference 0.9, 95% CI –0.4-2.3). Higher adoption was also observed among participants in the intervention arm, who were twice as likely to have valid daily step account data than their controlled counterparts (adjusted incidence relative risk [IRR]=2.0, 95% CI 1.2-3.3). The average daily step counts (7803 vs 5653 steps/day for the intervention and control, respectively) were similar between the two arms (adjusted IRR=1.4, 95% CI 0.7-2.5). Conclusions Our social group–based deployment strategy incorporating information, motivation, and behavior skill components effectively promoted acceptability and adoption of activity trackers among community-dwelling middle-aged and older adults. Future studies are needed to examine the long-term effectiveness and apply this social engagement strategy in other group settings or meeting places. Trial Registration Chinese Clinical Trial Registry ChiCTR-IOC-17013185; https://tinyurl.com/vedwc7h.


2019 ◽  
Vol 10 (2) ◽  
pp. 1062-1072
Author(s):  
Negin Navaei ◽  
Shirin Pourafshar ◽  
Neda S. Akhavan ◽  
Nicole S. Litwin ◽  
Elizabeth M. Foley ◽  
...  

Previous research suggests potential for fresh pears as a functional food for promoting cardiometabolic health.


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