scholarly journals Protective Effect of Resveratrol Improves Systemic Inflammation Responses in LPS-Injected Lambs

Animals ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 872 ◽  
Author(s):  
Yanping Liang ◽  
Jianwei Zhou ◽  
Kaixi Ji ◽  
Hu Liu ◽  
Allan Degen ◽  
...  
Keyword(s):  
Inflammatory Cytokines ◽  
Inflammatory Responses ◽  
Clinical Symptoms ◽  
Average Daily Gain ◽  
Clinical Signs ◽  
Blood Parameters ◽  
Mapk Signaling ◽  
Inflammatory Genes ◽  
Expression Levels ◽  
Daily Gain

Highly intensive livestock production often causes immune stress to animals, which makes them more susceptible to infections. The aim of this study was to examine whether resveratrol (Res) alleviates inflammation in lambs. In Experiment 1, 16 male lambs were injected with lipopolysaccharides (LPS) at an initial dose of 0.25, 1.25, and 2.5 μg/kg body weight (BW) for 9 days. Average daily gain and blood parameters were measured and clinical symptoms were recorded. In Experiment 2, 20 male lambs were injected intravenously with LPS (0 mg/kg) + Res (0 mg), LPS (2.5 μg /kg) + Res (0 mg, 82.5 mg, 165 mg, 330 mg), 4 h after LPS injection. Jugular blood was collected from each lamb to determine white blood cell (WBC) counts and the expression of inflammatory genes. In Experiment 1, all LPS-treated lambs showed clinical signs of sickness including rhinorrhea, lethargy, and shivering, and systemic inflammatory responses of increased inflammatory genes levels and cortisol concentration. The lambs had increased respiratory and heart rates and rectal temperature and decreased average daily gain and feed intake. In Experiment 2, resveratrol significantly reduced WBCs and the expression levels of several genes associated with inflammation response (TLR4, NF-κB, c-jun) and inhibited the signaling cascades of NF-κB and MAPKs by down-regulating the expression levels of inflammatory cytokines (IL-1β, IL-4, IL-6, TNF-α, IFN-γ) induced by LPS. Resveratrol attenuated the LPS-evoked inflammatory responses in lambs by suppressing expression levels of inflammatory cytokines, and blocking NF-κB and MAPK signaling pathways.

scholarly journals The influence of the probiotic “Active East” on the digestibility of nutrients, morphological and biochemical blood parameters of young pigs

2019 ◽  
pp. 79-82
Author(s):  
Sergey Petrovich Moskalenko ◽  
Roman Phedorovich Belov
Keyword(s):  
Biochemical Parameters ◽  
Average Daily Gain ◽  
Live Weight ◽  
Blood Parameters ◽  
Young Pigs ◽  
Probiotic Preparation ◽  
Daily Gain ◽  
Fattening Pigs

The results of the experiment on feeding the probiotic “Active East”  to young fattening pigs are presented. Its inclusion in the diet of animals stimulated an increase in the digestibility of the main nutrients of the diet while increasing the gross and average daily gain in live weight. The use of a probiotic preparation did not adversely affect the morphological and biochemical parameters of the blood.  

scholarly journals Evaluate the Therapeutic Effect of Allicin (L-cysteine) on Clinical Presentation and Prognosis in Patients with COVID-19

2021 ◽  
Author(s):  
Hosein Yaghoubian ◽  
Hossein Niktale ◽  
Arash Peivandi Yazdi ◽  
Vahideh Ghorani ◽  
Masoud Mahdavi Rashed ◽  
...  
Keyword(s):  
Therapeutic Effect ◽  
Clinical Presentation ◽  
Clinical Symptoms ◽  
Healing Process ◽  
Clinical Signs ◽  
Signs And Symptoms ◽  
Blood Parameters ◽  
Radiological Data ◽  
Clinical Signs And Symptoms ◽  
Chest Ct Scan

The antiviral effectiveness of allicin (L-cysteine) has been shown by numerous studies in both levels of clinical and animals. The aim of this study was to evaluate the therapeutic effect of allicin (L-cysteine) on clinical presentation and prognosis. In the current study, 66 patients with COVID-19 based on clinical, radiological presentations and RT-PCR results, were enrolled in two groups of placebo and allicin. In the both allicin (L-cysteine) and placebo groups (n=33 in each group), the capsules were prescribed two times a day for two weeks. Clinical signs and symptoms, blood parameters and chest CT scan were evaluated before and two weeks after treatment. The results showed that allicin (L-cysteine) could significantly impact on improvement of signs and symptoms of COVID-19 after two weeks of treatment in comparison to placebo. Allicin (L-cysteine) not only improve the clinical signs, but also ameliorate the lab and radiological data, which suggest a therapeutic effect for this agent in COVID-19. Our data suggest the therapeutic effect of allicin (L-cysteine) on COVID-19 through improvement of clinical symptoms and acceleration of the healing process.

scholarly journals MiR-4792 regulates inflammatory responses in Cryptococcus neoformans infected microglia

2020 ◽  
Author(s):  
Jianghan Chen ◽  
Guotai Yao ◽  
Xiaoli Wang ◽  
Qing Hou ◽  
Rui Gao ◽  
...  
Keyword(s):  
Cryptococcus Neoformans ◽  
Inflammatory Cytokines ◽  
Cryptococcal Meningitis ◽  
Target Gene ◽  
Inflammatory Responses ◽  
Fungal Infections ◽  
Mapk Signaling ◽  
Microbial Infection ◽  
Bv2 Cells ◽  
Infected Cells

Abstract Background: Investigating the factors that influence inflammatory response of microglial cells is important to understand the pathogenesis of cryptococcal meningitis (CM). MicroRNA (miRNA) have been shown to play an important role in inducing host defenses and activating immune response in the process of microbial infection; however, the regulatory mechanisms of miRNAs in cryptococcal meningitis are poorly defined. In our previous analysis, we assessed the miRNA profiles of BV2 cells following Cryptococcus neoformans (C. neoformans) infection. In this study, we characterized the expression of miR-4792 in CM patients to further our understanding of the host response to pathogen infections. Results: miR-4792 was downregulated in BV2 cells infected with C. neoformans while its target gene EGFR was upregulated. Infected cells with up-regulated miR-4792 exhibited a trend towards decreased EGFR transcript expression, reduced MAPK signaling and a decreased secretion of inflammatory cytokines. Afterantifungal treatment in cryptococcal meningitis patients, the levels of miR-4792 in the CSF significantly increased, while the expression of EGFR significantly decreased. Conclusion: This study identified that miR-4792 and its target gene EGFR regulate the secretion of inflammatory cytokines in BV2 cells infected with C. neoformans. This furthers our knowledge of the inflammatoryresponses to fungal infections in the CNS.

scholarly journals The Prophylactic Use of Bovine Colostrum in a Murine Model of TNBS-Induced Colitis

Animals ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 492 ◽  
Author(s):  
Laura Menchetti ◽  
Giulio Curone ◽  
Iulia Elena Filipescu ◽  
Olimpia Barbato ◽  
Leonardo Leonardi ◽  
...  
Keyword(s):  
Clinical Symptoms ◽  
Clinical Signs ◽  
Interleukin 10 ◽  
Bovine Colostrum ◽  
Saline Control ◽  
Control Group ◽  
Microbial Composition ◽  
Expression Levels ◽  
Pre Treatment ◽  
Prophylactic Use

This study investigated the effects of a short-term administration of bovine colostrum (BC) in a TNBS model of induced colitis. Colitis was induced by TNBS treatment after seven days of BC (BC group, n = 12) or saline (control group, n = 12) administration in mice. Clinical signs, histopathological characteristics, expression levels of Toll-like receptor 4 (TLR4), pro- and anti-inflammatory cytokines, and microbial composition were assessed. BC was well tolerated and did not induce any histological damage or clinical symptoms. After TNBS treatment, the BC group showed a reduction in body weight (BW) loss compared to Control (p < 0.05). Moreover, expression levels of TLR4 (p < 0.01), Interleukin-1β (IL-1β; p < 0.001), Interleukin-8 (IL-8; p < 0.001), and Interleukin-10 (IL-10; p < 0.001) were lower in mice administered with BC. Finally, Escherichia coli were higher (p < 0.05), while Enterococci (p < 0.001), Lactobacillus spp. (p < 0.001), and Bifidobacterium spp. (p < 0.05) were lower in Control than BC group. This study confirms that pre-treatment with BC modulates the expression of genes and the count of microbes involved in the etiopathogenesis of colitis.

scholarly journals Intrauterine inflammation induced white matter injury protection by fibrinogen-like protein 2 deficiency in perinatal mice

2020 ◽  
Author(s):  
Di Zhan ◽  
Cai Zhang ◽  
Wenjun Long ◽  
Lan Wei ◽  
Shengjuan Jin ◽  
...  
Keyword(s):  
Body Weight ◽  
White Matter ◽  
Inflammatory Responses ◽  
Body Weight Loss ◽  
Mapk Signaling ◽  
Mitogen Activated Protein Kinase ◽  
White Matter Injury ◽  
List Type ◽  
Expression Levels ◽  
Microglial Polarization

Abstract Background White matter injury (WMI) induced by intrauterine inflammation can cause adverse neurological outcomes. Fibrinogen-like protein 2 (FGL2)/fibroleukin is an important trigger of inflammatory responses and is involved in some cerebral diseases. However, the role of FGL2 in intrauterine inflammation-induced WMI remains unclear. Methods Lipopolysaccharide (LPS) was intraperitoneally injected into wild-type and FGL2 knockout mice to induce intrauterine inflammation. Body weight and brain weight of offspring were monitored. Major basic protein (MBP) expression was evaluated to demonstrate the myelination of offspring. To investigate the regulatory mechanism of FGL2, cytokine expression, microglial polarization, and the activation of mitogen-activated protein kinase (MAPK) signaling pathway in the offspring were analyzed. Results Upon LPS exposure, FGL2 knockout offspring showed a significant increase in body weight loss. MBP reduction induced by LPS was prevented in FGL2 knockout offspring. Expression levels of proinflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α, and M1 marker CD86 were suppressed, while the expression levels of anti-inflammatory cytokines IL-10 and M2 marker CD206 were increased. FGL2 deficiency significantly inhibited the phosphorylation of p38MAPK and c-Jun N-terminal kinase (JNK) protein. Conclusions FGL2 deficiency can ameliorate WMI induced by intrauterine inflammation, reducing inflammatory cascade and improving hypomyelination, through the regulation of microglial polarization and MAPK signaling pathways. Impact Intrauterine inflammation induces WMI leading to severe neurological sequelae. FGL2 plays an important role in the progression of WMI induced by intrauterine inflammation. FGL2 deficiency can protect against WMI by inhibiting p38 MAPK and JNK phosphorylation, regulating microglia polarization, and reducing inflammation response. FGL2 could be a novel molecular target for protecting against WMI induced by intrauterine inflammation.

Effects of energy sources and inclusion levels of concentrate in sugarcane silage-based diets of finishing Nellore young bulls: Feeding behaviour, performance and blood parameters

2019 ◽  
Vol 157 (04) ◽  
pp. 350-356
Author(s):  
V. B. Ferrari ◽  
N. R. B. Cônsolo ◽  
R. T. Sousa ◽  
J. M. Souza ◽  
M. H. A. Santana ◽  
...  
Keyword(s):  
Feeding Behaviour ◽  
Block Design ◽  
Average Daily Gain ◽  
Blood Parameters ◽  
Carcass Weight ◽  
Carbohydrate Supplementation ◽  
Randomized Block Design ◽  
Large Particles ◽  
Final Weight ◽  
Daily Gain

AbstractReplacing ground maize (GM) with steam-rolled maize typically increases feed efficiency in maize-silage-based diets. However, little is known about optimal carbohydrate supplementation in sugarcane silage-based diets. The objective was to quantify the effect of partially replacing GM with steam-rolled maize (SRM) or pelleted citrus pulp (PCP) at two concentrate levels (600 or 800 g/kg DM) in sugarcane-based diets on feeding behaviour, performance and blood parameters of finishing Nellore bulls. One hundred and eight young bulls were allocated to 36 pens in a randomized block design and fed for 84 d. Feeding 800 g/kg concentrate decreased time spending eating and ruminating, but improved G:F ratio, hot carcass weight and carcass dressing, compared to 600 g/kg concentrate. Bulls fed SRM and PCP diets with 600 g/kg concentrate had lower intake compared to GM. Both final weight and average daily gain decreased when bulls were fed PCP and SRM with 600 g/kg concentrate compared to GM diets, and when fed with PCP and 800 g/kg concentrate. Substituting PCP for GM decreased gain efficiency, carcass weight, rumination time and intake efficiency, indicating that the bulls consumed less feed per hour spent eating. Substituting SRM for GM increased backfat thickness and blood urea concentration. In conclusion, the replacement of GM with PCP reduces intake and enhances selection against large particles, decreasing rumination, performance and final carcass weight and dressing. Replacement of GM with SRM increases blood urea and fat deposition, with no impact on performance.

Effects of different concentrations of ground oak acorn on growth performance, blood parameters and carcass characteristics of goat kids

2015 ◽  
Vol 55 (1) ◽  
pp. 87 ◽  
Author(s):  
E. Froutan ◽  
O. Azizi ◽  
G. Sadeghi ◽  
F. Fatehi ◽  
S. Lashkari
Keyword(s):  
Growth Performance ◽  
Average Daily Gain ◽  
Carcass Characteristics ◽  
Blood Parameters ◽  
Abdominal Fat ◽  
Conversion Ratio ◽  
Feed Conversion ◽  
Low Concentrations ◽  
Daily Gain ◽  
Different Levels

The objective of the present study was to evaluate the effect of different levels of ground oak acorn on growth performance, blood parameters and carcass characteristics. Twenty-four goat kids averaging 16.93 ± 1.25 kg initial bodyweight were randomly assigned to four experimental diets in a completely randomised design for 90 days. The experimental diets consisted of (1) 0% oak acorn (control), (2) 8% oak acorn, (3) 17% oak acorn and (4) 25% oak acorn on a DM basis. The goats were slaughtered after 105 days of feeding and carcass characteristics were measured. DM intake increased linearly in small amounts as the level of acorn in the diet increased (P < 0.004). The results showed that average daily gain, water intake and feed conversion ratio were unaffected by levels of ground oak acorn (P > 0.05). There were no significant differences in blood parameters (P > 0.05). Carcass fat depth and abdominal fat weight decreased with increasing ground oak acorn in the diet. Other carcass characteristics were not affected by different levels of ground oak acorn. On the basis of the results of the present study, it may be concluded that ground oak acorn can be used up to 25% in the diets of young goats without any adverse effects on growth performance and carcass characteristics. At this level of acorns, the goats received low concentrations of hydrolysable tannins (11 g/kg DM) in their diet.

scholarly journals 15 Host genetics of antibody response to PRRS vaccination and infection in growing pigs

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 19-19
Author(s):  
Megan Neveau ◽  
Leticia P Sanglard ◽  
Jack C Dekkers ◽  
Erin Little ◽  
Scott A Dee ◽  
...  
Keyword(s):  
Antibody Response ◽  
Genetic Parameters ◽  
Virulent Strain ◽  
Average Daily Gain ◽  
Clinical Signs ◽  
Growing Pigs ◽  
Live Virus ◽  
Nursery Pigs ◽  
Prrs Virus ◽  
Daily Gain

Abstract Antibody response to Porcine Reproductive and Respiratory Syndrome (PRRS) vaccination and infection has been shown to be highly heritable in gilts and sows. This trait, measured as sample-to-positive (S/P) ratio, has been proposed as a selection tool for improved reproductive performance in sows. However, this novel trait has not yet been explored in growing pigs. Therefore, the objective of this study was to estimate genetic parameters for S/P in PRRS-challenged nursery pigs. A total of 854 PRRS-naïve crossbred barrows and gilts (51.9±4.8 d and 17.8±4.6 kg) were randomly allocated to 54 pens in two rooms and vaccinated with a modified live virus PRRS vaccine. Twenty-eight days later (d0), all animals were experimentally inoculated with a highly virulent strain of PRRS virus. Body weight (BW), S/P, and clinical signs of disease were recorded at this time and again at d13. Average daily gain (ADG) was calculated and used in combination with clinical signs to create two distinct groups, robust (R) and susceptible (S), with ~230 animals each. At d42, S/P was measured only in these classified animals. Genetic parameters and effect of classification were estimated for S/P for each collection day. Heritability estimates for S/P were 0.07±0.06, 0.52±0.09, and 0.43±0.28, for d0, d13, and d42, respectively. Genetic correlation estimates were high between collection days, ranging from 0.91±0.31 (d0 and d42) to 0.98±0.03 (d13 and d42). There was an effect of classification for S/P only at d13 (P=0.024), with R showing greater S/P (1.7±0.03) than S (1.64±0.03). These results indicate that, in PRRS-challenged growing pigs, S/P is highly heritable at later collection days, and differences in S/P exist between robust and susceptible animals.

scholarly journals Critical role for integrin-β4 in the attenuation of murine acute lung injury by simvastatin

2012 ◽  
Vol 303 (4) ◽  
pp. L279-L285 ◽  
Author(s):  
Weiguo Chen ◽  
Saad Sammani ◽  
Sumegha Mitra ◽  
Shwu Fan Ma ◽  
Joe G. N. Garcia ◽  
...  
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Inflammatory Cytokines ◽  
Inflammatory Responses ◽  
Critical Role ◽  
Mapk Signaling ◽  
Blue Dye ◽  
Blocking Antibody ◽  
Cell Counts ◽  
Integrin Β4

The statins are a class of 3-hydroxy-3-methylglutaryl-coenzyme A-reductase inhibitors that are recognized to have pleiotropic properties. We previously reported the attenuation of LPS-induced murine acute lung injury (ALI) by simvastatin in vivo and identified relevant effects of simvastatin on endothelial cell (EC) signaling, activation, and barrier function in vitro. In particular, simvastatin induces the upregulation of integrin-β4, which in turn inhibits EC inflammatory responses via attenuation of MAPK signaling. The role of integrin-β4 in murine ALI protection by simvastatin, however, is unknown. We initially confirmed a time- and dose-dependent effect of simvastatin on increased integrin-β4 mRNA expression in human lung EC with peak protein expression evident at 16 h. Subsequently, reciprocal immunoprecipitation demonstrated an attenuation of LPS-induced integrin-β4 tyrosine phosphorylation by simvastatin (5 μM, 16 h). Increased expression of EC inflammatory cytokines [IL-6, IL-8, monocyte chemoattractant protein (MCP)-1, regulated on activation normal T cell expressed and secreted (RANTES)] by LPS (500 ng/ml, 4 h) was also significantly attenuated by simvastatin pretreatment (5 μM, 16 h), but this effect was reversed by cotreatment with an integrin-β4-blocking antibody. Finally, although simvastatin (20 mg/kg) conferred significant protection in murine ALI as evidenced by decreased bronchoalveolar lavage fluid cell counts, protein, inflammatory cytokines (IL-6, IL-1β, MCP-1, RANTES), decreased Evans blue dye albumin extravasation in lung tissue, and changes on lung histology, these effects were reversed by the integrin-β4-blocking antibody (IV, 1 mg/kg, 2 h before LPS). These findings support integrin-β4 as an important mediator of ALI protection by simvastatin and implicate signaling by integrin-β4 as a novel therapeutic target in patients with ALI.

scholarly journals Weaning Performance of Beef Cattle Calves Based on Concentrate Intake

Animals ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 18
Author(s):  
Chong Wang ◽  
Dongping Li ◽  
Jinyong Yang ◽  
Yuefeng Xia ◽  
Yan Tu ◽  
...  
Keyword(s):  
Positive Impact ◽  
Average Daily Gain ◽  
Body Height ◽  
Blood Parameters ◽  
Fresh Milk ◽  
Southern Chinese ◽  
Body Weights ◽  
Total Antioxidant ◽  
Primary Variable ◽  
Daily Gain

This study was conducted to investigate the effects of weaning based upon different concentrate intake on growth performance, health, and antioxidant status of Southern Chinese Cattle. Thirty female calves were used in the trial. Weaning strategy was the primary variable of interest and treatments included weaning when a calf consumed 1000 (W1000), 750 (W750), or 500 (W500) g of starter for three consecutive days. All calves received colostrum within 4 h of birth and colostrum intake was monitored to ensure all calves consumed 1.5 L within 12 h of birth. Calves were then fed fresh milk up to 2 L/d for 7 days. Between d 7 and 13, calves were fed a liquid feed composed of 50% fresh milk, and 50% milk replacer (MR) at maximum rate of 3 L/d. For the remainder of the study, calves were provided 3 L/d MR. Concentrate (starter), and grass hay were available ad libitum starting on d 1. Milk, MR, starter, and hay intakes were recorded daily. Calf body weights and measurements were recorded at birth and every 4 wk until weaning. Average weaning ages were 48 ± 4.5, 58 ± 4.6, and 65 ± 4.8 for W500, W750, and W1000, respectively. Between wk 8 and wk 21, calves in the W500 and W750 treatments had higher (p < 0.05) starter intakes than calves in the W1000 treatment. Body height, body length, circumference of cannon bone, circumference of the chest did not differ with weaning strategy (p > 0.05). There was no difference in body weight (BW) and average daily gain (ADG) on wk 5 when any of the calves were weaned (p > 0.05). In wk 9, BW of calves in W1000 tended to be higher than that of W750 and W500 (p = 0.10). However, calves in W1000 lost their BW advantage in wk 13. Calves’ ADG was not different during the whole experiment period among treatments. Calves in W750 had higher plasma BHBA and the total antioxidant capacity which has been associated with a positive impact on health. Data in this experiment suggest that weaning when calves consume 750 g of starter can enhance ADG, DMI, feed efficiency, and selected blood parameters compared with weaning when calves consume 500 g of starter.

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