scholarly journals Effects of Phytonutrients on Ruminal Fermentation, Digestibility, and Microorganisms in Swamp Buffaloes

Animals ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 671
Author(s):  
Thiwakorn Ampapon ◽  
Kampanat Phesatcha ◽  
Metha Wanapat
Keyword(s):  
Methane Production ◽  
Volatile Fatty Acids ◽  
Latin Square ◽  
Rumen Fermentation ◽  
Neutral Detergent Fiber ◽  
Feed Consumption ◽  
Ruminal Fermentation ◽  
Swamp Buffaloes ◽  
Concentrate Mixture ◽  
Banana Flower

This experiment aimed to use dietary sources containing phytonutrients (PTN) such as mangosteen peel powder (MSP) and banana flower powder (BFP) as sources of phytonutrients. Four swamp buffalo bulls fitted with rumen fistulae were used as experimental animals. A digestion trial covering four periods was used according to a 4 × 4 Latin square design with four treatments: Treatment 1 (T1) = control (Cont), T2 = supplementation of PTN1 fed at 100 g/d, T3 = supplementation of PTN2 fed at 100 g/d, and T4 = supplementation of PTN3 fed at 100 g/d. The experiment was conducted for four periods; each period lasted for 21 days. All animals were fed a concentrate mixture at 0.5% body weight, while rice straw, water, and mineral blocks were fed ad libitum. The findings revealed significant increases in the digestibility of neutral detergent fiber (NDF) and acid detergent fiber (ADF), while no changes in dry matter feed consumption occurred due to PTN supplementation. Rumen fermentation end-products, such as total volatile fatty acids (TVFA), propionic acid (C3), and butyric acid (C4), were notably enhanced (p < 0.05) and there were the highest in PTN2 and PTN3, whilst acetic acid (C2) was significantly decreased with PTN supplementation groups. Furthermore, the rumen protozoal population was suppressed (p < 0.05), which resulted in decreased rumen methane production (p < 0.05), while the bacterial population was enhanced. Using PTN sources can improve rumen fermentation as well as mitigating rumen methane production.

scholarly journals Dietary rambutan peel powder as a rumen modifier in beef cattle

2020 ◽  
Vol 33 (5) ◽  
pp. 763-769
Author(s):  
Thiwakorn Ampapon ◽  
Metha Wanapat
Keyword(s):  
Beef Cattle ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Latin Square ◽  
Neutral Detergent Fiber ◽  
Napier Grass ◽  
Feed Consumption ◽  
Ruminal Fermentation ◽  
Fruit Peel ◽  
Negative Effect

Objective: The experiment was conducted to study the effect of rambutan (Nephelium lappaceum) fruit peel powder (RP) on feed consumption, digestibility of nutrients, ruminal fermentation dynamics and microbial population in Thai breed cattle.Methods: Four, 2-year old (250±15 kg) beef bull crossbreds (75% Brahman×25% local breed) were allotted to experimental treatments using a 4×4 Latin square design. Four dietary supplementation treatments were imposed; non-supplementation (control, T1); supplementation of RP fed at 2% of dry matter intake (DMI) (low, T2); supplementation of RP fed at 4% of DMI (medium, T3) and supplementation of RP fed at 6% of DMI (high, T4). All cattle were given a concentrate supplement at 1% of body weight while Napier grass was provided as a free choice.Results: The findings revealed that RP supplementation did not negatively affect (p>0.05) DMI of Napier grass, while RP intake and total DMI were the greatest in the RP supplementation at 4% and 6% DMI. Nevertheless, the nutrients (dry matter, organic matter, crude protein, neutral detergent fiber, and acid detergent fiber) digestibilities were not changed in the RP supplementation groups. Rumen fermentation parameters especially those of total volatile fatty acids, acetate and butyrate were not significantly changed. However, the propionate concentration was remarkably increased (p<0.05) in the RP supplementation. Notably, the ratio of acetate to propionate, the number of protozoa, as well as the methane estimation were significantly reduced in the RP supplemented groups (4% and 6% of DMI), while the counts of bacteria was not altered.Conclusion: Supplementation of RP (4% of DMI) improved rumen propionate production, reduced protozoal population and methane estimation (p<0.05) without a negative effect on feed consumption and nutrients total tract digestibilities in beef cattle. Using dietary rambutan fruit peel powder has potential promise as a rumen regulator.

scholarly journals Concentrate containing calcium salts of fatty acids rich in polyunsaturated fatty acids can change rumen fermentation in grazing goats

2018 ◽  
Vol 39 (6) ◽  
pp. 2621
Author(s):  
Ludmila Couto Gomes ◽  
Claudete Regina Alcalde ◽  
Julio Cesar Damasceno ◽  
Luiz Paulo Rigolon ◽  
Ana Paula Silva Possamai ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Latin Square ◽  
Rumen Fermentation ◽  
Ruminal Fermentation ◽  
Calcium Salts ◽  
Linear Effect ◽  
Concentrate Supplementation ◽  
Ruminal Ph

Feeding goats with calcium salts of fatty acids (CSFA) can supply ruminants with lipids, with minimal effects on ruminal fermentation and fiber digestibility. However, there is a shortage of information on the effect of CSFA on characteristics of rumen fermentation in grassland goats. Thus, the present study aimed to assess the addition of CSFA to concentrate on the parameters of rumen fermentation of grazing goats. Five rumen cannulated goats were distributed in a Latin square 5x5 design (treatments: 0%, 1.5%, 3.0%, 4.5% and 6.0% CSFA. The pH, ammonia N and volatile fatty acids (VFA) content were analyzed in the ruminal fluid at 0, 2, 4, 6 and 8 hours after concentrate supplementation. The pH and ammonia N concentration showed a linear effect with the addition of CSFA. There was no effect observed for the VFA molar concentration after grazing goats were fed with the experimental diet. In conclusion, further research is needed to investigate the addition of CSFA to goat diets because there is evidence that CSFA increases ruminal pH and decreases excess ruminal ammonia without changing the VFA concentration in the rumen fluid.

scholarly journals Using glycerin with chitosan extracted from shrimp residue to enhance rumen fermentation and feed use in native Thai bulls

2021 ◽  
pp. 1158-1164
Author(s):  
Anuthida Seankamsorn ◽  
Anusorn Cherdthong ◽  
Sarong So ◽  
Metha Wanapat
Keyword(s):  
Volatile Fatty Acids ◽  
Latin Square ◽  
Ammonia Nitrogen ◽  
Rumen Fermentation ◽  
Nutrient Digestibility ◽  
Crude Glycerin ◽  
Ruminal Fermentation ◽  
Design Factor ◽  
Ch4 Production ◽  
High Level

Background and Aim: Crude glycerin is changed to propionate in the rumen, while chitosan can be used as a feed supplement to increase propionic acid concentration and decrease methane (CH4) production. We hypothesized that supplementation with a combination of a high level of crude glycerin with chitosan could have a beneficial effect on ruminal fermentation and mitigate CH4 production. This study aimed to explore the combined effects of crude glycerin and chitosan supplementation on nutrient digestibility, rumen fermentation, and CH4 calculation in native Thai bulls. Materials and Methods: Four 2-year-old native Thai bulls, weighing 150±20 kg, were kept in a 2×2 factorial arrangement in a 4×4 Latin square design. Factor A represented the incorporation of crude glycerin at 10.5% and 21% of the dry matter (DM) of a total mixed ration (TMR), and factor B represented the supplementation of chitosan at 1% and 2% DM of a TMR. Results: Increasing levels of crude glycerin at 21% decreased DM intake by 0.62 kg/day compared with 10.5% crude glycerin (p<0.05), whereas nutrient digestibility did not change (p>0.05). The incorporated crude glycerin and supplemented chitosan levels did not affect the pH, temperature, concentrations of ammonia-nitrogen, microbial population, and blood urea nitrogen (p>0.05). Supplemented chitosan and incorporated crude glycerin did not show any interaction effects on the molar portions and total volatile fatty acids (VFAs), except estimated CH4. Increasing the incorporated crude glycerin levels increased propionate and decreased the ratio of acetate to propionate ratio, whereas levels of butyrate, acetate, and total VFAs were unchanged. The combination of crude glycerin at 21% in the TMR with chitosan at 2% reduced CH4 estimation by 5.08% compared with the other feed treatment. Conclusion: Increasing incorporated crude glycerin levels in a TMR significantly elevated the propionate concentration, whereas combining 21% crude glycerin in the TMR diet with 2% chitosan supplementation could depress CH4 estimation more effectively than adding one of these supplements alone.

scholarly journals In Vitro Fermentation Characteristics and Methane Mitigation Responded to Flavonoid Extract Levels from Alternanthera sissoo and Dietary Ratios

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 109
Author(s):  
Sukruthai Sommai ◽  
Anusorn Cherdthong ◽  
Chanon Suntara ◽  
Sarong So ◽  
Metha Wanapat ◽  
...  
Keyword(s):  
Methane Production ◽  
Crude Protein ◽  
Volatile Fatty Acids ◽  
Gas Production ◽  
Neutral Detergent Fiber ◽  
Ruminal Fermentation ◽  
Acid Detergent Fiber ◽  
In Vitro Fermentation

Two experiments were conducted under this study: Experiment 1 was to study production yield, chemical composition, and in vitro degradability of Brazilian spinach (Alternanthera sissoo; BS) leaf and leaf + leaf-stalk at various maturity ages of 15, 30, 45, and 60 days after plantation and regrowth and Experiment 2 was to evaluate the effect of flavonoid extract from BS leaf and leaf + leaf-stalk and dietary ratios on ruminal gas production, fermentation characteristics, and in vitro degradability. Experiment 1 showed that maturity ages after planting and regrowth increased, the yield significantly increased. Increasing maturity ages significantly (p < 0.05) increased neutral detergent fiber and acid detergent fiber content and decreased crude protein content, total flavonoid (TF) content, and degradability for both leaf and leaf + leaf-stalk. Maturity ages from 15 to 30 days after plantation and regrowth resulted (p < 0.05) the highest TF content and degradability for both leaf and leaf + leaf-stalk. Thus, BS leaf and leaf + leaf-stalk samples from 15 to 30 days of age were used for flavonoid extraction and used in the Experiment 2. Experiment 2 was conducted according to a 3 × 5 factorial experiment. Three roughage to concentrate (R:C) ratios at 50:50, 40:60, and 30:70 were used, and five levels of flavonoid extract (FE) at 0, 10, 20, 30, and 40 mg of substrate dry matter (DM) were supplemented. Experiment 2 showed that R:C ratio and FE had an interaction effect only on acetate to propionate ratio. Varying R:C ratios significantly increased (p < 0.05) in vitro DM degradability, total volatile fatty acids (VFA), and propionate (C3) concentration. FE supplementation linearly (p < 0.05) increased total VFA and C3 concentration and decreased methane production and protozoal population. This study could conclude that FE from BS could effectively modulate ruminal fermentation and decrease methane production. However, in vivo study needs to elucidate in order to validate the present results.

scholarly journals Evaluation of ensiled soy sauce by-product combined with several additives as an animal feed

2020 ◽  
Vol 13 (5) ◽  
pp. 940-946
Author(s):  
Sadarman Sadarman ◽  
Muhammad Ridla ◽  
Nahrowi Nahrowi ◽  
Roni Ridwan ◽  
Anuraga Jayanegara
Keyword(s):  
Chemical Composition ◽  
Propionic Acid ◽  
Methane Production ◽  
Volatile Fatty Acids ◽  
Animal Feed ◽  
Rumen Fermentation ◽  
Soy Sauce ◽  
Ruminal Fermentation ◽  
In Vitro Rumen Fermentation

Aim: The present experiment aimed to evaluate the use of different additives, i.e., lactic acid bacteria (LAB) inoculant, tannin extract, and propionic acid, on the chemical composition, fermentative characteristics, and in vitro ruminal fermentation of soy sauce by-product (SSB) silage. Materials and Methods: SSB was subjected to seven silage additive treatments: Fresh SSB, ensiled SSB, ensiled SSB+LAB, ensiled SSB+2% acacia tannin, ensiled SSB+2% chestnut tannin, ensiled SSB+0.5% propionic acid, and ensiled SSB+1% acacia tannin+1% chestnut tannin+0.5% propionic acid. Ensiling was performed for 30 days in three replicates, and each replicate was made in duplicate. The samples were evaluated for their chemical composition and silage fermentation characteristics and were tested in an in vitro rumen fermentation system. Results: In general, the nutrient compositions did not differ among the tested SSBs in response to the different additives used. The addition of tannins, either acacia or chestnut, and propionic acid significantly decreased the pH of the ensiled SSB (p<0.05). The addition of several additives (except LAB) decreased the ammonia concentration in SSB silage (p<0.05). The total volatile fatty acids in the in vitro rumen fermentation profile of the ensiled SSB were not significantly altered by the various additives applied. The addition of some additives, i.e., ensiled SSB+LAB and ensiled SSB+2% acacia tannin, reduced the digestibility values of the SSB (p<0.05). Different silage additives did not significantly affect methane production, although the addition of acacia tannins tended to result in the lowest methane production among treatments. Conclusion: The use of additives, particularly 2% acacia tannins, can reduce proteolysis in SSB silage.

Influence of amount and frequency of protein supplementation to steers consuming low-quality, cool-season forage: Intake, nutrient digestibility, and ruminal fermentation

2021 ◽  
Author(s):  
Bruno I Cappellozza ◽  
David W Bohnert ◽  
Maria M Reis ◽  
Kendall C Swanson ◽  
Stephanie J Falck ◽  
...  
Keyword(s):  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Latin Square ◽  
Nutrient Digestibility ◽  
Protein Supplementation ◽  
Neutral Detergent Fiber ◽  
Management Tool ◽  
Ruminal Fermentation ◽  
Cool Season ◽  
Linear Effect

Abstract This experiment evaluated the influence of protein supplementation frequency (SF) and amount offered on intake, nutrient digestibility, and ruminal fermentation by rumen-fistulated beef steers consuming low-quality [2.9% crude protein (CP); dry matter (DM) basis], cool-season forage. Seven Angus × Hereford steers (300 ± 27 kg) fitted with ruminal cannulas were randomly assigned to 1 of 7 treatments in an incomplete 7 × 4 Latin square. Treatments, in a 2 × 3 factorial design plus a non-supplemented control (CON), consisted of 2 levels of supplemental soybean meal, 100% (F) or 50% (H) of the estimated rumen-degradable protein requirement, provided daily (D), once every 5 d (5D), or once every 10 d (10D). Experimental periods were 30 d and dry matter intake (DMI) was measured from d 19 to 28. On d 21 (all supplements provided) and 30 (only daily supplements provided; day immediately prior to supplementation for 5D and 10D treatments) ruminal fluid was collected for ruminal pH, ammonia-N (NH3), volatile fatty acids (VFA), and determination of ruminal fermentation variables. Forage and total DM, organic matter (OM), and nitrogen (N) intake increased with supplementation (P ≤ 0.04). However, a linear effect of SF × amount of supplement interaction was observed for forage and total DM, OM, and N intake (P ≤ 0.04), with each variable decreasing as SF decreased, but the decrease being greater with F vs. H. Apparent total tract DM, OM, and neutral detergent fiber (NDF) digestibility was not affected by supplementation or amount of supplement provided (P ≥ 0.10). In contrast, N digestibility increased with supplementation and for F vs. H (P &lt; 0.01). Digestibility of DM, OM, and N increased linearly as SF decreased (P ≤ 0.03). When all supplements were provided, ruminal NH3, total VFA, and molar proportions of all individual VFA increased with supplementation (P ≤ 0.04), whereas acetate:propionate ratio decreased (P &lt; 0.01). When only daily supplements were provided, none of the aforementioned fermentation parameters were affected (P ≥ 0.09). In summary, reducing the amount of supplemental CP provided to ruminants consuming low-quality forages, when supplementation intervals are greater than 5 d, can be a management tool to maintain acceptable levels of DMI, nutrient digestibility, and ruminal fermentation while reducing supplementation cost.

scholarly journals COMPLETE RUMEN MODIFIER SUPPLEMENTATION IN CORN COB SILAGE BASAL DIET OF LAMB REDUCES METHANE EMISSION

2017 ◽  
Vol 18 (1) ◽  
pp. 33
Author(s):  
Dwi Yulistiani ◽  
Wisri Puastuti ◽  
Budi Haryanto ◽  
Agung Purnomoadi ◽  
M. Kurihara ◽  
...  
Keyword(s):  
Methane Emission ◽  
Methane Production ◽  
Basal Diet ◽  
Rumen Fermentation ◽  
Nutrient Digestibility ◽  
The Body ◽  
Neutral Detergent Fiber ◽  
Feed Consumption ◽  
Feed Additive ◽  
Corn Cob

<p>Feeding animal with fibrous materials such as corn cob will emit methane. Complete rumen modifier (CRM) is an improved feed additive comprised a mixture of Sapindus rarak, sesbania, albizia leaves and minerals that functions as a methane inhibitor. The study aimed to determine the effect of CRM supplementation on the feed intake, nutrient digestibility, rumen fermentation, methane emission and growth of lambs. The experiment was designed in a complete randomized block, four levels of CRM (0%, 1%, 2%, and 3%), six group of 24 male lambs per treatment based on the body weight.  Basal diet used was corn cob silage ad libitum and concentrate (500 g/day) as a supplement. The results showed that CRM supplementation did not affect feed consumption and average daily gain, but significantly decreased the dry matter, as well as organic matter and protein digestibility.  The neutral detergent fiber (NDF) and acid detergent fiber (ADF) digestibility linearly decreased with increasing level of CRM. Ruminal pH, ammonia concentration and volatile fatty acid (VFA) concentration were not affected by the CRM supplementation. Methane production expressed in kJ/MJ gross energy (GE) or digestible energy (DE) intake significantly decreased by 32% at the 2–3% CRM supplementation and reduced by 39% when methane production was expressed in g/kg digested NDF. It can be concluded that 2% CRM supplementation in the corn cob basal diet did not affect nutrient intake and growth rate of the lamb, as well as rumen fermentation.  The study suggests that CRM is an environmentally friendly feed additive for lamb</p>

scholarly journals Rumen antimethanogenic effect ofSaponaria officinalisL. phytochemicalsin vitro

2014 ◽  
Vol 152 (6) ◽  
pp. 981-993 ◽  
Author(s):  
A. CIESLAK ◽  
P. ZMORA ◽  
A. STOCHMAL ◽  
L. PECIO ◽  
W. OLESZEK ◽  
...  
Keyword(s):  
Methane Production ◽  
Volatile Fatty Acids ◽  
Microbial Population ◽  
Secondary Compounds ◽  
Rumen Fermentation ◽  
Triterpenoid Saponin ◽  
Dependent Manner ◽  
Ruminal Fermentation ◽  
Rumen Microorganisms

SUMMARYAlthough the effect of saponins or saponin-containing plants on rumen microorganisms and rumen fermentation has been intensively investigated, this issue still requires special attention. Many of the phenomena occurring in the rumen related to dietary saponin supplementation are still not fully understood.Saponaria officinalisis a triterpenoid saponin-containing plant; thus, the aim of the present study was to evaluate the effect ofS. officinalisL. powdered root, methanolic extract of theS. officinalisroot (SOR) and the effect of the separated fractions (polysaccharides, saponins and phenolics) ofS. officinalison rumen methanogenesis, microbial population and rumen fermentation characteristics in anin vitrobatch culture fermentation system. The powdered root (raw plant material) andS. officinalisextract (SOE) decreasedin vitromethane production and consequently reduced the microbial population in a dose-dependent manner. The inhibition of methanogenesis was accompanied by changes in the volatile fatty acids profile.In vitrodry matter digestibility was not affected by any of the secondary compounds applied. The highest applied doses of SOE caused a higher reduction in methanogenesis (33·5v. 14·4%) than the highest doses of powdered root form. Such results suggest that the basic components of the SOR could interact with phytochemicals or that the phytochemicals became physically less available for microbiota, resulting in a decreased antimethanogenic activity of the powdered rootv. the extract. Among all the fractions selected, the saponin fraction exerted the greatest impact on ruminal fermentation. In conclusion, saponins decreased methane production by 29% in comparison with the control. This decrease was related to the reduction in protozoa and methanogen counts. It is proposed thatS. officinalishas the potential to inhibit rumen methanogenesis without affecting rumen fermentation adversely.

scholarly journals Fecal excretion patterns and short term bias of internal and external markers in a digestion assay with cattle

2011 ◽  
Vol 40 (3) ◽  
pp. 657-665 ◽  
Author(s):  
Cláudia Batista Sampaio ◽  
Edenio Detmann ◽  
Tiago Neves Pereira Valente ◽  
Viviane Aparecida Carli Costa ◽  
Sebastião de Campos Valadares Filho ◽  
...  
Keyword(s):  
Titanium Dioxide ◽  
Latin Square ◽  
Chromic Oxide ◽  
Neutral Detergent Fiber ◽  
Fecal Excretion ◽  
Short Term ◽  
Concentrate Mixture ◽  
Red Angus ◽  
Internal Markers ◽  
Fecal Sampling

The objective of this work was to evaluate the fecal excretion profile and the short term bias of the external markers chromic oxide and titanium dioxide and the internal markers indigestible dry matter (iDM), indigestible neutral detergent fiber (iNDF) and indigestible acid detergent fiber (iADF) in a digestion trial with cattle fed different diets. Fourteen F1 Red Angus × Nellore bulls averaging 12 months old and 287 kg were kept in individual stalls. The animals were fed elephant grass silage, corn silage or signal grass hay, supplemented or not with 20% of concentrate mixture. The experiment consisted of two 13-day experimental periods according to a 2 × 2 Latin square design with seven squares grouping. The animals received 10 g of chromic oxide and 10 g of titanium dioxide through esophageal sounder daily. Fecal grab samples were obtained directly from the rectum of the animals at 1:30 a.m., 3:00 a.m., 4:30 a.m., 6:00 a.m., 7:30 a.m., 9:00 a.m., 10:30 a.m., 12:00 p.m., 1:30 p.m., 3:00 p.m., 4:30 p.m., 6:00 p.m., 7:30 p.m., 9:00 p.m., 10:30 p.m. and 12:00 a.m. There was no effect of forages or concentrate levels on the dimension of excretory cycle. The fundamental period of excretory cycle was 15.06 and 18.66 hours for chromic oxide and titanium dioxide, respectively and 9.93, 9.29 and 10.55 hours for iDM, iNDF and iADF, respectively. The oscillation range was higher for external markers. Considering the characteristics of all evaluated markers together, it is recommended fecal sampling designs with at least four collections, distributed during the day or only in the diurnal period for obtaining fecal excretion estimates free of short term bias.

scholarly journals Pomegranate seed oil rich in conjugated linolenic acids reduces in vitro methane production

1970 ◽  
Vol 46 (3) ◽  
pp. 325-335
Author(s):  
E. Maleki ◽  
G.Y. Meng ◽  
M. Faseleh Jahromi ◽  
R. Jorfi ◽  
A. Khoddami ◽  
...  
Keyword(s):  
Methane Production ◽  
Volatile Fatty Acids ◽  
Seed Oil ◽  
Control Diet ◽  
Nutrient Utilization ◽  
Gas Production ◽  
Metabolizable Energy ◽  
Ruminal Fermentation ◽  
Ch4 Production

The objective of this study was to determine the effect of pomegranate (Punica granatum L.) seed oil (PSO) on gas and methane (CH4) production, ruminal fermentation and microbial populations under in vitro conditions. Three treatments consisting of a control diet containing 10 mg tallow (CON); the control diet with 5 mg PSO + 5 mg tallow (MPSO) and the control diet containing 10 mg PSO (HPSO) were compared. Ten mg of the experimental fat/oil samples were inserted into a gas-tight 100 mL plastic syringe containing 30 mL of an incubation inoculum and 250 mg of a basic substrate of a hay/concentrate (1/1, w/w) mixture. In vitro gas production was recorded over 0, 2, 4, 6, 8, 10, 12 and 24 h of incubation. After 24 hours, incubation was stopped, and methane production, pH, volatile fatty acids (VFAs) and microbial counts were measured in the inoculant. Gas production at 4, 6, 8, 10, 12 and 24 h incubation, metabolizable energy and in vitro organic matter disappearance increased linearly and quadratically as level of PSO increased. Furthermore, the 10 mg PSO (HPSO) decreased CH4 production by 21.0% compared with the control (CON) group. There were no significant differences in total and individual VFA concentrations between different levels of PSO, except for butyric acid. After 24 h of incubation, methanogenesis decreased in the HPSO compared with the MPSO and CON treatments. In addition, total bacteria and protozoa counts increased with rising PSO levels, while population methanogenesis declined significantly. These results suggested that PSO could reduce methane emissions, which might be beneficial to nutrient utilization and growth in ruminants.

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