scholarly journals Ameliorative Effect of Bacillus subtilis on Growth Performance and Intestinal Architecture in Broiler Infected with Salmonella

Animals ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 190 ◽  
Author(s):  
Alaeldein M. Abudabos ◽  
Muttahar H. Ali ◽  
Mohammed A. Nassan ◽  
Ahmad A. Saleh
Keyword(s):  
Bacillus Subtilis ◽  
Basal Diet ◽  
Positive Control ◽  
Negative Control ◽  
Broiler Chicks ◽  
Feed Conversion ◽  
Negative Effects ◽  
Villus Height ◽  
Bacillus Subtilis Atcc ◽  
Ameliorative Effect

A total of 600 day-old broiler chicks (Ross 308) confirmed for the absence of Salmonella were randomly allocated to five treatments each with 10 replicates: negative control (basal diet only); positive control (basal diet) + infected with Salmonella; T1, Salmonella infected + avilamycin; T2, Salmonella infected + Bacillus subtilis (ATCC PTA-6737; 2 × 107 CFU/g) and T3, Salmonella infected + B. subtilis (DSM 172999; 1.2 × 106 CFU/g). The results revealed that feed intake (FI) and body weight (BW) were significantly (p < 0.01) lower in T1 compared to T2. The feed conversion ratio (FCR) was significantly (p < 0.01) lower in T2 and T3 compared to other treatments. Similarly, the performance efficiency factor (PEF) was also significantly (p < 0.01) higher in T2 and T3 compared to positive control. Villus height was significantly (p < 0.01) higher in T2 compared to all other treatments. However, villus width and surface area were significantly (p < 0.01) higher in T1. In conclusion, dietary supplementation with B. subtilis improved growth and intestinal health by reversing the negative effects of Salmonellosis.

scholarly journals Growth Performance, Serum Biochemical Indices, Duodenal Histomorphology, and Cecal Microbiota of Broiler Chickens Fed on Diets Supplemented with Cinnamon Bark Powder at Prestarter and Starter Phases

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 94
Author(s):  
Mohammed M. Qaid ◽  
Saud I. Al-Mufarrej ◽  
Mahmoud M. Azzam ◽  
Maged A. Al-Garadi ◽  
Hani H. Albaadani ◽  
...  
Keyword(s):  
Body Weight ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
Basal Diet ◽  
Positive Control ◽  
Negative Control ◽  
Broiler Chicks ◽  
Feed Conversion ◽  
Serum Globulin ◽  
Maximum Improvement

Ross 308 broiler chicks (n = 240) aged 1 day were assigned to five groups for eight replicates (six chicks for each) (3♂ and 3♀). Basal dietary groups were supplemented by 2000, 4000, and 6000 mg/kg cinnamon (CN) for 21 days. Basal diet alone was used as a negative control, and basal antibiotic diet (Colimox) was used as a positive control. At 10, 14, and 21 days of age, chicks that received 2000 mg CN and Colimox had a higher body weight, resulting in an increase in body weight gain. CN also resulted in the maximum improvement in the feed conversion ratio and feed efficiency over 1–21 days at the level of 2000 mg/kg. At days 10, the maximum relative breast weight was 2000 mg/kg of CN. Mean serum albumin concentrations, duodenal villus height, and goblet cell density increased (p < 0.05) by 2000 mg/kg of CN, and mean serum globulin and total protein concentrations and crypt depth increased (p < 0.05) by 6000 mg/kg of CN compared with control. Increased cecal Escherichia coli number was CN dose-dependent. In conclusion, dietary inclusion of 2000 mg/kg CN can be applied as an alternative to in-feed antibiotics for broiler starter diet.

scholarly journals Effect of Spearmint (Mentha spicata) on Performance and Blood Serum Parameter of Broiler

2015 ◽  
Vol 8 (2) ◽  
pp. 1600-1606
Author(s):  
Huda Mohammed Saad Elamin ◽  
K.A. Mohamed ◽  
Mukhtar A. Mukhtar
Keyword(s):  
Body Weight Gain ◽  
Basal Diet ◽  
Positive Control ◽  
Negative Control ◽  
Feed Consumption ◽  
Broiler Chicks ◽  
Growth Promoter ◽  
Feed Conversion ◽  
Mentha Spicata ◽  
Dietary Treatments

The present study was planned to evaluate the response of broiler chicks to diets containing graded levels of spearmint (Mentha spicata).  A total of 200 , five days-old, unsexes (cob) broiler chicks were used. Chicks were weighed individually and assigned randomly to five groups of 40 chicks per treatment and each treatment was subdivided to four replicates of 10 chicks each. Five experimental diets were formulated (A, B, C, D and E) to meet the nutrient requirements of broiler chicks. The experimental parameters examined were growth performance, serum metabolite, electrolyte and enzyme activities and economic appreciable. Diet (A) Negative      Control (NC), a basal diet without antibiotic or spearmint,  diet B as Positive Control (PC) basal diet supplemented with antibiotic (Neomycin 16 mg/Kg), diets C,D and E were basal diet  supplemented with spearmint at levels of 1,1.5,and 2%, respectively. Result obtained showed that addition of spearmint improved significantly (P<0.05) the performance (body weight gain and feed conversion ratio) of broiler chicks compared to group fed on NC while no significant (P<0.05) differences between chicks fed on diets supplemented with spearmint  and PC group observed, no significantly difference was found in feed consumption between all treated groups. The mortality rate did not influenced by the dietary treatments. Results revealed no significant differences (P<0.05) observed among all treatment groups in the carcass dressing percentages, Supplementation of broiler diets with spearmint at various levels was resulted in significant (p>0.05) reduction of abdominal fat, serum cholesterol, and AST enzyme activity compared to both NC and PC groups while the levels of urea, total protein, glucose and ALP and serum electrolytes levels remained unchanged. The highest profitability ratio (1.13) was obtained from the diet (E) supplemented with 2% spearmint. This study showed that spearmint when added as growth promoter in broiler diets has a similar effect on performance as that of antibiotic without any adverse effects.

scholarly journals Response of broiler chicks to L-Glutamine feeding in the immediate pre- and post-hatch periods

2021 ◽  
Vol 50 (6) ◽  
Author(s):  
S. Karamik ◽  
C. Kop-Bozbay
Keyword(s):  
Digestive System ◽  
Muscle Development ◽  
System Development ◽  
Positive Control ◽  
Negative Control ◽  
Intestinal Morphology ◽  
Broiler Chicks ◽  
Villus Height ◽  
In Ovo ◽  
Small Intestinal

This study was conducted to investigate the effects of in ovo and post-hatching supplementation with L-Glutamine (Gln) on hatching characteristics, performance, small intestinal morphology, and muscle development of broilers. At day 18 of incubation, 960 fertilized eggs were allocated to four treatments with six replicates. Eggs were i) not injected (negative control) (NC), ii) subject to the standard incubation procedure (PG), iii) injected with 1 ml sterile solution with 0.9% salt (positive control) (PC), and iv) injected with 1% Gln solution (IG). On hatching, the SC chicks were fed with 1% Gln for seven days. The remaining chicks were fed a commercial starter feed. After hatching, there were six replicates of 28 birds in each treatment. Hatchability and yolk sac weight were lower and yolk-free chick weight (YFCW), whole gastrointestinal tract (GIT) and breast muscle weights were higher at hatching for chicks from the IG treatment. At 42 days old, feed conversation ratio (FCR) was lower in birds that had Gln added to their diet than for the other treatments. The FCR was also lower in IG birds than birds in the NC group. The GIT weight, villus height, villus width and crypt depth of the birds receiving dietary supplementation of Gln were greater than those of birds in PC and IG. Thus, in ovo injection of Gln improved hatching characteristics except for hatchability. Further, in ovo and dietary Gln administration reduced FCR by stimulating digestive system development.

scholarly journals Cholesterol-lowering activity of lactic acid bacteria and yeast when used as probiotics in laying quail (Coturnix coturnix Japonica)

2020 ◽  
Vol 45 (4) ◽  
pp. 305-319
Author(s):  
L. Istiqomah ◽  
A. A. Sakti ◽  
A. Sofyan ◽  
H. Herdian ◽  
A. S. Anggraeni
Keyword(s):  
Density Lipoprotein ◽  
Basal Diet ◽  
Positive Control ◽  
Negative Control ◽  
Production Performance ◽  
Coturnix Japonica ◽  
Coturnix Coturnix Japonica ◽  
Feed Conversion ◽  
Coturnix Coturnix ◽  
Laying Quail

This study aimed to evaluate the administration of Lactobacillus plantarum AKK-30 and Saccharomyces cerevisiae B-18 as probiotics in reducing cholesterol of blood, egg, and meat and also the production performance of laying quail (Coturnix coturnix Japonica). A number of 600 birds of the twenty one-day-old quails were distributed in a completely randomized design with 5 treatments and 6 replications (initial BW= 101.35 ± 1.64 g). Treatments were as follows: A = negative control (basal diet without probiotic), B = 1% of L. plantarum AKK-30 (107 cfu/g), C = 1% of S. cerevisiae B-18 (106 cfu/g), D = 1% of a probiotic consortium, E = positive control (commercial probiotic). Parameters observed were blood biochemistry profiles, product quality, and production performance. The results revealed that administration of 1% S. cerevisiae (C) resulted the lowest cholesterol in blood (101.75 mg/dL) and egg (9.44 mg/g) and while administration of 1% L. plantarum(B) increased meat protein and decreased fat content (P<0.05). Level of blood triglycerides, low-density lipoprotein (LDL), and high-density lipoprotein (HDL) were not different among treatments. Probiotic treatments did not affect the growth performance, quail day production (QDP), feed conversion ratio (FCR) and egg uniformity. However, probiotic increased diameter of egg yolk (P<0.05). It was concluded that administration of single probiotic L. plantarum AKK-30 or S. cerevisiae B-18 improves the quality of laying quail products. 

scholarly journals Effect of Antibiotic, Phytobiotic and Probiotic Supplementation on Growth, Blood Indices and Intestine Health in Broiler Chicks Challenged with Clostridium perfringens

Animals ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 507 ◽  
Author(s):  
Elsayed O. S. Hussein ◽  
Shamseldein H. Ahmed ◽  
Alaeldein M. Abudabos ◽  
Mashael R. Aljumaah ◽  
Manal M. Alkhlulaifi ◽  
...  
Keyword(s):  
Clostridium Perfringens ◽  
Basal Diet ◽  
Negative Control ◽  
Feed Additives ◽  
Broiler Chicks ◽  
Feed Conversion ◽  
Blood Indices ◽  
Blood Constituents ◽  
Glucose Levels ◽  
Notable Increase

This study evaluated the effects of feed additives on the growth, blood biochemistry and intestinal health of broiler chicks. A total of 378 of broiler chicks (Ross 308) were randomly allotted to seven groups. Chicks were fed a basal diet with 0.0 (control negative), 0.0 (control positive), 0.1, 0.5, 0.12, 0.5 + 0.12 and 0.2 g Kg−1 of Maxus, CloStat, Sangrovit Extra, CloStat + Sangrovit Extra and Gallipro Tech, respectively for 35 days. After 15 days, the chicks were inoculated with Clostridium perfringens. All feed additives were found to enhance growth performance and feed efficiency. The best feed conversion ratio was found in the Negative Control, CloStat + Sangrovit Extra and Gallipro Tect groups, respectively. A notable increase in villus length, total villus area, small intestine weight, ilium weight and total lesion score was found in chicks supplemented with Bacillus subtilis. Besides, the dietary inclusion of phytobiotic compounds showed potential in reducing the serum Alanine aminotransferase (ALT) concentration and increasing the glucose levels. All intestine and liver histopathological signs were reduced in chicks fed a probiotic-supplemented diet. Our findings indicate that supplementation with probiotics and phytobiotics alone or in combined form can be used to enhance performance, intestine health and blood constituents against C. perfringens infection in broiler chicks.

scholarly journals Dietary supplementation of Neem (Azadirachta indica) leaf extracts improved growth performance and reduced production cost in broilers

2020 ◽  
Vol 13 (6) ◽  
pp. 1050-1055
Author(s):  
Torun Kumar Paul ◽  
Md. Mehedi Hasan ◽  
Md. Anowarul Haque ◽  
Sudipta Talukder ◽  
Yousuf Ali Sarker ◽  
...  
Keyword(s):  
Body Weight ◽  
Azadirachta Indica ◽  
Basal Diet ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Broiler Chicks ◽  
Growth Promoter ◽  
Leaf Extracts ◽  
Neem Leaves

Background and Aim: Global trend to remove the antibiotic growth promoter (AGP) from animals contributes to the exploration of successful measures to sustain production and reduces the intestinal diseases in the post-AGP era. Plant extracts, therefore, have been used to improve performance and intestinal health. Here, we conducted a study to evaluate the effects of neem (Azadirachta indica) leaf extracts (NLE) as alternatives to AGPs in broiler chickens. Materials and Methods: Sixty day-old broiler chicks were assigned to 12-floor pens, each stocked with five birds and divided into three treatment groups of four pens per treatment. The groups were: Negative control, basal diet without additives; positive control, basal diet with antibiotics and vitamins; and NLE treated group, basal diet supplemented with 0.1% aqua extract of neem leaves. Results: Overall feed intake was significantly (p≤0.05) highest in the positive control. Higher body weight, higher dressing percentage, and lower feed conversion ratio were observed in birds treated with NLE compared to the negative control group (p≤0.05) but not the positive control group (p>0.05). There was no significant variation in hematology between different groups. Furthermore, the economic evaluation indicated that the NLE treatment was found cheaper than control and antibiotic treatment in cost-benefit analysis. Conclusion: We suggest NLE might be a cheaper alternative to antibiotics in broiler production as indicated by improved body weight and feed efficiency.

scholarly journals Phytogenic additive as an alternative to growth promoters in broiler chickens

2009 ◽  
Vol 39 (2) ◽  
pp. 522-527 ◽  
Author(s):  
Gerson Neudí Scheuermann ◽  
Anildo Cunha Junior ◽  
Lucas Cypriano ◽  
Alexandre Mossate Gabbi
Keyword(s):  
Body Weight ◽  
Broiler Chickens ◽  
Positive Control ◽  
Negative Control ◽  
Feed Additive ◽  
Broiler Chicks ◽  
Growth Promoter ◽  
Feed Conversion ◽  
Growth Promoters ◽  
The Cost

This study evaluated a phytogenic feed additive for broiler chickens. A total of 1,632 broiler chicks were distributed into four treatments: negative control (without growth promoter); positive control (avilamycine, 10ppm + colistin, 15ppm); and two alternative treatments with 150ppm of phytogenic additive, one with a reduced Ca and P levels diet (PA-R1) and the other with lower energy, and amino acids, besides Ca and P (PA-R2). The trial was conducted with 12 replicates, each consisted of a pen with 34 birds. The alternative diets showed body weight intermediate to the two controls at 42 days, with no significant (P>0.05) treatment effect on feed conversion ratio. No treatment differences (P>0.05) on carcass yield and composition was observed. There was a tendency of abdominal fat lipids saturation, when the phytogenic additive was used, as possible consequence of a decreased level of soybean oil in the diets. A difference (P<0.001) on ingredient consumption profile was observed between the treatments. All together, this study showed a possibility to reduce the cost of total feed used to produce a broilers or a ton of body weight by the utilization of the tested phytogenic additive.

scholarly journals Preparing of saccharomyces cereviciae Synbiotic for Reducing Expermental Infection of salmonella typhimurium in Broiler 2–advanced age ( 16 -30 days)

2008 ◽  
Vol 32 (1) ◽  
pp. 47-58
Author(s):  
Ghada A. Qatan
Keyword(s):  
Salmonella Typhimurium ◽  
Experimental Infection ◽  
Physiological Stress ◽  
Blood Parameters ◽  
Positive Control ◽  
Negative Control ◽  
Broiler Chicks ◽  
Feed Conversion ◽  
Increase Body Weight ◽  
Mannan Oligosaccharide

This study aimed to prepare a synbiotic of Sacch. cerevisiae and used as 0.1 and 0.2 % in feed (T2,T3) for reducing the experimental infection of Salmonella typhimurium to broiler chicks ( -6103days) by using 40 birds divided into four treatments , T1 was negative control with 0.1 % synbiotics and T4 was positive control with experimental infection only , production and blood parameters were studied , and the results showed the following: A synbiotic of Sacch. cerevisiae was made which have 10 7 cfu of Sacch. cerevisiae and 10 mg Mannan Oligosaccharide per gram .Using 0.1 and 0.2 % of Sacch. cerevisiae synbiotic reduced experimental infection of Salmonella typhimurium via increase body weight and feed conversion and decrease mortality and physiological stress of experimental infection.

scholarly journals EFEITO DA ADIÇÃO DE GENGIBRE (Zingiber officinale) EM PÓ SOBRE O DESEMPENHO DE FRANGOS DE CORTE NA FASE INICIAL

2017 ◽  
Vol 13 (Especial 2) ◽  
pp. 156-163
Author(s):  
Vilson Matias Pinto ◽  
Paulo Ricardo de Sá da Costa Leite ◽  
Mônica Maria de Almeida Brainer ◽  
Brena Cristine Rosário Silva ◽  
Leidyanne Guimarães Silva
Keyword(s):  
Zingiber Officinale ◽  
Positive Control ◽  
Negative Control ◽  
Production Performance ◽  
Broiler Chicks ◽  
Growth Promoter ◽  
Feed Conversion ◽  
Growth Promoters ◽  
Performance Variables ◽  
Randomized Design

The objective of this study was to evaluate the production performance of broilers with the addition of ginger (Zingiber officinale) in broiler diets in starter phases. Were used 630 broiler chicks in a completely randomized design with five treatments and seven repetitions with eighteen birds each. The treatments consist of a reference diet with corn and soybean meal and adding different levels of powdered ginger, five treatments, consisting of positive control, negative control and three levels of ginger (0.50%, 0.75%; 1.0%). Were evaluated at the end of each phase, feed intake, weight gain, feed conversion, body weight, pancreatic weight and length and weight of the intestine. The data will be submitted to analysis of variance and means to regression analysis with 5% significance. No effects of the treatments on the performance variables were observed in the initial stage of creation. With this result, at twenty-one days old, we observed the potential for substitution of growth promoters for powdered ginger. The inclusion of up to 1% of powdered ginger did not affect bird performance providing results similar to positive control treatments with growth promoter.

scholarly journals Comparison of Ameliorative Effects between Probiotic and Biodegradable Bacillus subtilis on Zearalenone Toxicosis in Gilts

Toxins ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 882
Author(s):  
Wenqiang Shen ◽  
Yaojun Liu ◽  
Xinyue Zhang ◽  
Xiong Zhang ◽  
Xiaoping Rong ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Pathogenic Bacteria ◽  
Control Diet ◽  
Hormone Secretion ◽  
Basal Diet ◽  
Diet Group ◽  
Conversion Ratio ◽  
Feed Conversion ◽  
Negative Effects ◽  
Agent Group

This study was conducted to compare the potential ameliorative effects between probiotic Bacillus subtilis and biodegradable Bacillus subtilis on zearalenone (ZEN) toxicosis in gilts. Thirty-six Landrace×Yorkshire gilts (average BW = 64 kg) were randomly divided into four groups: (1) Normal control diet group (NC) fed the basal diet containing few ZEN (17.5 μg/kg); (2) ZEN contaminated group (ZC) fed the contaminated diet containing an exceeded limit dose of ZEN (about 300 μg/kg); (3) Probiotic agent group (PB) fed the ZC diet with added 5 × 109 CFU/kg of probiotic Bacillus subtilis ANSB010; (4) Biodegradable agent group (DA) fed the ZC diet with added 5 × 109 CFU/kg of biodegradable Bacillus subtilis ANSB01G. Results showed that Bacillus subtilis ANSB010 and ANSB01G isolated from broiler intestinal chyme had similar inhibitory activities against common pathogenic bacteria. In addition, the feed conversion ratio and the vulva size in DA group were significantly lower than ZC group (p < 0.05). The levels of IgG, IgM, IL-2 and TNFα in the ZC group were significantly higher than PB and DA groups (p < 0.05). The levels of estradiol and prolactin in the ZC group was significantly higher than those of the NC and DA groups (p < 0.05). Additionally, the residual ZEN in the feces of the ZC and PB groups were higher than those of the NC and DA groups (p < 0.05). In summary, the ZEN-contaminated diet had a damaging impact on growth performance, plasma immune function and hormone secretion of gilts. Although probiotic and biodegradable Bacillus subtilis have similar antimicrobial capacities, only biodegradable Bacillus subtilis could eliminate these negative effects through its biodegradable property to ZEN.

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