scholarly journals Effects of Dietary Supplementation with Epigallocatechin Gallate on Meat Quality and Muscle Antioxidant Capacity of Broilers Subjected to Acute Heat Stress

Animals ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3296
Author(s):  
Fei Zhao ◽  
Xiaocheng Wang ◽  
Yang Li ◽  
Xingyong Chen ◽  
Zhaoyu Geng ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Heat Stress ◽  
Antioxidant Capacity ◽  
Meat Quality ◽  
Free Group ◽  
Mrna Level ◽  
Drip Loss ◽  
Mrna Levels ◽  
Sod Activity ◽  
Acute Heat Stress

This study evaluated epigallocatechin gallate’s (EGCG’s, 400 mg/kg) effect on meat quality and muscle antioxidant status of broilers under acute heat stress (AHS). A total of 144 21-day-old male Huainan partridge chickens were randomly allocated to the EGCG-free group (12 replicates) and the EGCG group (6 replicates). On day 94, the EGCG-free group was divided into the control group (CON) and the AHS group, and then AHS group and EGCG group (identified as AHS + EGCG group) were treated with AHS (33 ± 1 °C for 12 h). AHS increased (p < 0.05) L*24h, drip loss, muscle lactic acid, malondialdehyde (MDA) contents, and kelch-like ECH-associated protein 1 (Keap1) mRNA level, and decreased (p < 0.05) eviscerated percentage, pH24h, a*, muscle total superoxide dismutase (T-SOD) activity, the ratio of T-SOD/MDA and glutathione peroxidase /MDA, glycogen content, and nuclear factor erythroid 2-related 2 (Nrf2), catalase (CAT), NAD(P)H/quinone dehydrogenase 1 (NQO1) mRNA levels. The AHS + EGCG group exhibited lower (p < 0.05) L*24h, drip loss, muscle lactic acid, MDA contents and Keap1 mRNA level, and greater (p < 0.05) eviscerated percentage, pH24h, a*, muscle T-SOD activity, the ratio of T-SOD/MDA, Nrf2, and NQO1 mRNA levels compared with the AHS group. In conclusion, EGCG protects against AHS-impaired meat quality by improving muscle antioxidant capacity, which seems to be associated with the activation of the Nrf2 signaling pathway.

scholarly journals The effect of calnexin deletion on the expression level of PDI in Saccharomyces cerevisiae under heat stress conditions

2008 ◽  
Vol 13 (1) ◽  
Author(s):  
Huili Zhang ◽  
Jianwei He ◽  
Yanyan Ji ◽  
Akio Kato ◽  
Youtao Song
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Growth Rate ◽  
Heat Stress ◽  
Protein Expression ◽  
Molecular Chaperone ◽  
Mrna Level ◽  
Stress Conditions ◽  
Mrna Levels ◽  
Wild Type ◽  
Wild Type Yeast

AbstractWe cultured calnexin-disrupted and wild-type Saccharomyces cerevisiae strains under conditions of heat stress. The growth rate of the calnexin-disrupted yeast was almost the same as that of the wild-type yeast under those conditions. However, the induced mRNA level of the molecular chaperone PDI in the ER was clearly higher in calnexin-disrupted S. cerevisiae relative to the wild type at 37°C, despite being almost the same in the two strains under normal conditions. The western blotting analysis for PDI protein expression in the ER yielded results that show a parallel in their mRNA levels in the two strains. We suggest that PDI may interact with calnexin under heat stress conditions, and that the induction of PDI in the ER can recover part of the function of calnexin in calnexin-disrupted yeast, and result in the same growth rate as in wild-type yeast.

The effects of temperature elevation and water deprivation on lamb physiology, welfare, and meat quality

2002 ◽  
Vol 53 (6) ◽  
pp. 707 ◽  
Author(s):  
Tim E. Lowe ◽  
Neville G. Gregory ◽  
Andrew D. Fisher ◽  
Steven R. Payne
Keyword(s):  
Heat Stress ◽  
Meat Quality ◽  
Respiration Rate ◽  
Water Deprivation ◽  
Plasma Catecholamines ◽  
Additional Stress ◽  
Physiological Indicators ◽  
Acute Heat Stress ◽  
Humidity Index ◽  
Highly Correlated

Romney-cross ewe lambs (n = 27) were subjected to controlled environmental conditions to induce acute heat stress. The goals of the study were to: (1) determine appropriate physiological indicators of acute heat stress, (2) determine threshold rectal temperatures (Tr) for catecholamine and cortisol release, (3) determine effects on meat quality, and (4) assess the effect of dehydration on the above measures. There were 3 treatments: control (ambient temperature and humidity), heat stress (33°C, and 85-100% humidity), and heat stress combined with water deprivation. The duration of the treatment period was 12 h. Respiration rate (Rf) and rectal temperature (Tr) were highly correlated with increasing temperature humidity index (THI) (r > 0.75, P < 0.001), whereas heart rate was less responsive to THI (r = 0.30, P < 0.05). The welfare of these lambs was at risk at Tr greater than 40.5°C, a point at which respiration rate was maximal and unable to prevent further increases in Tr. Plasma cortisol concentrations were increased in heat-stressed lambs after Tr reached approximately 40.7°C. Plasma catecholamines were only elevated in lambs when Tr was greater than 42°C. The majority of lambs subjected to heat stress had a Tr less than 42°C, and there were no significant effects on meat quality. Despite exhibiting increases in plasma protein concentrations, there were no indications that dehydrated lambs were under additional stress during heat challenge in comparison with hydrated lambs.

scholarly journals Growth Performance and Characterization of Meat Quality of Broiler Chickens Supplemented with Betaine and Antioxidants under Cyclic Heat Stress

Antioxidants ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 336 ◽  
Author(s):  
Majid Shakeri ◽  
Jeremy J. Cottrell ◽  
Stuart Wilkinson ◽  
Hieu H. Le ◽  
Hafiz A. R. Suleria ◽  
...  
Keyword(s):  
Heat Stress ◽  
Product Quality ◽  
Meat Quality ◽  
Broiler Chickens ◽  
Thiobarbituric Acid ◽  
Respiratory Alkalosis ◽  
Drip Loss ◽  
Final Body Weight ◽  
Broiler Performance ◽  
Commercial Diet

Heat stress (HS) causes oxidative stress, which compromises broiler performance and meat quality. The aim of this study was to determine whether dietary antioxidants could be used as an amelioration strategy. Seventy-two day-old-male Ross-308 chicks were exposed to either thermoneutral or cyclical heat stress conditions. Diets were either control commercial diet (CON), CON plus betaine (BET), or with a combination of betaine, selenized yeast, and vitamin E (BET + AOX). Heat stress increased the rectal temperature (p < 0.001), respiration rate (p < 0.001), decreased blood pCO2 (p = 0.002), and increased blood pH (p = 0.02), which indicated the HS broilers had respiratory alkalosis. Final body weight was decreased by HS (p < 0.001), whereas it was improved with BET (p = 0.05). Heat stress reduced cooking loss (p = 0.007) and no effect on drip loss, while BET decreased the drip loss (p = 0.01). Heat stress reduced the myofibril fragmentation index (p < 0.001) and increased thiobarbituric acid reactive substances (p < 0.001), while these were improved with the combination of BET + AOX (p = 0.003). In conclusion, BET overall improved growth rates and product quality in this small university study, whereas some additional benefits were provided by AOX on product quality in both TN and HS broilers.

scholarly journals Effects of dietary licorice extract on serum Biochemical index, tissues antioxidant capacity and immunity function of weaned piglets

2020 ◽  
Author(s):  
You Ting ◽  
Tang Jia Yong ◽  
Jia Gang ◽  
Liu Guang Mang ◽  
Tian Gang ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Density Lipoprotein ◽  
Control Group ◽  
Mrna Levels ◽  
Weaned Piglets ◽  
Sod Activity ◽  
Inflammatory Genes ◽  
Tnf Α ◽  
Licorice Extract ◽  
Serum Biochemical

Abstract Background: This study investigated the effects of dietary licorice extract (LE) on antioxidant capacity and immunity in weaned piglets. A total of 96 DLY (Duroc × Landrace × Yorkshire) weaned piglets were randomly assigned to four treatments. The control group were fed a corn–soybean meal-based diet (basal diet, BD), and three LE level groups were fed on BD supplied with 50、150 and 250 mg/kg LE. The trial lasted 5 weeks. At day 35, six piglets per treatment were killed and blood, liver, spleen, and thymus were collected.Results: The result showed that: 1)Dietary LE increased (P < 0.05) activity of the alkaline phosphatase (ALP) and reduced (P < 0.05) the activity of glutamic oxalacetic transaminase (AST), 50 mg / kg LE reduced (P < 0.05) total cholesterol (TC) and high density lipoprotein cholesterol (HDL-C) in serum. 2) The addition of 150 and 250 mg / kg LE increased (P < 0.05) glutathion peroxidase (GSH-Px) activity in liver and spleen, increased (P < 0.05) the total antioxidant capacity (T-AOC) in serum and spleen . 50 mg / kg LE increased (P < 0.05) the total superoxide dismutase (T-SOD) activity in serum. Three doses of LE reduced (P < 0.05) serum malondialdehyde content (MDA). 3) 150 mg / kg LE increased (P < 0.05) serum IgG level. 4) Dietary LE down-regulated (P < 0.05) the mRNA levels of 7 immune-related genes (IL-6, IL-8, IL-10, IL-1β, TNF-α, MCP-1, ICAM-1) in the thymus; 50 mg / kg LE and 150 mg / kg LE down-regulated (P < 0.05) the mRNA levels of TNF-α, while 250 mg / kg LE up-regulated the mRNA levels of 2 inflammatory genes (IL-1β, and ICAM-1) in the spleen; three levels of LE down-regulated (P < 0.05 the mRNA levels of 3 inflammatory genes (IL-6, TNF-α, ICAM-1) in the liver.Conclusions: In summary, LE supplementation regulates the activity of serum biochemical enzyme, improves the antioxidant capacity and immune function of in serum, liver, spleen and thymus, those improvement may contributes to the promotion of growth performance of weaned piglets. In general, 150 mg / kg LE exhibits better effect.

scholarly journals Antioxidant Supplement Inhibits Skeletal Muscle Constitutive Autophagy rather than Fasting-Induced Autophagy in Mice

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Zhengtang Qi ◽  
Qiang He ◽  
Liu Ji ◽  
Shuzhe Ding
Keyword(s):  
Skeletal Muscle ◽  
Antioxidant Capacity ◽  
Manganese Superoxide Dismutase ◽  
Mrna Level ◽  
Mrna Levels ◽  
Ros Production ◽  
Muscle Loss ◽  
Manganese Superoxide ◽  
Mitochondrial Ros ◽  
Antioxidant Supplement

In this study, we tested the hypothesis that NAC administration leads to reduced oxidative stress and thus to decreased expression of autophagy markers in young mice. Our results reveal that NAC administration results in reduced muscle mRNA levels of several autophagy markers, including Beclin-1, Atg7, LC3, Atg9, and LAMP2. However, NAC supplement fails to block the activation of skeletal muscle autophagy in response to fasting, because fasting significantly increases the mRNA level of several autophagy markers and LC3 lipidation. We further examined the effects of NAC administration on mitochondrial antioxidant capacity in fed and 24-hour fasted mice. Our results clearly show that NAC administration depresses the expression of manganese superoxide dismutase (MnSOD) and TP53-induced glycolysis and apoptosis regulator (TIGAR), both of which play a predominant antioxidant role in mitochondria by reducing ROS level. In addition, we found no beneficial effect of NAC supplement on muscle mass but it can protect from muscle loss in response to fasting. Collectively, our findings indicate that ROS is required for skeletal muscle constitutive autophagy, rather than starvation-induced autophagy, and that antioxidant NAC inhibits constitutive autophagy by the regulation of mitochondrial ROS production and antioxidant capacity.

scholarly journals Effect of acute heat stress and slaughter processing on poultry meat quality and postmortem carbohydrate metabolism

2017 ◽  
Vol 96 (3) ◽  
pp. 738-746 ◽  
Author(s):  
R.H. Wang ◽  
R.R. Liang ◽  
H. Lin ◽  
L.X. Zhu ◽  
Y.M. Zhang ◽  
...  
Keyword(s):  
Heat Stress ◽  
Carbohydrate Metabolism ◽  
Meat Quality ◽  
Poultry Meat ◽  
Acute Heat Stress

scholarly journals Effects of Resveratrol on Growth Performance, Intestinal Development, and Antioxidant Status of Broilers under Heat Stress

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1427
Author(s):  
Chi Wang ◽  
Fei Zhao ◽  
Zhen Li ◽  
Xu Jin ◽  
Xingyong Chen ◽  
...  
Keyword(s):  
Heat Stress ◽  
Antioxidant Capacity ◽  
Growth Performance ◽  
Related Factor ◽  
Mrna Levels ◽  
Intestinal Development ◽  
Villus Height ◽  
Final Body Weight ◽  
Treatment Groups ◽  
Nrf2 Signaling Pathway

The study investigated resveratrol’s effect on growth performance, intestinal development, and antioxidant capacity of broilers subjected to heat stress (HS). A total of 162 21-day-old male AA broilers were randomly divided into 3 treatment groups with 6 replicates of 9 birds each. The 3 treatment groups were as follows: the control (CON), in which broilers were housed at 22 ± 1 °C for 24 h day−1, and the HS and HS + resveratrol (400 mg/kg) groups, in which broilers were housed at 33 ± 1 °C for 10 h a day from 8:00 to 18:00 and 22 ± 1 °C for rest of the time. Results indicated that birds in the HS group exhibited lower (p < 0.05) final body weight (BW) and average daily gain (ADG) compared with birds in the CON group. HS birds also had lower (p < 0.05) relative jejunum weight, relative ileum and jejunum length, jejunal villus height, and villus height to crypt depth ratios than the CON group. The activities of glutathione peroxidase (GPX), glutathione S-transferase (GST), superoxide dismutase (SOD), and the mRNA levels of NF-E2-related factor 2 (Nrf2), SOD1, and GPX were also lower (p < 0.05) in the HS than CON group. The HS group had higher (p < 0.05) protein carbonyl (PC) contents and Kelch-like ECH-associated protein 1 (Keap1) mRNA levels. Compared with HS group, the HS + resveratrol group exhibited higher (p < 0.05) BW and ADG, relative jejunum weight, relative length of ileum, jejunal villus height, activities of GPX and GST, and mRNA levels of Nrf2 and SOD1, but they had lower (p < 0.05) PC content and Keap1 mRNA levels. In conclusion, resveratrol can improve the intestinal development and antioxidant function of broilers under HS, and therefore improve growth performance. The mechanism by which resveratrol enhances the intestinal antioxidant capacity is mediated by Nrf2 signaling pathway.

Sign in / Sign up

Export Citation Format

Share Document