scholarly journals Clinical Evaluation of Creatine Kinase and Aspartate Aminotransferase for Monitoring Muscle Effort in Working Dogs in Different Simulated Fieldworks

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1879
Author(s):  
Giuseppe Spinella ◽  
Simona Valentini ◽  
Vincenzo Musella ◽  
Enrico Bortolotti ◽  
Mirella Lopedote
Keyword(s):  
Creatine Kinase ◽  
Aspartate Aminotransferase ◽  
Physiological Parameters ◽  
Inclusion Criteria ◽  
Search Activity ◽  
Fitness Level ◽  
Before And After ◽  
Working Dogs ◽  
Clinical Profiles ◽  
Unknown Area

The clinical profiles of muscle biomarkers (Creatine Kinase–CK-and Aspartate Aminotransferase–AST) performed during training may help in determining the fitness level of dogs and their potentiality to perform specific activities. This study investigated the potential variations of physiological parameters and muscular biomarkers in trained search and rescue dogs during search activity in two different areas. The aim was to verify the absence of any muscular enzymes after 20 min of search activity. The variations of physiological parameters (pulse rate; respiratory rate; rectal body temperature) and skeletal muscular biomarkers (CK and AST) were evaluated before and after search activity. Twenty-three trained dogs met the inclusion criteria and were divided into two groups. One group experienced search activity in a well-known area, while the second one in a similar, but unknown, area. The results for physiological parameters and skeletal muscular biomarkers values showed no significant differences between the two groups (p > 0.05), confirming that an effective conditioning protects against enzymatic alteration during a 20 min duration of submaximal activity.

scholarly journals Changes in Salivary Levels of Creatine Kinase, Lactate Dehydrogenase, and Aspartate Aminotransferase after Playing Rugby Sevens: The Influence of Gender

2020 ◽  
Vol 17 (21) ◽  
pp. 8165
Author(s):  
Álvaro González Fernández ◽  
Jose Enrique de la Rubia Ortí ◽  
Lorena Franco-Martinez ◽  
Jose Joaquín Ceron ◽  
Gonzalo Mariscal ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Lactate Dehydrogenase ◽  
Muscle Damage ◽  
Aspartate Aminotransferase ◽  
Perceived Exertion ◽  
Total Sample ◽  
Physical Contact ◽  
Borg Scale ◽  
Men And Women ◽  
Before And After

Rugby sevens is characterised by continuous exertion and great physical contact per unit of time, leading to muscle damage. It is important to identify markers that can quantify muscle damage in order to improve recovery strategies. The objective of this study was to evaluate the release dynamics of muscle damage markers creatine kinase (CK), lactate dehydrogenase (LDH), and aspartate aminotransferase (AST) in saliva samples when playing rugby sevens, analysing the influence of gender, during the rugby sevens university championship of Spain. The total sample included 27 athletes, divided into two teams of 14 men and 13 women between 18 and 31 years of age. CK, LDH, and AST were quantified from salivary samples collected from each athlete before and after three rugby sevens matches. The modified Borg scale of perceived exertion was also used after each match. When the results were analysed globally, there were no differences in CK and LDH before and after any match, but AST did show differences after two days of completing all matches. In terms of gender, the three enzymes showed different responses in men and women. Regarding the Borg scale, there were only significant differences between men and women after completing all mataches, with a greater perceived exertion in women. Based on our results, it can be stated that that serial matches of rugby sevens can cause changes of different magnitude in AST, CK and LDH activities in saliva, with AST showing the most significant variations and these changes are more pronounced in men than in women.

scholarly journals Halitosis in Children Undergoing Full Mouth Rehabilitation under General Anesthesia

Children ◽  
2021 ◽  
Vol 8 (2) ◽  
pp. 149
Author(s):  
Noura A. AlMadhi ◽  
Ayman M. Sulimany ◽  
Hamad A. Alzoman ◽  
Omar A. Bawazir
Keyword(s):  
General Anesthesia ◽  
Sulfur Compounds ◽  
Parental Consent ◽  
Inclusion Criteria ◽  
Breath Sample ◽  
Volatile Sulfur Compounds ◽  
Before And After ◽  
Full Mouth Rehabilitation ◽  
Volatile Sulfur

Interventions and management modalities of pediatric halitosis have been suggested in the literature, however, the effect of full mouth rehabilitation (FMR) under general anesthesia (GA) on pediatric halitosis was not reported. Therefore, the present study was conducted to investigate parents’ perceptions of their child’s halitosis before and after FMR under GA; and to evaluate the effect of FMR on clinical halitosis. Fifty-seven children between 3–8 years old, scheduled for FMR under GA, were included after satisfying the inclusion criteria and upon parental consent. Parents’ perception of halitosis in their children was evaluated using a standardized questionnaire and a breath sample was collected to assess the level of volatile sulfur compounds (VSCs) using OralChromaTM before and after FMR under GA. Sixty percent (n = 34) of the parents perceived halitosis in their children before FMR and about 80% (n = 27) of them reported improvement in halitosis after FMR. Clinical halitosis was detected in 84.2% (n = 48) of the sample before treatment. A statistically significant reduction in halitosis was found in 56.3% (n = 27) of the children after treatment (p < 0.001). In conclusion, majority of parents perceived an absence or reduction of halitosis in their children following FMR and significant improvement of clinical halitosis.

scholarly journals “Yokoyama procedure” efficacy on non-highly myopic patients with acquired esotropia and hypotropia

2019 ◽  
Vol 30 (4) ◽  
pp. 676-679
Author(s):  
Massimiliano Serafino ◽  
Matteo Scaramuzzi ◽  
Edoardo Villani ◽  
Paolo Nucci
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Axial Length ◽  
Lateral Rectus ◽  
Inclusion Criteria ◽  
Resonance Imaging ◽  
Spherical Equivalent Refraction ◽  
Before And After ◽  
Weighted Magnetic Resonance Imaging ◽  
The Mean

Objective: To assess the efficacy of “Yokoyama Procedure,” on non-highly myopic patients with acquired esotropia and hypotropia. Methods: The study involved 10 eyes of 5 patients with eso-hypotropia. Inclusion criteria were acquired esotropic-hypotropic strabismus with lateral rectus inferior displacement and superior rectus nasal displacement confirmed by magnetic resonance imaging, refractive errors between ±6 D, and axial length < 27 mm. Range of full duction movements and maximum angles of abduction-sursumduction was measured in each eye before and after surgery. All patients underwent T1- and T2-weighted magnetic resonance imaging. The surgery was aimed at creating a junction between the muscle bellies of the superior and lateral rectus muscles. This junction was made approximately 14 mm behind the insertions using a non-absorbable mersilene 5/0 suture (Yokoyama procedure). Results: Mean patient age was 64.8 ± 4.8 years. The mean globe axial length was 25.4 ± 0.76 mm and a mean corresponding spherical equivalent refraction of −3.7 ± 1.7 D was observed. Eight eyes on 10 had mild limitation in abduction, while the remaining 2 had no limitation. Three out of 10 eyes showed a moderate limited sursumduction, 5 eyes were categorized as mild, and the remaining 2 had no limitation. No evident post-operative limitation was present in any eye, in both abduction and sursumduction (p < 0.01). Pre-operative esotropia and hypotropia were, respectively, 32 ± 11 prismatic diopters and 25 ± 5 prismatic diopters, and they were significantly reduced after surgery as 9 ± 1.7 prismatic diopters and 6 ±1 prismatic diopters (p = 0.043), respectively. Conclusion: Yokoyama procedure is an effective, fast, reversible procedure to face eso-hypotropic acquired strabismus, even in patients with a clear magnetic resonance imaging displacement of superior and lateral rectus muscles, and absence of globe dislocation and of elevated myopia.

Production and certification of secondary enzyme reference materials (ERMs). Part 1: Preparation of the sera and some of their properties.

1986 ◽  
Vol 32 (10) ◽  
pp. 1901-1905 ◽  
Author(s):  
J C Koedam ◽  
G M Steentjes ◽  
S Buitenhuis ◽  
E Schmidt ◽  
R Klauke
Keyword(s):  
Creatine Kinase ◽  
Lactate Dehydrogenase ◽  
Reference Material ◽  
Human Serum ◽  
Dehydrogenase Activity ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Glutamate Dehydrogenase Activity ◽  
The Stability ◽  
Clinical Enzymology

Abstract We produced three batches of a human-serum-based enzyme reference material (ERM) enriched with human aspartate aminotransferase (EC 2.6.1.1), alanine aminotransferase (EC 2.6.1.2), creatine kinase (EC 2.7.3.2), and lactate dehydrogenase (EC 1.1.1.27). The added enzymes were not exhaustively purified; thus the final ERMs contained some enzymes as contaminants, of which only glutamate dehydrogenase activity might interfere. The stability during storage and after reconstitution was good. The commutability of the four enzymes in the three ERM batches was also good, except when German or Scandinavian methods for aminotransferases were involved. The temperature-conversion factors for the ERMs were equivalent to those for patients' sera. Reactivation after reconstitution was complete within 5 min and was independent of the temperature of the reconstitution fluid. We believe that these secondary ERMs will aid in the transfer of accuracy between well-defined reference methods and daily working methods so that clinical enzymology results will become more comparable from laboratory to laboratory.

scholarly journals Local myotoxicity of ketamine hydrochloride in the marmoset

1987 ◽  
Vol 21 (1) ◽  
pp. 60-67 ◽  
Author(s):  
C. W. Davy ◽  
P. N. Trennery ◽  
J. G. Edmunds ◽  
J. F. B. Altman ◽  
D. A. Eichler
Keyword(s):  
Creatine Kinase ◽  
Lactate Dehydrogenase ◽  
Physical Properties ◽  
Aspartate Aminotransferase ◽  
Solution Ph ◽  
Histopathological Findings ◽  
Plasma Enzyme ◽  
Routine Blood ◽  
Ketamine Hydrochloride ◽  
Injection Procedure

An investigation of raised plasma aspartate aminotransferase (AST) in marmosets after intramuscular ketamine injection suggested a local myotoxicity. This was confirmed by a range of histopathological findings from myofibrillar striation loss to necrosis. In addition to the elevations in AST levels, creatine kinase and the lactate dehydrogenase-5 isoenzyme levels were elevated. It was further demonstrated that, although the physical properties of the injectable solution (pH, osmolality) and to a lesser extent the injection procedure itself caused slight changes in plasma enzyme levels, the ketamine was predominantly responsible for the lesion. No hepatic interactions were seen. This effect should be taken into consideration when this anaesthetic is used in the marmoset if the primary objectives of the experiment entail routine blood analyses.

Effect of Earmuffs on Physiological Parameters of Preterm Neonates Nursed in Incubators: A Before-and-After Study

2021 ◽  
Vol 58 (10) ◽  
pp. 943-946
Author(s):  
Amanpreet Kaur ◽  
Sukhwinder Kaur ◽  
Sanjay Munjal ◽  
Sandhya Ghai ◽  
Sourabh Dutta
Keyword(s):  
Physiological Parameters ◽  
Preterm Neonates ◽  
Before And After ◽  
Before And After Study

scholarly journals Effects of small-sided games and conventional aerobic interval training on various physiological characteristics and defensive and offensive skills used in soccer

Kinesiology ◽  
2018 ◽  
Vol 50 (1) ◽  
pp. 104-111 ◽  
Author(s):  
İlker Özcan ◽  
Çağatay Şahan
Keyword(s):  
Anaerobic Threshold ◽  
Interval Training ◽  
Endurance Performance ◽  
Physiological Parameters ◽  
Technical Ability ◽  
Aerobic Interval Training ◽  
Before And After ◽  
Analysis System ◽  
The Individual ◽  
Passing Ability

The aim of this study was to investigate the effects of small-sided game training (SSGT) versus conventional aerobic interval training (CAIT) on soccer-specific endurance performance, lactate threshold levels (mmol·L-1), short-passing ability and defensive and offensive skills of a soccer match. Before and after a 6-week training intervention period, eighteen amateur soccer players (age 21.8±4.8 years) were tested. The tests included the anaerobic threshold (AnT) test, the Yo-Yo Intermittent Recovery Test Level 1 (Yo-Yo IR1), and the Loughborough Soccer Passing Test (LSPT). A manual notational match analysis system was utilized to evaluate the defensive and offensive skills of players during the matches. Both the SSGT and CAIT were performed two days a week and consisted of five sets of 6-minute periods of work at the individualized exercise intensity corresponding to the individual anaerobic threshold, with 3-minute of recovery periods&nbsp;between sets. Mann-Whitney U and Wilcoxon signed rank tests were used to examine the between- and within-group differences, respectively. Statistical analyses revealed that the SSGT group players exhibited significantly better in terms of the LSPT scores (p&lt;.01) and the number of defensive and offensive skills (p&lt;.05). However, no other significant differences in the other variables were observed (p&gt;.05). The results of the study suggested that SSGT improved short-passing ability, various soccer skills and physiological parameters, while CAIT only improved physiological parameters. SSGT improves soccer-specific endurance and technical ability of players at the same time, meaning it is a time efficient way of training.

scholarly journals Ischemia-Reperfusion Intervention: From Enhancements in Exercise Performance to Accelerates Performance Recovery - A Systematic Review and Meta-Analysis

2020 ◽  
Author(s):  
Rhaí André Arriel ◽  
Jéssica Ferreira Rodrigues ◽  
Hiago Leandro Rodrigues de Souza ◽  
Anderson Meireles ◽  
Luís Filipi Moutinho Leitão ◽  
...  
Keyword(s):  
Systematic Review ◽  
Creatine Kinase ◽  
Exercise Performance ◽  
Muscle Soreness ◽  
Ischemia Reperfusion ◽  
Meta Analysis ◽  
Ischemic Conditioning ◽  
Fitness Level ◽  
Positive Effects ◽  
Performance Recovery

It has been demonstrated that brief cycles of ischemia followed by reperfusion (IR) applied before exercise can improve performance and, IR intervention, applied immediately after exercise (post-exercise ischemic conditioning &ndash; PEIC) exerts a potential ergogenic effect to accelerate recovery. Thus, the purpose of this systematic review with meta-analysis was to identify the effects of PEIC on exercise performance, recovery and the responses of associated physiological parameters, such as creatine kinase, perceived recovery and muscle soreness, over 24 h after its application. From 3281 studies, six involving 106 subjects fulfilled the inclusion criteria. Compared to sham (cuff administration with low pressure) and control interventions (no cuff administration), PEIC led to faster performance recovery (p=0.004; ES=-0.49) and lower increase in creatine kinase (p&amp;lt;0.001; ES=-0.71) and muscle soreness (p&amp;lt;0.001; ES=-0.89) over 24 h. The effectiveness of this intervention is more pronounced in subjects with low/moderate fitness level and at least a total time of 10 min of ischemia (e.g. 2 cycles of 5 min) is necessary to promote positive effects.

scholarly journals TELEMEDICINE AS AN EFFORT TO IMPROVE PUBLIC KNOWLEDGE TO FIGHT COVID-19 PANDEMIC

2021 ◽  
Vol 2 (1) ◽  
pp. 6
Author(s):  
Sacharissa Zerlina Tsarwah Thirafi ◽  
Triadi Putra Paladan ◽  
Bonfilio Neltio Ariobimo ◽  
Barizatul Husniyah ◽  
Monica Tiara Arum Kinanthi ◽  
...  
Keyword(s):  
Health Promotion ◽  
Data Collection ◽  
Sampling Method ◽  
Public Knowledge ◽  
Inclusion Criteria ◽  
Collection Method ◽  
Before And After ◽  
Chain Transmission ◽  
A Current

The role of the community is an essential part to cut down the COVID-19 chain transmission, a current world pandemic. Knowledge determines a person's healthy behavior that can prevent transmission of COVID-19. This research’s goal is to analyze the effect of health promotion via telemedicine on increasing knowledge about COVID-19 prevention in the productive-age population in Indonesia. This study used one group pretest-posttest design and accidental sampling method involving 146 respondents based on the inclusion criteria. The data collection method was carried out by using a questionnaire containing simple and general questions related to COVID-19 that tested subjects before and after telemedicine health promotion. Based on the assessment of the questionnaire through pretest and posttest, there was an increase in the average knowledge after being given health education with telemedicine with p<0.001 (CI 95%). This study shows that telemedicine could significantly increase average knowledge about COVID-19 prevention in the productive-age population in Indonesia.

Sign in / Sign up

Export Citation Format

Share Document