scholarly journals Effect of Replacing in-Feed Antibiotic Growth Promoters with a Combination of Egg Immunoglobulins and Phytomolecules on the Performance, Serum Immunity, and Intestinal Health of Weaned Pigs Challenged with Escherichia coli K88

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1292
Author(s):  
Yunsheng Han ◽  
Tengfei Zhan ◽  
Chaohua Tang ◽  
Qingyu Zhao ◽  
Dieudonné M. Dansou ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Basal Diet ◽  
Intestinal Health ◽  
Post Challenge ◽  
Growth Promoters ◽  
Villus Height ◽  
Weaned Pigs ◽  
E Coli ◽  
Antibiotic Growth Promoters ◽  
Diet Supplementation

The study was conducted to investigate the effects of replacing antibiotic growth promoters (AGPs) with an egg immunoglobulin (IgY) combined with phytomolecules (PM) on the growth rate, serum immunity, and intestinal health of weaned pigs challenged with Escherichia coli K88 (E. coli K88). A total of 192 piglets were weaned at 28 days old with an average weight of 7.29 (± 0.04) kg. They were randomly divided into four treatments containing eight replicates with six piglets per replicate. The treatment groups were NC and PC fed a basal diet, AGP fed a basal diet supplemented with 75 mg/kg chlortetracycline, 50 mg/kg oxytetracycline calcium, and 40 mg/kg zinc bacitracin, IPM fed a basal diet supplemented with IgY at dose of 2.5 g/kg and 1.0 g/kg and PM at dose of 300 mg/kg and 150 mg/kg during days 1 to 17 and 18 to 42, respectively. On days 7 to 9 of the experiment, piglets in the PC, AGP, and IPM groups were orally challenged with 20 mL E. coli K88 (109 CFU/mL), while piglets in the NC group were challenged with 20 mL medium without E. coli K88. The E. coli K88 challenge model was successful as the incidence of post-weaning diarrhea (PWD) of piglets challenged with E. coli K88 was significantly higher than that of those unchallenged piglets during the challenge time (days 7 to 9) and days 1 to 7 of post-challenge (p < 0.05). A diet with combinations of IgY and PM and AGPs significantly decreased the incidence of PWD during the challenge time and days 1 to 7 of post-challenge (p < 0.05) compared to the PC group and significantly improved the ratio of feed to weight gain (F:G) during days 1 to 17 of the experiment compared to the NC and PC groups (p < 0.05). In comparison with the PC group, piglets in the IPM group had significantly higher serum levels of IgA, IgG, and IgM (p < 0.05), but lower serum IL-1β on day 17 of experiement (p < 0.05). Besides, diet supplementation with AGP significantly decreased serum IL-1β, IL-6, and TNF-α on days 17 and 42 (p < 0.05) with comparison to the PC group. Piglets in the IPM group showed a significantly lower level of fecal coliforms (p < 0.05), but a higher villus height of jejunum and ileum and higher ratio of villus height to crypt depth of duodenum and jejunum (p < 0.05) than those piglets in the PC group. In summary, diet supplementation with a mixture of IgY and PM decreased the incidence of PWD and coliforms, increased feed conversion ratio, and improved intestinal histology and immune function.

scholarly journals Growth performance and gut health of Escherichia coli-challenged weaned pigs fed diets supplemented with a Bacillus subtilis direct-fed microbial

2020 ◽  
Vol 4 (3) ◽  
Author(s):  
Sangwoo Park ◽  
Jung Wook Lee ◽  
Kevin Jerez Bogota ◽  
David Francis ◽  
Jolie Caroline González-Vega ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Growth Performance ◽  
Feed Efficiency ◽  
Basal Diet ◽  
Feed Additives ◽  
Growth Promoter ◽  
Gut Health ◽  
Weaned Pigs ◽  
E Coli

Abstract This study was conducted to investigate the effects of a direct-fed microbial (DFM) product (Bacillus subtilis strain DSM 32540) in weaned pigs challenged with K88 strain of Escherichia coli on growth performance and indicators of gut health. A total of 21 weaned pigs [initial body weight (BW) = 8.19 kg] were housed individually in pens and fed three diets (seven replicates per diet) for 21 d in a completely randomized design. The three diets were a corn-soybean meal-based basal diet without feed additives, a basal diet with 0.25% antibiotics (neo-Oxy 10-10; neomycin + oxytetracycline), or a basal diet with 0.05% DFM. All pigs were orally challenged with a subclinical dose (6.7 × 108 CFU/mL) of K88 strain of E. coli on day 3 of the study (3 d after weaning). Feed intake and BW data were collected on days 0, 3, 7, 14, and 21. Fecal scores were recorded daily. On day 21, pigs were sacrificed to determine various indicators of gut health. Supplementation of the basal diet with antibiotics or DFM did not affect the overall (days 0–21) growth performance of pigs. However, antibiotics or DFM supplementation increased (P = 0.010) gain:feed (G:F) of pigs during the post-E. coli challenge period (days 3–21) by 23% and 24%, respectively. The G:F for the DFM-supplemented diet did not differ from that for the antibiotics-supplemented diet. The frequency of diarrhea for pigs fed a diet with antibiotics or DFM tended to be lower (P = 0.071) than that of pigs fed the basal diet. The jejunal villous height (VH) and the VH to crypt depth ratio (VH:CD) were increased (P &lt; 0.001) by 33% and 35%, respectively, due to the inclusion of antibiotics in the basal diet and by 43% and 41%, respectively due to the inclusion of DFM in the basal diet. The VH and VH:CD for the DFM-supplemented diet were greater (P &lt; 0.05) than those for the antibiotics-supplemented diet. Ileal VH was increased (P &lt; 0.05) by 46% due to the inclusion of DFM in the basal diet. The empty weight of small intestine, cecum, or colon relative to live BW was unaffected by dietary antibiotics or DFM supplementation. In conclusion, the addition of DFM to the basal diet improved the feed efficiency of E. coli-challenged weaned pigs to a value similar to that of the antibiotics-supplemented diet and increased jejunal VH and VH:CD ratio to values greater than those for the antibiotics-supplemented diet. Thus, under E. coli challenge, the test DFM product may replace the use of antibiotics as a growth promoter in diets for weaned pigs to improve feed efficiency and gut integrity.

Manno-oligosaccharide attenuates inflammation and intestinal epithelium injury in weaned pigs upon Enterotoxigenic Escherichia Coli K88 challenge

2020 ◽  
pp. 1-37
Author(s):  
En Yu ◽  
Daiwen Chen ◽  
Bing Yu ◽  
Zhiqing Huang ◽  
Xiangbing Mao ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Intestinal Epithelium ◽  
Elevated Serum ◽  
Basal Diet ◽  
Enterotoxigenic Escherichia Coli ◽  
Serum Concentrations ◽  
Villus Height ◽  
Weaned Pigs ◽  
Expression Levels ◽  
Glucose Transporter 2

Abstract To explore the effect of mannan-oligosaccharide (MOS) on intestinal health in weaned pigs upon Enterotoxigenic Escherichia coli K88 (ETEC) challenge, thirty-two male weaned pigs were randomly assigned into four groups. Pigs fed with a basal diet or basal diet containing MOS (0.6 g/kg) were orally infused with ETEC or culture medium. Results showed that MOS significantly elevated the digestibility of crude protein (CP) and gross energy (GE) in both ETEC-challenged and non-challenged pigs (P < 0.05). MOS also elevated serum concentrations of immunoglobulin (Ig) A and IgM (P < 0.05), but decreased serum concentrations of tumor necrosis factor (TNF)-α, IL-1β, and IL-6 (P < 0.05) in ETEC-challenged pigs. Interestingly, MOS increased villus height and the ratio of villus height to crypt depth (V/C) in duodenum and ileum (P < 0.05). MOS also increased duodenal sucrase and ileal lactase activity in ETEC-challenged pigs (P < 0.05). MOS decreased the abundance of Escherichia coli, but increased the abundance of Lactobacillus, Bifidobacterium, and Bacillus in cecum (P < 0.05). Importantly, MOS not only elevated the expression levels of zonula occludins-1 (ZO-1), claudin-1, and glucose transporter-2 (GLUT-2) in duodenum (P < 0.05), but also elevated the expression levels of ZO-1, GLUT-2, and L-type amino acid transporter-1 (LAT-1) in ileum (P < 0.05) upon ETEC challenge. These results suggested that MOS can alleviate inflammation and intestinal injury in weaned pigs upon ETEC challenge, which was associated with suppressed secretion of inflammatory cytokines and elevated serum immunoglobulins, as well as improved intestinal epithelium functions and microbiota.

scholarly journals Trace amounts of antibiotic exacerbated diarrhea and systemic inflammation of weaned pigs infected with a pathogenic Escherichia coli

2021 ◽  
Vol 99 (3) ◽  
Author(s):  
Kwangwook Kim ◽  
Yijie He ◽  
Cynthia Jinno ◽  
Lauren Kovanda ◽  
Xunde Li ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Tumor Necrosis Factor ◽  
Blood Cell ◽  
Tumor Necrosis ◽  
Basal Diet ◽  
Weaned Pigs ◽  
Ileal Mucosa ◽  
E Coli ◽  
Mesenteric Lymph ◽  
Necrosis Factor

Abstract The experiment was conducted to investigate the effects of trace amounts of antibiotic on growth performance, diarrhea, systemic immunity, and intestinal health of weaned pigs experimentally infected with an enterotoxigenic Escherichia coli. Weaned pigs (n = 34, 6.88 ± 1.03 kg body weight [BW]) were individually housed in disease containment rooms and randomly allotted to one of the three dietary treatments: nursery basal diet (CON) and two additional diets supplemented with 0.5 or 50 mg/kg carbadox to the nursery basal diet (TRA or REC), respectively. The experiment lasted 18 d with 7 d before and 11 d after the first E. coli inoculation. The E. coli F18 inoculum was orally provided to all pigs with a dose of 1010 colony-forming unit (CFU)/3 mL for three consecutive days. Fecal and blood samples were collected on day 0 before inoculation and days 2, 5, 8, and 11 postinoculation (PI) to test the percentage of β-hemolytic coliforms in total coliforms and complete blood cell count, respectively. Sixteen pigs were euthanized on day 5 PI, whereas the remaining pigs were euthanized at the end of the experiment to collect the jejunal and ileal mucosa and mesenteric lymph node for gene expression and bacterial translocation, respectively. Pigs in REC had greater (P &lt; 0.05) final BW and lower (P &lt; 0.05) overall frequency of diarrhea compared with pigs in the CON and TRA groups. Pigs in TRA had the lowest (P &lt; 0.05) average daily gain and feed efficiency from day 0 to 5 PI, highest (P &lt; 0.05) percentage of β-hemolytic coliforms in fecal samples on days 2 and 5 PI, and greatest (P &lt; 0.05) bacterial colonies in mesenteric lymph nodes on day 11 PI compared with pigs in the CON and REC groups. Pigs in TRA had the greatest (P &lt; 0.05) neutrophils on day 5 PI and higher (P &lt; 0.05) white blood cell counts and lymphocytes than other groups on day 11 PI. Pigs in TRA had the greatest (P &lt; 0.05) serum C-reactive protein on days 2 and 5 PI and serum tumor necrosis factor-α on day 5 PI, compared with pigs in the CON and REC groups. Pigs fed REC had increased (P &lt; 0.05) mRNA expression of zona occludens-1 (ZO-1) and occludin (OCDN) and reduced (P &lt; 0.05) interleukin-1 beta (IL1B), interleukin-6 (IL6), and tumor necrosis factor-alpha (TNFA) in ileal mucosa on day 5 PI, compared with the CON, whereas TRA upregulated (P &lt; 0.05) mRNA expression of IL1B, IL6, and cyclooxygenase-2 (COX2) in the ileal mucosa on day 11 PI, compared with the REC. In conclusion, trace amounts of antibiotic may exacerbate the detrimental effects of E. coli infection on pig performance by increasing diarrhea and systemic inflammation of weanling pigs.

scholarly journals Efficacy of organic acids as an alternative to antibiotic growth promoters in weaned pigs

2018 ◽  
Vol 17 (06) ◽  
pp. 8-14
Author(s):  
Tung M. Che
Keyword(s):  
Organic Acids ◽  
Antibiotic Treatment ◽  
Average Daily Gain ◽  
Basal Diet ◽  
Positive Control ◽  
Negative Control ◽  
Growth Promoters ◽  
Weaned Pigs ◽  
E Coli ◽  
Diarrhea Incidence

The objective of the experiment was to compare effects of dietary supplementation of organic acids (ProHacid Advance, PRO) and antibiotics on growth performance, diarrhea incidence, frequency of antibiotic treatment, and E. coli shedding in weaned pigs. A total of 224 crossbred weaned pigs [(Yorkshire x Landrace) x Duroc; 29 days old)] were allotted to 1 of 4 treatments with 7 replicate pens of 8 pigs each according to sex, litter origin and weight in an RCBD. The 4 dietary treatments included (1) basal diet + 50 mg/kg neomycin and 10 mg/kg enramycin (positive control, PC), (2) basal diet without antibiotics (negative control, NC), (3) As 2 + 0.25% PRO, and (4) As 2 + 0.5% PRO. Over a 4-week study, the results showed that there were no differences in the average daily gain and average daily feed intake of pigs among the 4 treatments (P = 0.328). However, pigs fed the PC diet (1.642) and 0.25% PRO-supplemented diet (1.641) had a lower feed to gain ratio (P < 0.05) than those fed the NC diet (1.808). The diarrhea incidence of pigs fed the 0.25% PRO-supplemented diet (7.36%) was lower (P < 0.01) than that of pigs fed the PC diet (11.61%), NC diet (16.70%), and 0.5% PRO-supplemented diet (10.08%). The frequency of antibiotic treatment of pigs consuming the 0.25% PRO-supplemented diet (4.67%) was lower (P < 0.01) than that of pigs consuming the PC diet (7.33%), NC diet (9.53%) and 0.5% PRO-supplemented diet (7.65%). No differences were found in the number of fecal E. coli among the 4 treatments. In brief, 0.25% PRO added to a nursery pig diet would be considered a potential alternative to the use of antibiotics in feed, but further research on this aspect is needed

scholarly journals PSVII-42 Growth performance, gut health, and immune responses of Escherichia coli-challenged weaned pigs fed probiotic-supplemented diets

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 359-360
Author(s):  
Sangwoo Park ◽  
Jinsu Hong ◽  
David Francis ◽  
Caroline J González -Vega ◽  
John K Htoo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Growth Performance ◽  
Immunoglobulin A ◽  
Dietary Treatment ◽  
Basal Diet ◽  
Gut Health ◽  
Weaned Pigs ◽  
E Coli ◽  
Randomized Design ◽  
Villous Height

Abstract To evaluate the effects of probiotics on performance and gut health of Escherichia coli-challenged weaned pigs, a total of 28 weaned pigs (initial BW = 8.25 kg) were housed individually in pens and fed 4 experimental diets for 21 d in a completely randomized design. The 4 diets were corn-soybean meal-based basal diet without or with 0.25% antibiotics (neomycin + oxytetracycline), 0.05% Bacillus subtilis DSM 32540, or 0.05% Bacillus pumilus DSM 32539. All pigs were orally challenged with K88 strain of E. coli on d 3. Feed intake and BW data, and blood samples were collected on d 3, 7, 14, and 21. Fecal scores were recorded daily. On d 21, pigs were sacrificed to determine gastrointestinal histomorphology, pH and organ weights. Supplementation of the basal diet with antibiotics or B. pumilus did not affect the overall (d 0 to 21) ADG and G:F. The B. subtilis supplementation did not affect the overall ADG, but tended to increase (P &lt; 0.1) the overall G:F by 15%. Fecal score was unaffected by dietary treatment. Supplementation of the basal diet with either probiotic, but not antibiotics increased (P &lt; 0.05) jejunal villous height and reduced (P &lt; 0.05) cecal digesta pH. Supplementation with B. pumilus tended to increase (P &lt; 0.1) colon weight relative to BW; however, antibiotics or B. subtilis supplementation did not affect colon weight. The addition of B. subtilis to basal diet tended to increase (P &lt; 0.1) serum immunoglobulin A concentration on d 21 of the study; however, such effect was not observed with the addition of antibiotics or B. pumilus. In conclusion, dietary B. subtilis improved G:F and jejunal villous height and reduced cecal digesta pH, indicating that dietary supplementation with B. subtilis at 0.05% could improve growth performance and gut health of weaned pigs.

scholarly journals Comparative Antibiotic and Probiotic Effects on Antimicrobial Sensitivity of Escherichia coli Isolates and Performance of Broiler Chickens

2021 ◽  
Vol 22 (3) ◽  
pp. 126-136
Author(s):  
Cosmas Chikezie Ogbu ◽  
Nwabueze Ibeneme
Keyword(s):  
Escherichia Coli ◽  
Body Weight ◽  
Feed Intake ◽  
Broiler Chickens ◽  
Basal Diet ◽  
Broiler Chicks ◽  
Microbial Count ◽  
Growth Promoters ◽  
Significant Treatment ◽  
E Coli

The study evaluated the growth performance, haematology, serum biochemistry, intestinal microbial count, and antimicrobial resistance profile of Escherichia coli (E. coli) from broiler chicks fed diets supplemented with antibiotics (neomycin, and oxytetracycline), and probiotic (Saccharomyces cerevisiae). One hundred and twenty Abor acre broiler chicks randomly alloted to four treatment groups (30 birds/group; 10 birds/replicate) were used in the 49 days study. Group one (G 1, control) were fed basal diet while G 2, 3, and 4 received basal diet containing S. cerevisiae (0.80g/kg; 108cfu/g), neomycin (0.50g/kg) and oxytetracycline (0.30g/kg), respectively. Results showed significant treatment effects on body weight, feed intake, linear body values, some haematological indices, intestinal, caecal and combined caecal and intestinal bacteria counts, diameter of E. coli inhibition zone, and mortality. Body weight and feed intake were significantly  higher in the supplemented groups. Intestinal bacterial count was highest in neomycin and control groups (5.29 ± 0.01 and 5.22 ± 0.02 Log10 cfu/ml, respectively) while S. cerevisiae and neomycin groups yielded the highest caecal, and combined caecal and intestinal bacterial counts. Eimeria Oocyst count did not differ significantly between groups. Escherichia coli from antibiotic fed groups had reduced sensitivity or were resistant to the antibiotics. It was concluded that subtherapeutic use of antibiotics as growth promoters in broiler chickens caused the development of antibiotic resistance, and therefore, should be avoided.

scholarly journals Effects of Achyranthes bidentata Polysaccharides on Intestinal Morphology, Immune Response, and Gut Microbiome in Yellow Broiler Chickens Challenged with Escherichia coli K88

Polymers ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 1233 ◽  
Author(s):  
Zhuying Liu ◽  
Xiaolong Wang ◽  
Shuqi Ou ◽  
Muhammed Arowolo ◽  
De-Xing Hou ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Immune Response ◽  
Community Structure ◽  
Basal Diet ◽  
Serum Glucose ◽  
Intestinal Morphology ◽  
Villus Height ◽  
E Coli ◽  
Tnf Α ◽  
Mucosal Morphology

The present study was conducted to investigate the effects of dietary Achyranthes bidentata polysaccharide (ABPS) supplementation on performance, immune response, intestinal mucosal morphology, and gut microbiome in yellow-feathered broilers challenged with Escherichia coli K88. A 2 × 2 factorial design was used for the trial. Two hundred and forty one-day-old female broilers were randomly assigned to four treatments: (1) negative-control broilers were fed by a basal diet and saline (NG); (2) positive-control broilers were fed by a basal diet and orally challenged with 108 CFU E. coli K88 (CNG); (3) ABP group broilers were fed by a basal diet containing ABPS (500 mg/kg of feed) and saline; (4) CABP group broilers were fed by a basal diet containing ABPS (500 mg/kg of feed) and orally challenged with 108 CFU E. coli K88. Growth performance, serum biochemical indexes, immune responses, intestinal mucosal morphology, and cecal microbial community structure were evaluated. The ABP group had greatest body weight (BW), average daily body weight gain (ADG), and intestinal villus height compared to other treatments (p < 0.05). The CABP group had a higher villus height/crypt depth ratio (V/C) compared with other treatments (p < 0.05). The expression levels of NF-κB were lower in the ABP group. The CNG group had higher Escherichia coli and Enterococcus contents in cecal samples compared to other treatments (p < 0.05). Serum glucose, uric acid, TNF-α, and Secretory Immunoglobulin A (S-IgA) levels were higher in broilers challenged with E. coli (p < 0.001) than that with saline. Broilers challenged with E. coli had reduced taxa richness in the cecal samples. Sequencing of 16S rRNA genes in cecal samples revealed that a lower proportion of Firmicutes and a higher proportion of Proteobacteria were detected in the broilers challenged with E. coli. Compared with the controls, dietary ABPS supplementation increased serum total protein, albumin, and S-IgA levels, but decreased serum glucose, uric acid, and TNF-α levels in broilers (p < 0.05). Diet supplemented with ABPS increased the Firmicutes/Bacteroidetes ratio and the abundance of Ruminococcaceae and Lachnospiraceae, and altered cecal microbiota community structure. These results suggest that ABPS can promote growth performance and improve intestinal morphology and microbiota community structure in broilers challenged with E. coli K88.

scholarly journals Impact of Feed Supplementation with Antimicrobial Agents on Growth Performance of Broiler Chickens, Clostridium perfringens and Enterococcus Counts, and Antibiotic Resistance Phenotypes and Distribution of Antimicrobial Resistance Determinants in Escherichia coli Isolates

2007 ◽  
Vol 73 (20) ◽  
pp. 6566-6576 ◽  
Author(s):  
Moussa S. Diarra ◽  
Fred G. Silversides ◽  
Fatoumata Diarrassouba ◽  
Jane Pritchard ◽  
Luke Masson ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Broiler Chickens ◽  
Antimicrobial Agents ◽  
Growth Promoters ◽  
Feed Supplementation ◽  
E Coli ◽  
Resistance Determinants ◽  
Class 1

ABSTRACT The effects of feed supplementation with the approved antimicrobial agents bambermycin, penicillin, salinomycin, and bacitracin or a combination of salinomycin plus bacitracin were evaluated for the incidence and distribution of antibiotic resistance in 197 commensal Escherichia coli isolates from broiler chickens over 35 days. All isolates showed some degree of multiple antibiotic resistance. Resistance to tetracycline (68.5%), amoxicillin (61.4%), ceftiofur (51.3%), spectinomycin (47.2%), and sulfonamides (42%) was most frequent. The levels of resistance to streptomycin, chloramphenicol, and gentamicin were 33.5, 35.5, and 25.3%, respectively. The overall resistance levels decreased from day 7 to day 35 (P < 0.001). Comparing treatments, the levels of resistance to ceftiofur, spectinomycin, and gentamicin (except for resistance to bacitracin treatment) were significantly higher in isolates from chickens receiving feed supplemented with salinomycin than from the other feeds (P < 0.001). Using a DNA microarray analysis capable of detecting commonly found antimicrobial resistance genes, we characterized 104 tetracycline-resistant E. coli isolates from 7- to 28-day-old chickens fed different growth promoters. Results showed a decrease in the incidence of isolates harboring tet(B), bla TEM, sulI, and aadA and class 1 integron from days 7 to 35 (P < 0.01). Of the 84 tetracycline-ceftiofur-resistant E. coli isolates, 76 (90.5%) were positive for bla CMY-2. The proportions of isolates positive for sulI, aadA, and integron class 1 were significantly higher in salinomycin-treated chickens than in the control or other treatment groups (P < 0.05). These data demonstrate that multiantibiotic-resistant E. coli isolates can be found in broiler chickens regardless of the antimicrobial growth promoters used. However, the phenotype and the distribution of resistance determinants in E. coli can be modulated by feed supplementation with some of the antimicrobial agents used in broiler chicken production.

187 Effects of Oligosaccharide-based Polymer on Growth Performance, Diarrhea, and Fecal β-hemolytic Coliforms in Weanling Pigs Experimentally Infected with a Pathogenic E. coli

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 90-90
Author(s):  
Kwangwook Kim ◽  
Yijie He ◽  
Cynthia Jinno ◽  
Seijoo Yang ◽  
Xunde Li ◽  
...  
Keyword(s):  
Growth Performance ◽  
Feed Efficiency ◽  
Basal Diet ◽  
Watery Diarrhea ◽  
Experimental Unit ◽  
Post Inoculation ◽  
Weaned Pigs ◽  
E Coli ◽  
Diet Control ◽  
Dietary Treatments

Abstract The objective of this experiment was to investigate dietary supplementation of oligosaccharide-based polymer on growth performance, diarrhea, and fecal β-hemolytic coliforms of weaned pigs experimentally infected with a pathogenic F18 Escherichia coli (E. coli). Forty-eight pigs (7.23 ± 1.11 kg BW) were individually housed in disease containment rooms and randomly allotted to one of four treatments with 12 replicate pigs per treatment. The four dietary treatments were a nursery basal diet (control), and 3 additional diets supplemented with 50 mg/kg Mecadox (AGP), 10 or 20 mg/kg of oligosaccharide-based polymer. The experiment lasted 18 d [7 d before and 11 d after the first inoculation (d 0)]. The doses of F18 E. coli inoculum were 1010 cfu/3 mL oral dose daily for 3 days. Growth performance was measured on d -7 to 0 before inoculation, and d 0 to 5 and 5 to 11 post-inoculation (PI). Diarrhea score (DS; 1, normal, to 5, watery diarrhea) was daily recorded for each pig. Fecal samples were collected on d 2, 5, 8, and 11 PI to test the percentage of β-hemolytic coliforms in total coliforms. All data were analyzed by ANOVA using the PROC MIXED of SAS with pig as the experimental unit. Inclusion of oligosaccharide-based polymer linearly increased (P &lt; 0.05) ADFI on d 0 to 5 PI, and feed efficiency on d 0 to 5 PI and d 5 to 11 PI (P = 0.07), compared with the control. Supplementation of AGP or oligosaccharide-based polymer reduced (P &lt; 0.01) frequency of diarrhea of pigs from d 0 to 11 PI. No differences were observed in overall growth performance and percentage of fecal β-hemolytic coliforms on d 8 PI among pigs in AGP and oligosaccharide-based polymer treatments. In conclusion, supplementation of oligosaccharide-based polymer enhanced feed efficiency and reduced diarrhea of weaned pigs infected with a pathogenic E. coli.

scholarly journals Antimicrobial Resistance Profiles of Escherichia coli from Diarrheic Weaned Piglets after the Ban on Antibiotic Growth Promoters in Feed

Antibiotics ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 755 ◽  
Author(s):  
Do Kyung-Hyo ◽  
Byun Jae-Won ◽  
Lee Wan-Kyu
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Diffusion Method ◽  
Growth Promoters ◽  
Disk Diffusion Method ◽  
Pathogenic Escherichia Coli ◽  
Before And After ◽  
Amoxicillin Clavulanic Acid ◽  
Antibiotic Growth Promoters ◽  
Polymerase Chain

This study aimed to survey the antimicrobial resistance profiles of 690 pathogenic Escherichia coli isolates obtained from Korean pigs with symptoms of enteric colibacillosis between 2007 and 2017, while assessing the change in antimicrobial resistance profiles before and after the ban on antibiotic growth promoters (AGPs). Following the Clinical and Laboratory Standards Institute guidelines, the antimicrobial resistance phenotype was analyzed through the disk diffusion method, and the genotype was analyzed by the polymerase chain reaction. After the ban on AGPs, resistance to gentamicin (from 68.8% to 39.0%), neomycin (from 84.9% to 57.8%), ciprofloxacin (from 49.5% to 39.6%), norfloxacin (from 46.8% to 37.3%), and amoxicillin/clavulanic acid (from 40.8% to 23.5%) decreased compared to before the ban. However, resistance to cephalothin (from 51.4% to 66.5%), cefepime (from 0.0% to 2.4%), and colistin (from 7.3% to 11.0%) had increased. We confirmed a high percentage of multidrug resistance before (95.0%) and after (96.6%) the ban on AGPs. The AmpC gene was the most prevalent from 2007 to 2017 (60.0%), followed by the blaTEM gene (55.5%). The blaTEM was prevalent before (2007–2011, 69.3%) and after (2012–2017, 49.2%) the ban on AGPs. These results provide data that can be used for the prevention and treatment of enteric colibacillosis.

Sign in / Sign up

Export Citation Format

Share Document